• Journal of Nutrition and Health
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• v.52 no.4
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• pp.323-331
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• 2019

Purpose: Glucocorticoids (GCs) are implicated in secondary osteoporosis, and the resulting fractures cause significant morbidity. Polyunsaturated fatty acids (PUFAs) play a vital role in bone metabolism. However, few trials have studied the impact of omega-3 PUFA-containing oils against GC-induced osteoporosis. Therefore, the present study was undertaken to determine whether supplementation with omega-3 PUFA-containing dietary oils such as fish oil, flaxseed oil or soybean oil can impede the development of GC-induced osteoporosis. Methods: The fatty acids (FAs) content of oils was determined using gas chromatography. Male rats were subdivided into 5 groups (8 rats each): normal control (balanced diet), prednisolone control (10 mg/kg prednisolone daily), soybean oil (prednisolone 10 mg/kg + soybean oil 7% w/w), flaxseed oil (prednisolone 10 mg/kg + flaxseed oil 7% w/w), and fish oil (from cod liver; prednisolone 10 mg/kg + fish oil 7% w/w). Results: The study data exhibited a significant depletion in bone mineral density (BMD) and femur mass in the prednisolone control compared to the normal control, accompanied with a marked decrease in the levels of plasma calcium and 1,25-$(OH)_2$-vitamin $D_3$, and elevated levels of C-terminal telopeptide (CTX), tumor necrosis factor-alpha (TNF-${\alpha}$) and malondialdehyde (MDA). Supplementation with fish oil, soybean oil or flaxseed oil helped to improve plasma calcium levels, and suppress oxidative stress and inflammatory markers. Additionally, bone resorption was suppressed as reflected by the decreased CTX levels. However, fish oil was more effective than the other two oils with a significant improvement in BMD and normal histological results compared to the normal control. Conclusion: This study demonstrated that supplementation with dietary oils containing omega-3 PUFAs such as fish oil, soybean oil or flaxseed oil can play a role in the prevention of bone loss and in the regulation of bone metabolism, especially fish oil which demonstrated a greater level of protection against GC-induced osteoporosis.

• Biomedical Science Letters
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• v.24 no.4
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• pp.357-364
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• 2018

Manganese (Mn) is used as main materials in various chemical processes of industry, but it suggested that Mn brings about its toxicant by fume or dust through respiratory system and skin barrier. Mn toxicant induces the loss of mental health and life quality by cerebrovascular and skin diseases. Nevertheless, it lefts much unknown on the mechanism and the effectively therapeutic methods about Mn toxicant. Therefore, this study was evaluated the cytotoxicity induced by manganese dioxide ($MnO_2$) in cultured NIH3T3 fibroblasts, and also, the correlation between $MnO_2$-induced cytotoxicity and oxidative stress was examined. While, the effect of Eclipta prostrata L. (EP) extract belong to Compositae was assessed against $MnO_2$-induced cytotoxicity in the view of antioxidative effect for searching the natural resources mitigating or preventing the $MnO_2$-induced cytotoxicity. In this study, $MnO_2$-induced cytotoxicity was revealed as mid-toxic by Borenfreud and Puerner's toxic criteria, and catalase (CAT), an antioxidant prevented $MnO_2$-induced cytotoxicity by the remarkable increase of cell viability in these cultures. While, in the protective effect of EP extract on $MnO_2$-induced cytotoxicity, EP extract effectively prevented the cytotoxicity induced by $MnO_2$ via antioxidative effects such as xanthine oxidase (XO) inhibitory ability and DPPH-radical scavenging ability. From the above results, EP extract showed the effective prevention against $MnO_2$-induced cytotoxicity correlated with oxidative stress by antioxidative effects. Conclusively, this study may be useful to research or development the alternatively therapeutic agent from natural resources like EP extract for the treatment of diseases resulted in oxidative stress.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.7
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• pp.863-869
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• 2018

Due to recent changes in the maritime traffic environment, naval warship accidents are constantly occurring. Especially in 2017, serious loss of life was caused by a US navy destroyer accident. The purpose of this study is to analyze the characteristics of naval warship accident cases and construct an accident scenario by using naval training materials, adjudication of naval warship accidents and US navy destroyer accident reports. Based on the surveyed data, the status of accidents was identified and cases were analyzed. We reproduced 17 accident cases in accordance with accident reproduction procedure and constructed naval warship accident scenarios. As a result of analyzing the CPA, TCPA and PARK model for risk, reproducing 17 naval ship accident cases, collision risk increased on average 5-6 minutes before an accident. The result of this study represents basic data for naval and simulation education materials, contributing to the prevention of marine accidents.

• Korean Journal of Acupuncture
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• v.36 no.2
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• pp.115-126
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• 2019

Objectives : Osteoporosis is a condition characterized by low bone mass and increased bone fragility. It has become a major problem of senior citizens. The purpose of this study is to experiment the effect of water extract of Forsythiae Fructus (wFF) on osteoclast differentiation; and the other purpose is to examine the effect of wFF on osteoporosis in ovariectomized rat. Methods : To investigate the effect of wFF on osteoclast differentiation and activity, RAW 264.7 cells were used. The number of TRAP positive cell, TRAP activity, pit area, mRNA expression of makers (RANK, TRAP, CA II, CTK, MMP-9, NFATc1, c-Fos), protein expression of makers (NFATc1, c-Fos) were investigated. For in vivo study, 40 female Sprague-Dawley (SD) rats were induced osteoporosis by ovariectomy (OVX) and then tested for anti-osteoporosis effect by administration of wFF. Results : wFF suppressed osteoclatogenesis, TRAP activity and pit area formation. Moreover, wFF decreased the expression of master differentiation factors (NFATc1, c-Fos) and also reduced the osteoclastogenesis-related markers (TRAP, CA II, CTK, MMP-9). These suggest that wFF inhibit osteoclasts differentiation and bone resorption. In the OVX rat model, wFF inhibited decreasing of BMD and trabecular area. Conclusions : Forsythiae Fructus should be effective for osteoporosis prevention and treatment.

• Journal of the Korean Geotechnical Society
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• v.35 no.1
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• pp.43-53
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• 2019

This paper described a result of finite element analysis considering sewerage damage scale and soil depth, in order to analyze quantitatively for cavity and relaxation zone of underground due to sewerage damage. The mechanical model, which was verified by previous studies, was applied to the finite element analysis. In addition, the mechanical behavior of the soil around the sewerage damage due to the soil loss was simulated by using the forced displacement. Based on finite element analysis results, characteristics of the void ratio distribution, ground subsidence, and shear stress distribution according to sewerage damage scale and soil depth were analyzed. And then, The boundaries of the underground cavity and relaxation zone were determined by using the shear stress reduction characteristics of the ground. Also, an occurrence scope of the cavity and relaxation zone was quantitatively evaluated by the change of sewerage damage scale and soil depth.

• Journal of the Korean Geosynthetics Society
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• v.18 no.3
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• pp.79-88
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• 2019

This study described a results of groundwater injection test in cavity, in order to evaluate characteristics of cavity expansion under pavement. That is, groundwater amount proportional to the cavity volume was injected into the generated cavity step by step, and then the cavity with the changed size was monitored as the injected groundwater was drained. The test result showed that the cavity volume by groundwater injection increased, and then it converged or decreased. This means that some of the relaxation soil around the cavity collapsed, and the fine-grained soils in some soils filled the void in the surrounding soils when the cavity is expanded by groundwater injection. The volume change and expansion characteristics of the cavity according to the groundwater injection step were analyzed. The result showed that the cavity extended laterally. Therefore, it was found that the cavity expansion is caused by the repetition of the relaxation soil collapse due to the groundwater flow and the loss of the collapsed soil below the cavity.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.20 no.1
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• pp.105-111
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• 2010

This paper proposes the algorithm which addresses the problem of detecting leak of defaced confidential documents from original confidential document. Generally, a confidential document is defaced into various forms by insiders and then they are trying to leak these defaced documents to outside. Traditional algorithms detecting leak of documents have low accuracy because they are based on similarity of two documents, which do not reflect various forms of defaced documents in detection. In order to overcome this problem, this paper proposes a novel v-SVDD algorithm which is based on SVDD, the novelty detection algorithm. The result of experiment shows that there is significant improvement m the accuracy of the v-SVDD in comparison with the traditional algorithms.

• Korean Journal of Pharmacognosy
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• v.51 no.4
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• pp.332-339
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• 2020

Lentinus edodes (LE) is the first medicinal macrofungus to enter the realm of modern biotechnology. Lentinus edodes is well-known to have anti-tumor, anti-inflammatory, and immunomodulatory effects. In this study, osteoporosis was induced by ovariectomy in female rats, and the prevention and treatment efficacy of the climacteric disease the postmenopausal type I pattern was examined using the experimental substance. Female rats were either sham-operated (sham; n = 5) or surgically ovariectomized (OVX; five animals per group) and then administered to OVX control, raloxifene hydrochloride 1 mg/kg/day, or LE (20 and 200 mg/kg/day) for 12 weeks. LE treatment suppressed the ovariectomy-induced reduction of bone mineral density in the entire tibia as well as its metaphysis with a decrease of serum osteocalcin and interleukin-6 levels. In addition, LE is suggested to elevate the serum levels of progesterone hormones and prevent bone loss in ovariectomized rats. In conclusion, LE 200 mg/kg/day may have inhibitory effects on osteoporosis in OVX rats.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.555-565
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• 2021

Bacteriophages infecting Acidovorax citrulli, the causal agent of bacterial fruit blotch, have been proven to be effective for the prevention and control of this disease. However, the occurrence of bacteriophage-resistant bacteria is one of hurdles in phage biocontrol and the understanding of phage resistance in this bacterium is an essential step. In this study, we aim to investigate possible phage resistance of A. citrulli and relationship between phage resistance and pathogenicity, and to isolate and characterize the genes involved in these phenomena. A phage-resistant and less-virulent mutant named as AC-17-G1 was isolated among 3,264 A. citrulli Tn5 mutants through serial spot assays and plaque assays followed by pathogenicity test using seed coating method. The mutant has the integrated Tn5 in the middle of a cupin protein gene. This mutant recovered its pathogenicity and phage sensitivity by complementation with corresponding wild-type gene. Site-directed mutation of this gene from wild-type by CRISPR/Cas9 system resulted in the loss of pathogenicity and acquisition of phage resistance. The growth of AC-17-G1 in King's B medium was much less than the wild-type, but the growth turned into normal in the medium supplemented with D-mannose 6-phosphate or D-fructose 6-phosphate indicating the cupin protein functions as a phosphomannos isomerase. Sodium dodecyl sulfa analysis of lipopolysaccharide (LPS) extracted from the mutant was smaller than that from wild-type. All these data suggest that the cupin protein is a phosphomannos isomerase involved in LPS synthesis, and LPS is an important determinant of pathogenicity and phage susceptibility of A. citrulli.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.34 no.6
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• pp.363-370
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• 2021

In this paper, we presented an optimized crack and flexural strength analysis of a glass-fiber reinforced polymer (GFRP) rebar, used as reinforcements for in-site railway concrete slabs. The insulation performance of a GFRP rebar has the advantage of avoiding the loss of signal current in an audio frequency (AF) track circuit. A full-scale experiment, and three-dimensional finite element simulation results were compared to validate our approaches. Parametric numerical results revealed that the diameters and arrangements of the GFRP rebar had a significant effect on the flexural strength and crack control performances of the concrete track slabs. The results of this study could serve as a benchmark for future guidelines in designing more efficient, and economical concrete slabs using the GFRP rebar.