• Title/Summary/Keyword: locus

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Analysis of Mating System in Lentinula edodes and Development of Mating Type-Specific Markers

  • Ha, Byung-Suk;Kim, Sinil;Ro, Hyeon-Su
    • 한국균학회소식:학술대회논문집
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    • 2014.10a
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    • pp.42-42
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    • 2014
  • Mating of tetrapolar mushrooms is regulated by to chromosomal loci, A and B. A locus contains A gene that expresses a homeodomain protein whereas B locus contains multiple pheromones and receptor genes. In order to characterize the mating loci in Korean cultivated strains of Lentinula edodes, one hundred monokaryotic myclelia were isolated from the basidiospores of cultivated strains, including Cham-A-Ram, Sanjo701, and Sanjo707. Both mating loci were amplified using primer sets targeting conserved sequence regions for homeodomain (HD), pheromone, and receptor genes. Subsequent sequence analysis revealed that the Korean strains contained significant variations in the homeodomain of A locus, even within the same A1 or A2 mating type. Similarly, B locus was also highly diversified in the sequences of pheromones and receptors as well as gene organization. These results enabled us to design mating type-specific probes which can distinguish mating type of each strain. The specificity was confirmed by between intra- and inter-strain mating experiment.

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The School-Age Children's Copings: Cross-Situational Consistency and Internal/External Locus Control (학령기 아동의 스트레스 대처행동의 상황간 일관성 및 내외 통제소재에 따른 대처행동)

  • 민하영;유안진
    • Journal of the Korean Home Economics Association
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    • v.38 no.10
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    • pp.15-26
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    • 2000
  • This study was to investigate cross-situational consistency in copings and copings according to an internal/external locus of control. The subjects were 489 the sixth grade children selected from seven elementary schools in Seoul. The data were analyzed using Pearson's correlation and ANOVA Major findings were that (1) Correlations among corresponding copings across stress situations-such as parents, friend-alienation, friend-conflict, and loaming related stress situations- ranged from r=.27 to r=.67, reflecting cross-situational consistency in copings. (2) Generally, children with an internal locus of control used more active coping behaviors, but used less passive and aggressive coping behaviors than children with an external locus of control in stress situations.

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A Study on the Locus of Control and Environmental Behavior of Undergraduate Students (대학생들의 통제성향과 환경행동에 관한 연구)

  • Oh, Hae-Sub
    • Journal of Agricultural Extension & Community Development
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    • v.3 no.2
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    • pp.265-271
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    • 1996
  • Locals of Control is considered one of the main imporment factors on responsible environmental behaviors(REB). It is defined as an individual's perception of his or ha ability to bring about change through his a her behavior. This psychological construct is divided in two, external and internal. Internal locus of control(ILOC) is imporment as predictor for responsible environmental behaviors. This study was conducted to investigate the Locus of Control and relations with REB in Undergraduate Students. The data were collected mainly through questionnaires and a total of 157 responded questionnaires was analyzed for the study. The statistical methods used in this study were frequency, percentile and correlation. The major findings of this study were (1) Most of undergraduate students had internal locus of control(75.2%). (2) According to region, rural students showed high levels in ILOC and REB.

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Linear Structural Relationships in Children's School Maladjustment and Related Variables (아동의 학교부적응에 영향을 미치는 관련 변인의 구조분석)

  • Lee, Kyeong Hwa;Son, Won Kyoung
    • Korean Journal of Child Studies
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    • v.26 no.4
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    • pp.157-171
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    • 2005
  • Causal relationships between school maladjustment and related variables were examined in 371 5th grade elementary school students. School maladjustment was ascertained by revised the School Adjustment Test(Lim, 1993; Song, 1999). After analysis of differences in internal locus of control, academic stress, and academic achievement between the school maladjusted and adjusted groups, the study built a structural equation model of school maladjustment-related variables goodness of fit was tested with LISREL 8 for Windows. Significant differences were found in internal locus of control, academic stress, and academic achievement by level of school maladjustment. Internal locus of control, academic stress, and academic achievement both directly and/or indirectly influenced school maladjustment. These variables explained 64% of the variances in school maladjustment.

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Structural Equation Model of Middle School Students' Stress, locus of control, and Adjustment (중학생의 스트레스 및 통제소재와 학교적응의 구조분석)

  • Lee, Kyeong-Hwa;Jung, Hye-Young
    • Journal of Fisheries and Marine Sciences Education
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    • v.19 no.1
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    • pp.8-18
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    • 2007
  • In order to define their relative influence, this study analyzed causal relationships between school adjustment of middle school students and such related variables as locus of control and stress, including clarification of other significant variables affecting school adjustment. Participants were a sample of 331 students from 3 middle schools in Busan. Correlation, analysis of covariance structure modeling, and model-fitness tests were applied to the data. Results indicated that stress on the school, home, social relationships, and self has the more significant influence on the school adjustment, and the locus of control has relatively low influence.

Korean-Chinese Adolescents′ Acculturation and Adjustment in Shenyang and Harbin (문화접변 유형에 따른 조선족 청소년의 적응 : 심양과 할빈을 중심으로)

  • 조복희;박태수;한세영
    • Journal of Families and Better Life
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    • v.22 no.5
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    • pp.113-122
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    • 2004
  • This study examined. the acculturation type and adjustment of 914 Korean-Chinese adolescents living in Shenyang and Harbin, China. Adolescents completed a questionnaire consisted of items regarding acculturation, school adjustment, expectation of education, and internal locus of control: The results are summarized as follows: 1) Adolescents in Shenyang spoke more chinese and showed lower internal locus of control than adolescents in Harbin. 2) Adolescents in Shenyang showed more integration and assimilation types, while adoelscents in Harbin showed more separation type. 3) Adolescents in Shenyang showed difference in their adjustment across acculturation types, while adolescents in Harbin did not. In Shenyang, adolescents of integration and assimilation showed higher expectation of education, and adolescents of separation revealed higher internal locus of control.

A Hybrid DTC-DSC Drive for High Performance Induction Motor Control

  • Jidin, Auzani;Idris, Nik Rumzi Nik;Yatim, Abdul Halim Mohamed;Sutikno, Tole;Elbuluk, Malik E.
    • Journal of Power Electronics
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    • v.11 no.5
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    • pp.704-712
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    • 2011
  • This paper describes a hybrid induction motor drive system incorporating DTC-hysteresis and Direct Self Control (DSC) schemes to achieve excellent dynamic performance. The control scheme is switched from a circular to a hexagonal flux locus whenever a dynamic condition is encountered. On the other hand, when the motor operates under steady state conditions, a circular flux locus is used. Without major modifications to the simple structure of a basic DTC, hexagonal flux locus operation is established by modifying the flux error status, before it is fed to the look-up table. The feasibility of the proposed hybrid scheme to achieve excellent control performance is verified by experimental results.

(CA/GT)n Simple Sequence Repeat DNA Polymorphism in Chlamydomonas reinhardtii (녹조류 Chlamydomonas reinhardtii의 (CA/GT)n Simple Sequence Repeat DNA 다형현상)

  • ;;Marvin W. FAWLEY
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.2
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    • pp.113-117
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    • 1997
  • Simple sequence repeats (SSR) are widely dispersed throughout eukaryotic genomes, highly polymorphic, and easily typed using polymerase chain reaction (PCR). The objective of this study was to determine the polymorphism of different Chlamydomonas reinhartdtii strains and to determine the mode of inheritance of the SSR locus in Chlamydomonas. A genomic DNA library of C. reinhardtii was constructed and screened with a radiolabeled $(AC)_{11}$ probe for the selection of (CA/GT)n repeat clone. Selected clone was seqeuenced, and PCR primer set flanking (CA/GT)n sequence was constructed. PCR was used to specifically amplify the SSR locus from multiple isolates of C. reinhardtii. The locus was polymorphic in some of the C. reinhardtii isolates. However, the locus was amplified only 4 of 6 isolates of C. reinhardtii, not in other 2 isolates of C. reinhardtii, suggesting that this locus is not extensively conserved. A simple Mendelian inheritance pattern was found, which showed 2:2 segregation in the tetrads resulting from a cross between C. reinhardtii and C. smithii. Our results suggest that this simple sequence repeat DNA polymorphism will be useful for identity testing, population studies, linkage analysis, and genome mapping in Chlamydomonas.

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S-haplotypes and Genetic Diversity in 'Danji' Radish (Raphanus sativus L. var. hortensis)

  • Ahn, Yulkyun;Kim, Hyukjun;Han, Dongyeop;Park, Younghoon
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.210-216
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    • 2014
  • The distribution of S-haplotypes and genetic relationships were evaluated for 47 accessions of 'Danji' radish (Raphanus sativus L. var. hortensis Baker f. gigantissimus Makino) originating from Jeju Island in South Korea. A total of 22 S-haplotype-specific SCAR markers for the S locus glycoprotein (SLG) and S receptor kinase (SRK) loci were tested, and six primer sets amplified locus-specific PCR fragments from at least one 'Danji' radish accession. S5 and S21 alleles atthe SLG locus were the most frequently distributed, and detected from 87.5% and 64.6% of the accessions, respectively. The frequency of the class-II haplotype at the SLG locus was 75%, more frequent than the class-I haplotype. The S23 allele at the SRK locus was detected from 7 accessions. Grouping of the accessions based on S-allele composition revealed three major groups, while 8 accessions showed a unique allelic composition. The genetic diversity of 47 'Danji' radishes and 1 'Gwandong' radish were also evaluated with 38 RAPD primers. A total of 312 bands were scored, and showed that 138 bands (44.2%) were monomorphic among the accessions, whereas 174 (55.8%) bands were polymorphic. Polymorphism rates ranged from 0.2 to 1.0, indicating significant variations in detecting polymorphism across RAPD primers. The genetic similarity coefficients among all pairs of the 48accessions varied from 0.62 to 0.93, and 42% of the comparisons exhibited values higher than 0.85. All the cultivars could be distinguished based on the DNA fingerprints revealed by RAPD. The comparisons between the dendrograms based on S-haplotypes and RAPDs indicate an unrelated and sporadic distribution for several accessions; however, there was a tendency for accessions with the same S-allelic composition to group into the same cluster.