• 한국식품과학회지
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• 제22권4호
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• pp.393-397
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• 1990

장려품종 콩 19종에 함유된 몇 가지 영양저해 인자의 함량과 리폭시게나아제의 역가를 조사하였다. 복부팽만인자인 라피노오스와 스타키오스의 함량은 각각 $0.74{\sim}1.58%\;3.34{\sim}5.30%$ 범위이었으며 힐, 백운, 장백 등의 품종에서 이 두 종류 당의 합량이 높았다. 콩에 함유된 트립신 저해제는 $21.2{\sim}37.0mg$ TI/g, 피트산 형태로 존재하는 인은 건물 100g 당 $337{\sim}605mg$ 정도로 품종에 따라 큰 차이를 보였다. 한편, 콩에 함유된 리폭시게나아제의 역가는 $163.6{\sim}403.5unit$로 팔달, 장백, S-133등의 품종이 높은 값을 나타내었다.

• 한국식품영양과학회지
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• 제21권1호
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• pp.23-28
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• 1992

고형상의 모델시스템에서 리놀레산의 산화에 영향을 미치는 여러가지 인자들 즉 리폭시게나아제, 수분활성도, ${\beta}$-카로틴, ${\alpha}$-토코페롤등에 대해 관찰하여 다음과 같은 결과를 얻었다. 리폭시게나아제에 의한 리놀레산의 산화반응은 aw 0.72에서 aw 0.25에 보다 잘 일어났다. 그러나 이 시스템에 ${\beta}$-카로틴이나 ${\alpha}$-토코페롤을 반응시키게 되면 리놀레산의 산화를 크게 저해하였으며 ${\beta}$-카로틴보다는 ${\alpha}$-토코페롤이 지질과 산화 과정에서 항산화작용이 더 컸다. 즉, 유사한 조건에서 ${\beta}$-카로틴은 24시간의 유도기간을 보여 준 반면 ${\alpha}$-토코페롤은 72시간까지도 유도기간이 지속되었다. ${\beta}$-카로틴의 항산화작용은 수분활성도의 영향을 크게 받았으며 수분활성도가 높을수록 항산화 작용이 뛰어났고 또한 ${\beta}$-카로틴의 농도가 높을수록 CDA 생성이 적었다.

• 생명과학회지
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• 제5권2호
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• pp.70-75
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• 1995

The effect of lipoxygenase (LOX) on the oxidation and co-oxidation of lipid fraction was studied in the model system of rainbow trout. For the reaction in model system 1 g of lipid fraction and 50mL of enzyme extract(LOX, 140 unit in 50mL phosphate buffer solution at pH 7, 4)), which were obtained from rainbow trout, were homoginized in the presence of Tween 20 and kept at 23$\circ$C for 3 days. The activity of LOX was decreased to 43% of initial level during the reaction in the model system. The initial composition of rainbow trout lipid was showed to be consisted of trigliceride(TG;82%) and free fatty acid(FFA;0.1%), while this converted to 59% of TG and 20% of FIFA, respectively after reaction in model system. Change of fatty acid composition was also observed and the content of linoleic acid, one of the major fatte acids, was decreased to 13% from 54% in the content of total fatty acids after reaction. The carotenoids in rainbow trout were composed of 0.4% $\alpha$-carotene, 1.6% $\beta$ -carotene, 80% canthaxanthin, 7% lutein and 11% zeaxanthin, thus the canthaxanthin was the major component. This canthaxanthin was the most degraded carotenoid by lipoxygenase catalyzed co-oxidation during the reaction. On the other hand the tocopherol isomers found in the rainbow trout were $\alpha$ and $\beta$ -tocopherol, and $\alpha$-tocopherol had a higher degradation rate by the lipoxygenase catalyzed co-oxidation than of $\beta$-tocopherol in the reaction of model system.

• Preventive Nutrition and Food Science
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• 제3권3호
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• pp.216-220
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• 1998

Lipoxygenase inhibitory acitivity of the methanolic extract of 60 different kinds of plant seeds was determined by a spectrophotometric method using a soybean lipoxigenase(SLO) and linolenic acid. Among the extracts examined, the methanolic extract of nutmeg(Myristical fragrans)seed showed the most potent SLO inhibitory activity. To isolate SLO inhibitor, hence, the defatted methanol extract was further partitioned with ether, ehtylacetate , and n-butanol , stepwise. The ether souble fraction was successively chromatographed on silica gel, Sephadex LH-20 and preparative TLC. Three phenolic compounds were isolated , and one of them showing a strong SLO inhibition activity was identified as a 2,6-dihydroxy-9-(3', 4', -dihydroxyphenyl)nonylphenone (IC50a=0.39$\mu\textrm{g}$/ml) by 1H-& 13C0NMR, IR, and MS spectroscopy.

• 대한화학회지
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• 제39권9호
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• pp.741-748
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• 1995

5-Lipoxyhenase의 저해제인 catechol류에 대한 구조-활성관계를 검토하였다. Catechol 유사체의 활성자리와 수용체가 작용하는 약물-수용체 상호작용 모델을 설정하여 가하학적, 전기적 파라미터를 구하여 생리활성과의 상관관계를 분석, 다중회귀분석을 실시하였다.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.447-450
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• 2002

Previously, a methanol extract from seeds of Paeonia lactiflora was shown to have a potent inhibitory activities against tyrosinase and soybean lipoxygenase (SLO). Seven stilbenes, trans-resveratrol-4-Ο-$\beta$-D-glucoside, trans resveratrol, trans-$\varepsilon$-viniferin, cis-$\varepsilon$-viniferin, gnetin H, suffruticosol A and B were isolated from the seeds as active principles for inhibition of the enzymatic activity. Among them, the resveratrol trimer, gnetin H exhibited the most potent inhibitory activities against tyrosinase and SLO, respectively. Additionally, the resveratrol dimers, trans-$\varepsilon$-viniferin and cis-$\varepsilon$-viniferin exhibited significant inhibitory activity against the two oxidative enzymes. Meanwhile, three other stilbene derivatives, such as trans-resveratrol, suffruticosol A and suffruticosol B had also weak inhibition activity. The least inhibitory activity was observed in transresveratrol-4-Ο-$\beta$-D-glucoside. These results suggest that resveratrol dimers and trimer in the seeds of Paeonia lactiflora are potentially useful therapeutic agents against pathological disorders such as hyperpigmentation and inflammation.

• 생약학회지
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• 제28권4호
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• pp.247-251
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• 1997

The effects of the extract of the root of Cynanchum wilfordi (Asclepiadaceae), the alkaloid fraction, and the isolated main constituent (gagaminine) on 5-lipoxygenase(5-LOEC 1.13.11.34) in bovine PMNL have been studied. The effect of the crude extracts of wildand cultivated plant was also compared each other. Furthermore, the inhibitory effect of gagaminine on 5-Lo was compared with those of the standard drugs. Gagaminine inhibited 5-Lo activity with an $IC_{50}$ value of $26\;{\mu}M$, this result indicates that gagaminine may be useful for in vivo experiments as 5-LO inhibitor.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1091-1095
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• 2009

The aim of this study investigated the fluctuations of 3 characters from 3 ecotypes [early ripening (ER), middle ripening (MR), and late ripening (LR)] of 20 Korean brown rice cultivars in different storage systems [time: 12 and 24 weeks, temperature: low ($10^{\circ}C$) and room ($25^{\circ}C$)]. With increase of storage time and temperature, lipoxygenase activity, and fat acidity increased, whereas germination rate was reduced. ER cultivars exhibited the highest lipoxygenase activity of $35.49{\pm}2.46$ unit/mg protein during 24 weeks storage at $25^{\circ}C$, followed by LR ($32.73{\pm}1.33$) and MR ($32.66{\pm}1.62$) cultivars. The amounts of fat acidity also were observed by the same order (ER: $20.40{\pm}2.12$>LR: $19.68{\pm}1.86$>MR: $19.64{\pm}1.35$ mg KOH/100 g). Germination rate slightly decreased with increase of time and temperature (MR>LR>ER), but MR and LR cultivars showed the most significant changes (ER: $60.90{\pm}23.47%$, MR: $32.66{\pm}13.95%$, and LR: $32.53{\pm}5.87%$). On the basis of above results, MR cultivars were evaluated the highest quality, because high lipoxygenase activity, high fat acidity, and low germination rate have deteriorated in quality and generated off-odor. Thus, MR cultivars might be very important sources in food processing and stored dietary supplement aspects.

• 산업식품공학
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• 제15권3호
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• pp.209-213
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• 2011

대두에서 산패취를 발생시키는 lipoxygenase 효소를 비가열 전처리 방법인 초고압 처리 방법을 이용하여 효소의 활성도의 변화를 확인하였다. 연구 결과 초고압 처리에 의해 lipoxygenase 효소의 활성도가 감소하는 것을 확인하였다. 또한 lipoxygenase 효소의 활성도 감소에 의해 대두의 저장성에 미치는 영향을 확인하기 위해 국산 대두와 연해주대두를 이용하여 저장 실험을 실시하였다. 실험 결과 국산대두의 경우 초고압 처리에 의해 lipoxygenase 효소의 활성도가 감소하였으나 TBA가에 영향을 주는 자동산화등의 화학반응에 의해 TBA가가 지속적으로 증가함을 보여주었다. 그러나 연해주 대두의 경우에는 초고압 처리를 한 시료의 TBA가의 증가정도가 상대적으로 낮게 측정된 것을 확인하였다. 이는 연해주 대두가 항산화 성분을 국산콩에 비하여 많이 포함하고 있어 초고압 처리에 의한 lipoxygenase 효소의 불활성과 함께 항산화 성분의 영향으로 TBA가의 저장 중 증가 정도가 낮게 측정된 것으로 판단되어진다.

• Preventive Nutrition and Food Science
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• 제4권3호
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• pp.163-166
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• 1999

Previously, the methanolic extract of Paeonia lactiflora seeds was shown to have strong ingibitory activity against soybean liposygenase (SLO). Four phenolic compounds were isolated from the seeds by solvent fractionation Sephadex LJ-20 column chromatography and preparative HPLC, and three of them showed strong SLO inhibitio and were characterized as trans-resveratrol, $\varepsilon$-viniferin and luteolin by UV, IR, 1H-NMR, 13C-NMR and MS spectrometry. trans-Resveratrol (IC50=1.02$\mu$M), $\varepsilon$-viniferin (IC50=0.81$\mu$M) and luteolin (IC50=10.01$\mu$M), first found in the above seeds, exhibited a potent SLO inhibitory activity although their activity was lower than that of a well-known lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA) (IC50=0.57$\mu$M). These results suggest that Paenia lactiflora seeds, now an unused plant seed, may be developed into useful sources of anti-inflammatory drugs.