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http://dx.doi.org/10.6116/kjh.2015.30.3.19.

Development of Quality Control Method for a Novel Herbal Medicine, HPL-1 using UHPLC  

Kim, Se-Gun (Department of Oriental Pharmacy, Wonkwang University)
Lamichhane, Ramakanta (Department of Oriental Pharmacy, Wonkwang University)
Lee, Kyung-Hee (Department of Oriental Pharmacy, Wonkwang University)
Jung, Hyun-Ju (Department of Oriental Pharmacy, Wonkwang University)
Publication Information
The Korea Journal of Herbology / v.30, no.3, 2015 , pp. 19-24 More about this Journal
Abstract
Objectives : HPL-1, a novel herbal medicine which is composed of five herbs such as Kalopanacis Cortex, Chaenomelis Fructus, Raphani Semen, Atractylodis Rhizoma and Pulvis Aconiti Tuberis Purificatum, was developed for treatment of osteoarthritis. This study is aimed to develop analytical method for consistent quality control of HPL-1 and validate chromatographic method. Methods : Chromatographic analysis was performed using ultra-high performance liquid chromatography - diode array detector (UHPLC-DAD) equipped with RP-amide column, column oven, and auto sampler. Marker compounds [protocatechuic acid, chlorogenic acid, liriodendrin, 3,5-dicaffeoylquinic acid, ${\beta}$-D-(3-O-sinapoyl)-fructofuranosyl-$\alpha$-D-(6-O-sinapoyl)glucopyranoside and benzoylmesaconine] were separated by step gradient elution of acetonitrile and 0.1% phosphoric acid/water. The method validation was evaluated by quantitative validation parameters of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) according to KFDA guideline.Results : An optimized method for six marker compounds in HPL-1 was established by UHPLC-DAD. The correlation coefficient (R2) with each calibration curve was greater than 0.99. The LOD and LOQ were within the range of 0.008-0.090 and $0.023-0.274{\mu}g/mL$, respectively. The relative standard deviation (RSD) of intra- and inter-day variability were less than 4.0%. The result of recovery test was range from 93.3-106.3% with RSD < 4.0%.Conclusions : These results suggest that the quantitative UHPLC method is precise, accurate, effective for quality evaluation of HPL-1. The method may also contribute to improve quality of crude drug preparations used for treatment of various diseases.
Keywords
Herbal medicine; quality control; UHPLC-DAD;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Jhun HJ, Ahn K, Lee SC. Estimation of the prevalence of osteoarthritis in Korean adults based on the data from the fourth Korea national health and nutrition examination survey. Anesth Pain Med. 2010 ; 5(3) : 201-6.
2 Peat G, McCarney R, Croft P. Knee pain and osteoarthritis in older adults: a review of community burden and current use of primary health care. Ann Rheum Dis. 2001 ; 60(2) : 91-7.   DOI
3 Hochberg MC, Altman RD, Brandt KD, Clark BM, Dieppe PA, Griffin MR, Moskowitz RW, Schnitzer TJ. Guidelines for the medical management of osteoarthritis. Part Ⅱ. Osteoarthritis of the knee. American College of Rheumatology. Arthritis Rheum. 1995 ; 38(11) : 1541-6.   DOI   ScienceOn
4 Ko BS, Kang EJ, Kim HK. Isolation of sinapic acid esters from Raphani semen. Kor J Pharmacogn. 2000 ; 31(4) : 434-7.
5 Behrens F, Shepard N, Mitchell N. Alterations of rabbit articular cartilage by intra-articular injections of glucocorticoids. J Bone Joint Surg Am. 1975 ; 57(1) : 70-6.   DOI
6 Hanpoong pharmaceutical company. Herbal composition for treatment of rheumatoid arthritis and osteoarthritis. 10-1321754. 2013 : 1-21.
7 Herbal medical professor coeditorship. Herbal medicine. Seoul : Younglimsa. 1999 : 1-775.
8 Wang Y, Zhou J, Fu S, Wang C, Zhou B. Preventive Effects of Protocatechuic Acid on LPS-Induced Inflammatory Response in Human Gingival Fibroblasts via Activating PPAR-γ. Inflammation. 2015 ; 38(3) : 1080-4.   DOI
9 Chen WP, Wu LD. Chlorogenic acid suppresses interleukin-1β-induced inflammatory mediators in human chondrocytes. Int J Clin Exp Pathol. 2014 ; 7(12) : 8797-801.
10 Jung HJ, Park HJ, Kim RG, Shin KM, Ha J, Choi JW, Kim HJ, Lee YS, Lee KT. In vivo anti-inflammatory and antinociceptive effects of liriodendrin isolated from the stem bark of Acanthopanax senticosus. Planta Med. 2003 ; 69(7) : 610-6.   DOI   ScienceOn
11 Park KH, Park M, Choi SE, Jeong MS, Kwon JH, Oh MH, Choi HK, Seo SJ, Lee MW. The anti-oxidative and anti-inflammatory effects of caffeoyl derivatives from the roots of Aconitum koreanum R. RAYMOND. Biol Pharm Bull. 2009 ; 32(12) : 2029-33.   DOI   ScienceOn
12 Cho N, Huh J, Yang H, Jeong EJ, Kim YC, Kim J, Sung SH. Chemical constituents of Polygala tenuifolia roots and their inhibitory activity on lipopolysaccharide-induced nitric oxide production in BV2 microglia. J Enzyme Inhib Med Chem. 2012 ; 27(1) : 1-4.   DOI   ScienceOn
13 Kim OS, Seo CS, Shin HK. Simultaneous Determination of Gumiganghwal-tang and Its Anti-atherosclerotic Effect. Kor J Orient Pre Med Soc. 2013 ； 17(3) ： 165-76.
14 Suzuki Y, Oyama T, Ishige A, Isono T, Asami A, Ikeda Y, Noguchi M, Omiya Y. Antinociceptive mechanism of the aconitine alkaloids mesaconitine and benzoylmesaconine. Planta Med. 1994 ; 60(5) : 391-4.   DOI   ScienceOn
15 Jo JE, Lee AY, Kim HS, Moon BC, Choi G, Ji Y, Kim HK. Content Comparative Analysis and Classification for Piniellia ternate, P. pedatisecta and Typhonium flagelliforme by HPLC-PDA analysis. Kor J Herbol. 2013 ； 28(5) ： 95-101.   DOI   ScienceOn
16 Park JH, Jung JH, Park SI, Park SS. Studies on physical and chemical quality evaluation of crude drugs preparations analysis of So Cheong Ryong Tang. Kor J Pharmacogn. 1998 ; 29(1) : 35-9.
17 Seo CS, Kim OS, Shin HK. Quantitative Determination and Antioxidant Effects of Cheonwangbosimdan. Kor J Pharmacogn. 2014 ; 45(4) : 300-14.
18 Kwon YJ, Jang GC, Rha HH, Kim YH, Rhee MS. The study of sugar analysis in licorice extract by HPLC. J Kor Soc Tobacco Sci. 2005 ; 27(1) : 114-9.
19 Ko J, Lee MS, Kwak BM, Ahn JH, Park JS, Kwon JH. Determination of Isomaltooligosaccharides in Yoghurts by Using HPLC-ELSD. Korean J Food Sci An. 2013 ; 33(3) : 417-24.   DOI   ScienceOn
20 Kim SG, Poudel A, Kim YK, Jo HK, Jung HJ. Development of simultaneous analysis for marker constituents in Hwangryunhaedok-tang and its application in commercial herbal formulas. J Nat Med. 2013 ; 67(2) : 390-8.   DOI   ScienceOn
21 Kim OS, Seo CS, Shin HK. Simultaneous Determination of Gumiganghwal-tang and Its Anti-atherosclerotic Effect. Kor J Orient Pre Med Soc. 2013 ; 17(3) ： 165-76.
22 Miranda TA, Silva PH, Pianetti GA, César IC. Simultaneous quantitation of chloroquine and primaquine by UPLC-DAD and comparison with a HPLC-DAD method. Malar J. 2015 ; 14(1) : 29.   DOI   ScienceOn