• Journal of Microbiology and Biotechnology
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• 제32권5호
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• pp.621-629
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• 2022

Bacterial outer membrane vesicles (OMVs) typically contain multiple immunogenic molecules that include antigenic proteins, making them good candidates for vaccine development. In animal models, vaccination with OMVs has been shown to confer protective immune responses against many bacterial diseases. It is possible to genetically introduce heterologous protein antigens to the bacterial host that can then be produced and relocated to reside within the OMVs by means of the host secretion mechanisms. Accordingly, in this study we sought to develop a novel platform for recombinant OMV (rOMV) production in the widely used bacterial expression host species, Escherichia coli. Three different lipoprotein signal peptides including their Lol signals and tether sequences-from Neisseria meningitidis fHbp, Leptospira interrogans LipL32, and Campylobactor jejuni JlpA-were combined upstream to the GFPmut2 model protein, resulting in three recombinant plasmids. Pilot expression studies showed that the fusion between fHbp and GFPmut2 was the only promising construct; therefore, we used this construct for large-scale expression. After inducing recombinant protein expression, the nanovesicles were harvested from cell-free culture media by ultrafiltration and ultracentrifugation. Transmission electron microscopy demonstrated that the obtained rOMVs were closed, circular single-membrane particles, 20-200 nm in size. Western blotting confirmed the presence of GFPmut2 in the isolated vesicles. Collectively, although this is a non-optimized, proof-of-concept study, it demonstrates the feasibility of this platform in directing target proteins into the vesicles for OMV-based vaccine development.

• 한국수자원학회논문집
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• 제37권5호
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• pp.387-395
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• 2004

본 논문에서는 하상변동 및 유사입자의 시간적 공간적 분포를 동시에 모의할 수 있는 2차원 수치모형을 제시하였다. 사행수로의 하상변동은 만곡부의 외측에서는 하상이 세굴되고 훨씬 더 거친 입경의 재료로 구성되며, 만곡부의 내측에서는 하상이 상승하고, 세립토로 구성되었다. 또한 사행하천에서 흐름의 방향에 대한 입자분포 과정은 상류의 변곡점에서 시작하여, 하류 변곡점에서 마치게 되는 것을 보여주었다. 하천의 경계가 불규칙하고 복잡한 하천에서는 혼합사호 형성된 하천에서보다 균일사로 이루어진 하천에서 하상이 깊게 세굴되는 현상을 보여준다. 혼합사로 이루어진 하천에서의 공간적 입도 분포는 사행하천에서와 마찬가지로 하상이 퇴적되는 사행의 내측에서는 세립토로 구성되고 하상이 세굴되는 외측에서는 굵은 입자로 구성되는 것을 보여주었으며, 하천 폭이 좁은 부분에서는 하상의 세출 및 하상재료가 굵게 변화되는 것을 보여주었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제36권6호
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• pp.528-532
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• 2010

Introduction: There are few reports on tooth eruption through Bio-Oss grafts. To our knowledge, there are no reports on whether teeth can erupt normally through the grafts. The aim of this study was to examine the effect of Bio-Oss grafts on tooth eruption in a canine model. Materials and Methods: In five 10-week-old dogs, the deciduous third mandibular molars in one jaw quadrant of each animal were extracted and the fresh extraction sockets were then filled with Bio-Oss particles (experimental side). No such treatments were performed on the contralateral side (control side). A clinical and radiological evaluation was carried out every other week to evaluate the eruption level of the permanent third mandibular premolars and compare the eruption levels between the two sides. Results: At week 4 after the experiment, the permanent third premolars began to erupt on both sides. At week 12, the crown of the permanent third premolar emerged from the gingiva on both sides. At week 20, the permanent third premolars on both sides erupted enough to occlude the opposing teeth. No significant differences were found between the control and experimental sides in terms of the eruption speed of the permanent third molars. Conclusion: These findings demonstrate that the grafting of Bio-Oss particles into the alveolar bone defects does not affect tooth eruption.

• 대한치과마취과학회지
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• 제3권2호
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• pp.92-97
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• 2003

Background: Gabapentin is a novel anti-epileptic drug, which is used in clinical practice to treat epilepsy. This drug is also used as an analgesic in pain patients. The antinociceptive effect of this drug was assessed using the formalin test in the rat. Methods: In order to investigate the effects of gabapentin on the trigeminal nerve territory, we injected 0.5% formalin into the upper lip. Adult, male, Sprague-Dawley rats received a $50{\mu}l$ subcutaneous injection of 5% formalin into one vibrissal pad and the consequent, facial grooming behavior was monitored. Consistent with previous investigations using tile formalin model, animals exhibited biphasic nocifensive grooming (phase 1, 0-12 min; phase 2, 12-60 min). Results: The intraperitoneal administration gabapentin 5 minutes prior to the formalin injection led to a significant, dose-dependent reduction in grooming time during phase 2. In high doses, gabapentin also reduced the time of grooming during phase 1. Conclusions: The Intraperitoneal injection of gabapentin has an analgesic effect in the facial formalin rat model and this analgesic effect increases dose-dependently.

• Archives of Plastic Surgery
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• 제34권6호
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• pp.777-784
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• 2007

Purpose: For a minor degree of mandibular prognathism, mandibular anterior segmental osteotomy (ASO), usually extracting the bilateral premolars, has been performed frequently to correct malocclusion of the anterior teeth. Preoperative planning using cephalometry and a dental model is very important for such a orthognathic surgery. Depending on the specific preoperative mock surgery with the dental model, ASO, with ipsilateral unitooth extraction, is defined to be feasible and performed for ten patients. The comparisons of its preoperative and postoperative analysis of clinical photographs, dental casts, and lateral cephalograms, for soft tissue profiles, skeletal and dental relationships are described in the following, and its clinical applications are noted. Methods: From March 1, 2004, to March 31, 2006, We performed 10 mandibular ASO by extraction of ipsilateral unitooth to improve their lower facial profiles and the lip relationships. Patient age ranged from 19 to 33 years, with a mean age of 25.6 years. Two were males and eight were females. Results: All patients were satisfied with aesthetic and occlusal changes postoperatively. Significant and persistent decrease in the SNB and interincisal angle were observed in the postoperative cephalometries. The soft tissue profiles also were improved and near Ricketts's esthetic line. Other combined procedures include nine genioplasties, two rhinoplasties, and one blepharoplasty. One patient complained of transient unilateral inferior mental nerve paresthesia. There were no other significant complications or relapses throughout the follow-up period(6-20 months). Conclusion: Mandibular ASO, extracting the ipsilateral unitooth, was performed for ten patients to correct mild mandibular prognathism. The amount of setback of the mandibular anterior portion was 2 to 3 mm, and satisfactory results were obtained combined with genioplasties.

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9241-9247
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• 2014

Background: Rs31489 in the cleft lip and palate transmembrane1-like gene (CLPTM1L) has been identified to be associated with lung cancer through genome-wide association studies (GWAS). However, some recent replication studies yielded inconclusive results. Thus, we undertook this study to investigate the precise effect of rs31489 on lung cancer susceptibility. Materials and Methods: A hospital-based case-control study in 1,673 Chinese subjects (611 individuals with lung cancer and 1,062 controls) and a meta-analysis among 32,199 subjects (16,364 cases and 15,835 controls) were performed in this study. Results: In our case-control study, rs31489 was inversely associated with lung cancer (AC versus CC: OR=0.68, 95%CI=0.52-0.88; additive model: OR=0.68, 95%CI=0.54-0.85; dominant model: OR=0.65, 95%CI =0.51-0.84). Stratification analysis by smoking status showed a significant association and strong genetic effect in non-smokers but not in smokers. Our meta-analysis further confirmed the association, although with significant heterogeneity contributed by study design and source of controls, as shown by stratified analysis. Sensitive and cumulative analyses both indicated robust stability of our results. In addition, there was no observable publication bias in our meta-analysis. Conclusions: Overall, the findings from our replication study and meta-analysis demonstrated that CLPTM1L gene rs31489 is significantly associated with lung cancer.

• 한국컴퓨터정보학회논문지
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• 제11권2호
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• pp.159-167
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• 2006

립리딩은 잡음이 있는 환경에서 음성 인식 시스템의 성능 향상을 위한 한 방법으로 제안되었다. 기존의 논문들이 소프트웨어 립리딩 방법을 제안하는 것에 반하여, 본 논문에서는 실시간 립리딩을 위한 하드웨어 설계를 제안한다. 실시간 처리와 구현의 용이성을 위하여 본 논문에서는 립리딩 시스템을 이미지 획득 모듈, 특징 벡터 추출 모듈, 인식 모듈의 세 모듈로 분할하였다. 이미지 획득 모듈에서는 CMOS 이미지 센서를 사용하여 입력 영상을 획득하게 하였고, 특징 벡터 추출 모듈에서는 병렬 블록매칭 알고리즘을 이용하여 입력영상으로부터 특징벡터를 추출하도록 하였고, 이를 FPGA로 코딩하여 시뮬레이션 하였다. 인식 모듈에서는 추출된 특징 벡터에 대하여 HMM 기반 인식 알고리즘을 적용하여 발성한 단어를 인식하도록 하였고, 이를 DSP에 코딩하여 시뮬레이션 하였다. 시뮬레이션 결과 실시간 립리딩 시스템이 하드웨어로 구현 가능함을 알 수 있었다.

• 대한두개안면성형외과학회지
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• 제12권2호
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• pp.86-92
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• 2011

Purpose: The purpose of this study was to investigate the possibility that a dynamic facial composite flap with sensory and motor nerves could be made available from donor facial composite tissue. Methods: The faces of 3 human cadavers were dissected. The authors studied the donor faces to assess which facial composite model would be most practicable. A "panorama facial flap" was excised from each facial skeleton with circumferential incision of the oral mucosa, lower conjunctiva and endonasal mucosa. In addition, the authors measured the available length of the arterial and venous pedicles, and the sensory nerves. In the recipient, the authors evaluated the time required to anastomose the vessels and nerve coaptations, anchor stitches for donor flaps, and skin stitches for closure. Results: In the panorama facial flap, the available anastomosing vessels were the facial artery and vein. The sensory nerves that required anastomoses were the infraorbital nerve and inferior alveolar nerve. The motor nerve requiring anstomoses was the facial nerve. The vascular pedicle of the panorama facial flap is the facial artery and vein. The longest length was 78 mm and 48 mm respectively. Sensation of the donor facial composite is supplied by the infraorbital nerve and inferior alveolar nerve. Motion of the facial composite is supplied by the facial nerve. Some branches of the facial nerve can be anastomosed, if necessary. Conclusion: The most practical facial composite flap would be a mid and lower face flap, and we proposed a panorama facial flap that is designed to incorporate the mid and lower facial skin with and the unique tissue of the lip. The panorama facial composite flap could be considered as one of the practicable basic models for facial allotransplantation.

• The Korean Journal of Physiology and Pharmacology
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• 제25권4호
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• pp.365-374
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• 2021

The mammalian target of rapamycin (mTOR) plays a role in various cellular phenomena, including autophagy, cell proliferation, and differentiation. Although recent studies have reported its involvement in nociceptive responses in several pain models, whether mTOR is involved in orofacial pain processing is currently unexplored. This study determined whether rapamycin, an mTOR inhibitor, reduces nociceptive responses and the number of Fos-immunoreactive (Fos-ir) cells in the trigeminal nucleus caudalis (TNC) in a mouse orofacial formalin model. We also examined whether the glial cell expression and phosphorylated p38 (p-p38) mitogen-activated protein kinases (MAPKs) in the TNC are affected by rapamycin. Mice were intraperitoneally given rapamycin (0.1, 0.3, or 1.0 mg/kg); then, 30 min after, 5% formalin (10 μl) was subcutaneously injected into the right upper lip. The rubbing responses with the ipsilateral forepaw or hindpaw were counted for 45 min. High-dose rapamycin (1.0 mg/kg) produced significant antinociceptive effects in both the first and second phases of formalin test. The number of Fos-ir cells in the ipsilateral TNC was also reduced by high-dose rapamycin compared with vehicle-treated animals. Furthermore, the number of p-p38-ir cells the in ipsilateral TNC was significantly decreased in animals treated with high-dose rapamycin; p-p38 expression was co-localized in microglia, but not neurons and astrocytes. Therefore, the mTOR inhibitor, rapamycin, reduces orofacial nociception and Fos expression in the TNC, and its antinociceptive action on orofacial pain may be associated with the inhibition of p-p38 MAPK in the microglia.

• 정보처리학회 논문지
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• 제13권4호
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• pp.189-198
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• 2024

입 모양 인식은 음성 인식의 중요 부분 중 하나로 음성 인식을 위한 입 모양 인식 시스템에서 입 모양 인식 성능을 개선하기 위한 여러 연구가 진행됐다. 최근의 연구에서는 인식 성능을 개선하기 위해 입 모양 인식 시스템의 모델 구조를 수정하는 방법이 사용됐다. 본 연구에서는 모델 구조를 수정하는 것으로 인식 성능을 개선하는 기존의 연구와 달리 모델 구조의 변화 없이 인식 성능을 개선하는 것을 목표로 한다. 모델 구조의 수정 없이 인식 성능을 개선하기 위해, 사람이 하는 입 모양 인식에서 사용되는 단서를 참고해 입 모양 인식 시스템의 기존 관심 영역인 입술 영역과 함께 턱, 뺨과 같은 다른 영역을 관심 영역으로 설정하고 각 관심 영역의 인식률을 비교해 가장 높은 성능의 관심 영역을 제안한다. 또한, 관심 영역 크기를 정규화하는 과정에서 보간법의 차이로 인해 발생하는 정규화 결과의 차이가 인식 성능에 영향을 준다고 가정하고 최근접 이웃 보간법, 이중 선형 보간법, 이중 삼차 보간법을 사용해 동일한 관심 영역을 보간하고 각 보간법에 따른 입 모양 인식률을 비교해 가장 높은 성능의 보간법을 제안한다. 각 관심 영역은 객체 탐지 인공신경망을 학습시켜 검출하고, 각 관심 영역을 정규화하고 특징을 추출하고 결합한 뒤, 결합된 특징들을 차원 축소한 결과를 저차원 공간으로 매핑하는 것으로 동적 정합 템플릿을 생성했다. 생성된 동적 정합 템플릿들과 저차원 공간으로 매핑된 데이터의 거리를 비교하는 것으로 인식률을 평가했다. 실험 결과 관심 영역의 비교에서는 입술 영역만을 포함하는 관심 영역의 결과가 이전 연구의 93.92%의 평균 인식률보다 3.44% 높은 97.36%의 평균 인식률을 보였으며, 보간법의 비교에서는 이중 선형 보간법이 97.36%로 최근접 이웃 보간법에 비해 14.65%, 이중 삼차 보간법에 비해 5.55% 높은 성능을 나타내었다. 본 연구에 사용된 코드는 https://github.com/haraisi2/Lipreading-Systems에서 확인할 수 있다.