• 한국식품영양과학회지
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• 제20권6호
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• pp.596-602
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• 1991

옥수수 배를 benzene(BZ), n-hexane(HX), petether(PE), tricholoroethhylene(TE), chloroform-methanol(2:1, v/v) (CM), dichloromethane-methanol(2:1, v/v) (DM), hexane-diethyiether(5:1, v/v) (HD)의 7종 용매로 조지질을 추출하여 SACC법으로 비극성과 극성지질을 분리한 후 각각의 지질 조성을 TLC scanner로 측정하고 GC로 지방산 조성을 조사한 결과를 검토하였다. 조지질 추출양은 DM에서 가장 많고 다음은 CM이며 중성지질은 HX, BZ, HD에서 높은 반면 PE, DM에서 낮았으며 함량비에서는 DM과 CM에서 높으며, 당지질은 DM을 사용했을 때, CM, PE, HX 등보다 높고 HD에서 가장 낮았고 인지질은 CM에서 가장 높게 나타났다. 중성지질의 분별정량에서 triglyceride는 DM에서 높고 PM에서 낮은 편이며 1, 3-diglyceride는 CM 에서 높고 DM에서는 낮았다. 총지질의 지방산 조성은 linoleic acid가 주요 성분이며 이는 CM에서 높은 편이며 oleic acid는 PE, HD등을 사용했을 때 CM보다 높고 중성지질의 지방산 조성도 linoleic acid가 가장 많고 BZ에서 높게 나타났으며, PE, CM, HD 등에서 낮고 oleic acid는 비슷하나 DM, BZ에서는 낮다. Palmitic acid는 CM, PE에서 가장 높게 나타났다.

• Preventive Nutrition and Food Science
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• 제17권2호
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• pp.158-165
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• 2012

This study was designed to investigate whether the fatty acid composition could make a significant contribution to the oxidation stability of vegetable oils marketed in Korea. Ten kinds, 97 items of vegetable oils that were produced in either an industrialized or a traditional way were collected and analyzed for their fatty acid compositions and lipid oxidation products, in the absence or presence of oxidative stress. Peroxidability index (PI) calculations based on the fatty acid composition ranged from 7.10 to 111.87 with the lowest value found in olive oils and the highest in perilla oils. In the absence of induced oxidative stress, malondialdehyde (MDA), the secondary lipid oxidation product, was generated more in the oils with higher PI (r=0.890), while the tendency was not observed when the oils were subjected to an oxidation-accelerating system. In the presence of the oxidative stress, the perilla oils produced in an industrialized manner generated appreciably higher amounts of MDA than those produced in a traditional way, although both types of oils presented similar PIs. The results implicate that the fatty acid compositions could be a predictor for the oxidation stability of the vegetable oils at the early stage of oil oxidation, but not for those at a later stage of oxidation.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1253-1261
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• 1998

Effects of dietary cholesterol and taurine supplementation on hepatic total and phospholipid fatty acid compositions were evaluated in rats fed one of the following semisynthetic diets for 5 weeks : control diet(CD, cholesterol free and taurine free diet); high cholesterol diet(HCD, CD＋1.5% cho lesterol); high cholesterol, high taurine diet(HCHTD, HCD＋1.5% taurine). Diet induced changes in hepatic total fatty acid compositions were very similar to those in hepatic phospholipid fatty acid compositions. The HCD significantly decreased the percentage of total saturated fatty acids(SFA), and increased the percentage of total monounsaturated fatty acids(MUFA) of hepatic total lipids and phospholipids as compared to the values for the control rats(p<0.001). HCHTD significantly elevated the percentage of $\Sigma$SFA and lowered the percentage of $\Sigma$MUFA compared to the values for the HCD(p<0.001). Percentages of hepatic total and phospholipid 18:3$\omega$3, 20:5$\omega$3, 18:2$\omega$6 and 20:3$\omega$6 were significantly higher in rats fed the HCD than the values for the control rats, and the percentages of their elongation and desaturation products(22:5$\omega$3, 22:6$\omega$3, 20:4$\omega$6, 22l:4$\omega$6 and 22: 5$\omega$6) were significantly lower in rats fed the HCD compared to those for the control rats. HCD significantly lowered the Δ5 desaturation(20:3$\omega$6⇒20:4$\omega$6) and Δ4 desaturation(22:4$\omega$6⇒22:5$\omega$6) indices, and the elongation index of $\omega$3 fatty acid(20:5 $\omega$3⇒22:5$\omega$3) in rat liver. HCHTD reversed the cholesterol induced changes in the compositions of $\omega$3 and $\omega$6 fatty acids. These results suggest the possibility that dietary cholesterol and taurine supplementations affect plasma and liver lipid levels, at least in part, by changing the hepatic phospholipid fatty acid compositions and thereby modulating the physical characteristics of the membrane and the activities of microsomal enzymes involved in lipid metabolism.

• 한국식품영양과학회지
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• 제27권3호
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• pp.376-380
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• 1998

The chemical copositions in rice hulls of twenty six varieties which are one of the largest agricultural by-products in Korea were analyzed. The chemical compositions were 8.56~9.70% moisture, 10.01~17.16% ash, 44.02~55.50% crude fiber, 0.26~2.90% crude protein, 9.20~14.50% starch, and 0.28~0.69% lipid. There were no significantly difference in chemical compositions among twenty six varities. The mineral contents(mg/100g) were 14.3~392.4mg K, 59.4~389.1mg Ca, 31.78~377.8mg Zn, 19.4~104.4mg Na, 12.9~47.6mg Mg, 12.8~37.0mg Si, 8.3~30.5mg Fe, 7.9~26.1mg Al, 7.6~23.5mg Mn, and 6.3~40.3mg P. The contents of IDF (insoluble detergent fiber), SDF(soluble detergent were 70%, 1~2%, 67~73%, 51~60%, 11~17% and 0.3~2%, respectively. The lignin content was higher in Hwa-Young and Mankum than in others. The amounts of ethanol extracts of rice hulls were 1.01~1.52%. In conclusion, the chemical compositions were not significantly difference among twenty six rice hulls varieties.

• 한국식품영양과학회지
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• 제20권1호
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• pp.59-64
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• 1991

Studies comparing the relative effects of water soluble extract of Ganoderma lucidum kale juice and sodium dextrothyroxine on lipid component in liver brain and testis of hypercholeste-rolemic rats for 4 weeks. Therefore the aim of this work was to investigate for prevention in cardiovascular disease, Total cholesterol concentration in liver were significantly lower kale juice and sodium dextrothyroxine(1.25mg/kg diet) fed groups than the other groups and trigly-ceride concentration was significantly lower Ganodrma lucidum fed group than in control group;. Total cholesterol concentration in testis were lower other groups than in control group and triglyceride concentration were significantly lower cholesterol Ganoderma lucidum and kale juice groups in control group. Phospholipid concentration in testis was higher sodium dextroth-yroxine fed group thanin control group.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1979년도 추계학술대회 심포지움
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• pp.244.1-244
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• 1979

An attempt was made to produce by using Rh. gracilis (NRRL Y-1091) in the same wav as currently considered siglecell protein production. The lipid content of Rh. gracilis were 56.9％ of dry weight. Economic coefficient (E. C.) and fat coefficient (F. C.) were 31.3 and 17.8, respectivelv. The lipid and fats extracted from Rh. gracilis were characterized as following: Specific gravity 0.875, A.V. 42.5, S.V. 220, I.V. 57.7, Phospholipid 1.58, Unsaponifiables 25.5, Volatile matter 18.7. Fatty acid composition of the lipids were analvzed by HPLC. In addition to the lipid analvsis, proteins were extracted from defatted Rh. gracilis and their amino acid compositions were examined by amino acid analyzer.

• Journal of Plant Biology
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• 제31권2호
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• pp.83-89
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• 1988

The Fucales plants have very homogenous taxonomic characteristics at order level, but show many morphological variatations at species or subspecies levels. This study is one of the serial works to obtain more taxonomic data than morphotaxonomic ones and to clarify the taxonomic ranks and characteristics of Korean Fucales plants through physiological and biochemical analyses. Total lipid compositions of Korean Fucales plants were analyzed by the 3-dimensional TLC method. Major components of phospholipids (PA, PC, PG, PE, DPG, LPE, LPC), neutral fat TG and sterols were commonly contained in each species. Unknown lipids X1, X2 and X3 were contained in genus Sargassum, but Hizikia was lacking X3 and Myagropsis was lacking both X2 and X3. The latter contained unique X8. From the view-point of the phylogeny of lipid metabolism, Sargassum might be more closely related to Hizikia than to Myagropsis. A variant of S. patens from Seongsan showed minute metabolic difference from that of the typical plant; but a variant of S. serratifolium did not show any significant difference from that of the typical plant.

• 한국식생활문화학회지
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• 제5권4호
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• pp.437-442
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• 1990

국내에서 생산되는 재배버섯으로 일반느타리(Pleurotus ostreatus)와 고온성느타리(Pleurotus sajor-caju) 및 야생식용버섯으로 싸리버섯(Ramaria botrytis)과 젖버섯(Lactarius volemus)의 지질성분조성을 비교 검토한 결과 각 버섯의 지방함량은 $0.32{\sim}0.39%$이었으며, 그 조성은 인지질$(19.4{\sim}47.4%)$, 중성지질$(32.1{\sim}51.9%)$, 당지질$(14.8{\sim}28.7%)$ 순으로 많았으나 고온성 느타리는 다른 버섯에 비하여 중성지질(51.9%)의 함량이 높았다. 중성지질에서는 triglyceride$(21.2{\sim}38.2%)$, free sterol$(21.0{\sim}21.9%)$, sterol ester$(10.3{\sim}18.6%)$ 순으로 많았으나 고온성 느타리는 free fatty acid(34.1%)의 함량이 다른 버섯에 비하여 특이하게 높았다. 당지질은 steryl glycoside$(15.6{\sim}29.4%)$, esterified steryl glycoside$(13.4{\sim}23.9%)$, monogalactosyl diglyceride$(15.6{\sim}24.6%)$ 순으로 많았으며, 인지질의 조성은 phosphatidyl choline$(36.7{\sim}49.5%)$, phosphatidyl ethanolamine$(20.9{\sim}29.7%)$, phosphatidyl inositol$(18.4{\sim}26.1%)$ 순으로 많았다. 각 구성지질의 지방산조성은 4종의 버섯 공히 linoleic acid와 palmitic acid가 주요 지방산이었으나 싸리버섯은 중성지질에서 oleic acid의 함량이 높았다.

• Applied Biological Chemistry
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• 제37권6호
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• pp.509-515
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• 1994

한국산 마의 주요 품종인 Dioscorea batatas, D. aimadoimo and D. japonica 3가지 품종의 시료들을 동결건조하여 분말로 만든 다음 지질의 추출, silicic acid column chromatography에 의한 지질의 분획, thin layer chromatography에 의한 지질의 분리 및 gas chromatography에 의한 지방산 조성 등을 행하였다. 총지질의 함량은 D. japonica가 다른 마에 비해 적은 편이었으며, 지질을 분획한 결과 3가지 품종 모두 중성지질이 가장 많았고 그 다음 당지질, 인지질 순으로 나타났다. 분획한 각 지방의 구성 지질로는 중성지질이 sterolester, triglyceride, 1,3-diglyceride, 1,2-diglyceride 및 monoglycerid였고, 당지질에서 acylsterylglycoside, sterylglycoside, digalactosyldiglyceride 및 sulfolipid였고, 인지질에서 phosphatidylethanolamine, phosphatidylcholine 및 phosphatidylinositol 이었다. GC에 의한 총지질의 지방산 조성은 팔미트산과 리놀레산이 가장 많은 부분을 차지하였고 불포화지방산 함량이 포화지방산 함량보다 많았다. 또한 중성지질, 당지질 및 인지질의 지방산 조성도 총 지질을 구성하는 지방산 조성과 같은 양상을 보여 주었다.

• 한국응용곤충학회지
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• 제55권2호
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• pp.129-138
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• 2016

아이코사노이드는 탄소수 20 개의 다가불포화지방산 산화물로 구성된다. 이들 다가불포화지방산의 생합성 전구물질을 탐지하기 위해 파밤나방(Spodoptera exigua)의 서로 다른 조직으로부터 지방산을 분리하여 GC/MS로 조성을 분석하였다. 파밤나방 5령 유충에서 소화관, 지방체, 혈구 및 체벽을 분리하고, 각 조직에서 지질을 추출하여 각각 중성지질, 당지질 및 인지질로 분리하였다. 대부분의 조직은 palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2) 그리고 linolenic acid (18:3)를 주요 지방산으로 함유하였다. 그러나 이들 지방산의 조성은 조직과 지질 종류에 따라 상이하였다. 지방체와 혈구세포는 이들 주요 지방산 이외에 myristic acid (14:0)와 3 종류의 미동정 지방산들이 추가로 검출되었다. 서로 다른 지질 종류 가운데 인지질은 중성지질이나 당지질에 비해 상대적으로 높은 linolenic acid를 지닌 반면 포화지방산의 함유량은 낮았다. 전체 불포화지방산의 조성도 조직과 지질 종류에 따라 상이하였다. 인지질은 지방체, 혈구 및 소화관에서 높은 불포화지방산 함유량을 나타냈다. 세포성 인지질분해효소인 calcium-independent phospholipase $A_2$ ($iPLA_2$)는 지방산 조성을 조절하는 데 역할을 담당하였다. 이 유전자의 RNA 간섭은 중성지질과 인지질에서 지방산 조성의 변화를 유발하였다. 본 연구는 아이코사노이드 생합성의 전구물질로 여겨지는 아라키도닉산을 검출하지 못했다. 이는 곤충에 있어서 아이코사노이드는 포유동물과는 다른 새로운 생합성 과정을 통해 형성되는 것으로 추정된다.