• 한국동물위생학회지
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• 제32권3호
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• pp.281-286
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• 2009

Fluoroquinolones in muscle and egg were separated by liquid extraction and determined. The analysis was carried out using following conditions; C18 column ($150{\times}4.6mm$, $5{\mu}m$), mobile phase composed of D.W. (containing 0.4% triethylamine and phosphoric acid) : methanol : acetonitrile (780:100:120, v/v/v), quarternary pump at a flow rate of 0.9ml/min and $20{\mu}l$ of injection volume, fluorescence detector with EX 278nm/Em 456nm. The calibration range of seven fluoroquinolones showed linearity ($r^2{\geq}0.999$) at concentration range of $0.025{\sim}0.8{\mu}g/ml$. The recoveries in fortified muscle and egg represented more than 81.3%. The detection limits for ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, saraloxacin and orbifloxacin were 3.1, 2.5, 3.6, 1.7, 0.9, 2.5 and $2.1{\mu}g/kg$, respectively. We also monitored fluoroquinolones residue in the sample (chicken muscle 182, cattle muscle 140, pig muscle 139, egg 212) using EEC-plate (E. coli ATCC 11303) screening and HPLC confirmation methods. The screening test results, fluoroquinolones, antibacterial substances were all negative.

• 한국동물위생학회지
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• 제40권4호
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• pp.253-258
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• 2017

The aim of this study is to develop an optimal analytical method for gonadorelin, progesterone, oxytocin and estradiol, the major components of hormones. A relatively simple and reproducible method using high performance liquid chromatography was developed and as a result of the measurement of specificity, linearity, repeatability, accuracy and intermediated precision, the validity of the developed method was verified with the result of meeting the verification criteria of analytical method validation. Using this newly developed method, 12 post-market veterinary products were tested and the ingredient content were 91.9~116.4%, which satisfied the 90~120% condition of the administrative measure standard. Therefore, if the newly developed method is used for the collection examination of hormone in veterinary medicine, it can be useful as an approved test method.

• 천문학회보
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• 제39권2호
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• pp.91.1-91.1
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• 2014

IGRINS (the Immersion GRating INfrared Spectrometer) is a high resolution wide-band infrared spectrograph developed by the Korea Astronomy and Space Science Institute (KASI) and the University of Texas at Austin (UT). This spectrograph has H-band and K-band science cameras, both of which use Teledyne's $2.5{\mu}m$ cutoff $2k{\times}2k$ HgCdTe HAWAII-2RG CMOS science grade detectors. Teledyne's cryogenic SIDECAR ASIC boards and JADE2 USB interface cards were installed to control these detectors. We performed lab experiments and test observations to optimize and evaluate the detector systems of science cameras. In this presentation, we describe a process to optimize bias voltages and way to reduce pattern noise with reference pixel subtraction schemes. We also present measurements of the following properties under optimized settings of bias voltages at cryogenic temperature (70K): read noise, Fowler noise, dark current, and reference-level stability, full well depth, linearity and conversion gain.

• 방송공학회논문지
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• 제14권1호
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• pp.3-14
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• 2009

최근에 H.264/AVC 베이스라인 프로파일은 많은 멀티미디어 응용기기에 사용되고 있다. 또한 양방향 예측을 위한 B 픽쳐를 지원하는 MPEG-2 메인 프로파일은 HDTV, DVD와 같은 많은 멀티미디어 응용기기에 적용되어 왔다. 따라서 MPEG-2 메인 프로파일에서 H.264 베이스라인 프로파일로의 트랜스코딩은 콘텐츠의 범용성을 높이기 위해 필요한 작업이다. 양방향 움직임 추정을 지원하지 않는 H.264/AVC 베이스라인 프로파일로의 전환을 위해 Group of Pictures 구조 변환은 필수적이다. 이러한 Group of Pictures 구조 변환을 포함하는 트랜스코딩 구조에 있어서 제안한 알고리듬은 예측된 움직임 벡터의 선형성 검사를 통한 적응적인 탐색 범위선택과 참조 영역 복잡성 정보를 이용한 적응적 모드 선택 방법을 통해 화질 열화를 최소화하며 계산상의 복잡성을 획기적으로 줄였다.

• Natural Product Sciences
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• 제18권1호
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• pp.39-46
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• 2012

In Korea, the leaves of Sonchus brachyotus (Compositae), an edible mountainous vegetable, are traditionally used to treat hepatitis and hemorrhage and are known to have diuretic action. The aqueous ethanolic extract of this plant was selected in our screening experiment using the peroxynitrite ($ONO_2^-$)-scavenging assay, and the present study was performed to qualitatively and quantitatively identify the active compounds from S. brachyotus and validate the present high-permormance liquid chromatography (HPLC) coupled with ultraviolet absorption detection method based on accuracy, precision and repeatability. Five phenolic substances including the main compound, luteolin $7-O-{\beta}-D$-glucuronopyranoside, as well as chlorogenic acid, luteolin 7-O-rutinoside, luteolin $7-O-{\beta}-D$-glucopyranoside, and luteolin, were found in the aqueous ethanolic extract of S. brachyotus. In the HPLC validation experiment, the linearity of the four compounds was established by $R^2$ values of more than 0.999 within the test ranges, and the recovery rate ranged from 98.2 - 105.3%. Luteolin 7-O-glucuronide was a predominant compound (143 mg/g of extract and 18.3 mg/g of the dry weight of plant material) with a potent peroxynitrite-scavenging effect ($IC_{50}$, $1.02{\pm}0.08{\mu}M$). Luteolin and its three glycosides together with chlorogenic acid were qualitatively and quantitatively determined using an HPLC method validated in the present study.

• Bulletin of the Korean Chemical Society
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• 제32권7호
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• pp.2418-2422
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• 2011

The enantiomeric separation of lactic acid for its absolute configuration has become important task for understanding its biological origin and metabolic process involved in the formation of lactic acid. It involves the conversion of enantiomers as diastereomeric O-pentafluoropropionylated (S)-(+)-3-methyl-2-butyl ester and the direct separation by gas chromatography-mass spectrometry on a achiral capillary column. The (R)- and (S)-lactic acids were completely separated with a high resolution of 1.9. The newly developed method showed good linearity (r ${\geq}$ 0.999), precision (% relative standard deviation = 3.4-6.2), and accuracy (% relative error = -7.7-1.4) with the detection limit of 0.011 ${\mu}g/mL$. When the method was applied to determine the absolute configuration of lactic acid in Lactobacillus delbrueckii subsp. lactis 304 (LAB 304), the composition (%) of (R)-lactic acid in the cell pellet and in the culture medium were $89.0{\pm}0.1$ and $78.2{\pm}0.4$, respectively. Thus, it was verified that the present method is useful for the identification and composition test of lactic enantiomers in microorganisms.

• 한국산림과학회지
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• 제94권4호통권161호
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• pp.281-286
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• 2005

수목은 뿌리에 의한 토양수분의 제거와 기계적인 보강효과를 통해 토괴가 사면 하부로 이동하는 것을 막아 안정하게 하는 역할을 한다. 본 연구는 자연사면상에서 이런 보강효과를 정량화하기 위해, 표준사에 다른 형태로 수목의 뿌리를 내재시켜 직접전단시험을 실시하였으며, 공시뿌리로서는 잣나무가 이용되었다. 실험은 뿌리가 내재된 것과 그렇지 않은 토양에서 실시하였으며, 여러 수준의 수직구속 압력에서 최대 전단저항력을 측정하였다. 뿌리에 의해 증가된 토양전단저항력은 측정된 토양의 내부마찰각과 점착력 등의 토양변수를 이용하여 계산하였으며, 전단상자내의 뿌리배열에 의한 효과도 함께 분석하였다. 실험결과 전단저항력은 뿌리의 개수와 직경이 증가할수록 증가하였으며, 뿌리의 배열 형태가 십자 배열일 때 가장 큰 효과를 나타내었다. 또한 실험을 통해 확인한 전통적인 토양보강 모델과의 비교에서는 기존 모델의 선형성으로 실제 실험 결과와 일치하지 않는 경향을 나타내었다.

• 대한본초학회지
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• 제30권3호
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• pp.19-24
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• 2015

Objectives : HPL-1, a novel herbal medicine which is composed of five herbs such as Kalopanacis Cortex, Chaenomelis Fructus, Raphani Semen, Atractylodis Rhizoma and Pulvis Aconiti Tuberis Purificatum, was developed for treatment of osteoarthritis. This study is aimed to develop analytical method for consistent quality control of HPL-1 and validate chromatographic method. Methods : Chromatographic analysis was performed using ultra-high performance liquid chromatography - diode array detector (UHPLC-DAD) equipped with RP-amide column, column oven, and auto sampler. Marker compounds [protocatechuic acid, chlorogenic acid, liriodendrin, 3,5-dicaffeoylquinic acid, ${\beta}$-D-(3-O-sinapoyl)-fructofuranosyl-$\alpha$-D-(6-O-sinapoyl)glucopyranoside and benzoylmesaconine] were separated by step gradient elution of acetonitrile and 0.1% phosphoric acid/water. The method validation was evaluated by quantitative validation parameters of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) according to KFDA guideline.Results : An optimized method for six marker compounds in HPL-1 was established by UHPLC-DAD. The correlation coefficient (R²) with each calibration curve was greater than 0.99. The LOD and LOQ were within the range of 0.008-0.090 and $0.023-0.274{\mu}g/mL$, respectively. The relative standard deviation (RSD) of intra- and inter-day variability were less than 4.0%. The result of recovery test was range from 93.3-106.3% with RSD < 4.0%.Conclusions : These results suggest that the quantitative UHPLC method is precise, accurate, effective for quality evaluation of HPL-1. The method may also contribute to improve quality of crude drug preparations used for treatment of various diseases.

• 한국수자원학회논문집
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• 제31권2호
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• pp.123-132
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• 1998

비포화대에서의 지하수 오염원의 이송을 관측하는 것은 매우 어려운 거승로 알려져 있다. 본 연구에서는 함수량과 농도가 다른 시료를 이용하여 비포화 용존 오염원의 농도를 측정하기 위한 TDR의 적용가능성에 대한 검정실험을 수행하였다. 초기전자기파에 대한 TDR반향파의 감쇄정도를 이용하여 토양의 총전기전도도를 측정하게 되는데 이때 함수량이 일정할 경우 총전기전도도와 토양수의 농도관계는 선형관계를 유지한다는 가정을 기본으로한다. 본 연구에서는 세가지의 농도와 체적함수량을 갖는 시료를 성형하고 이때의 TDR 반향곡선을 측정하여 Dalton 등(1984), Topp 등(1988), Yanuka 등(1988), Zegelin 등 (1989)이 제안한 추정식으로 통초양전기전도도를 구하였다. 실험결과 Zegelin등이 제안한 식을 제외한 세 가지 식들은 매우 좋은 토양수의 농도와 총전기전도도의 선형관계를 나타내었다. 따라서 이 세가지 추정식들은 용존 오염원의 농도를 추정함에 있어 매우 유용한 식으로 판단되며 이 추정식들을 이용하는 TDR 비포화 용존 오염원 측정법은 실내실험과 현장실험에 있어 매우 유용하리라 판단된다.

• Natural Product Sciences
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• 제19권1호
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• pp.20-27
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• 2013

A simple high performance liquid chromatography - diode array detector (HPLC-DAD) method has been developed and validated for simultaneous determination of the six components (cinnamic acid, cinnamaldehyde, eugenol, atractylenolide I, atractylenolide III, and ergosterol) in Oryeongsan. In addition, identification of six marker compounds was conducted by a LC-MS/MS analysis. The six compounds in Oryeongsan were separated on Shishedo $C_{18}$ column (5 ${\mu}m$, $4.6{\times}250$ mm) at a column temperature of $30^{\circ}C$. The mobile phase was a mixture of 0.1% trifluoroacetic acid (TFA) water and acetonitrile employing gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was set at 205, 250, 280, and 330 nm. The developed method had good linearity ($R^2$ > 0.9997) and the limit of detection (LOD) and limit of quantification (LOQ) were observed within the ranges 0.01~0.15 and 0.05~0.45 ${\mu}g/mL$, respectively. The relative standard deviation (RSD) values of intra- and inter-day testing were indicated that less than 3% and 90.31~103.31% of accuracy. The results of recovery test were 90.56~106.72% and RSD range was measured from 0.84 to 2.95%. In conclusion, this HPLC-DAD method has been successfully applied to the simultaneous determination of six compounds in Oryeongsan samples.