• 대한수의학회지
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• 제39권5호
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• pp.945-950
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• 1999

A 4-year-old female American cocker spaniel was hospitalized with 6 months prolonged dermal problems in The Veterinary Medical Teaching Hospital of Seoul National University. Initial noticed signs by owner of this dog were rashes and papules on ear part and they were spreaded whole body. On initial physical examinations, papules, pustules, crusts, and erythema were presented on whole body. Diameters of focal scaly and erythematous alopetic lesions were varied ranging 1 to 4cm. Skin scraping for detecting ectoparasites and wood's light examinations for dermatophyte were negative. Any bacteria were not detected but Malassezia pachydermatis was detected on cytological examination and fungal culture. The dog was treated with Itraconazole (5mg/kg, q12h) and Cephalexin (20mg/kg, q12h) orally and then bathed with 2% chlorhexidine shampoo twice per week for 5 weeks. From 12 weeks after starting therapy, all dermal problems were disappeared although some mild pyoderma were once relapsed. Regular chlorhexidine bathing treatment so far has been continued for preventing recurrence.

• 정신신체의학
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• 제15권1호
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• pp.14-21
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• 2007

월경전 불쾌기분 장애(premenstrual dysphoric disorder, PMDD)는 여성의 약 $4{\sim}5%$에서 발병하며 섹스 스테로이드 호르몬 양의 변화에 대한 증가된 반응성 및 세로토닌, 멜라토닌, GABA의 감소가 원인으로 생각된다. 우울증, 계절성 정동 장애, 공황장애, 신경성 폭식증은 질병 특성 상 공통점이 있어서 월경전 불쾌기분 장애와 상호 연관성을 암시한다. DSM-IV-TR의 진단 기준에 따라 월경전 불쾌기분 장애를 진단하려면 반드시 정신적 증상이었어야 한다. 흔한 정신적 증상은 이자극성, 분노, 우울, 기분 스윙, 정동 불안정, 긴장, 불안, 피로, 음식 갈망이다. 현재로서 최선의 치료 약물은 세로토닌 재흡수 차단제이며, leuprolide, danazol, estradiol, spironolactone, bromocriptine도 사용될 수 있다. 경증 및 중증도 환자에 대한 보조적 치료로는 식이요법, 운동, 광선요법, 정신치료, 일기 쓰기 등이 있다.

• PNF and Movement
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• 제4권1호
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• pp.51-62
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• 2006

Purpose : The purposes of this study were to test the effect of proprioceptive and vestibular sensory input on expression of BDNF after traumatic brain injury in the rat. Subject : The control group was sacrificed at 24 hours after traumatic brain injury. The experimental group I was housed in standard cage for 7 days. The experimental group II was housed in standard cage after intervention to proprioceptive and vestibular sensory(balance training) for 7 days. Method : Traumatic brain injury was induced by weight drop model and after operation they were housed in individual standard cages for 24 hours. After 7th day, rats were sacrificed and cryostat coronal sections were processed individual1y in goat polyclonal anti-BDNF antibody. The morphologic characteristics and the BDNF expression were investigated in injured hemisphere section and contralateral brain section from immunohistochemistry using light microscope. Result : The results of this experiment were as follows: 1. In control group, cell bodies in lateral nucleus of cerebellum, superior vestibular nucleus, purkinje cell layer of cerebellum and pontine nucleus changed morphologically. 2. The expression of BDNF in contralateral hemisphere of group II were revealed. 3. On 7th day after operation, immunohistochemical response of BDNF in lateral nucleus, superior vestibular nucleus, purkinje cell layer and pontine nucleus appeared in group II. Conclusion : The present results revealed that intervention to proprioceptive and vestibular sensory input is enhance expression of BDNF and it is useful in neuronal reorganization improvement after traumatic brain injury.

• PNF and Movement
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• 제5권1호
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• pp.9-17
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• 2007

Purpose : The purpose of this study was to test the effect of balance training for proprioceptive and vestibular sensory stimulation and therapeutic environment on expression of BDNF after traumatic brain injury in the rat. Subject : Twelve Sprague-Dawley rats were randomly assigned into group I and group II. After traumatic brain injury, group I was housed in standard cage for 7 days. Group II was housed in therapeutic cage after balance training for 7 days. Method : Traumatic brain injury was induced by weight drop model and after operation they were housed in individual standard cages for 24 hours. After 7th day, the rats were sacrificed and cryostat coronal sections were processed individually in goat polyclonal anti-BDNF antibody. The morphologic characteristics and the BDNF expression were investigated in injured hemisphere section from immunohistochemistry using light microscope. Result : Immunohistochemical response of BDNF in lateral nucleus, purkinje cell layer, superior vestibular nucleus and pontine nucleus appeared very higher in group II than in group I Conclusion : The present result revealed that simultaneously application of balance training for proprioceptive and vestibular sensory stimulation input and therapeutic environment in traumatic brain injured rats is enhance expression of BDNF and it is facilitates neural plasticity.

• 약학회지
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• 제54권2호
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• pp.112-121
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• 2010

The cytotoxic activity of 33,4,5-trihydroxybenzoic acid methylester and related compounds on the growth of normal cell lines, human skin melanoma cells and human oral epithelioid cell line were evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis-[2-methoxy-4-nitro-5-sulfo-phenyl]-2-H-tetrazolium-5-caboxanilide (XTT) methods. 3,4,5-Trihydroxybenzoic acid methylester decreased the cell viability of human skin melanoma cells and human oral epithelioid cells shown by the MTT method and the cell adhesion activity of human skin melanoma cells and human oral epithelioid cells shown by the XTT method. In light microscopy, 100 ${\mu}M$ 3,4,5-trihydroxybenzoic acid methylester showed the highest cytotoxic activity. These results suggest that 3,4,5-trihydroxybenzoic acid methylester has a potential anticancer activity.

• 대한예방한의학회지
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• 제13권2호
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• pp.39-50
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• 2009

Objectives : In order to evaluate the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds on the growth of normal cell lines and human oral epithelioid cell line, cell viability, cell adhesion ability, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide-[MTT), and 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium -5-caboxanilide-[XTT) methods. Results : The cytotoxicity of 3,4,5-trihydroxybenzoic acid($IC_{50}$, $2,552.40\;{\mu}M$) was low according to the toxic criteria. Cytotoxic effect of 3,4,5-trihydroxybenzoic acid and related compounds against $IC_{50}$ value in cell morphology increased in a concentration-dependent manner. In light microscopy, $100\;{\mu}M$ 3,4,5-trihydroxy-benzoic acid showed th highest cytotoxic activity. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may have a potential anticancer activity.

• 대한의료기공학회지
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• 제5권1호
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• pp.148-159
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• 2001

The Spreading-Qi recorded curative effect with a long history. I studied the Qi-therapy through train of the Qi-sender. methods of the Spreading-Qi and clinical application of the Spreading-Qi. 1. Train of the Qi-sender. Sitting, standing and ling postures can all be applied as training postures for training Qi. Qi grows in Lower Dan(elixir field) and circulates in heavenly circuits by static Qi. Training Qi by dynamic Qi is a fundamental exercise for the maneuvers to conduct Qi. 2. Methods of the Spreading-Qi, Breathe naturally concentrate the mind on Lower Dan(elixir field). When exhaling. Mindwill accompanies Qi to go to the Conception Vessel(CV) and Governor Vessel(GV), conduct Qi to the palms or fingers and emit Qi, with the emitting site touching or leaving the treated region. 3. Clinical application of the Spreading-Qi, its wide range of indications. 4. The Spreading-Qi is similar to Western 'Healing' and 'Hands of light'. Mind will accompanies energy to go to hand and emit energy to the treated region.

• Archives of Plastic Surgery
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• 제34권4호
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• pp.426-431
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• 2007

Purpose: The aim of this study is to compare the effects of bone marrow stromal cells(BSCs) and fibroblasts on wound healing activity in vivo, especially on epithelization. Methods: The fibroblasts and BSCs were harvested from patients and cultured. Ten Spague-Dawley white rats were used. A 5 mm punches were made to excise skin and subcutaneous tissue in a round fashion at six sites on the back area of each rat. Four hundred thousand cells suspended in 0.05 ml fibrinogen were applied to the created wounds. The cells in group I, II, and III were no cells, fibroblasts and BSCs. The lengths of epithelial gap at the widest wound site were compared with autopsy specimens obtained on the 6th day after cell therapy under light microscope. Statistical comparisons were performed using the Mann-Whitney U-test, and the p value < 0.05 was considered statistically significant. Results: The best epithelization was also seen in the BSC group, followed by fibroblast and no cell groups.Conclusion: These results demonstrate that BSC has superior effect on stimulating wound healing than fibroblast, which is currently used for wound healing.

• 대한후두음성언어의학회지
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• 제31권2호
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• pp.92-95
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• 2020

Tracheal granuloma, the most commonly reported sequela of pediatric tracheotomy. A variety of techniques are available for the management of tracheal granuloma. Potassium-titanyl-phosphate (KTP) laser has been previously established as an acceptable technique for removal of laryngeal surgery, which emits a green light with a wave length of 532 nm, which is well-absorbed by hemoglobin and can coagulate and vaporize tissue. The ability to deliver laser energy through a flexible glass fiber makes the technique convenient for use with a rigid bronchoscope, overcoming problems with intraluminal access encountered with earlier attempts at CO2 laser therapy for this problem. Another advantage of KTP laser is the avoidance of the risks and morbidity associated with an open procedure. We report our surgical technique KTP laser in the management of tracheal granuloma removal into the tracheostomy site. KTP laser is good tool for management of tracheal granuloma with low incidence of complications.

• 청정기술
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• 제26권4호
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• pp.251-256
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• 2020

The biocompatibility and plasmonic properties of Au nanoparticles make them useful for photothermal therapy, drug delivery, imaging, and many other fields. This study demonstrated a novel, facile, economic, and green synthetic method to produce gold nanoparticles. Gold nanoparticles (AuNPs) with spherical and triangular shapes were effectively synthesized using only Schisandra chenesis fruit extract as the capping and reducing agent. The shape of the AuNPs could be engineered simply by adjusting the molar concentration of HAuCl4 in the reaction mixture. The as-synthesized AuNPs were characterized using UV-VIS spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and energy dispersive X-ray analysis (EDXA). This study revealed that by using the HAuCl4 concentration in the AuNP synthesis, the shape and size of the AuNPs could be controlled by the concentration of HAuCl4 and Schisandra chinensis fruit extract as a surfactant. The as-synthesized AuNPs samples had sufficient colloidal stability without noticeable aggregation and showed the predominant growth of the (111) plane of face-centered cubic gold during the crystal growth. The catalytic efficiency of the AuNPs synthesized using Schisandra chenesis fruit extract was examined by monitoring the catalytic reduction of 4-nitrophenol to 4-aminophenol using Ultraviolet-visible spectroscopy (UV-Vis spectroscopy). The synthesized AuNPs showed good catalytic activity to reduce 4-nitrophenol to 4-aminophenol, revealing their practical usefulness.