Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Determination and survey of fluoroquinolones in meats and eggs (II)

식육 및 계란에서 플루오로퀴놀론계 항균물질 정량분석 및 잔류조사 (II)

  • Choi, Yoon-Hwa (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Yeon-Ju (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Kyung-Hye (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kang, Young-Il (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Jung-Hark (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 최윤화 (서울특별시 보건환경연구원) ;
  • 김연주 (서울특별시 보건환경연구원) ;
  • 이경혜 (서울특별시 보건환경연구원) ;
  • 강영일 (서울특별시 보건환경연구원) ;
  • 이정학 (서울특별시 보건환경연구원)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Fluoroquinolones in muscle and egg were separated by liquid extraction and determined. The analysis was carried out using following conditions; C18 column ($150{\times}4.6mm$, $5{\mu}m$), mobile phase composed of D.W. (containing 0.4% triethylamine and phosphoric acid) : methanol : acetonitrile (780:100:120, v/v/v), quarternary pump at a flow rate of 0.9ml/min and $20{\mu}l$ of injection volume, fluorescence detector with EX 278nm/Em 456nm. The calibration range of seven fluoroquinolones showed linearity ($r^2{\geq}0.999$) at concentration range of $0.025{\sim}0.8{\mu}g/ml$. The recoveries in fortified muscle and egg represented more than 81.3%. The detection limits for ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, saraloxacin and orbifloxacin were 3.1, 2.5, 3.6, 1.7, 0.9, 2.5 and $2.1{\mu}g/kg$, respectively. We also monitored fluoroquinolones residue in the sample (chicken muscle 182, cattle muscle 140, pig muscle 139, egg 212) using EEC-plate (E. coli ATCC 11303) screening and HPLC confirmation methods. The screening test results, fluoroquinolones, antibacterial substances were all negative.

Keywords

References

  1. 농림부 농업연수원.2007. 축산물잔류물질검사과정: 236-239
  2. 식품의약품안전청. 2008. 식품 중 동물용의약품 잔류허용기준 및 시험법 제개정
  3. 연합뉴스. 2007. 서울시, 여름철 먹을거리 안전성 검사 강화
  4. 조희정. 2007. 국내 축산식품 중 퀴놀론계 항생제의 찬류에 관한 연구. 건국대학교 대학원 석사학위논문.1-48
  5. 최윤화. 2009. 액체크로마토그래피-형광검출기를 이용한 식육 및 계란에서 플루오로퀴놀론제 항균물질 동시분석 및 잔류조사. 경북대학교 대학원 석사학위논문. 1-25
  6. Barry C. 1993. The analytical testing followed by laboratory services devision of agriculture Canada for veterinary drug residues in eggs. In proceedings of the euroresidues II conference on residues of veterinary drugs in food, Veldhoven, The Netherlands 3-5 May: 170-175
  7. Eliopoulos GM, Eliopoulos CT. 1993. Activity in vitro of the quinolones. In Hooper DC (ed)., Quinolone antimicrobial agents. 2nd ed. American Society for Microbiology, Washington: 161-193
  8. EMEA/MRL/820/02-Final. 2002. The European agency for the evaluation of medical products. Veterinary medicines and inspections, Comrnittee for vεterinary medical products, Enrofloxacin, Summary report (5)
  9. FDA. 2003. Approved animal drug products. NADA. 140-828.21 CFR 520.8 13.
  10. Gaugain JM, Abjean JP. 1998. Screening of quinolone residues in pig muscIe by planar chromatography. Chromatographia 47(1/2): 101-104 https://doi.org/10.1007/BF02466795
  11. Gellert M. 1981. DNA topoisomerase. Annu Rev Biochem 50: 879-910 https://doi.org/10.1146/annurev.bi.50.070181.004311
  12. Herikstad H, Hayes P, Mokhtar M, Fracaro ML, Threlfall EJ, Angulo FJ. 1997. Emerging quinolone-resistant Salmonella in the United States. Emerg Infect Dis 3(3): 371-372 https://doi.org/10.3201/eid0303.970316
  13. Horie M, Saito K, Nose N, Nakazawa H. 1994. Simultaneous determination of benofloxacin, danofloxacin, enrofloxacin and ofloxacin in chicken tissue by high-performance liquid chromatography. J Chromatogr B Biomed AppI 653(1): 69-76 https://doi.org/10.1016/0378-4347(93)E0428-S
  14. ICH. 1996. Topic Q2B. Validation of analytical procedure methodology in International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) Q2B Methodology guideline
  15. Kang HG, Son SW, Lee HS, Kim JH, Cho MH. 1997. Matrix Solid phase Dispersion (MSPD) extraction and HPLC determination of enrofloxacin and ciprofloxacin in pork muscIe tissue. Korean J Vet Res 37: 195-202
  16. Mitsuyama J. 1999. Structures of existing and new quinolones and relationship to bactericidal activity against Streptococcus pneumoniae. J Antimicrob Chemother 44(2): 201-207 https://doi.org/10.1093/jac/44.2.201
  17. Sara B, Giuseppe D, Antonio DC, Aldo L, Simone N. 2008. A simple and rapid assay based on hot water extraction and liquid chromatography-tandem mass spectrometry for monitoring quinolone residues in bovine milk. Food Chemistry 108(1): 354-360 https://doi.org/10.1016/j.foodchem.2007.10.044
  18. Schoevers EJ, van Leengoed LA, Verheijden JH, Niewold TA. 1999. Effects of enrofloxacin on porcine phagocytic function. Antimicrob Agents Chemother 43(9): 2138-2143
  19. Seo KW, Lee JI, Lee CY, Kim ES, Lee JC. 2002. Matrix Solid-Phase Dispersion (MSPD) for the isolation and LC determination of fluoroquinolones in eggs. Kor J Vet Publ Hlth 26(4): 269-281
  20. Smith KE, Besser JM, Hedberg CW, Leano FT, Bender JB, Wicklund JH, Johnson BP, Moore KA, Osterholm MT. 1999. Quinolone-resistant Campylobacter jejuni infections in Minnesota, 1992-1998. Investigation Team. N Engl J Med 340(20): 1525-1532 https://doi.org/10.1056/NEJM199905203402001