• Title/Summary/Keyword: lines detection

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Molecular Emission Spectrometric Detection of Low Level Sulfur Using Hollow Cathode Glow Discharge

  • Koo, Il-Gyo;Lee, Woong-Moo
    • Bulletin of the Korean Chemical Society
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    • v.25 no.1
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    • pp.73-78
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    • 2004
  • A highly sensitive detecting method has been developed for determining part per billion of sulfur in $H_2S$/Ar plasma. The method is based on the excitation of Ar/$H_2S\;or\;Ar/H_2S/O_2$ mixture in hollow cathode glow discharge sustained by radiofrequency (RF) or 60 Hz AC power and the spectroscopic measurement of the intensity of emission lines from electronically excited $S_2^*\;or\;SO_2^*$ species, respectively. The RF or AC power needed for the excitation did not exceed 30 W at a gas pressure maintained at several mbar. The emission intensity from the $SO_2^*$ species showed excellent linear response to the sulfur concentration ranging from 5 ppbv, which correspond to S/N = 5, to 500 ppbv. But the intensity from the $S_2^*$ species showed a linear response to the $H_2S$ only at low flow rate under 20 sccm (mL/min) of the sample gas. Separate experiments using $SO_2$ gas as the source of sulfur demonstrated that the presence of $O_2$ in the argon plasma is essential for obtaining prominent $SO_2^*$ emission lines.

Live Cell Detection of Monoclonal Antibody Light and Heavy Chain mRNAs using Molecular Beacons (분자 비컨을 이용한 살아 있는 세포에서 단일클론항체 경쇄와 중쇄 mRNA 검출에 의한 세포주 선별방법)

  • Jeong, Seunga;Rhee, Won Jong
    • KSBB Journal
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    • v.31 no.1
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    • pp.33-39
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    • 2016
  • Developing the method for the selection of animal cell line producing therapeutic monoclonal antibody (mAb) is invaluable as its market is rapidly growing. Although the quality of produced mAb is as important as quantity, however there is no method developed for the selective screening of cell lines on the basis of both quantity and quality. From recent reports, the ratio of light and heavy chain mRNAs of mAb in the cell is a key parameter for the indication of product quality. Therefore, it is obvious that developing the novel method that can detect both light and heavy chain mRNAs in single live cell will provide unprecedented opportunities in bio-industry. Here, we have constructed oligonucleotide probes, molecular beacons for the detection of light or heavy chain mRNAs, respectively, in the live cells producing mAbs. Both beacons showed increased fluorescent intensity after transient transfection of plasmid expressing mAbs analyzed by fluorometer. Flow cytometric analysis clearly demonstrated that both molecular beacons can simultaneously detect the expression of light and heavy chain mRNAs of mAb in the same cell. The technique described in the thesis provides the new direction and concept for developing the method for the smart selection of cell lines producing recombinant proteins including therapeutic mAbs.

Detection of QTLs Influencing Panicle Length, Panicle Grain Number and Panicle Grain Sterility in Rice(Oryza sativa L.)

  • Ahamadi, Jafar;Fotokian, M.H.;Fabriki-Orang, S.
    • Journal of Crop Science and Biotechnology
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    • v.11 no.3
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    • pp.163-170
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    • 2008
  • The detection, characterization and use of quantitative traits loci, QTL, have significant potential to improve the efficiency of selective breeding of species. Therefore, a population with 59 advanced backcross lines($BC_2F_5$), derived from a cross between IR64 and Tarome molaei, were studied in Tonekabon Rice Research Station of Iran in order to map QTLs for panicle length, number of grain per panicle, and panicle grain sterility in rice. The parental screening wtih 235 SSR markers in agarose and polyacrylamide gels revealed 114 markers with clear polymorphic bands. To search for QTLs associated with panicle length, number of grain per panicle, and panicle grain sterility, we constructed a genetic linkage map using 114 microsatellite markers. Positive and negative transgressive segregations were observed in $BC_2F_5$ lines for all traits. Using multiple interval mapping(MIM), a total of 20 putative QTLs were detected, of which eight were for panicle length, three for number of grains, and nine for panicle grain sterility. The maximum number of QTLs were mapped on chromosomes 1 and 2 with eight QTLs. These QTL markers could possible be utilized for marker-assisted selection.

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Electric Fire Prediction by Detection of Discharge Signal (방전신호 검출에 의한 전기화재 예측)

  • 길경석;송재용;권장우
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.2
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    • pp.413-419
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    • 2004
  • This paper describes a technique that can predict electric fires by detection of discharge voltage signals caused by the use of electric facilities. In the experiment, various discharge modes, a flashover or a surface discharge through insulation paper and a line to line short, were simulated to acquire electrical information for predicting electrical fire as discharge modes. From the experimental results, it is hewn that electorial discharges which are ranked as majority causes of electric fires generate characterized signals distinguished from power frequency. Finally. We designed a prototype discharge detector based on the experimental results, and the detector is applied to a power lines. This study showed that the prediction of electric fires is possible by monitoring discharge voltage signals in electric power lines.

"Dust, Ice, and Gas In Time" (DIGIT) Herschel Observations of GSS30-IRS1 in Ophiuchus

  • Je, Hyerin;Lee, Jeong-Eun;Green, Joel D.;Evans, Neal J. II
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.1
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    • pp.63.2-63.2
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    • 2014
  • As a part of the "Dust, Ice, and Gas In Time" (DIGIT) key program on Herschel, we observed GSS30-IRS1, a Class I protostar located in Ophiuchus (d =125 pc), with Herschel/Photodetector Array Camera and Spectrometer (PACS). More than 70 lines were detected within a wavelength range from 50 ${\mu}m$ to 200 ${\mu}m$: CO lines from J = 14-13 to 41-40, several $H_2O$ lines of Eup = 100 K to 1500 K, 16 transitions of OH rotational lines, and two atomic [O I] lines at 63 and 145 ${\mu}m$. The [C II] line, known as a tracer of externally heated gas by the interstellar radiation field, is also detected at 158 ${\mu}m$. All lines, except [O I] and [C II], are detected only at the central spaxel of $9^{\prime\prime}.4{\times}9^{\prime\prime}.4$. The [O I] emission is extended along a NE-SW orientation, which is consistent with the known outflow direction, while the [C II] line is detected over all spaxels. One possible explanation of the detection of the [C II] line and no correlation of its spatial distribution with any other molecular emission is the existence of the enhanced ISRF nearby GSS30-IRS1. One interesting feature of GSS30-IRS1 is that the continuum emission is extended beyond the point-spread function (PSF), unlike the molecular line emission, indicative of significant external heating. The best-fit continuum model of GSS30-IRS1 with the physical structure including flared disk, envelope, and outflow shows that the internal luminosity is 11 $L_{\odot}$, and the region is also externally heated by a radiation field enhanced by a factor of 25 compared to the local standard interstellar field.

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Fast Skew Detection of Document Images by Extraction of Center Points of Blank Lines (공백행의 중심점 추출에 의한 고속 문서 기울기 검출)

  • Jeong, Jae-Yeong;Kim, Mun-Hyeon
    • Journal of KIISE:Software and Applications
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    • v.26 no.11
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    • pp.1342-1349
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    • 1999
  • 본 논문에서는 문서 내의 인접한 두 행 사이에는 일정한 두께의 공백 행이 존재하며 그 공백 행의 기울기는 실제 문서의 기울어진 정도를 반영한다는 사실에 기반하여, 선형적으로 기울어진 문서 영상의 기울기 추정을 위한 고속의 알고리즘을 제안한다. 먼저, 간단한 모폴로지 연산(dilation)을 이용하여 문자행 영역과 공백행 영역을 분리한 후, 이를 일정 간격으로 수직 샘플링하여 수직선 상에 있는 모든 공백행의 중심점(행간점)을 찾는다. 동일한 공백 행 상에 있는 인접한 두 행간점 간에 기울기를 계산하고, 전체 영상으로부터 이들의 분포를 조사하여 최대 빈도를 가지는 기울기를 입력 문서의 기울기로 추정한다. 실험에서는 제안한 알고리즘을 필기체 및 인쇄체를 포함하는 다양한 형태의 가로쓰기 문서에 적용한 결과를 보인다.Abstract In this paper, we propose a fast algorithm to estimate the skew angle of linearly skewed document images. This paper is based on the fact that there is a blank line with uniform thickness between two adjacent text lines and the slope of the line is the same as that of the document. Firstly, we apply a dilation operation to the image to separate blank lines from text lines, and we detect center points of blank lines along the vertically sampled lines. Then we calculate the slope between neighboring center points in the same blank line. Calculated slopes for the entire image are accumulated on the histogram to display the distribution of them. Finally, the peak in the histogram is detected and estimated as the slope of the document image. In the experiments, we adopted a lot of images of various format with hand-printed or machine-printed document to verify our algorithm.

Metamaterial CRLH Structure-based Balun for Common-Mode Current Indicator

  • Kahng, Sungtek;Lee, Jinil;Kim, Koon-Tae;Kim, Hyeong-Seok
    • Journal of Electrical Engineering and Technology
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    • v.9 no.1
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    • pp.301-306
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    • 2014
  • We proposed a new PCB-type 'common-mode current($I_c$) and differential-mode current($I_d$) detector' working for fast detection of $I_c$ and $I_d$ from the differential-mode signaling, with miniaturization effect and possibility of cheaper fabrication. In order to realize this device, we suggest a branch-line-coupler balun having a composite right- and left-handed(CRLH) one-layer microstrip phase-shifting line as compact as roughly ${\lambda}_g/14$. The presented balun obviously is different from the conventional bent-&-folded delay lines or slits on the ground for coupling the lines on the top and bottom dielectrics. As we connect the suggested balun output ports of the differential-mode signal lines via the through-port named U and coupled-port named L, $I_c$ and $I_d$ will appear at port ${\Delta}$ and port ${\Sigma}$ of the present device, in order. The validity of the design scheme is verified by the circuit-and numerical electromagnetic analyses, and the dispersion curve proving the metamaterial characteristics of the geometry. Besides, the examples of the $I_c$ and $I_d$ indicator are observed as the even and odd modes in differential-mode signal feeding. Also, the proposed device is shown to be very compact, compared with the conventional structure.

Trend Properties and a Ranking Method for Automatic Trend Analysis (자동 트렌드 탐지를 위한 속성의 정의 및 트렌드 순위 결정 방법)

  • Oh, Heung-Seon;Choi, Yoon-Jung;Shin, Wook-Hyun;Jeong, Yoon-Jae;Myaeng, Sung-Hyon
    • Journal of KIISE:Software and Applications
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    • v.36 no.3
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    • pp.236-243
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    • 2009
  • With advances in topic detection and tracking(TDT), automatic trend analysis from a collection of time-stamped documents, like patents, news papers, and blog pages, is a challenging research problem. Past research in this area has mainly focused on showing a trend line over time of a given concept by measuring the strength of trend-associated term frequency information. for detection of emerging trends, either a simple criterion such as frequency change was used, or an overall comparison was made against a training data. We note that in order to show most salient trends detected among many possibilities, it is critical to devise a ranking function. To this end, we define four properties(change, persistency, stability and volume) of trend lines drawn from frequency information, to quantify various aspects of trends, and propose a method by which trend lines can be ranked. The properties are examined individually and in combination in a series of experiments for their validity using the ranking algorithm. The results show that a judicious combination of the four properties is a better indicator for salient trends than any single criterion used in the past for ranking or detecting emerging trends.

Fast Detection of Power Lines Using LIDAR for Flight Obstacle Avoidance and Its Applicability Analysis (비행장애물 회피를 위한 라이다 기반 송전선 고속탐지 및 적용가능성 분석)

  • Lee, Mijin;Lee, Impyeong
    • Spatial Information Research
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    • v.22 no.1
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    • pp.75-84
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    • 2014
  • Power lines are one of the main obstacles causing an aircraft crash and thus their realtime detection is significantly important during flight. To avoid such flight obstacles, the use of LIDAR has been recently increasing thanks to its advantages that it is less sensitive to weather conditions and can operate in day and night. In this study, we suggest a fast method to detect power lines from LIDAR data for flight obstacle avoidance. The proposed method first extracts non-ground points by eliminating the points reflected from ground surfaces using a filtering process. Second, we calculate the eigenvalues for the covariance matrix from the coordinates of the generated non-ground points and obtain the ratio of eigenvalues. Based on the ratio of eigenvalues, we can classify the points on a linear structure. Finally, among them, we select the points forming horizontally long straight as power-line points. To verify the algorithm, we used both real and simulated data as the input data. From the experimental results, it is shown that the average detection rate and time are 80% and 0.2 second, respectively. If we would improve the method based on the experiment results from the various flight scenario, it will be effectively utilized for a flight obstacle avoidance system.

A Study on Detection of Lane and Situation of Obstacle for AGV using Vision System (비전 시스템을 이용한 AGV의 차선인식 및 장애물 위치 검출에 관한 연구)

  • 이진우;이영진;이권순
    • Journal of Korean Port Research
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    • v.14 no.3
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    • pp.303-312
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    • 2000
  • In this paper, we describe an image processing algorithm which is able to recognize the road lane. This algorithm performs to recognize the interrelation between AGV and the other vehicle. We experimented on AGV driving test with color CCD camera which is setup on the top of vehicle and acquires the digital signal. This paper is composed of two parts. One is image preprocessing part to measure the condition of the condition of the lane and vehicle. This finds the information of lines using RGB ratio cutting algorithm, the edge detection and Hough transform. The other obtains the situation of other vehicles using the image processing and viewport. At first, 2 dimension image information derived from vision sensor is interpreted to the 3 dimension information by the angle and position of the CCD camera. Through these processes, if vehicle knows the driving conditions which are lane angle, distance error and real position of other vehicles, we should calculate the reference steering angle.

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