• Title/Summary/Keyword: light activation

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Autophagy-related protein LC3 and Beclin-1 in the first trimester of pregnancy

  • Chifenti, Barbara;Locci, Maria Teresa;Lazzeri, Gloria;Guagnozzi, Mariangela;Dinucci, Dino;Chiellini, Federica;Filice, Maria Elena;Salerno, Maria Giovanna;Battini, Lorella
    • Clinical and Experimental Reproductive Medicine
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    • v.40 no.1
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    • pp.33-37
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    • 2013
  • Autophagy is a degradation process that acts in response to environmental stressors. Recently, autophagy has been detected in normal term, preeclamptic and intrauterine growth-restricted placentas. The object of this work was to investigate the presence of autophagy in first trimester voluntary interruption of pregnancy placental villi by the expression of autophagy-related proteins, light chain 3 (LC3), and Beclin-1. In first trimester placental villi laser scanning confocal microscopy (LSCM) analysis revealed LC3 and Beclin-1 immunoreactivity prevalently located in villous cytotrophoblasts. Using LSCM, LC3, and Beclin-1 were localized to the cytoplasm of the trophoblast layer in human full-term placentas. Beclin-1 expression and LC3 activation were confirmed by western blotting. These data emphasize that autophagy activation is different among cytotrophoblasts and syncytiotrophoblasts depending on the gestational age and thus we speculate that autophagy might play a prosurvival role throughout human pregnancy.

축산 폐수의 효율적 처리를 위한 광합성 미세조류인 Spirulina platensis 배양 공정의 최적화

  • Ahn, Ju-Hee;Kim, Seong-Su;Kim, Tae-Ho;Lee, Jun-Yeup;Ohh, Sang-Jip;Lee, Jin-Ha;Lee, Hyeon-Yong
    • Microbiology and Biotechnology Letters
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    • v.24 no.4
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    • pp.519-524
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    • 1996
  • The kinetics of growing microalga, Spirulina platensis was investigated to treat swine wastes with optimum growth conditions. Temperature was varied from 15 to 40$\circ$C at three different light intensities, 6 W/m$^{2}$, 12 W/m$^{2}$ and 24 W/m$^{2}$. The specific growth rate was increased as temperature increased up to 30$\circ$C. The activation energy was estimated as 13.5 kcal/mol by an Arrhenius relationship. 0.24 (1/day) of specific growth rate was obtained from batch cultivation with 30% swine wastes, compared to 0.31 (1/day) from clean culture. It was found that Spirulina platensis was able to reduce 70-93% of PO$_{4}$$^{3-}$ -P, 67-93% of inorganic nitrogen, 80-90% of COD and 37-56% organic nitrogen by adding various concentrations of swine wastes for 12 days of batch cultivation. Rate constants for removing nitrates and phosphates in treating swine wastes were estimated as 0.17 (1/day) and 0.14 (1/day) in the first order reaction, respectively. 1.52 (g/L) of maximum cell density was maintained at 0.20 (1/day) of dilution rate in continuous culture, adding 20% swine wastes for 30 days. The chemical composition of the biomass obtained from the process showed 58.7% of protein, 11.0% of lipid and 15.6% of ash.

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Effects of Abdominal Drawing-in Maneuver With Light Load at Sitting on Transverse Abdominis Contraction in Participants With and Without Low Back Pain (앉은 자세에서 가벼운 부하를 들고 수행하는 복부드로잉 운동이 요통대상자와 건강인의 배가로근 수축에 미치는 영향)

  • Jong-Im Won
    • PNF and Movement
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    • v.21 no.2
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    • pp.243-253
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    • 2023
  • Purpose: This study aimed to investigate the effects of an abdominal drawing-in maneuver (ADIM) with a light load while sitting on transverse abdominis contraction in subjects with and without low back pain. Methods: In this study, 20 participants with chronic low back pain and 20 controls participated. Ultrasonography was used to assess the thickness of the external oblique (EO), internal oblique (IO), and transversus abdominis (TrA) muscles. Muscle thickness was measured at rest and during ADIM in three different sitting postures: (1) just sitting, (2) sitting loaded position (holding a 1 or 2 kg dumbbell in each hand), and (3) sitting loaded shoulder flexion position (holding a 1 or 2 kg dumbbell in each hand). Results: The contraction ratio (CR) and preferential activation ratio (PAR) of the TrA during ADIM had no significant interactional effect between the group and the sitting postures. However, the CR and PRA of the TrA during the ADIM showed significant differences among the three different sitting postures. The CR of the TrA during the ADIM in the sitting loaded shoulder flexion position was significantly increased compared to that in the sitting position (p<0.05). Moreover, the PRA of the TrA muscle during ADIM in sitting loaded and sitting loaded shoulder flexion positions was significantly higher than that in the sitting position (p<0.05). Conclusion: The findings suggest that ADIM in the sitting-loaded shoulder flexion position should be implemented to facilitate TrA activity.

Development of Light-weight Fire Protection Materials Using Fly Ash and Light-weight Aggregate (플라이애시 및 경량골재를 활용한 경량 내화성 마감재료 개발)

  • Song, Hun;Chu, Yong-Sik;Lee, Jong-Kyu;Lee, Sea-Hyun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.4
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    • pp.95-102
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    • 2012
  • The serious issue of tall building is to ensure the fire resistance of high strength concrete. Therefore, Solving methods are required to control the explosive spalling. The fire resistant finishing method is installed by applying a fire resistant material as a light-weight material to structural steel and concrete surface. This method can reduce the temperature increase of the reinforcement embedded in structural steel and concrete at high temperature due to the installation thickness control. This study is interested in identifying the effectiveness of light-weight fire protection material compounds including the inorganic admixture such as fly ash, meta-kaolin and light-weight aggregate as the fire resistant finishing materials through the analysis of fire resistance and components properties at high temperature. Also, this paper is concerned with change in microstructure and dehydration of the light-weight fire protection materials at high temperatures. The testing methods of fire protection materials in high temperature properties are make use of SEM and XRD. The study results show that the light-weight fire resistant finishing material composed of fly ash, meta-kaolin and light-weight aggregate has the thermal stability of the slight decrease of compressive strength at high temperature. These thermal stability is caused by the ceramic binding capacity induced by alkali activation reaction by the reason of the thermal analysis result not showing the decomposition of calcium hydrate. Developed light-weight fire protection materials showed good stability in high Temperatures. Thus, the results indicate that it is possible to fireproof panels, fire protection of materials.

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The improvement of the stability of hydrogenated amorphous silicon (수소화된 비정질 실리콘박막의 안정성향상에 관한 연구)

  • 이재희
    • Journal of the Korean Vacuum Society
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    • v.8 no.1
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    • pp.51-54
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    • 1999
  • Hydrogenated amorphous silicon (a-Si:H) films are fabricated by Argon radical annealing (ArRA). The deposition rate of continuously deposited a-Si:H film is 1.9 $\AA$/s. As ArRA time are increased to 0.5 and 1 minute, the deposition rate are increased to 2.8 $\AA$/s and 3.3 $\\AA$/s. The deposition rate of a-si:H films with 2 and 3 minutes ArRA time are 3.3 $\AA$/s. As the ArRA time is increased, the optical band gap and the hydrogen contents in the a-Si:H films are increased and slightly decreased. The light-induced degradation of ArRA treated a-Si:H films are less than that of continuously deposited a-Si:H film. The dark conductivity and the conductivity activation energy ($E_a$) of continuously deposited a-Si:H film are decreased to 1/25 in room temperature and increased to 0.09eV By 1 hour light soaking, respectively. The dark conductivity and $E_a$ of ArRA treated a-Si:H film decreased to 1/3 in room temperature and increased to 0.03eV by 1 hour light soaking, respectively. We could improve the stability of a-Si:H films under the light soaking by ArRA technique and discussed the microscopic process of ArRA technique.

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Evaluation of Visible-light activation of Cu2O-TiO2 (P-N type) Semiconductor Nanomaterials prepared by Ultrasonic-assisted Synthesis (초음파 합성 적용 Cu2O-TiO2 (P-N 타입) 반도체 나노물질의 가시광 활성 평가)

  • Shin, Seung-ho;Choi, Jeong-Hak;Kim, Ji-hoon;Lee, Joon Yeob
    • Journal of Environmental Science International
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    • v.28 no.11
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    • pp.971-981
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    • 2019
  • This study evaluated the photocatalytic oxidation efficiency of volatile organic compounds by $Cu_2O-TiO_2$ under visible-light irradiation. $Cu_2O-TiO_2$ was synthesized by an ultrasonic-assisted method. The XRD result indicated successful p-n type photocatalysts. However, no diffraction peaks belonging to $TiO_2$ were observed for the $Cu_2O-TiO_2$. The Uv-vis spectra result revealed that the synthesized $Cu_2O-TiO_2$ can be activated under visible-light irradiation. The FE-TEM/EDS result showed the formation of synthesized nanocomposites in the commercial P25 $TiO_2$, the undoped $TiO_2$, and $Cu_2O-TiO_2$ and componential analysis in the undoped $TiO_2$ and $Cu_2O-TiO_2$. The photocatalytic oxidation efficiencies of benzene, toluene, ethylbenzene, and o-xylene with $Cu_2O-TiO_2$ were higher than those of P25 $TiO_2$ and undoped $TiO_2$. These results indicate that the prepared $Cu_2O-TiO_2$ photocatalyst can be applied effectively to control gaseous BTEX.

A study on the creep characteristic of AZ31 Mg alloy at below 0.5Tm (0.5Tm 이하에서의 AZ31 마그네슘 합금 크리이프 특성에 관한 연구)

  • An, Jung-Oh;Kang, Dae-Min
    • Design & Manufacturing
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    • v.2 no.6
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    • pp.43-48
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    • 2008
  • Magnesium alloys have given high attention to the industry of light-weigh as automobile and electronics with aluminium, titanium and composite alloys due to their high strength, low specific density and good damping characteristics. But the magnesium contained structures under high temperature have the problems related to creep deformation and rupture life, which is a reason of developing the new material against creep deformation to use them safely. The purpose of this study is to predict the creep deformation mechanism and rupture time of AZ31 magnesium alloy. For this, creep tests of AZ31 magnesium alloy were done under constant creep load and temperature with the equipment including automatic temperature controller with acquisition computer. The apparent activation energy Qc, the applied stress exponent n and rupture life have been determined over the temperature range below 0.5Tm and stress range of 109~187MPa, respectively, in order to investigate the creep behavior. AZ31 Magnesium alloy identify the activation energy for creep deformation and the stress dependence to creep rate at below 0.5Tm, and then investigate the mechanism for creep deformation and creep rupture life of AZ31 Magnesium alloy.

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A Characteristics of Environmental Fraternitive Photopolymerization and Thermal Degradation on Butyl Methacrylate (부틸메타크릴레이트의 환경친화적인 광중합 및 열분해특성)

  • Choi, Jae-Wook;Seul, Soo-Duck;Lee, Nae-Woo
    • Journal of the Korean Society of Safety
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    • v.17 no.2
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    • pp.45-51
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    • 2002
  • This study is the series of photopolymerization on alkyl methacrylate(AMA) to continue further research. The objective of this work is to investigate the environmental fraternitive characteristics of photopolymerization kinetics on n-Buthyl methacrylate(BMA) and comparing the decomposition behavior to other AMA. The experiment was done in aqueous solution under the influence of photo-initiator concentration$(0.05{\sim}0.25mol/l)$, light intensity$(5000{\sim}9000{\mu}J/cm^{2})$ and monomer concentration$(2.0{\sim}6.0mol/l)$. n-BMA was polymerized to high conversion ratio using hydrogen $peroxide(H_{2}O_{2})$, and the kinetics model we have obtained is as follows. $R_{p}=K_{p}[S]^{0.24}[M]^{0.33}[L]^{153}exp^{(27.19/RT)}$ The differential method of thermogravimetric analysis(Friedman method) was used to obtain value of activation energy on decomposition reaction. The average value of it was 27.5Kcal/mol.

The study on the Ways to Activation of the Youth Starting up a Business by Korea-China FTA (한·중 FTA 체결에 따른 청년창업 활성화방안에 관한 연구)

  • KIM, Dong-Ho
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.69
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    • pp.617-632
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    • 2016
  • After contracting of Korea-China FTA, we have expected the trade volume has mutually been increasing a lot. If we are using this contract, we would make an activation of Korea economy. Especially, Korea youngman has been caught a big chance to make a business in Korea & China. For example, Xian, which is one of center of silk road, has become big strategy area. As we need to focus on this area, we will be expected to indirect influences of consumer's industries like cosmetic, fishery products, food service, eco-friendly items. Recently, the youngman starting up a business has become a trend of kinds of alternative new job. Then, we have to provide practical solution for young man. One of this solution is 'starting up a business'. In this study, I empirically investigated the relation between Korea-China FTA and the youth starting up a business. So, I believe that this study can light up on the direction of effective starting up for making & success a business in China.

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VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy

  • Lee, Mi-Ae;Kim, Jeong-A;Shin, Mee-Young;Lee, Jeong K.;Park, Soon-Jung;Lee, Kyu-Ho
    • Journal of Microbiology and Biotechnology
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    • v.25 no.2
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    • pp.302-306
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    • 2015
  • VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.