• Journal of Microbiology and Biotechnology
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• v.22 no.7
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• pp.1029-1033
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• 2012

A number of evidence have been accumulated that the regulation of reversible tyrosine phosphorylation, which can be regulated by the combinatorial activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), plays crucial roles in various biological processes including differentiation. There are a total of 107 PTP genes in the human genome, collectively referred to as the "PTPome." In this study, we performed PTP profiling analysis of the HIB-1B cell line, a brown preadipocyte cell line, during brown adipogenesis. Through RT-PCR and real-time PCR, several PTPs showing differential expression pattern during brown adipogenesis were identified. In the case of PTP-RE, it was shown to decrease significantly until 4 days after brown adipogenic differentiation, followed by a dramatic increase at 6 days. The overexpression of PTP-RE led to decreased brown adipogenic differentiation via reducing the tyrosine phosphorylation of the insulin receptor, indicating that PTP-RE functions as a negative regulator at the early stage of brown adipogenesis.

• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.330-336
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• 2020

Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.

• Annals of Surgical Treatment and Research
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• v.95 no.5
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• pp.240-248
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• 2018

Purpose: This study aimed to validate the synergistic effect of ABT-737 on docetaxel using MDA-MB-231, a triple negative breast cancer (TNBC) cell line overexpressing B-cell lymphoma-2 (Bcl-2). Methods: Western blot analysis was performed to assess expression levels of Bcl-2 family proteins and caspase-related molecules. Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle distribution was determined by flow cytometry analysis. Benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (z-VAD-fmk) was used for pretreatment to assess the role of caspases. Results: Cell viability of MDA-MB-231 after combination treatment with ABT-737 and docetaxel was significantly lower than that after docetaxel or ABT-737 monotherapy based on MTT assay (both P < 0.001), with a combination index of 0.41. The proportion of sub-G1 population after combination treatment was significantly higher than that after docetaxel or ABT-737 monotherapy (P = 0.001, P = 0.003, respectively). Pretreatment with z-VAD-fmk completely restored cell viability of MDA-MB-231 from apoptotic cell death induced by combination therapy (P = 0.001). Although pro-caspase-8 or Bid did not show significant change in expression level, pro-casepase-9 showed significantly decreased expression after combination treatment. Cleaved caspase-3 showed increased expression while poly (ADP-ribose) polymerase cleavage was induced after combination treatment. However, hypoxia-inducible factor 1-alpha and aldehyde dehydrogenase 1 totally lost their expression after combination treatment. Conclusion: Combination of ABT-737 with docetaxel elicits synergistic therapeutic effect on MDA-MB-231, a TNBC cell line overexpressing Bcl-2, mainly by activating the intrinsic pathway of apoptosis. Therefore, adjunct of ABT-737 to docetaxel might be a new therapeutic option to overcome docetaxel resistance of TNBCs overexpressing Bcl-2.

• Korean Journal of Psychosomatic Medicine
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• v.30 no.2
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• pp.80-98
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• 2022

Posttraumatic stress disorder (PTSD) is well known to have a limited response to drug treatment. Many recently published clinical care guidelines recommend trauma-focused psychotherapies such as cognitive processing therapy (CPT) and prolonged exposure therapy (PE) as first-line treatment and medication such as serotonin reuptake inhibitors and venlafaxine as second-line treatment. Current review introduces the session composition and contents of CPT and presents various CPT studies that show therapeutic effect for civilian and veterans/military with PTSD. In order for clinicians to help effectively patients with PTSD, it is necessary to learn and actively use evidence-based trauma-focused psychotherapies including CPT and PE.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.33 no.4
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• pp.346-351
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• 2011

Purpose: The purpose of this study was to measure the angular differences between the horizontal reference planes on the CBCT generated PA cephalogram and the modified interpupillary plane, which was usually used in the clinical examination, and to evaluate the validity of the horizontal reference planes. Methods: The CBCT generated PA cephalogram was used to measure the angles between the FH, Lo and IP planes. The subjects consisted of 42 patients with facial asymmetry (males: 21, females: 21, mean-age: 21.6 years). The control groups were also assessed (males: 10, females: 10, mean-age: 23.8 years). The distance of the interpupil was measured on the soft-tissue volume rendered image. The angular differences were statistically analyzed using the $Mann-Whitney$ $U$ $test$ for inter-group comparisons and the $Friedman$ $test$ for intra-group comparisions. Results: The angle between the FH plane and IP plane (the angle of the FH-IP line) showed a statistically significant difference between the two groups ($p$ <0.05). There was no statistical differences between each angle (angle of the FH-IP line, angle of the FH-Lo line, angle of the Lo-IP line) on the intra-group comparision ($p$ >0.05). Conclusion: The angle between the Lo line and IP line (angle of the Lo-IP line) showed no statistically significant difference in both the control and asymmetry groups. Therefore, the Lo line could be used as a horizontal reference plane in CBCT generated PA cephalograms.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.4
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• pp.589-594
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• 2002

The filter of hairpin type can be designed small but has a demerit which a design method is complex. This paper suggests the method that a hairpin line filter is designed with applying a transmission line method and J inverter circuit, accurately calculated the J value and the b parameter. It is considered about the line have no the mutual coupling element with next line to each other. is calculated a length of electric coupling line about coupling element in each inverter stage from the J value and the b parameter. In order to show the validity of the proposed design method, band pass filter of small hairpin line is designed at center frequency 2.6 GHz, with Serenade 8.0 software tool and the simulation characteristics are compared with design results.

• Journal of the Korean Society of Costume
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• v.45
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• pp.17-28
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• 1999

This study was to analyze factors of the body form and factors of bodice pattern through a surface developement figure for the tight bodice pattern design. Fifty replicas of the trunk surface were made by applying a surgical tape method of female subjects between 18 and 24 years old. The result was as follows: 1. The gaps between direct measurements and measurements of the surface development figure were regarded as allowances for the tight bodice pattern. 2. There are four factors of body form selected by factor analysis. The 1st factor signifies th degree of fatness in the upper body. The 2nd factor signifies the length of the upper body. The 3rd factor signifies the part of the front shoulder. The 4th factor signifies the part of the neck. 3. There are high correlations between center back line of the surface development figure and back length line side line front length line back neck depth back upper chest depth. There are high correlations between back bust line of the surface development figure and back interscye breadth line back upper chest line back armhole line. There are high correlations between front bust line of the surface development figure and front upper chest line front interscye breadth line front armhole line front neck breadth 4. The regression expressions of measurements of the surface development figure of the upper body were analyzed as(Fig. 3).

• Proceedings of the KIEE Conference
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• 2002.07b
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• pp.952-954
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• 2002

The effort for measuring the rotor vibration caused by the short of rotor winding or air-gap eccentricity has been carried on. This paper introduces on-line vibration monitoring technologies using search coils to measure flux for synchronous generators. A 3,300/6,600 (V), 150 [kW] synchronous generator is built, and search coils are designed and inserted in the wedges. Furthermore, air-gap eccentricity is analyzed by finite element method and compared with experiment tests. Induced voltages caused by different air-gap eccentricity on the search coils are compared as well.

• Proceedings of the KIEE Conference
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• 2004.10a
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• pp.37-39
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• 2004

This study investigates the characteristic analysis of Line-start Permanent Magnet Synchronous Motor considering PWM Inverter. The effects of the PWM inverter on machine characteristic are analyzed by using direct co-simulation of Matlab Simulink and Flux2D. Validity of analysis method is confirmed by the analysis result of Sin-wave power Model.

• 한국정보디스플레이학회:학술대회논문집
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• 2008.10a
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• pp.1573-1576
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• 2008

A simulation system is developed to calculate the apparent motion-induced image from a sequence of temporal luminance transitions, while using the properties of the human visual system. Based on the simulation method, both edge (moving block) and detail degradation (line spreading, grating, sinusoidal pattern), and also color aberration are discussed.