• 한방안이비인후피부과학회지
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• 제16권1호
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• pp.112-129
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• 2003

Introduction and Objectives : Applying the saingheylyunbooemgami(SY) extract on to the atopic dermatitis(AD) is to study change of external dermal formation, change of leukocytes in vasculature, change of lipid formation in stratum corneum and distribution of ceramide and this study is done through forcing injury to rat's back skin which are lipid protect formation in stratum corneum. Materials and Methods : The AD which caused intentionally using the external application on the rat's back skin was used the SY. The change of leukocytes in vasculature has been identified through optima 5.2 and student t-test and the results were made into dermal foramtion graph. Results : After dispensing SY extract into the AD, the dermal injury was decreased, Especially, recover of lipid protection formation which include lipid and ceramide in stratum corneum is suppressing acute inflammation that some factors are PKC, TNF-${\alpha}$, lL-12B which controled the secretion of relating inflammatory cytokine, also went onto decrease of angiogenesis, and the decrese of degranulated mast cell. In addition, the decrease of epithelial injury also caused the growth of cell to decrease in stratum basale and cytoclasis. In the vasculature. the leukocytes were also decreased and it could relate to decrease AD. Conclusions : Thus. SY has effect on AD suppressing the dermal injury through recovering of lipid protection formation in stratum corneum.

• Mass Spectrometry Letters
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• 제10권1호
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• pp.11-17
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• 2019

Drosophila melanogaster (fruits fly) is a representative model system widely used in biological studies because its brain function and basic cellular processes are similar to human beings. The whole head of the fly is often used to obtain the key function in brain-related diseases like degenerative brain diseases; however the biomolecular distribution of the head may be slightly different from that of a brain. Herein, lipid profiles of the head and dissected brain samples of Drosophila were studied using electrospray ionization-mass spectrometry (ESI-MS). According to the sample types, the detection of phospholipid ions was suppressed by triacylglycerol (TAG), or the specific phospholipid signals that are absent in the mass spectrum were measured. The lipid distribution was found to be different in the wild-type and the microRNA-14 deficiency model ($miR-14{\Delta}^1$) with abnormal lipid metabolism. A few phospholipids were also profiled by comparison of the head and the brain in two fly model systems. The mass spectra showed that the phospholipid distributions in the $miR-14{\Delta}^1$ model and the wild-type were different, and principal component analysis revealed a correlation between some phospholipids (phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS)) in $miR-14{\Delta}^1$. The overall results suggested that brain-related lipids should be profiled using fly samples after dissection for more accurate analysis.

• 한국수산과학회지
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• 제17권5호
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• pp.405-413
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• 1984

전보에 이어 가물치의 지질을 체부위별(육질부, 피부. 내장부)로 유리지질과 결합지질로 분획하고, 지질 및 구성지방산의 조성을 분석, 검토한 결과를 요약하면 다음과 같다. 1. 평균적으로 유리지질은 총 지질의 $32.80\%$를 차지하여 결합지질 $3.86\%$에 비하면 약 8.5배 정도의 높은 함량이었다. 부위별로 유리지질의 함량을 보면 내장부가 $67.50\%$로서 가장 높았고 피부($20.04\%$), 육질부 ($10.85\%$)의 순이었다. 2, 유리지질중에는 중성지질이 $66.6{\sim}72.4\%$, 인지질이 $17.9{\sim}20.4\%$ 그리고 당지질이 $5.7{\sim}12.2\%$ 함유되어 있었으며, 결합지질중에는 인지질이 $28.6{\sim}50.6\%$, 중성지질이 $13.2{\sim}36.1\%$ 그리고 당지질이 $3.8{\sim}22.8\%$ 함유되어 있었다. 3. 유리지질중의 중성지질은 triglyceride(TG)가 $62.00{\sim}90.20\%$, 결합지질중의 중성지질은 esterified sterol(ES) 및 hydrocarbon(HC)이 $51.3{\sim}72.70\%$의 함량으로 가강 많았다. 한편 유리지질중의 인지질은 phosphatidyl ethanolamine(PE)이 $28.9{\sim}42.75\%$. phosphatidyl choline(PC)이 $27.85{\sim}41.06\%$로서 비슷한 함량을 보였으나, 결합지질중의 인지질은 phosphatidyl choline(PC)의 $47.18{\sim}52.54\%$, phosphatidyl ethanolamine(PE)은 $17.88{\sim}26.67\%$ 로서 많은 차이를 보였다. 4. 극성지질의 주요 구성지방산을 유리 및 결합지질로 비교하여 보면 $C_{16:0}산(21.03\%,\;22.62\%),\;C_{16:1}(8.70\%,\;30.1\%),\;C_{18:1}산(20.62\%,\;12.11\%),\;C_{22:5}(3.02\%,\;9.86\%)\;C_{22:6}산(7.56\%,\;16.02\%)$ 인 반면 비극성지질의 경우에는 $C_{16:0}산(18.98\%,\;19.12\%),\;C_{16:1}산(9.04\%,\;13.49\%),\;C_{18:1}산(22.94\%,\;11.61\%)\;C_{22:5}산(3.00\%,\;10.05\%)$였다. 5. 불포화도(TUFA/TSFA)는 결합지질이 유리지질보다 약2.5배 정도 높았으며, monoenoic acid에 대한 polyenoic acid의 비(TPEA/TMEA)르 결합지질이 유리지질보다 훨씬 높았다. 6. 필수지방산(EFA)의 함량은 유리지질이 결합지질에 비하여 높았으나 w3 고도불포화방방산(w3-HU-FA)의 함량에 있어서는 그 반대이었다. 부위별로는 지질의 함량 및 지방산의 조성이 많은 차이를 보였다.

• 한국임상약학회지
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• 제3권2호
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• pp.125-130
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• 1993

The particle size distribution and physical stability of commercial lipid emulsion inject, $intralipos^R$ before and after mixture with total parenteral nutrition(TPN) was tested. Three TNAs were prepared by adding intralipos to P-TPN, Neo-TPN and IVH-2 respectively. Particle size of fat emulsion in three TNA preparations were measured by using LPA-3000 photon correlater. Each TNAs was stored for 48 hours at $4^{\circ}C\;and\;25^{\circ}C.$ During storage, three TNAs showed the particle size in the range of 40-1000nm(about $100\%$ of total fat) and in the range of 1000-8000nm(less than $0.005\%$ of total fat). All TNAs were stable in terms of pH and visual appearance. The results showed that added lipid emulsion was stable for 48 hours at $4^{\circ}C\;and\;25^{\circ}C$.

• 동아시아식생활학회지
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• 제16권4호
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• pp.399-407
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• 2006

We evaluated the effects of butterbur (Petasites japonicus Maxim) addition to the diet on lipid profiles and antioxidant biomarkers such as total glutathionine, TBARS value, carbonyl value, GPx, GR, SOD and paraoxonase activity in the plasma or liver of mice. The distribution of body fat deposition, total cholesterol (TC) contents, and atherogenic index in the plasma were significantly decreased in the butterbur group. The levels of GSH and the activity of GR and SOD were significantly higher in the liver of the butterbur group than in that of the control group. Lipid oxidation of the liver and kidney and protein oxidation of the liver and heart were decreased in the butterbur group. Additionally, the DNA damage, as determined using the comet assay (single cell gel assay) with alkaline electrophoresis and as quantified by measuring the tail length (TL), was decreased in the butterbur group. The results of the present study showed that a diet with added butterbur exerts degenerative disease-protective effects on oxidative DNA damage and lipid peroxidation.

• Journal of Pharmaceutical Investigation
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• 제28권4호
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• pp.249-255
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• 1998

Solid Lipid Nanoparticle(SLN), one of the colloidal carrier systems, has many advantages such as good biocompatibility, low toxicity and stability. In this paper, the effects of drug lipophilicity and surfactant on the drug loading capacity, particle size and drug release profile were examined. SLNs were prepared by homogenization of melted lipid dispersed in an aqueous surfactant solution. Ketoprofen, ibuprofen and pranoprofen were used as model drugs and tweens and poloxamers were tested for the effect of surfactant. Mean particle size of prepared SLNs was ranged from 100 to 150nm. The drug loading capacity was improved with the most lipophilic drug and low concentration of surfactant. Particle size and polydispersity of SLNs were changed according to the used lipid and surfactant. The rates of drug release were controlled by the loading drug and surfactant concentration. SLN system with effective drug loading efficiency and proper particle size for the intravenous or oral formulation can be prepared by selecting optimum drug and surfactant.

• 분석과학
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• 제14권2호
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• pp.147-158
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• 2001

고분해능 가스크로마토그래프/고분해능 질량분석기를 이용하여 시화공단 지역 근로자와 일반주민의 혈액에서 다이옥신류를 분석하였다. 평균 다이옥신류의 농도는 근로자가 일반주민보다 높았다. 근로자와 일반주민의 평균 다이옥신 TEQ 농도는 각각 40.3 pg I-TEQ/g lipid, 16.6 pg I-TEQ/g lipid였고, 전체 PCBs 농도는 각각 174.1 ng/g lipid, 151.0 ng/g lipid였다. 주성분분석을 한 결과, 근로자와 일반주민의 다이옥신 이성질체 분포는 구별되었지만, 모두 식품류에서의 분포와 유사하였다. 각 개인의 일일 다이옥신 섭취량을 계산했을 때 40%만이 WHO에서 제시한 1-4 pg I-TEQs/kg bw/day의 허용범주를 만족하였다.

• 한국식품과학회지
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• 제9권4호
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• pp.264-270
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• 1977

무지방사료(無脂肪飼料) 및 여기에 17 : 0, 18 : 0 혹은 18 : 2를 첨가(添加)한 사료(飼料)를 닭병아리숫놈에 급여(給與)해서 그 경부지방조직(頸部脂肪組織) 및 천흉근(淺胸筋)의 지방산조성(脂肪酸組成)에 미치는 급여지방산(給與脂肪酸)의 영향(影響)을 조사(調査)했다. 경부지방조직(頸部脂肪組織)은 $34{\sim}62%$의 지질(脂質)을 함유(含有)해서 그 중 트리그리세라이드가 $97{\sim}98%$를 이루고 있었다. 급여(給與)한 지방산(脂肪酸)은 지방조직(脂肪組織)중에 비교적(比較的) 다량(多量) 분포(分布)하고 또는 17 : 0 급여(給與)는 16 : 1의 분포치(分布値)를 저하(低下)시키고 18 : 2 급여(給與)는 18 : 0의 분포치(分布値)를 증가(增加)시켰다. 천흉근(淺胸筋)은 $1.3{\sim}1.7%$의 지질(脂質)을 함유(含有)해서 그중 인지질(燐脂質)이 $77{\sim}79%$, 유리(遊離)콜레스테롤이 $11{\sim}13%$를 점(占)하고 있었다. 각지질분획(各脂質分劃)에서 17 : 0의 급여(給與)는 17 : 0와 17 : 1을 출현(出現)시키고 18 : 2의 급여(給與)는 18 : 2의 분포치(分布値)를 높였으나 18 : 0의 급여(給與)는 18 : 0의 분포치(分布値)에 영향(影響)을 주지 않았다. 천흉근지질(淺胸筋脂質)의 대부분(大部分)을 이루는 인지질(燐脂質)에서 18 : 0의 급여(給與)로 18 : 1이 많아졌고, 18 : 2의 급여(給與)는 18 : 1과 16 : 0의 분포치(分布値)를 저하(低下) 시키고 다시 18 : 0의 분포치(分布値)를 높였다. 또한 18 : 2의 급여(給與)는 20 : 3의 분포치(分布値)를 저하(低下) 시키고 20 : 4의 분포치(分布値)를 높였다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 1996년도 춘계학술대회 초록
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• pp.42-42
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• 1996

• 약학회지
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• 제42권3호
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• pp.265-274
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• 1998

In order to attain a sustained release at targeted organs in a prolonged time which can reduce the side effects and maximize the therapeutic effect, aclarubicin (ACL) was entrap ped into liposomes of different lipid compositions using Microfluidizer, and dry liposomes were prepared by lyophilization. The dry aclarubicin-entrapped liposomes were evaluated in terms of mean particle size and size distribution, entrapment efficiency and in vitro drug release profile. The Entrapment efficiency of liposome, when the concentration of aclarubicin and lipid were 0.5 to 1.0mg/ml and $200{\mu}mol$/ml, respectively, was over 80% using Microfluidizer, in contrast to 70% of entrapment efficiency using hand-shaking method. Mean particle size and size distribution of aclarubicin-entrapped liposomes of various lipid compositions did not change considerably by the freeze drying. The range of particle size was between 80 and 200nm. Among aclarubicin-entrapped liposomes, ACL-liposome of PC/DPPC/CH0L/TA displayed the most significant sustained release. The addition of DPPC appeared to be favorable for the control of release. In general, aclarubicin entrapped in liposomes was less stable than free aclarubicin either in pH 7.4 phosphate buffer or in human plasma. Formulation I($t_{1/2}$, 20.3 hr) devoid of lipid additive was the most unstable in the phosphate-buffer solution while formulation II($t_{1/2}$, 40.7 hr) with cardiolipin was the most stable. Half lives of aclarubicin-entrapped liposomes in human plasma were 43.2, 50.7, 35.9 and 35.3 hr for formulation I. II, III and IV, respectively, in contrast to 57.8 hr for free aclarubicin.