• Title/Summary/Keyword: light penetration depth

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Pigment Reduction to Improve Photosynthetic Productivity of Rhodobacter sphaeroides

  • Kim, Nag-Jong;Lee, Jeong-Kug;Lee, Choul-Gyun
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.442-449
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    • 2004
  • Improving the light utilization efficiency of photosynthetic cells in photobioreactors (PBRs) is a major topic in algal biotechnology. Accordingly, in the current study we investigated the effect and suitability of photosynthetic pigment reduction for improving light utilization efficiency. The light-harvesting complex II (LH-II) genes of Rhodobacter sphaeroides were removed to construct a mutant strain with less pigment content. The mutant strain exhibited a slower growth rate than the wild-type under a low light intensity, while the mutant grew faster under a high light intensity. In addition, the specific absorption coefficient was lower in the mutant due to its reduced pigment content, thus it seemed that light penetrated deeper into its culture broth. However, the distance (light penetration depth) from the surface of the PBR to the compensation point did not increase, due to an increase in the compensation irradiance of the mutant strain. Experimental data showed that a reduced photosynthetic pigment content, which lessened the photoinhibition under high-intensity light, helped the volumetric productivity of photosynthetic microorganisms.

NEAR INFRARED SPECTROSCOPY, A POWERFUL TECHNIQUE IN HUMAN SKIN STUDY : PART I METHOD RELIABILITY AND INFLUENTIAL PARAMETERS

  • Snieder, Marchel;Wiedemann, Sophie;Hansen, Wei G.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.3101-3101
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    • 2001
  • Near Infrared spectroscopy (NIR) used on human skin measurement was explored in the past decade. Many publications in different journals and magazines discussed the feasibility of the NIR technique for cosmetic product property studies. Based upon the results of pioneers, we have pursued some work of the NIR instrument coupled with a probe module for skin measurement in vivo and vitro. In part I of this paper, the specific Near Infrared spectroscopy instrument stability, human subject conditions and other parameters, which could affect the measurements reproducibility are discussed. Second derivative NIR spectra and Principle Components Analysis (PCA) are utilised for data interpretation. In part II of this paper, the relationship of human skin moisture and ageing, the gender information and finally, the discovery of penetration depth of NIR incident light on skin are reported. A theoretical penetration depth calculation equation is proposed. In part III, the study results of a couple of commercial skin care products effect will be described. The skin lotions were applied on human skin (in vivo) in order to exam the NIR feasibility to monitor the changes of moisture level. The results are consistently positive. From our primary study, it can conclude that the NIR is potentially a very powerful instrument for skin condition diagnostics, either for cosmetic and/or for medication purposes.

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Measurement of Optical Parameters of Biological Tissues by Using the Frequency-Domain Spectroscopy (주파수 영역 분광법을 이용한 생체의 광학계수 측정법 연구)

  • Jeon, K.J.;Yoon, G.;Kim, H.S.;Kim, W.K.;Yi, J.H.;Park, S.H.;Kim, U.
    • Proceedings of the KOSOMBE Conference
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    • v.1997 no.05
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    • pp.379-382
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    • 1997
  • A method for measuring optical properties of biological medium was investigated by using the frequency domain spectroscopy. When amplitude-modulated light with the frequency of several tens up to several hundred MHz propagates through a scattering medium, the phase lag and the amplitude reduction occur. The phase lag depends on the average of optical path lengths. The amplitude reduction with respect to the radial distance is influenced by the penetration depth. The mean of optical path length and penetration depth are related to optical coefficients. The phase lag and the amplitude reduction were measured based on the heterodyne detection method. The experimental data were fitted with the theoretical curves derived from diffusion theory and the absorption and scattering coefficients were calculated.

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Full-scale TBM excavation tests for rock-like materials with different uniaxial compressive strength

  • Gi-Jun Lee;Hee-Hwan Ryu;Gye-Chun Cho;Tae-Hyuk Kwon
    • Geomechanics and Engineering
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    • v.35 no.5
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    • pp.487-497
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    • 2023
  • Penetration rate (PR) and penetration depth (Pe) are crucial parameters for estimating the cost and time required in tunnel construction using tunnel boring machines (TBMs). This study focuses on investigating the impact of rock strength on PR and Pe through full-scale experiments. By conducting controlled tests on rock-like specimens, the study aims to understand the contributions of various ground parameters and machine-operating conditions to TBM excavation performance. An earth pressure balanced (EPB) TBM with a sectional diameter of 3.54 m was utilized in the experiments. The TBM excavated rocklike specimens with varying uniaxial compressive strength (UCS), while the thrust and cutterhead rotational speed were controlled. The results highlight the significance of the interplay between thrust, cutterhead speed, and rock strength (UCS) in determining Pe. In high UCS conditions exceeding 70 MPa, thrust plays a vital role in enhancing Pe as hard rock requires a greater thrust force for excavation. Conversely, in medium-to-low UCS conditions less than 50 MPa, thrust has a weak relationship with Pe, and Pe becomes directly proportional to the cutterhead rotational speed. Furthermore, a strong correlation was observed between Pe and cutterhead torque with a determination coefficient of 0.84. Based on these findings, a predictive model for Pe is proposed, incorporating thrust, TBM diameter, number of disc cutters, and UCS. This model offers a practical tool for estimating Pe in different excavation scenarios. The study presents unprecedented full-scale TBM excavation results, with well-controlled experiments, shedding light on the interplay between rock strength, TBM operational variables, and excavation performance. These insights are valuable for optimizing TBM excavation in grounds with varying strengths and operational conditions.

Development of Light weight Aluminum Subframe using Hybrid forming process (복합성형공법 적용 경량 알루미늄 서브프레임 개발)

  • Kwon, T.W.;Park, B.C.;Jang, G.W.;Lee, W.S.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2007.05a
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    • pp.361-363
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    • 2007
  • The light weight aluminum subframe for automobile chassis part was developed using hybrid process, i.e. extruforming, press stamping and MIG welding. To achieve a 30 % weight reduction compared with conventional steel subframe keeping satisfactory performance, the design of cross-section of extruforming part was introduced, then forming simulation was performed and the final design was determined. In addition, we tried to estibilish optimun aluminum welding conditions for good penetration depth and few pore defact, finally the prototype of aluminum subframe was assembled using MIG welding method.

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Penetration depth and Wave Propagation in Random Media (무질서한 매질에서 침투깊이와 파동 전파)

  • Kim, Ki-Jun;Sung, Ki-Chun
    • Journal of the Korean Applied Science and Technology
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    • v.23 no.1
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    • pp.70-76
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    • 2006
  • The influence of fluorophor, scatterer, absorber in turbid materials by light scattering were interpreted for the scattered fluorescence intensity and wavelength, it is studied the molecular property by laser induced fluorescence spectroscopy. It can be found that the effects of optical property are penentrated in scattering media by the optical $parameters({\mu}s$, ${\mu}a$, ${\mu}t$, ${\gamma}$, ${\rho})$. The value of scattering coefficient ${\mu}s$ is large appeared by means of the increasing particles of scattering, it can be found that the slope appears exponentially as a function of distance from laser source to detector. It may also utilize in designing the best model for oil chemistry, laser medicine and application of medical engineering.

A Study on the Measurements of Optical Parameters in Photosensitizer by Light Scattering (농도가 진한 매질에서 광증감제에 의한 광학적 파라미터측정에 관한 연구)

  • Kim, Ki-Jun;Lee, Jou-Joub
    • Journal of the Korean Applied Science and Technology
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    • v.28 no.1
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    • pp.102-108
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    • 2011
  • The study of wave propagation and scattering in biological media has become increasingly important in recent years. The propagation of light within tissues is an important problem that confronts the dosimetry of therapeutic laser delivery and the development of diagnostic spectroscopy. In the clinical application of photodynamic therapy(PDT) and in photobiology, the photon deposition within a tissue determines the spatial distribution of photochemical reactions. Scattered light is measured as a function of the distance (r) between the axis of the incident beam and the detection spot. Consequently, knowledge of the photosensitizer(Chlorophyll-a) function that characterizes a phantom is measured. To obtain the results of scattering coefficients(${\mu}s$) of a turbid material from diffusion described by experimental approach. It was measured the energy fluency of photon radiation at the position of penetration depth. From fluorescence experimental method obtained the analytical expression for the scattered light as the values of $(I/I_o)_{wavelength}$ vs the distance between the center of the incident beam and optical fiber in terms of the condition of "in situ spectroscopy(optically thick)" and real time by fluorometric measurements. The result was compromised with transport of intensities though a random distribution of scatters.

A Study on the Optical Influence by Photosensitizer in Vitro (In Vitro에서 광증감제에 의한 광학적 영향에 관한 연구)

  • Kim, Ki-Jun;Sung, Ki-Chun
    • Journal of the Korean Applied Science and Technology
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    • v.22 no.2
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    • pp.182-190
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    • 2005
  • The propagation of light radiation within tissues is an important problem that confronts the dosimetry of therapeutic laser delivery and the development of diagnostic spectroscopy. In the clinical application of photodynamic therapy(PDT) and in photobiology, the photon deposition within a tissue determines the spatial distribution of photochemical reactions. Scattered light is measured as a function of the distance (r) between the axis of the incident beam and the detection spot. Consequently, knowledge of the photosensitizer(Chlorophyll-a) function that characterizes a phantom is important. To obtain the results of scattering coefficients(${\mu}s$) of a turbid material from diffusion described by experimental approach. It was measured the energy fluency of photon radiation at the position of penetration depth. From fluorescence experimental method obtained the analytical expression for the scattered light as the values of $(I\;/I_o)_{wavelength}$ vs the distance between the center of the incident beam and optical fiber in terms of the condition of "in situ spectroscopy(optically thick)" and real time by fluorometric measurements.

A Cost-effective Light Emitting Diode-acoustic System for Preclinical Ocular Applications

  • Choi, Hojong;Ryu, Jaemyung;Yeom, Jung-Yeol
    • Current Optics and Photonics
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    • v.2 no.1
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    • pp.59-68
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    • 2018
  • Opto-acoustic systems provide structural and functional information regarding biological tissues. Conventional opto-acoustic systems typically employ continuous or pulsed lasers as transmission sources. Compared to lasers, light emitting diodes (LEDs) are cost-effective and relatively portable excitation sources but are non, coherent. Therefore, in this study, a relatively low cost lens - a type of Ramsden eyepiece - was specially designed to theoretically calculate the illumination and achieve a constant brightness across the pupil of an eye. In order to verify the capability of the developed light-emitting diode-acoustic (LEDA) systems, we carried out experiments on bovine and bigeye tuna eyeball samples, which are of similar size to the human eye, using low frequency (10 MHz) and high frequency (25 MHz) ultrasound transducers. High frequency ultrasound transducers are able to provide higher spatial resolution compared to low frequency ultrasound transducers at the expense of penetration depth. Using the 10 MHz and 25 MHz ultrasound transducers, acceptable echo signals (3.82, 3.94, and 5.84 mV at 10 MHz and 282, 1557, 2356 mV at 25 MHz) from depth greater than 3 cm and 6 cm from the anterior surface of the eye were obtained. We thereby confirmed that the LEDA system using a pulsed LED with the designed Ramsden eyepiece lens, used in conjunction with low and high frequency ultrasound transducers, has the potential to be a cost-effective alternative method, while providing adequate acoustic signals from bovine and bigeye tuna ocular areas.

Effect of Configuration of Shaft and Helix Plate on Bearing Capacity of Moderate-size Helical Pile : I. Test-bed Construction and Field Loading Test (중소구경 헬리컬 파일의 축과 원판의 형상이 지지력에 미치는 영향 평가 : I. 시험시공과 현장재하시험)

  • Lee, Jongwon;Lee, Dongseop;Kim, Hyung-Nam;Choi, Hangseok
    • Journal of the Korean Geosynthetics Society
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    • v.13 no.2
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    • pp.31-39
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    • 2014
  • The helical pile is a manufactured steel pile consisting of one or more helix-shaped bearing plates affixed to a central shaft. This pile is installed by rotating the shaft into the ground to support structural loads. The advantages of helical piles are no need for boring or grout process, and ability to install with relatively light devices. The bearing capacity of the helical pile is exerted by integrating the bearing capacity of each helix plate attached to the steel shaft. In this paper, to estimate the bearing capacity of moderate-size helical piles, 6 types of helical piles were constructed with different shaft diameter, plate configuration and the penetration depth. A series of field loading tests was performed to evaluate the effect of helical pile configuration on the bearing capacity of helical pile, constructed in two different shaft diameters (i.e. 73 mm and 114 mm). In the same way, the diameter of bearing plate was also changed from 400mm to 250mm with one or three plates. As well, the penetration depth was varied from 3m to 6m to analyze the relation between the penetration depth and the bearing capacity. As a result, not only the increase of the shaft diameter, but also the number or diameter of helix bearing plates enhances the bearing capacity. Especially the configuration of the helix plate is more critical than the shaft diameter.