• 한국수산과학회지
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• 제18권5호
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• pp.439-446
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• 1985

양식 뱀장어의 품질을 지질성분면에서 평가하기위하여 천연 및 양식 뱀장어의 지질 조성 및 이들 지질의 구성지방산을 분석${\cdot}$비교하였고, 붕장어의 지질성분과도 비교하였다. 또 양식 뱀장어의 지방산과 사료 지방산을 분석${\cdot}$비교한 결과는 다음과 같다. 1. 총지질 함량은 양식 뱀장어가 천연 뱀장어 보다는 약간 높은 반면 붕장어 보다 약 2배 정도 높았다. 부위별 지질 함량은 뱀장어는 가식부($27.16{\sim}29.70\%$)가 내장부($5.10{\wim}5.86\%$)보다 훨씬 높았으나 붕장어는 거의 비슷하였다. 2. 천연 및 양식 뱀장어와 붕장어의 지질 조성은 거의 비슷한 경향을 나타내고 있었다. 즉 중성지질이 가장 많고, 인지질, 당지질의 순이었다. 또 부위별 지질 조성은 중성지질은 가식부가 내장부보다. 많은 반면 인지질과 당지질은 내장부가 가식부보다 많았다. 또 중성지질은 TG의 함량이 가장 많았으며 인지질은 PC와 PE가 가장 많았다. 3. 식용으로 이용되는 가식부의 중성지질은 $C_{14:0},\;C_{16:0},\;C_{18:1}$산은 양식 뱀장어가 천연 뱀장어보다 높은 반면 $C_{16:1},\;C_{18:2},\;C_{18:3},\;C_{20:4},\;C_{20:5},\;C_{22:5},\;C_{22:6}$ 산은 천연산이 양식산보다 높았다. 붕장어의 경우는 $C_{18:0},\;C_{20:4},\;C_{22:6}$산이 천연 및 양식 뱀장어에 비해 훨씬 높은 것이 특징적이었다. 또 인지질의 경우는 $C_{18:0},\;C_{18:2}$산이 양식 뱀장어가 천연 뱀장어보다 높은 반면 $C_{16:0},\;C_{16:1},\;C_{18:1},\;C_{18:3},\;C_{20:4},\;C_{20:5},\;C_{22:5},\;C_{22:6}$산은 천연산이 양식산보다 높았다. 4. 중성지질의 불포화도(TUFA/TSFA)는 천연 및 양식 뱀장어와 붕장어 사이에는 차이가 거의 없었지만, 인지질의 경우는 천연 뱀장어가 양식 뱀장어 및붕장어 보다 높았다. 가식부의 필수지방산 함량은 중성지질은 천연산이 양식산보다 3배이상 높았으며, 또 붕장어는 뱀장어보다 훨씬 높았다. 그러나 인지질의 경우에는 필수지방산 함량에 큰 차이가 없었다. 5. 가식부의 W3 고도불포화지방산 함량은 중성지질은 천연 뱀장어가 양식 뱀장어 및 붕장어보다 $2.0{\sim}2.5$배 정도 높았으며 인지질의 경우도 천연 뱀장어가 양식뱀장어 및 붕장어보다 훨씬 높았다. 6. 사료 지방산(B)에 대한 양식 뱀장어의 지방산 (A)의 비(A/B)를 비교하여 보면 linoleic acid ($C_{18:2}\;w6$), linolenic acid ($C_{18:3}\;w3$) 등의 필수지방산과 eicosapentaenoic acid ($C_{20:5}\;w3$), docosahexaenoic acid ($C_{22:6}\;w3$)등의 w3 고도불포화지방산의 A/B비가 $0.23{\sim}0.48$로 아주 낮아, 이들 불포화지방산의 polyene 산의 생합성 및 체성장에 크게 관계할 것으로 판단되었다.

• Preventive Nutrition and Food Science
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• 제6권3호
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• pp.176-179
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• 2001

The present study was designed to investigate the hypoglycemic activity and lipid metabolism of ethanol (EtOH) extract from the aleurone layer of anthocyanin-pigmented (AP) rice in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 210~240 g were divided into 4 groups, normal, diabetic control, and two experimental groups, and diabetes in rats was induced by injection of STZ (45 mg/kg, body weight) into tail vein. The EtOH extract of the powdered aleurone layer of AP rice was administered orally in diabetic rats for 14 days. In order to find the hypoglycemic effects in the animal model, the body weight, plasma glucose levels, cholesterol, HDL-cholesterol, triglyceride (TG), free fatty acid (FFA), aspartate aminotransferase (AST) and alanine amino- transferase activities (ALT) were determined. Oral administration of 1.0 81kg on the EtOH extract for 14 days resulted in a significant reduction in blood glucose, ALT, TG and FFA. However, in the case of 2.0 g/kg, the hypo-glycemic effects were not considerable. This results suggest that the EtOH extract might induce hypoglycemic effects in STZ-induced diabetic rats due to some photochemical components in the aleurone layer of AP rice.

• 한국환경과학회지
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• 제21권7호
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• pp.875-881
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• 2012

This study was performed to observe the effects of the feeding physiological activity substance in Cordyceps militaris hot-water extract intake on the improvement of lipid components and metabolic enzyme activities in the serum of dietary hyperlipidemic rats (SD strain, male) fed the experimental diets for 5 weeks. The concentrations of cholesterol (total cholesterol, ${\beta}$-lipoprotein, LDL-cholesterol, free cholesterol and cholesteryl ester), atherosclerotic index, triglyceride (TG), phospholipid (PL) and blood glucose in sera were remarkably lower in the Cordyceps militaris extract intake group than in the hyperlipidemic rats. In the ratio of HDL-cholesterol concentration was higher percentage in the Cordyceps militaris extract intake group than in the hyperlipidemic rats. The activities aminotransferase (AST, ALT) in sera were rather lower in the Cordyceps militaris extract administration than in the hyperlipidemic rats. From the above research, Cordyceps militaris extract were effective on the improvement of the lipid compositions in sera of dietary hyperlipidemic rats.

• BMB Reports
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• 제35권5호
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• pp.459-464
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• 2002

Fusogenic liposomes that incorporate Sendai virus envelope proteins, so-called Sendai virosomes, have been developed for in vitro and in vivo genetic modification of animal cells. In this study, several different virosomes of varying lipid compositions were formulated and their in vitro gene-transfer efficiencies compared. The virosomes were prepared by quantitative reconstitution of the Sendai envelope, fusion (F) and hemagglutinin-neuraminidase (HN) proteins into liposomal vesicles. Virosomes that contained luciferase reporter genes were tested in 293 transformed human kidney cells. F/HN-virosomes that were prepared with an artificial Sendai viral envelope (ASVE-virosomes) or phosphatidylserine (PS-virosomes) exhibited an 8- or 6-fold higher gene-transfer efficiency than cationic liposomes that were made with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). F/HN-virosomes that were prepared with phosphatidic acid (PA-virosomes) instead of PS were less efficient in gene transfer than either ASVE- or PS-virosomes. In addition, the genetransfer capability of ASVE- and PS-virosomes was maximal at a $Ca^{2+}$ concentration of 510 mM. These results suggest that the incorporated lipid components significantly affect the in vitro gene transfer that is mediated by Sendai F/HN-virosomes.

• 생약학회지
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• 제25권3호
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• pp.226-232
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• 1994

An antioxidant activity of Gardenia Fruit (Gardenia jasminoides Ellis) which has been used for food coloring was studied. The antioxidant activity was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). Both water and methanol extracts of Gardenia Fruit showed the antioxidant activity. On solvent fractionation, the antioxidant activity was removed into the ethyl acetate and butanol soluble fractions. And the final water soluble fraction also showed the antioxidant activity in the low concentration, but it promoted the lipid peroxidation in the high concentration. Two compounds (I and II) having the antioxidant activity were isolated from the butanol fraction, and compound I also occurred in the ethyl acetate fraction. The antioxidant activity of compound II was more potent than that of I. By analyzing data for UV, IR and $^1H-NMR$, compounds I and II were identified as geniposide and crocin, respectively.

• Journal of Nutrition and Health
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• 제24권2호
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• pp.77-86
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• 1991

Changes in total lipid content. total cholesterol content and fatty acid composition of human milk were investigated longitudinaly from 2-5 days to 12 weeks postpartum Milk samples were collected from 19 Korean lactating women at 2-5 days and at 1. 2. 4. 6. and 12 weeks postpartum. The obtained results were as follows : On average the daily energy intake protein intake and fat intake of Korean lactating women was 1812 kcal 72.5g and 29.8g respectively. The composition ratio of energy consis-ting of protein fat and carbohydrate was 16:15:69 The total lipid content increased from 1.39g/이 at 2-5 days to 2.86g/dl at 12 weeks ; while the total cholesterol concentration (mg/g) decreased significantly with time following postpartum. The total unsaturated fatty acids content was higher in colostrum than in mature milk. and the total saturated fatty acids were higher in mature milk. The average DHA content was 0.55% and the P/S ratio of human milk lipids was 0.37.

• Nutritional Sciences
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• 제6권1호
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• pp.12-19
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• 2003

The measurement of the total antioxidant capacity (TAC) of human plasma has been widely applied in nutritional science, for example to evaluate the antioxidant contribution of dietary components and to study, although indirectly, the bioavailability of dietary antioxidants. Several methods have been proposed for the measurement of TAC, most of them based on the ability of plasma to withstand the oxidative damage induced by aqueous radicals. Although plasma contains both hydrophilic and lipophilic antioxidants that interact through extensive cross-talk in most of the methods employed for the TAC measurement, the hydrophilic antioxidants such as ascorbic acid, uric acid, and protein thiols mainly contribute to the total antioxidant plasma capacity (almost 70%) while lipophilic antioxidants embedded in the lipoproteins (carotenoids, a-tocopherol, ubiquino1-10) participate only in a negligible amount (less than 5%). The present paper reviews the analytical methods used to assess the TAC and in particular focuses on new approaches that are capable of distinguishing the antioxidant capacity of both the aqueous and lipid compartments of plasma. The general principle of the method as well as some in vitro and ex vivo applications will be discussed within the text.

• 대한수의학회지
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• 제17권2호
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• pp.41-49
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• 1977

The present study was underve the histochemical nature of various follicles and interstitial tissues in the ovaries of Korean native goats and cattle as well as the histochemical changes of those in the ovaries of Korean native goats treated with dexamethasone. Much more lipid granules appeared in the granulosa and theca cells of atretic follicles compared with normal follicles in these ruminant ovaries. In the ovaries of Korean native goats the interstitial tissue derived from the theca interna of atretic follicles appeared the form of patches of cells and the interstitial tissue derived from stromal cells appeared the form of diffuse, obscure bounds. In the ovaries of Korean native cattle the interstitial tissue derived from theca interna of atretic follicles showed sparsely scattered form of pathes of cells. The histochemical components were described on the basis of lipids in the granulosa and theca cells of normal follicles, atretic follicles and interstitial tissue. In the ovaries of Korean natve goats treated with dexamethasone, the granulosa and theca cells of atretic foillicle contained plenty lipid granules that were increased in size and number, however, lipid granules were markedly decreased in the interstitial tissue.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.61-61
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• 2003

The ethanol extract of peony root (Paeonia Lactiflora Pall, Paeoniaceae) and its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. And protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were performed. The ethanol extract of peony root (PRE), gallic acid and methyl gallate were shown to possess the significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and were revealed the inhibitory effect of lipid peroxidation as expressed by malondialdehyde (MDA) formation. They were also found to strongly inhibit hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, assessed by single cell gel electrophoresis. Furthermore, oral administration of 50% PRE (50% ethanol extract), gallic acid and methyl gallate potently inhibited micronucleated reticulocyte (MNRET) formation of mouse peripheral blood induced by KBrO3 treatment in vivo. Therefore, PRE containing gallic acid and methyl gallate may be a useful natural antioxidant by scavenging free radicals, inhibition of lipid peroxidation and protecting oxidative DNA damage.

• Journal of Forest and Environmental Science
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• 제32권2호
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• pp.103-112
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• 2016

To compare seed components of plus trees, seed powder ground after seedcoat removal was analyzed for two oak species, i. e., Quercus monglica (white oak) and Quercus variabilis (red oak), which are typical oak trees in Korea but have different fruiting characteristics. Thus we aimed at analyzing and comparing many ingredients including minerals, sugars, etc. Two species were similar to each other in the content of water, crude ash, crude protein and carbohydrates, but crude lipid content in Q. variabilis was 2.5 times higher than that in Q. mongolica. Crude proteins of Clone 124 was 1.5 times higher than that of Clone 75 in Q. mongolica. Crude lipid content showed the highest value in Clone 0511 of Q. variabilis, and more phosphate and iron was found in Q. monglica than in Q. variabilis. Glucose showed 85.4% and 88.3% on average of the total monosacchrides in two species, and galactose and arabinose were also found. In the content of phosphate, iron, and crude lipid, differences were found between two species and among clones of two species.