• Journal of Chest Surgery
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• v.42 no.2
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• pp.141-147
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• 2009

Background: We performed this study to identify the tumor suppressor genes located in the long arm of chromosome 21 in non-small cell lung cancer. Material and Method: The genes of USP25 in 21q11.2, NCAM2, ADAMTS1 in 21q21.2, and Claudin-8 (CLDN8), Claudin-17 (CLDN17) and TIAM1 in 21q22.1 were investigated for their gene expressions, genetic alterations and promoter methylation. Result: The expressions of CLDN8 and CLDN17 were significantly decreased in 7 (L132, H157, H358, H522, H1299, H1703 and HCC2108) of 13 cell lines, and the expression of ADAMTS1 was also significantly reduced in 6 cell lines (A549, SW900, H1299, H1373, H1703 and H1793). There were no genetic alterations by PCR-SSCP and cDNA cloning in the cell lines with a decreased gene. In the cell lines with a decreased gene expression, the mRNA expression was increased significantly with treatment of 5-Aza-CdR. Conclusion: These results suggest that the ADMTS1, CLDN8 and CLDN17 may act as tumor suppressor genes.

• Journal of Pharmaceutical Investigation
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• v.40 no.3
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• pp.175-180
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• 2010

Albumin-modification has been viewed as one of the most effective ways of extending the short in vivo lifetimes of peptide drugs by delaying glomerular filtration. In this study, we describe a new type 2 anti-diabetic exendin-4 (Ex4) peptide derivative with significant binding ability to human serum albumin (HSA). This exendin-4 derivative consists of a 4-branched polyethylene glycol $(PEG)_{5k}$ (Mw: 20 kDa) modified with three stearylamines ($C_{18}-NH_2$) and one exendin-4 on its branches. PEG and stearylamine were selected to provide functionality to increase molecular size and bind to albumin, respectively. This derivative ($3C_{18}-4PEG_{5k}$-Ex4) was shown to have larger molecular size (Ca. 152 kDa) than actual (25.0 kDa) when subjected to size-exclusion chromatography, and the fluorescein-tagged $3C_{18}-4PEG_{5k}$-Ex4 displayed significant binding to the HSA-immobilized Sepharose CL-4B resin using confocal laser scanning microscopy. Furthermore, $3C_{18}-4PEG_{5k}$-Ex4 was found to have acceptable anti-hyperglycemic efficacy via three consecutive oral glucose tolerance testings (OGTT) in fasted type 2 diabetic db/db mice. The $HD_{total}$ value ($57.6{\pm}12.3%$) of $3C_{18}-4PEG_{5k}$-Ex4 at a 50 nmol/kg dose was 2-fold greater than that ($31.0{\pm}8.7%$) of native exendin-4 in non-fasted db/db mice. Especially, the blood glucose levels in the mice group treated with $3C_{18}-4PEG_{5k}$-Ex4 did not rebound to ~150 mg/dL until 24 h after the injection, which obviously shows the extended hypoglycemia. We believe that this derivative has great pharmaceutical potential as a novel long-acting type 2 anti-diabetic injection treatment.

• Journal of Korean Orthopaedic Sports Medicine
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• v.2 no.1
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• pp.62-70
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• 2003

Purpose: We examined the kinematics and kinetics of the shoulder in youth baseball pitchers in light of the mechanisms of development of little league shoulder and humeral retrotorsion. Materials and Methods: The joint kinematics and the net force and torque acting on the humerus were calculated in fourteen youth pitchers throwing in a simulated game. Results: The major force component acting on the humerus was a tensile force of 378$\pm$81 N that peaked just after ball release. The predominant torque on the humerus was an external rotation torque about the long axis of the humerus. This torque reached a peak value of 35.3$\pm$6.7 Nm about 73$\%$through the pitching motion. This torque is approximately 66$\%$ of the torque required to fracture of the adult humerus. Conclusions: The direction of the humeral torque was consistent with the development of increased humeral retrotorsion in the throwing arm. Shear stress arising from the high torque during the late cocking phase likely leads to deformation the relatively weak proximal humeral epiphysis. The external rotation torque applied to the humerus during the pitch also agrees with the proposed mechanism for development little league shoulder, which has been hypothesized to be due to rotational stresses acting on the epiphysis during the throwing motion.

• Journal of Korean Orthopaedic Sports Medicine
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• v.4 no.1
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• pp.43-48
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• 2005

Purpose: The purpose of this study is to assess the result of conservative treatment in displaced radial head fracture. Materials and Methods: We analyzed 15 cases of displaced radial head fracture who were treated conservatively from April 1992 to May 2002. Follow-up period was more than 1 year. They were all males and the average age of patients was 20.2($11{\sim}24$) years. The average follow-up period was 13.4($12{\sim}15$) months. They were able to do active pronation and supination more than 70 degrees initially, who were applied long arm splint less than 1 week. The functional rating index modified after B.F. Morrey and no further displacement and union state of fragment in simple X-ray were used in follow-up assessment. Results: On the basis of the functional rating index modified after B.F. Morrey that included elements of pain, motion, strength, and stability, the results were 8 excellent, 7 good in patients who were treated conservatively, who had union and no further displacement of fragment in follow-up. Conclusion: We conclude that if they are able to do active pronation and supination more than 70 degrees initially, we take them to do early range of motion exercise after subsiding swelling, which can restrict limitation of range of motion exercise and return to the living early.

• The Korean Journal of Physiology
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• v.4 no.2
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• pp.37-44
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• 1970

Human body volumes were calculated from the measurments of body height and body weight. Equations Suited to express the relations of height, weight, and surface area to show the body volume were derived from the body volume measurements by means of underwater. weighing method. Underwater body weights were corrected for the residual volume of long obtained by the Rahn's three breath method. Underwater weighing was performed on 173 male subjects aged between 13 and 51 years. Subjects were divided into 4 age groups, namely, 13-16 years group of 47 subjects, 16-19 years group of 46 subjects, adult group aged between 22 and 38 years comprising 45 subjects, and middle-aged group (40-51 years) of 35 subjects. The group division was made on .the basis of physical growth and development. The following results were obtained. 1. Body height (H, cm), body weight (W, kg), body surface area $S,\; m^{2})$, and body volume (V, liter.) interrelated closely. V/S showed a high correlation with W/H and the coefficient of correlation was r=0.97 irrespective of age group differences of the subjects. The coefficients of correlation between V/S and W/H in the total mate subjects as a single group was r=1.983. Subsequently the following regression equation was obtained. V = S X (54.84 W/H + 14.08) The agreement of body volume values obtained by the calculation and underwater weighing in the total subject group was better than that of the separate age group division. 2. The calculated values of body volume were: 40.4 l (euiqvalent to the body density value of 1.0562 kg/1) in 13-16 years group; 52.0 l (equivalent to density value of 1.0723 kg/l) in 16-19 years group; 55.3 l (equivalent to density value of 1.0570 kg/l) In the adult group; and 54. 0 l (equivalent to density value of 1.074 kg/l) in the middle-age group. The mean deviation of calculated from the measured volume value ranged between ${\pm}0.55$ and ${\pm}0.81$ liters. 3. The correlation between V/S and mean skinfold thickness of 4 sites (arm, back, iliac and chest) was high, namely, the coefficient of correlation was r=0.656. The coefficients of correlation between V./S and the $R\"{o}hrer$ index ranged between r=0.668 and r=0.810 affected by the difference in group age of the subject. The body volume (V) alone correlated poorly than V/S with mean skinfold thickness (r=0.606) and the $R\"{o}hrer$ index (r ranged between 0.274 and 0.588).

• Korean Journal of Applied Biomechanics
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• v.14 no.2
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• pp.27-40
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• 2004

The objective of this study is to identify the kinematic variables of giant swing backward to handstand as well as individual variations of each athlete performing this skill, which in turn will provide the basis for developing suitable training methods and for improving athlete's performance in actual games. For this end, 3 male athletes, members of the national team, who are in ${\Box}{\Box}H{\Box}{\Box}$ University, have been randomly chosen and their giant swing backward to handstand performance was recorded using two digital cameras and analyzed in 3 dimensional graphics. This study came to the following conclusion. 1. Proper time allocation for giant swing backward to handstand are: Phase 1 should provide enough time to attain energy for swing track of a grand round movement. The phase 3 is to throw the body up high in the air and stay in the air as long as possible to smoothen up the transition to the next stage and the phase 4 should be kept short with the moment arm coefficient of the body reduced. 2. As for appropriate changes of locations of body center, the phase 1 should be comprised of horizontal, perpendicular, compositional to make up a big rotational radius. Up to the Phase 3 the changes of displacements of vertical locations should be a good scale and athlete's body should go up high quickly to increase the perpendicular climbing power 3. When it comes to the speed changes of body center, the vertical and horizontal speed should be spurred by the reaction of the body in Phase 2 and Phase 3. In the Phase 4, fast vertical speed throws the body center up high to ensure enough time for in-the-air movement. 4. The changes of angles of body center are: in Phase 2, shoulder joint is stretching and coxa should be curved up to utilize the body reaction. In the Phase 4, shoulder joint and coxa should be stretched out to get the body center as high as possible in the air for stable landing. 5. The speeds of changes in joints angles are: in the Phase 2 should have the speed of angles of shoulder joints increase to get the body up in the air as quickly as possible. The Phase 3 should have the speed of angles in shoulder joint slow down, while putting the angles of a knee joint up to speed as quickly as possible to ensure enough time for in-the-air movement.

• The Journal of Korean Academy of Sensory Integration
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• v.14 no.2
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• pp.1-10
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• 2016

Objective: This study aims investigating the effects of functional electrical stimulation (FES) on hand function of children with spastic cerebral palsy. Methods: The participants of this study are 20 children with spastic cerebral palsy aged between 11 to 16 years old. All the subjects underwent 24 sessions of FES during 8 weeks. Each FES was 20 minutes per session, and 3 sessions of FES were provided in a week. Measurements used to assess hand function are Jebsen Hand Function Test, Modified Ashworth Scale and 3D Motion Analysis. After establishment of the baseline for each client by all the measurements, reevaluations were performed every 2 weeks using Jebsen Hand Function Test and the Modified Ashworth Scale. The 3D Motion Analysis was performed only before- and after the 8 weeks of FES treatment. Results: After the FES, there was significant decrease in completed time for the all 6 subtasks of Jebsen Hand Function Test were (p<.05) and also significant decrease in spasticity score of Modified Ashworth Scale as well (p<.05). 3D Motion Analysis showed that the hand tapping and the finger tapping has been significantly improved (p<.05), and the pronation-supination movement of lower arm has been significantly improved as well. Conclusion: Based on the results of this study, it is evidenced that functional electrical stimulation is effective treatment for hand function of children with cerebral palsy. For future research, it is recommended to examine various protocols of FES including impact of long-term application.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.1
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• pp.57-65
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• 1997

Cytogenetic observations of loss of the distal portion of the Y chromosome long arm were found to be associated with disrupted spermatogenesis. The existence of a gene involved in the regulation of spermatogenesis, the azoospermia factor (AZF), was postulated. In this study, we screened the AZF region including DAZ and DAZH genes and observed the expression pattern of DAZ and DAZH transcript in infertile men with azoospermia and oligospermia by using a sequence-tagged site (STS)-based PCR method. PCR primers were synthesized for 11 STSs that span Yq interval 6, SRY, DAZ, and DAZH, functional DAZ homologue on chromosome 3. Microdeletions were detected in 4/32 (12.5%) azoospermic men and 1/11 (9%) severe oligospermic men. Only 2 of 5 patients had microdeletions of Yq that contained the DAZ gene, whereas the other 3 patients had deletions extending from intervals 5L-6F proximal to the DAZ gene on Yq. Testis biopsies of the azoospermic patients revealed a variety from Sertoli cell-only syndrome to testicular maturation arrest. Of 4 men with clinical data available, average testis size was R: 13.8 cc, L: 13.8 cc, serum T was $4.0{\pm}1.25$ ng/ml, LH was $3.63{\pm}1.90$ mIU/ml, and FSH was $8.85{\pm}5.13$ mIU/ml. These values did not differ significantly from the remainder of the patients tested. We could not observed the DAZ transcript in 2 patients, who have no mature spermatozoa. In 11.6% of patients microdeletions of the AZF could be detected. These deletions in the AZF region seem to be involved causing spermatogenic failure. But the frequency of microdeletions proximal to DAZ suggests that DAZ is not the only gene associated with spermatogenic failure.

• Journal of Ginseng Research
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• v.32 no.4
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• pp.341-346
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• 2008

In the present study, we assessed the effects of white ginseng and red ginseng extract on the learning and memory impairments induced by scopolamine. The cognition-enhancing effect of ginseng extracts was investigated using the Morris water maze and Y-maze test. Drug-induced amnesia was induced by treating animals with scopolamine (2 mg/kg, i.p.), an antagonist of muscarinic acetylcholine (ACh) receptor. Tacrine was used a positive control. Ginseng extract (200 mg/kg, p.o.), tacrine (10 mg/kg, p.o.) administration significantly reduced the escape latency during training in the Morris water maze (p<0.05). At the probe trial session, scopolamine significantly increased the escape latency on day 5 in comparison with control (p<0.01). The effect of ginseng extracts on spontaneous alternation in Y-maze was similar to that of scopolamine treated group. In addition, numbers of arm entries were similar in all experimental groups. Moreover, red ginseng extract significantly inhibited acetylcholinesterase activity in the cortex and serum (p<0.05). Brain ACh contents of ginseng extract treated groups increased more than that of scopolamine group, which did not show statistically significant. These results suggest that ginseng extract may be useful for the treatment of cognitive impairment.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.286-293
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• 2012

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles ($L^0$, $L^1$, $L^2$, $L^3$, and $L^4$) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, $L^0L^0$), C. annuum Tisana ($L^1L^1$), C. annuum Criollo de Morelos 334 (CM334, $L^2L^2$), Capsicum chinense PI 159236 ($L^3L^3$), and Capsicum chacoense PI 260429 ($L^4L^4$). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the $L^3$-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 $F_1$ hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes ($P_0$, $P_1$, and $P_{1,2$). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.