• Asian-Australasian Journal of Animal Sciences
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• 제17권12호
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• pp.1657-1662
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• 2004

The forage potential of mulberry (Morus alba) was evaluated under Malaysian conditions. The yield and nutrient composition of plant fractions of mulberry (whole plant, leaf and stem fractions) were determined at four harvest stages, namely, 3 (W3), 5 (W5), 7 (W7) and 9 (W9) weeks in a randomized block design. The study was conducted over a 9-month period to assess the persistence of the crop to repeated harvests. Fresh and dry matter (DM) yields of all plant fractions increased significantly (p<0.01) with increasing intervals between harvests, with highest DM yields at W9. The leaf to stem ratio declined significantly (p<0.01) from 5.2 (W3) to 0.9 (W9), indicating predominance of the stem fraction with advancing maturity. The nutritional composition of plant fractions was also significantly influenced (p<0.01) by advancing plant maturity at harvest. Crude protein (CP), ash and the metabolisable energy content of plant fractions declined significantly (p<0.01) from W3 to W9, while there was a corresponding significant increase (p<0.01) in the acid detergent fibre, neutral detergent fibre and acid detergent lignin. From this study it was concluded that the optimum stage to harvest the whole plant is 5 weeks, which is a compromise between yield, nutrient composition (CP and fibre components), and the annual number of cuts, with good crop persistence to repeated harvests. Fresh mulberry whole plant can provide a valuable supplemental source of nutrients to poor quality basal diets.

• Asian-Australasian Journal of Animal Sciences
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• 제9권2호
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• pp.203-210
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• 1996

A 13-month study was conducted to determine the monthly variation of crude protein(CP), cell contents(CC), fiber fractions and mineral concentrations of paragrass [Brachiaria mutica (Forsk.) Stapf.] and stargrass (Cynodon plectostachyum Pilger) and to estimate the correlations among the nutrient fractions and climatic factors. The forage samples collected by hand plucking were found to contain CP and mineral concentrations. i.e. calcium(Ca), magnesium(Mg), potassium(K) and zinc(Zn) above the critical levels based on ruminant veeds. Monthly differences(p<0.05) were observed in all CP, CC, neutral detergent fiber(NDF), acid detergent fiber(ADF), hemicellulose(HE), cellulose(CE) and acid detergent lignin(ADL) between the two grass species. Monthly differences(p<0.05) were also observed in all concentrations of forage Ca, K, phosphorus(P), copper(Cu) and Zn except in Mg of both grasses and K of stargrass. Species differences(p<0.05) were observed in all nutrient fractions except Mg and K concentrations. Rainfall had positive correlations to CP, P(p<0.01) and CC(p<0.05); it had negative correlations to NDF(p<0.05). ADF, CE, Ca, Cu and Zn(p<0.01). Temperature, humidity and daylength had also some correlations to various nutrient fractions.

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.680-692
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• 2010

This paper gives an overview of the availability, nutritive quality, and possible strategies to improve the utilization of rice straw as a feed ingredient for ruminants. Approximately 80% of the rice in the world is grown by small-scale farmers in developing countries, including South East Asia. The large amount of rice straw as a by-product of the rice production is mainly used as a source of feed for ruminant livestock. Rice straw is rich in polysaccharides and has a high lignin and silica content, limiting voluntary intake and reducing degradability by ruminal microorganisms. Several methods to improve the utilization of rice straw by ruminants have been investigated in the past. However, some physical treatments are not practical because of the requirement for machinery or treatments are not economical feasible for the farmers. Chemical treatments, such as NaOH, $NH_3$ or urea, currently seem to be more practical for onfarm use. Alternative treatments to improve the nutritive value of rice straw are the use of ligninolytic fungi (white-rot fungi), with their extracellular ligninolytic enzymes, or specific enzymes degrading cellulose and/or hemicellulose. The use of fungi or enzyme treatments is expected to be a more practical and environmental-friendly approach for enhancing the nutritive value of rice straw and can be costeffective in the future. Using fungi and enzymes might be combined with the more classical chemical or physical treatments. However, available data on using fungi and enzymes for improving the quality of rice straw are relatively scarce.

• Journal of the Korean Wood Science and Technology
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• 제42권6호
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• pp.700-707
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• 2014

본 연구에서는 고온증기 및 오존처리 전처리 후, 습식 고전단 해섬하여 국내산 잣나무로부터 리그노셀룰로오스 나노섬유를 제조하였다. 고온증기 및 오존 전처리에 의해 헤미셀룰로오스 및 리그닌 성분은 각각 약 40%, 42%의 감소효과를 보였으며, 무처리 목분에 비교하여 습식 고전단 해섬처리 시간이 증가함에 따라 섬유의 직경이 더욱 크게 감소하였으며, 좁은 치수분포를 나타냈다. 두 전처리 후 얻어진 리그노셀룰로오스 나노섬유는 평균직경이 각각 19 nm 및 12 nm로 매우 가느다란 것으로 관찰되었다. 얻어진 리그노셀룰로오스 나노섬유를 폴리우레탄 폴리머의 강화필러로 첨가한 결과, 첨가량 및 해섬처리 시간이 증가함에 따라 복합재료의 인장강도와 탄성율이 증가하였다. 특히, 두 전처리 후 얻어진 리그노셀룰로오스 나노섬유의 경우가 전처리하지 않은 경우에 비해 복합재료의 인장강도 특성을 더 향상시키는 것을 알 수 있었다.

• 신재생에너지
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• 제6권4호
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• pp.6-14
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• 2010

Pretreatment of cellulosic biomass is necessary before enzymatic saccharification and fermentation. Extrusion is a well established process in food industries and it can be used as a physicochemical treatment method for cellulosic biomass. Aqueous ammonia soaking treatment at mild temperatures ranging from 60 to $80^{\circ}C$ for longer reaction times has been used to preserve most of the cellulose and hemicellulose in the biomass. The objective of this study was to evaluate the effect of extrusion treatment on aqueous ammonia soaking method. Extrusion was performed with miscanthus sample conditioned to 2mm of particle size and 20% of moisture content at $200^{\circ}C$ of barrel temperature and 175rpm of screw speed. And then aqueous ammonia soaking was performed with 15%(w/w) ammonia solution at $60^{\circ}C$ for 1, 2, 4, 8, 12 hours on the extruded and raw miscanthus samples respectively. In the combined extrusion-soaking treatment, most compositions removal occurred within 1~2 hours and on a basis of 1 hour soaking treatment values, cellulose was recovered about 85% and other compositions, including hemicellulose, are removed about 50% from extruded miscanthus sample. The combined extrusion-soaking treated and soaking only treated samples were subjected to enzymatic hydrolysis using cellulase and ${\beta}$-glucosidase. The enzymatic digestibility value of combined extrusion-2 hours soaking treated sample was comparable to 12 hours soaking only treated sample. It means that extrusion treatment can shorten the conventional long reaction time of aqueous ammonia soaking. The findings suggest that the combination of extrusion and soaking is a promising pretreatment method to solve both problems for no lignin removal of extrusion and long reaction time of aqueous ammonia soaking.

• Asian-Australasian Journal of Animal Sciences
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• 제33권8호
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• pp.1273-1283
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• 2020

Objective: This study investigated a method of preparing corn stover for Irpex lacteus (I. lacteus) treatment to improve its in vitro rumen degradability under non-sterile conditions. Methods: Corn stover was inoculated with Lactobacillus plantarum (L. plantarum), Lactobacillus buchneri (L. buchneri), and an equal mixture of these strains, and ensiled for 0, 3, 7, 14, and 28 days. After each period, a portion of the silage was sampled to assess the silage quality, and another portion of the silage was further treated with I. lacteus at 28℃ for 28 d. All the samples were analyzed for fermentation quality, chemical composition, and in vitro gas production (IVGP) as a measure of rumen fermentation capacity. Results: Lactic acid bacteria (LAB) was found to improve the silage quality of the corn stover, and the corn stover silage inoculated with L. plantarum produced more lactic acid and higher IVGP than other silage groups. The I. lacteus colonies flourished in the early stage of corn stover silage, especially on the 3-d corn stover silage inoculated with both L. plantarum and L. buchneri. This led to an 18% decrease in the acid detergent lignin content, and a 49.6% increase in IVGP compared with the raw stover. Conclusion: The combination of ensiling with the mixed LAB inoculation and I. lacteus treatment provided a cost-effective method for the improvement of the IVGP of corn stover from 164.8 mL/g organic matter (OM) to 246.6 mL/g OM.

• 펄프종이기술
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• 제33권3호
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• pp.75-83
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• 2001

The potential of oxygen delignification is a powerful tool to reduce detrimental environmental effects. This study was performed to investigate the effect of steam explosion treatment of chemically treated oak wood on oxygen-alkali pulping. Pulp yield during steam explosion treatment by ${Na_2}{O_3}$-NaOH impregnation was higher than the other impregnation chemicals. Also, NaOH extraction at room temperature after steam explosion treatment improved the kappa number from 140~116 to 90~64. Oxygen-alkali pulping of chemical steam explosion treated woods affected to pulp yields. ${Na_2}{O_3}$-NaOH impregnation was very effective to higher carbohydrate yields at same delignification level. Its carbohydrate yield seemed to be highly related to the effluent pH. Oxygen-alkali pulping after steam explosion treatment of ${Na_2}{O_3}$-NaOH impregnated wood was shown that carbohydrate yield was very high because its effluent pH was increase from natural to mild alkali. Even if oxygen bleaching limit the delignification to 50% in order to avoid unacceptable yield and viscosity losses, oxygen-alkali pulping after steam explosion by ${Na_2}{O_3}$-NaOH impregnation was possible to extend the delignification more than 80%. Considering high pulp yield with lower lignin content from steam explosion treated wood, it might be profitable to end the cook at a high kappa number instead of a low kappa number, and continuously apply the oxygen delignification, in order to better quality pulp.

• 한국초지조사료학회지
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• 제34권1호
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• pp.15-20
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• 2014

To investigate lignification process and its physiological significance under water-deficit condition, the responses of peroxidases, polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) in relation to leaf water status to the short term of water deficit treatment in the leaves with different maturities in forage rape were measured. The significant decrease in relative water content (RWC) and leaf osmotic potential (${\Psi}{\pi}$) were apparent after 5 d of water-deficit treatment. The activity of guaiacol peroxidase (GPOD), ascorbate peroxidase (APOD), coniferyl alcohol peroxidase (CPOD), and syringaldazine peroxidase (SPOD) was depressed especially in middle and old leaves when compared with that of control leaves. On the other hand, in young leaves, a significant increase in CPOD (+34%) and SPOD (+24%) activity as affected by water-deficit treatment was apparent. The activation of PAL and PPO was observed in middle and old leaves for PAL and in young and middle leaves for PPO. These results suggest that peroxidases in middle and old leaves did not involve in lignification under mild water-deficit stress, whereas CPOD and SPOD in young leaves participate in lignification by a coordination with PAL and PPO to incorporate phenol and lignin into the cell walls.

• 한국식품영양과학회지
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• 제33권5호
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• pp.852-856
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• 2004

쌈샐러드 11종류의 일반성 분과 구조탄수화물인 식이섬유류(TDF, IDF, SDF, NDF, DFi)를 측정하였다 쌈샐러드의 조단백질과 조지방 함량이 2∼3배 높았으며 조회분의 경우 곡류보다 7배 가량 높았다. 전조물 기준으로 TDF 함유량에 있어서 Costamary가 32.61%로 낮았으나 Treviso가 41.22%로 높았으며, IDF는 Red leaf beet이 21.58%로 낮았고, Treviso가 28.95%로 높았으나 SDF는 Nakai이 6.60%로 낮았고 Common danelion이 14.70%로 매우 높았다. 건물기준으로 total carbohyate는 Salad bowl이 73.65%로 가장 높았고 Red leaf beet이 36.30%로 가장 낮았고, NDF는 Nakai가 48.83%로 가장 높았고 Red leaf beet이 29.60%로 가장 낮았다 ADL은 Salad bowl이 27.65%로 높았고 가장 낮은 것은 Com salad로 2.92%였으며 hemicellulose는 Nakai가 22.55%로 가장 높았고 Salad bowl이 2.15%로 낮았다.

• Journal of the Korean Wood Science and Technology
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• 제46권3호
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• pp.285-296
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• 2018

ISPM No. 15에 의거한 열처리 목재의 검증을 위한 연구로서 열처리 목재 표면의 분광학적 특성을 분석하였다. IR 스펙트럼 상에서는 다양한 작용기들이 확인되지만 수종간, 열처리 시간 및 보관 기간에 따른 특별한 차이를 발견하기 어려웠다. HBI(hydrogen-bonding intensity)는 보관 기간에 따른 열처리 목재의 변화는 관찰할 수 있지만 열처리 시간에 따른 변화는 관찰하기 어려웠다. 하지만 PCA score plot 상에서 수종 내에서 60분 혹은 90분의 열처리 시간에 따라 목재의 분류가 가능하였다. PCA에서 열처리 목재의 분류의 기준은 목재 내 리그닌의 방향족 환과 셀룰로오스의 C-H bending이었으며 이들 성분에 의해 ISPM No. 15로 열처리된 목재를 분류할 수 있었다.