• 식물조직배양학회지
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• 제28권5호
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• pp.239-242
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• 2001

액체배지 첨가가 L. oriental hybrid 'Casa Blanca'의 자구비대 촉진에 미치는 영향을 조사하였다. MS기본배지에 활성탄 1 g/L과 sucrose 60g/L가 첨가된 배지에서 저반부가 비대된 자구절편체 (7 mm$\times$12 mm)를 2개월간 암배양한 후에 액체배지를 동일용기에 첨가하여 자구비대를 현저하게 촉진시켰다. 첨가된 액체배지는 2배의 MS배지 염류농도에 sucrose 120g/L가 첨가된 배지 25 mL를 첨가하는 것이 자구비대에 효과적이었다.

• 식물조직배양학회지
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• 제28권4호
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• pp.185-188
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• 2001

Spathiphyllum을 기내에서 증식시킨 후, 액체배지를 첨가하여 신초의 생장 및 발근을 촉진시키고, 기외에서 순화율을 향상시켜 생산비를 감소시키기 위하여 일련의 실험을 실시하였다. 3∼4개의 신초를 가지고 있는 Spathiphyllum floribundum 'Cupid' 의 신초 cluster 절편체를 BA 2.0 mg/L가 첨가된 LS배지에서 8주간 증식시킨 후에 15 mL의 액체배지를 동일용기에 첨가하였다. 액체배지 첨가는 1/2 MS 다량요소+sucrose 50 g/L +활성탄 5.0∼10.0 g/L가 첨가된 배지 15 mL를 첨가하는 것이 식물체의 기내생장 및 발근에 양호하였으며, 온실에서 perlite와 vermiculite가 1 : 1로 혼합된 용토에 발근된 식물체를 재식하면 95% 이상 생존하였다.

• 한국약용작물학회지
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• 제20권3호
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• pp.190-194
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• 2012

Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 mg/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 mg/L BA and 0.5~1.0 mg/L IAA. Sucrose added to MS medium with 2.0 mg/L BA and 0.5 mg/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks' culture of micro-tuber sections on the medium with 2.0 mg/L BA, 0.5 mg/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 $m{\ell}$ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.

• Journal of Plant Biotechnology
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• 제39권4호
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• pp.305-308
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• 2012

장미 기내 신초 생육을 촉진시키고자 액체배지 첨가 실험을 실시하였다. 국립원예특작과학원 육성 신품종 '엔텍컬', '샤이니오렌지', '화이트젠', '레드젠' 등 4품종의 internode를 기내 도입한 후, 2회의 계대배양에 의해 신초를 증식하였다. 10~15 mL의 액체배지 첨가에 의해 4품종 모두 신초의 신장이 촉진되었고, 증식력에 있어서는 품종간 차이를 보였다.

• Journal of Plant Biotechnology
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• 제38권1호
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• pp.30-41
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• 2011

본 연구에서는 고추의 나출 소포자 배양 시 배양 중 새배지의 첨가와 진탕이 배의 생산에 미치는 영향을 조사하였다. 액체-2층 배양에서 초기 액체배양 시에 새 배지를 첨가하면 배의 발생과 발달 모두 크게 증가하였다. 가장 효과적인 첨가 시기는 초기액체 배양을 시작한 5일 후 이었다. 한편 액체-2층 배양에서 후기 2층배양 시의 새 배지 첨가는 초기 액체배양 시 첨가 때에 비해 그 효과가 적었다. 액체-2층 배양에서 후기 2층배양 시의 1주간 진탕은 정상 자엽배 생산에 효과적이었다. 액체배양시에도 배양 1주 후의 1주간 진탕은 배의 발달에 효과적 이었다. 그러나 액체-2층배양 시와 액체배양 시 모두 진탕기간이 2 ~ 3 주간으로 길어질 때에는 배의 발달이 비정상적이었다. 본 실험 결과 얻어진 정상 자엽배들은 재분화 배지에 이식 시 용이하게 유식물체로 발달하였다. 재분화 식물체들 중에는 반수체와 배가반수체가 혼재하였으며, 이들 간에는 공변세포 내 엽록체 수의 차이가 뚜렷하였다. 이와 같은 결과들은 고추에서 다수의 정상자엽배를 생산할 수 있는 소포자 배양 시스템을 확립하는 데 중요한 기초자료가 될 것이다.

• 원예과학기술지
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• 제29권5호
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• pp.494-499
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• 2011

Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun) flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing $130g{\cdot}L^{-1}$ sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing $130g{\cdot}L^{-1}$ sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment, microspore viability was maintained for 24 h at $4^{\circ}C$ without media. Among the various medium concentrations used ($0.25{\times}$, $0.5{\times}$, $1.0{\times}$, $2.0{\times}$, and $4.0{\times}$ NLN liquid medium), $0.5{\times}$ NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$, $0.5{\times}$, and $1.0{\times}$ NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$ NLN microelements, followed by incubation at $25^{\circ}C$ for 30 days.

• 생약학회지
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• 제27권4호
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• pp.301-308
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• 1996

Hairy roots induced from stems of Rubia cordifolia var. pratensis were cultured in the liquid medium under a variety of auxins to find the optimal condition for the growth and production of pigments. Culture of the hairy roots on NN liquid medium containing NAA 0.5 mg/l was best for growth of hairy roots. Production of yellow anthraquinone derivatives and purpurin in hairy roots was enhanced by the culture on NN liquid medium without auxins. Effects of L-phenylalanine, L-tyrosine and juglone, synthesized via the shikimic acid pathway, on growth and production of pigments in hairy roots were studied in the present study. Concentration of exogeneous L-phenylalanine. L-tyrosine and juglone in liquid culture system of hairy root containing NAA 0.1 mg/l was decreased quickly in its early stages of the culture period. Addition of juglone to NN liquid medium containing NAA 0.1 mg/l enhanced the productivity of pigments in hairy roots.

• Journal of Electrochemical Science and Technology
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• 제7권1호
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• pp.13-26
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• 2016

Nanoparticles (NPs), which have been investigated intensively as electrocatalysts, are usually synthesized by chemical methods that allow precise size and shape control. However, it is difficult to control the components and compositions of alloy NPs. On the other hand, the conventional physical method, sputtering with solid substrates, allows for facile composition control but size control is difficult. Recently, “liquid medium sputtering” has been suggested as an alternative method that is capable of combining the advantages of the chemical and conventional physical methods. In this review, we will discuss NP synthesis via the liquid medium sputtering technique using ionic liquid and low-volatile polymer media. In addition, potential applications of the technique, including the generation of oxygen reduction reaction electrocatalysts, will be discussed.

• 한국잠사곤충학회지
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• 제43권1호
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• pp.33-36
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• 2001

This study was carried out to improve the liquid culture methods of Paecilomyces japonica. The results show that the size of granular mycelium is smaller when the shaking speed is increased. Especially, the granular mycelium is the smallest at the shaking speed of 150rpm under the photoperiod of 12L-12D. Dry weight of mycelium was averagely 1.216 g in the Sikworm larva (SL) medium, and the weight was 2 times heavier than in the Potato dextrose (PD) medium. By adding 6 g of 6 mmbeads in the SL medium, the dry weight is increased to 1.332 g. The optimal addition of silkworm larval powder to the culture medium for gest harvest was 1.360$\pm$0.67 g in dry weight.

• Journal of Plant Biotechnology
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• 제6권4호
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• pp.241-246
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• 2004

The bulb scales and shoot sections ($7\;\cal{mm}\;\times\;15\;\cal{mm}$) of Lilium oriental hybrid 'Casablanca' were cultured to compare bulblet growth in vitro. Shoots were induced from in vitro grown bulbscales on MS medium with $1.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA, and 30 g/L sucrose. The regenerated shoots were cut into shoot sections, and cultured on MS medium with $2.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA and 30 g/L sucrose for shoot proliferation. Culture of shoot sections stimulated bulblet growth significantly than the bulb scales on MS medium with 60 g/L sucrose. However, the bulblets from shoot sections did not reach ideal size to produce stems with several leaves. Therefore, liquid medium was added into the same vessels to stimulate bulblet growth further. After shoot sections were cultured on MS medium with 60 g/L sucrose and 2 g/L activated charcoal for two months in dark, $20\;\cal{ml}$ liquid media containing various concentrations of sucrose and MS salts were added. Two months later, the added liquid medium stimulated bulblet growth remarkably as compared to bulblets grown without added liquid medium. The added $25\;\cal{ml}$ liquid medium containing 120 g/L sucrose and double strength of MS salts were the most effective for growth of in vitro bulblets. More than $94\%$ bulblets produced by this method sprouted stems with several leaves after cold treatment at $5^{\circ}C$ for three months.