A 98 d feeding trial carried out to study liveweight change and rumen metabolites in heifers weighing initially 275 kg and given either untreated or ammoniated rice straw supplemented with 0, 0.4, 0.8 or 1.2 kg protein meal consisting of cottonseed meal (60). All 32 animals received 0.6 kg rice polishings/hd/d and had continuous access to molasses/urea block-licks containing 15% urea. The effects on growth rates of treatment of the straw with ammonia and of supplementation with bypass protein were additive. The heifers fed ammoniated straw grew 267 g/hd/d (p<0.001) faster and consumed 11% (p<0.05) more straw than the heifers on untreated straw. The mean growth response to bypass protein was 0.37 kg gain/kg protein meal supplied. Supplementation with protein meal tended (p=0.06) to depress intake of straw, but straw intakes of the unsupplemented groups were high. Small changes in the composition of the block-licks that were fed throughout the feeding trial led to changes in block intake and in intake of untreated straw. Increasing quantities of protein meal fed were associated with linear increase in concentrations of ammonia (p<0.05) and in molar percentages of iso-butyrate (p<0.01), iso-valerate (p<0.01) and valerate (p<0.01) in the rumen fluid of the heifers on a basal diet of untreated straw. However, in the rumen fluid of the heifers given ammoniated straw, the levels of these metabolities were not affected by the quantity of protein meal given.
Four experimental diets were formulated to contain 700 g/kg cereal with decreasing levels of wheat:barley inclusion. Diet 1 contained 700 g/kg wheat, diet 2 contained 600 g/kg wheat and 100 g/kg barley, diet 3 contained 500 g/kg wheat and 200 g/kg barley and diet 4 contained 400 g/kg wheat and 300 g/kg barley. The diets were offered to pigs on three trials to investigate effects on the performance of individually (n = 72) and group housed (n = 480) pigs and on nutrient digestibility in pigs housed in metabolizm crates (n = 24). Performance was assessed from 10 wks of age until slaughter and carcass characteristics were measured. For the group performance study, one pig from each pen (in total 24) at 10, 15 wks and at finish were slaughtered to ascertain scores for stomach ulceration, stomach weights and intestinal length. Level of wheat inclusion did not significantly (p>0.05) affect liveweight gain (LWG) or feed conversion ratio (FCR). Feed intake was lowest (p<0.05) for individually housed pigs offered diets containing 700 g/kg wheat during the 10-15 wk period, which indicated that individually housed pigs attempted to eat to a constant energy intake. There was little evidence of stomach ulceration across treatments and increasing wheat inclusion had no detrimental effect. Higher levels of wheat inclusion tended to increase backfat depth at the $P_2$ position which could lead to increasing grading penalties in a commercial situation although more research is required in this area. Increasing level of wheat inclusion increased digestible energy (DE) content but the lack of effect on FCR and killing out percentage indicated that utilization of energy from barley and wheat was similar. Digestibility coefficients increased linearly with increasing wheat content, which can be attributed to the lower level of fibre and higher level of starch in wheat compared with barley.
Garcia, Elisa Mariana;Lopez, Agustin;Zimerman, Maria;Hernandez, Olegario;Arroquy, Jose Ignacio;Nazareno, Monica Azucena
Asian-Australasian Journal of Animal Sciences
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제32권9호
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pp.1439-1447
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2019
Objective: The aim of this study was to evaluate the effect of dietary incorporation of tanninrich woody species on meat oxidative stability, carcass traits and meat quality in goats. Methods: Two tannin-rich species were tested using a three-treatments feeding trial, where treatments consisted of: Larrea divaricata and Acacia aroma both at 12.5% in dry matter basis of the diet and a control diet (alfalfa hay). All feeding diets were iso-protein and iso-energy. Carcass conformation, carcass compactness, carcass fatness and subcutaneous fat deposition were evaluated. Intake, liveweigh, Longissimus thoracis et lumborum muscles of goats were analyzed in order to evaluate quality parameters such as pH value, instrumental color evaluation, water holding capacity, total phenolic content, antioxidant activity, meat oxidative stability and fatty acid profiles in meat. Results: Feed intake, liveweight gain, carcass, and meat traits did not differ among treatments. Changes in meat lipid profile among treatments were observed for oleic and elaidic acid contents. Meat total phenolic content and antioxidant activity did not differ among treatments; although, meat oxidative status after storage at room temperature, as well as under refrigerated and frozen conditions were different between control and both supplemented groups. Conclusion: The inclusion of Acacia aroma and Larrea divaricata leaves in goat diet enhanced meat oxidative stability. Modulation of the ruminal biohydrogenation of fatty acids produced by condensed tannins of these plant species need to be further investigated.
Eighteen growing male Murrah buffalo (Bubalus bubalis) calves were divided into three groups consisting of six animals each and fed three urea ammoniated wheat straw (UAS) -based rations supplemented with concentrate mixtures (roughage: concentrate ratio 58:42) containing deoiled ground nut cake, GNC (8%), formaldehyde treated GNC (8%) or fish meal (8%) to undertake comparative evaluation of these rations in terms of their $CH_4$ production and growth (285 d duration) potential. A digestibility trial (10 d duration) was followed by a comparative calorimetric study in respiration chamber. Dry matter (DM) intake (84.3 to $89.3g/kg\;W^{0.75}d^{-1}$) did not differ between treatments. The digestibility coefficient of DM, organic matter (OM), crude protein (CP), neutral and acid detergent fiber did not differ significantly in different diets. Urinary energy loss as a percent of gross energy (GE) was not affected by diets. Average values of $CH_4$ production were 84.3, 77.6 and 99.1 g/d and $CH_4$ energy losses as percent of gross energy were 5.7, 5.2 and 6.1 percent on .GNC, formaldehyde treated GNC and fishmeal, respectively, and did not differ significantly. When expressed per unit of digestible OM intake, $CH_4$ production (g) was lower (p<0.05) on formaldehyde treated GNC (30.6) than on untreated GNC (30.6) and fish meal (31.9). Total ME intake and heat production were similar and hence the energy balances on different diets were similar. Nutritive value of rations in terms of digestible CP and ME were similar. Average daily gain calculated on the basis of regression of fortnights on cumulative liveweight gain in calves fed on concentrate containing unprotected GNC, protected GNC and fish meal were 437.1, 483.9 and 481.6 g, respectively. This indicated that the intake of energy was sufficient to meet the requirement of calves growing at 400 g per d. However, CP intake was around 150% of the stipulated standard (Kearl, 1982). Feed conversion ratios on unprotected GNC, protected GNC and fish meal were 11.60, 11.10 and 10.4 respectively. It was concluded that because significantly (p<0.05) low $CH_4$ is produced on protected GNC (8%), it is very good and sustainable protein source in comparison to poor quality fish meal and untreated GNC to be used in concentrate mixture for supplementing UAS-based diets.
A growth trial (Expt. 1) and an in vitro fermentation experiment (Expt. 2) were conducted to determine the response of growth performance, cecal fermentation characteristics and in vitro gas production to incremental levels of substitution of digestible fiber for lignified fiber in the diet of weaned rabbits. Three diets, formulated by substituting soyhulls (SH; used as digestible fiber source) for soybean straw (used as lignified fiber source) at substitution levels of 0, 25 and 50%, were used in a factorial design. In the growth trial (Expt. 1), increasing levels of SH substitution resulted in a quadratic increase in daily body weight gain rate (p<0.04) and feed conversion efficiency (p<0.02), but in a numerical decrease in dietary DM intake (p=0.15). When SH were included in the diet at 25% substitution level, rabbits had the highest rate of liveweight gain and feed conversion efficiency. As SH substitution level increased, pH values and ammonia-N of cecal contents linearly (p<0.001) decreased, but total VFA concentration linearly (p<0.03) increased. With incremental levels of SH substitution, the percentage of acetate and butyrate linearly (p<0.05) reduced, but the percentage of propionate and minor acids linearly (p<0.03) increased. Increasing the SH substitution levels tended to increase incidence of diarrhea. In the in vitro fermentation experiment (Expt. 2), regardless of origin of substrates fermented, increasing SH substitution level resulted in increased maximal gas production (p<0.001) and shortened gas production lag time, but had no effect on gas production rate (p>0.2). These observations suggest that incrementally feeding SH to rabbits could stimulate their cecal microbial activity, allowing cecal fermentation to shift towards favoring fiber digestion. In conclusion, digestible fiber from soyhulls may partially substitute for more lignified fiber, soybean straw, without having an adverse effect on cecal fermentative and microbial activity and growth performance. For growing rabbits, about 73% of total dietary NDF should be supplied by effective NDF, the remainder could come from digestible NDF, such as soyhulls.
Objective: Mulberry (Morus alba L.) is a cultivated shrub grown widely in the sub-tropical and tropical areas. It has been shown that mulberry leaf contains high levels of protein while having polyphenols as phytonutrients. Therefore, it is important to conduct an experiment to assess potential toxic level from mulberry on behavior, blood hematological and coagulation parameter using Sprague-Dawley (SD) rats. Methods: Both male and female SD rats were given an intragastric administration of respective treatments of mulberry leaf intakes (control, low and high levels). Parameters of feed intake, hematological and coagulation of blood parameters, as well as liveweight changes were taken during the 7 d of adaptation, 28 d of treatment exposure, and 14 d of recovery periods, respectively. All treatment data were statistically analyzed using analysis of variance of SPSS17.0 for Windows Statistical Software following the Randomized complete block design with sex as a block. Results: Most of the parameters of the physical symptoms of the SD rats, were not significantly different (p>0.05) when compared with that of the control group. Those which remain unchanged in each dose group were, body weight (BW) gain, feed intake, the hematology and coagulation indexes. Although, there were a few individual indicators that were abnormal, but the overall physiological appearance of the rats were normal. Conclusion: Results under this experiment revealed that most hematological and coagulation parameters of the SD rats in both male and female were normal, although the weight gain of female rats in high-dose group was significantly reduced than those of the male rats. Under this study, the use of mulberry leaf up to 2 g/kg BW did not result in abnormal phenomenon in the SD rats. These findings would offer useful information for further in vivo feeding trials in animals to extensively use of mulberry leaf to improve animal production, particularly in P.R. China.
본 연구는 잔반을 가축의 사료로 이용하기 위하여 Aspergillus와 Bacillus가 주요 미생물인 접종물을 이용하여 50kg 용량의 발효장치에서 발효시킨 잔반사료의 사료적 가치를 조사하기 위하여 시판 흰쥐사료와 10% 및 20% 대체하여 처리당 세번 반복 반복당 4마리씩 총 36마리의 흰쥐를 완전임의 배치법에 의하여 배치한 후 증체량, 사료섭취량, 사료요구율 및 소화율을 측정하였다. 발효전 잔반의 조성분 함량은 건물 19.0%. 조회분 18.0%, 조단백질 21.7%, 조섬유 18.1% 및 조지방 15.4%였고 제조된 잔반사료의 화학적 조성은 건물 88.5%. 조회분 13.0%, 조단백질 20.8%, 조섬유 13.6% 및 조지방 9.2% 였다. 생체중은 실험 개시 1주 후에는 잔반사료 급여량이 증가할수록 낮게 나타났으나(p<.05), 2주 및 3주 후에는 20% 대체구가 대조구 및 10% 대체구에 비하여 약 82%의 생체중을 나타냈다(p<.05).증체량은 실험개시 후1주 동안 10% 및 20% 대체구가 대조구보다 각각 27.5%와 47.3% 낮은 증체량을 나타냈으며(p<.05), 1주에서 2주 사이에는 10% 대체구가 다른 처리구보다 높았으나(p<.05), 2주에서 3주 사이에는 20% 대체구의 증체량이 다른 처리구보다 낮게 나타났다(p<.05). 총 증체량은 대조구와 10% 대체구는 차이가 없었으나 20% 대체구는 다른 처리구에 비하여 약 24% 낮은 증체량을 나타냈다(p<.05). 사료 섭취량은 실험 후 1주 동안은 대조구에 비하여 20% 대체구가 낮았으나(p<.05), 2주에서 3주 동안에는 잔반사료 대체수준이 높을수록 사료 섭취량이 증가하는 경향을 나타냈으며(p<.05), 총 사료 섭취량은 잔반사료 20% 대체구가 대조구에 비하여 높게 나타났다(P<.05). 사료 요구율은 처음 1주 동안은 잔반사료 대체수준이 증가할수록 높았으나(p<.05), 실험후 2주 및 3주째 까지는 20% 대체구가 대조구에 비하여 높았다(p<.05). 실험사료의 건물 소화율(%)은 대조구와 잔반사료 10% 대체구에 비하여 20% 대체구가 낮게 나타났으며 (p<.05), 유기물 소화율(%)은 대조구가 잔반사료 20% 대체구에 비하여 높게 나타났다(p<.05).
Nutritional evaluation of some agro-industrial byproducts available in Samoa [dry brewers' grains (DBG), cocoa shell (CS), cocoa dust (CD) and desiccated coconut waste meal (DCWM)] available in Samoa was carried out using both the in vivo and in vitro techniques. In the in vivo study 24 Anglo-nubian goats were offered by-products with other feed ingredients to compound four different diets. The goats were randomly allocated to 4 diets on the basis of liveweight (18.7-0.3kg). The ADF content of the byproducts followed a similar trend to NDF. The byproducts have a high content of organic matter (91.0-95.4%). Gross energy (GE) content was higher in DCWM (25.1 MJ/kg DM), closely followed by CD (23.2 MJ/kg DM). Concentrate intake was significantly different (p<0.05) among the goats. Average daily live weight gains were 105, 92, 88 and 97 g/goat/day for DBG, CS, CD and DCWM, respectively. Daily live weight gains were higher (p<0.05) in the goats that received DBG, while the least gain was obtained in the goats that received CS byproduct diet. DM digestibility was significantly higher (p<0.05) in the goats on DBG diet than in the other goats. The least DM digestibility was obtained in the goats that received CD diet (p>0.05). CP digestibility followed a similar pattern to DM digestibility. The digestibility of NDF and ADF was influenced by the nature of the diets. The digestibility of OM and GE were best (p<0.05) in the goats that received DBG, DCWM and CS byproduct diets than in CD. Significant differences (p<0.05) among the byproducts were recorded for net gas production. Potential gas production (a+b) ranged from 7.064 to 42.17 ml. Organic matter digested (OMD) from gas production value at 24 h was higher in DBG (47.6 g/kg DM) and this was followed by DCWM (42.5 g/kg DM). The least OMD was obtained in CD (17.9 g/kg DM). A significant difference (p<0.05) in DM disappearance after 4, 8, 16, 24, 48 and 72 h was recorded. The potential and effective degradability varied significantly (p<0.05) from 85.95-99.6 g/kg DM and from 39.9-65.8%, respectively. The digestibility of the byproducts in both the in vivo and in in vitro techniques demonstrated that they are potential source of feed ingredients for ruminant livestock in Samoa and possibly in the other small Pacific Island countries. On the basis of their potential degradability the byproducts could be ranked in the following order:DCWM>DBG>CD>CS. In conclusion, the results obtained suggest that all the byproducts can contribute to ruminant livestock diets without adverse effects on feed intake, growth rate and apparent nutrient digestibility coefficients.
Yahaya, M.S.;Kibon, A.;Aregheore, E.M.;Abdulrazak, S.A.;Takahashi, J.;Matsuoka, S.
Asian-Australasian Journal of Animal Sciences
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제14권4호
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pp.496-500
/
2001
In vitro and in vivo experiments were carried out to evaluate the nutritive value of three tropical browse species as assessed by DM and CP digestibility, and NDF and ADF degradability with incubation times (T) of 6, 12, 24 and 48h. During the in vivo digestibility experiment three male castrated sheep (age 16 - 25 months) with a mean liveweight of $11.5{\pm}0.9kg$ were placed in individual metabolism stalls and were allocated to one of the three browse species in a $3{\times}3$ Latin square design. The browse species were all leguminous and consisted of: Acacia sieberina (A. sieberina), Ficus polita (F. polita), and Ficus sycomorus (F. sycomorus). The mean DM and CP contents of F. polita were higher than for A. sieberina and F. sycomorus (p<0.05). In contrast the NDF and ADF contents of F. sycomorus were higher compared to the other species examined (p<0.05). The in vitro DM and CP digestibility, and NDF and ADF degradability observed at different stages of incubation were higher in F. polita followed by A. sieberina and F. sycomorus. The DM and CP digestibility at 48 h incubation were 72.92, 74.84 and 53.52% and 77.38, 77.68 and 63.64% for A. seiberina, F. polita and F. sycomorus, respectively. This shows that F. polita contains more soluble materials which ruminant can benefit from and hence has more feeding value. The fermentation of F. sycomorus was slower for all the nutrients evaluated due to the presence of more fibre. Similarly, higher in vivo digestibility coefficient of DM, CP, NDF, ADF and hemicellulose were observed for F. polita reflecting its higher values of CP, ether extract (EE) and hemicellulose associated with lower values of NDF and ADF. Higher DMI and daily gain were recorded in sheep during feeding of F. polita compared to the other species evaluated. The digestibility of all the nutrients examined were higher in the in vivo than in the in vitro trial except for CP and DM. Sheep showed no visual signs of toxicity throughout the study periods. These results showed A. seiberina, F. polita and F. sycomorus can sustain sheep on a maintenance diet and could as well be used as a supplementary feed to low producing animals during the tropical dry season. Further research is needed to ascertain the viability of using these browse species on a long-term basis.
This experiment was carried out to evaluate performance and carcass characteristics of 40 crossbred young bulls ($Zebu{\times}European$) finished in a feedlot under two roughage sources (Bermuda grass hay or sorghum silage) with or without the addition of yeast (Saccharomyces cerevisae). The bulls were 20 months old, their initial average weight was 356 kg and they were allocated into four groups of ten animals. The experimental diets were Bermuda grass, Bermuda grass+yeast, sorghum silage and sorghum silage +yeast. Animal performance and carcass characteristics were not influenced by roughage source or yeast addition. The average daily weight gain was 1.50 kg, dry matter intake (DMI) was 11.1 kg/d, DMI as percentage of liveweight was 2.60% and feed dry matter conversion was 7.70. The mean dressing percentage was 52.0% and hot carcass weight was 268 kg. Carcass conformation was classified between good-minus to good. Carcass length (137 cm), leg length (72.9 cm) and cushion thickness (26.6 cm) were not influenced by treatments. The average fat thickness was 3.80 mm and the Longissimus muscle area was 66.9 $cm^{2}$. The classification of color, texture and marbling were slightly dark red to red, fine and slight-minus to light-typical, respectively. The mean percentage of bone, muscle and fat in the carcass was 15.5%, 62.3% and 22.5%, respectively. Yeast addition increased ${\gamma}$-linolenic fatty acid (0.15 vs. 0.11%) deposition. Bermuda grass hay increased deposition of ${\alpha}$-linolenic (0.49 vs. 0.41%), arachidonic (2.30 vs. 1.57%), eicosapentaenoic (0.41 vs. 0.29%), docosapentaenoic (0.80 vs. 0.62%), docosahexaenoic (0.11 vs. 0.06%) and n-3 fatty acids, and reduced n-6: n-3 ratio in meat, when compared to sorghum silage treatments. The treatments had no effect on saturated fatty acids (49.5%), polyunsaturated fatty acids (11.8%), n-6 fatty acids (9.87%), n-3 (1.61%) and PUFA:SFA ratio (0.24). Monounsaturated fatty acid levels were higher on sorghum silage (40.7 vs. 37.7%). The addition of yeast caused higher n-6: n-3 ratio (7.28 vs. 5.70) than treatments without yeast.
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