BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Lipid Compositions on Gene Transfer into 293 Cells Using Sendai F/HN-virosomes

  • Kim, Hong-Sung (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Park, Yong-Serk (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University)
  • Published : 2002.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Fusogenic liposomes that incorporate Sendai virus envelope proteins, so-called Sendai virosomes, have been developed for in vitro and in vivo genetic modification of animal cells. In this study, several different virosomes of varying lipid compositions were formulated and their in vitro gene-transfer efficiencies compared. The virosomes were prepared by quantitative reconstitution of the Sendai envelope, fusion (F) and hemagglutinin-neuraminidase (HN) proteins into liposomal vesicles. Virosomes that contained luciferase reporter genes were tested in 293 transformed human kidney cells. F/HN-virosomes that were prepared with an artificial Sendai viral envelope (ASVE-virosomes) or phosphatidylserine (PS-virosomes) exhibited an 8- or 6-fold higher gene-transfer efficiency than cationic liposomes that were made with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). F/HN-virosomes that were prepared with phosphatidic acid (PA-virosomes) instead of PS were less efficient in gene transfer than either ASVE- or PS-virosomes. In addition, the genetransfer capability of ASVE- and PS-virosomes was maximal at a $Ca^{2+}$ concentration of 510 mM. These results suggest that the incorporated lipid components significantly affect the in vitro gene transfer that is mediated by Sendai F/HN-virosomes.

Keywords

References

  1. AI-Ahdal, M. N., Abidi, T. F. and Fianagan, T. D. (1986) The interaction of Sendai virus glycoprotein-bearing recombinant vesicles with cell surface. Biochem. Biophys. Acta 854, 157-168. https://doi.org/10.1016/0005-2736(86)90107-0
  2. Aliza. Y. and Abraham, L. (1972) The mechanism of cell fusion. J. Biol. Chem. 247, 4021-4028.
  3. Aloria, R. C., Jensen, F. C., Curtin, C. C., Moblet, P. W. and Gordon, L. M. (1988) Lipid composition and fluidity of the human immunodeficiency virus. Proc. Natl. Acad. Sci. USA 85, 900-904. https://doi.org/10.1073/pnas.85.3.900
  4. Bagai, S., Puri, A., Blumenthal, R. and Sarkar, D. P. (1993) Hemagglutinin-neuraminidase enhances F protein-mediated membrane fusion of reconstituted Sendai virus envelope with cells. J. Virol. 67, 3312-3318
  5. Bagai. S. and Sarkar, D. P. (1994) Fusion-mediated microinjection of lysozyme into HepG2 cells through hemagglutinin neuraminidase-depleted Sendai virus envelopes. J. BioI. Chem. 369, 1966-1972.
  6. Citovsky, V., Yanai, P and Loyter, A (1986) The use of circular dichroism to study conformational changes induced in Sendai virus envelope glycoproteins. A correlation with the fusogenic activity. J. BioI. Chem. 261, 2235-2239.
  7. Choi. J. S., Lee, E. J., long, H. S. and Park. J. S. (2000) 3b[L-Lysinamide-Carbamoyl] Cationic lipid as a biocompatible efficient gene transfer. J. Biochem. Mol. BioI. 33, 476-482.
  8. Dzau, V. J., Mann. M. J., Morishita, R. and Kaneda, Y (1996) Fusogenic viral liposome for gene therapy in cardiovascular disease. Proc. Natl. Acad. Sci. USA 93. 11421-11425. https://doi.org/10.1073/pnas.93.21.11421
  9. Gitman, A G., Graessmann, A. and Loyter, A. (1985) Targeting of loaded Sendai virus envelopes by covalently attached insulin molecules to virus receptor-depleted cells: Fusion-mediated microinjection of ricin $\AA$ and simian virus 40 DNA Proc. Natl. Acad. Sci. USA 82, 7309-7313. https://doi.org/10.1073/pnas.82.21.7309
  10. Hirano, T., Fujimoto. J., Ueki, T., Yamamoto, H., Iwasaki, T., Morisita, R., Sawa, Y., Kaneda, Y., Takahashi, H. and Okamoto, E. (1998) Persistent gene expression in rat liver in vivo by repetitive transfections using HVJ-Iiposome. Gene Ther. 5, 459-464. https://doi.org/10.1038/sj.gt.3300617
  11. Hsu, M. C., Schid, A. and Choppin, P. W. (1982) Enhancement of membrane-fusing activity of Sendai virus by exposure of the virus to basic pH is correlated with a conformational change in the fusion protein. Proc. Natl. Acad. Sci. USA 79. 5862-5866. https://doi.org/10.1073/pnas.79.19.5862
  12. Kaneda, Y., Saeki, Y. and Morishita, R. (1999) Gene therapy using HVJ-liposomes: the best of both worlds? Mol. Med. Today 5. 298-303. https://doi.org/10.1016/S1357-4310(99)01482-3
  13. Kim, H. S. and Park, Y. S. (2002) Gene transfection by quantitatively reconstituted Sendai envelope proteins into liposomes. Cancer Gene Therapy 9, 173-177. https://doi.org/10.1038/sj.cgt.7700421
  14. Kumar, M. and Sarkar, D. P (1996) F protein induced fusion of Sendai viral envelopes with mouse tetra carcinoma cells through $L_{ex}$-$L_{ex}$ interaction. FEBS Lett. 391, 17-20. https://doi.org/10.1016/0014-5793(96)00698-9
  15. Kumar, M., Hassan, Q., Tygaii, S. K. and Sarkar, D. P. (1997) A 45,000-Mr glycoprotein in the Sendai virus envelope triggers virus-cell fusion. J. Virol. 71. 6398-6406.
  16. Jendrasiak, G. L., Smith. R. L. and Shaw, W. (1996) The water adsorption characteristics of charged phospholipids. Biochim. Biophys. Acta 1279, 63-69. https://doi.org/10.1016/0005-2736(95)00244-8
  17. Nakanishi, M., Uchida, T., Kim, J. and Okada, Y. (1982) Glycoproteins of Sendai virus (HVJ) have a critical ratio for fusion between virus envelopes and cell membranes. Exp. Cell. Res. 142, 95-101. https://doi.org/10.1016/0014-4827(82)90413-X
  18. Ponimaskin. E., Bareesel, K. K. H., Markgraf, K., Reszka, R., Lehmann, K., Gelderblom, H. R., Gawaz. M. and Schmidt, M. F. G. (2000) Sendai virosomes revised: Reconstitution with exogenous lipids leads to potent vehicles for gene transfer. Virology 269, 391-403. https://doi.org/10.1006/viro.2000.0233
  19. Sechoy. O., Philippot. J. R. and Bienvenue, A (1986) Preparation and characterization of F protein vesicles isolated from Sendai virus by means of octylglucoside. Biochem. Biophys. Acta 857, 1-12. https://doi.org/10.1016/0005-2736(86)90093-3
  20. Tomasi, M. and Loyter, A (1981) Selective extraction of biologically active F-glycoprotein from dithiothreitol reduced Sendai virus particles. FEBS Lett. 131, 381-385. https://doi.org/10.1016/0014-5793(81)80409-7
  21. Tomita. M., Morishita, R., Higaki, J., Tomita, S., Aoki, M., Ogihara. T. and Kaneda, Y. (1996) In vivo gene transfer of insulin gene into neonatal rats by the HVJ-Iiposome method resulted in sustained transgene expression. Gene Ther. 3, 477-482.
  22. Vainstein, A., Hershkovitz, M., Israel, S., Rabin, S. and Loyter, A. (1984). A new method for reconstitution of highly fusogenic Sendai virus envelopes. Biochem. Biophys. Acta 773, 184-188.
  23. Yang. Q., Guo, Y., Li, L. and Hui, S. W. (1997) Effects of lipid headgroup and packing stress on poly(ethylene glycol)-induced phospholipid vesicle aggregation and fusion. Biophys. J. 73, 277-282. https://doi.org/10.1016/S0006-3495(97)78068-8

Cited by

  1. Identification of the Most Accessible Sites to Ribozymes on the Hepatitis C Virus Internal Ribosome Entry Site vol.36, pp.6, 2003, https://doi.org/10.5483/BMBRep.2003.36.6.538
  2. Liposomes and virosomes as delivery systems for antigens, nucleic acids and drugs vol.15, pp.6, 2004, https://doi.org/10.1016/j.copbio.2004.10.005