• 한국동물생명공학회지
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• 제38권4호
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• pp.209-214
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• 2023

Background: Single nucleotide polymorphisms (SNPs) are widely used genetic markers with applications in human disease diagnostics, animal breeding, and evolutionary studies, but existing genotyping methods can be labor-intensive and costly. The aim of this study is to develop a simple and rapid method for identification of a single nucleotide change. Methods: A modified Polymerase Chain Reaction Amplification of Multiple Specific Alleles (PAMSA) and high resolution melt (HRM) analysis was performed to discriminate a bovine polymorphism in the NCAPG gene (rs109570900, 1326T > G). Results: The inclusion of tails in the primers enabled allele discrimination based on PCR product lengths, detected through agarose gel electrophoresis, successfully determining various genotypes, albeit with some time and labor intensity due to the use of relatively costly high-resolution agarose gels. Additionally, high-resolution melt (HRM) analysis with tailed primers effectively distinguished the GG genotype from the TT genotype in bovine muscle cell lines, offering a reliable way to distinguish SNP polymorphisms without the need for time-consuming AS-PCR. Conclusions: Our experiments demonstrated the importance of incorporating unique mismatched bases in the allele-specific primers to prevent cross-amplification by fragmented primers. This efficient and cost-effective method, as presented here, enables genotyping laboratories to analyze SNPs using standard real-time PCR.

• 디지털산업정보학회논문지
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• 제4권4호
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• pp.63-69
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• 2008

Recently, every student would take lessons without knowing no lecture in campus life. And, If you are a university student, you tired a course to register in a crowded. For solving this problem, we choose the cellular phone. Nowadays peoples have one cellular phone to one person in korea. Using cellular phone, you can use cellular phone anywhere any time. In this paper, we studied mobile synthesis system using mobile cellular phone. According to use mobile campus system, the university and students can use easy an one to one service in ubiquitous.

• 가정과삶의질연구
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• 제20권4호
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• pp.135-148
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• 2002

The main purpose of this research was to explore the factors that encourage people to use the cyber counseling in resolving difficulties in their lives. Another purpose of this research was to investigate whether cyber counseling really helps people feel better. For this study, a structured survey questionnaire was distributed both on- and off-line, and 429 respondents returned completed survey forms. The major statistical methods used for data analysis were frequency, factor analysis, 1-test and ANOVA. The results of this study revealed that variables such as gender, age, occupation, internet skills, time of internet use, personality, and the awareness of cyber counseling made a difference in people's use of cyber counseling whenever they felt they needed it. However, cyber counseling seemed to fail to increase feelings of well being in people's lives. The implications of these findings are discussed in this article.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2005년도 추계학술대회 논문집
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• pp.350-355
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• 2005

All the facility, the structure and the rolling stock have limited life so after starting use, we must decide disuse or use when it exeeds the lifetime. Currently the subway which exeeds the lifetime appeared in our country. Structural members of rolling stock structures were occurred corrosion after passage of time and It gives a many effect in the structure strength. In this study, it accomplished a structural analysis which use FEM for the No.2 subway line which operated currently, and it investigated the structural strength change which it follows with dimension change of structural members due to corrosion

• 한국항행학회논문지
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• 제14권6호
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• pp.900-908
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• 2010

최근 소프트웨어 개발에 적용하기 시작한 객체지향 방법(OOM:Object-Oriented Method)은 독립적인 소프트웨어의 재사용을 통한 개발 비용과 시간의 단축을 강조하고 있다. 그러나 개발 기술에 대한 지식 부족과 확장성 및 성능을 배제한 설계로 많은 문제점이 나타나고 있다. 따라서 본 논문에서는 소프트웨어 개발 생명주기에서 소프트웨어 생산성 향상을 위한 효율적인 객체지향 모델링 방법을 제안하고자 한다. 제안 방법은 Use Case 모델링, 분석 모델링, 그리고 설계 모델링 방법들을 포함하고 있다.

• 한국사회복지학
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• 제62권1호
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• pp.317-342
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• 2010

본 연구는 거주시설에서 생활하는 성인 지적장애인들의 일상생활을 살펴보고자 한 것이다. 이를 위해 24시간을 관찰 기록하는 생활시간조사방법을 활용하여 거주시설 지적장애인의 일상생활활동과 그 안에서 나타나는 상호작용을 분석하였다. 생활시간연구방법 중에서는 시간수지연구방법 중 '활동의 빈도'와 생활시간 중 '생활'에 초점을 맞추는 방법을 활용하여 내용 분석하였다. 거주시설 지적장애인의 일상생활 분석은 전 국민을 대상으로 한 생활시간조사 행동분류표(2004)를 기준으로 하여, 거주시설 일상생활의 특성을 살펴보았다. 상호작용 분석은 Flanders(1963)의 언어 상호작용분석모형을 기초로, 지적장애인과 생활재활교사의 상호작용 정도, 유형 등을 제시하였다. 이 연구는 국내 최초로 거주시설장애인의 일상생활을 있는 그대로 조명하여 그들의 일상에 대한 이해를 심층적으로 구축하고, 이용자와 생활재활교사의 상호작용을 분석한 점, 생활시간조사방법을 적용한 점 등에 그 의의가 있으며, 향후 관련 연구의 중요한 기초 자료가 될 것으로 본다.

• 가족자원경영과 정책
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• 제26권4호
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• pp.39-52
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• 2022

최근 한국사회에서 출산율이 급락하면서 부모의 자녀돌봄에 대한 사회적 지지의 필요성에 대한 공감대가 높아지고 있다. 본 연구는 미취학자녀를 양육하는 맞벌이부모의 여가활동별 사용시간에 초점을 맞추어 성차와 시간효과를 살펴보고자 한다. 이를 위해 2004년과 2019년의 통계청 생활시간조사 자료를 활용하여 미취학자녀를 둔 맞벌이부부가 근무일인 평일에 작성한 시간일지를 분석하여 전반적인 생활시간 구성(필수시간, 의무시간, 여가시간)의 변화, 여가활동 유형(자원봉사, 교제, 참여, 종교, 의례, 문화·관광, 스포츠·레포츠, 게임·놀이, 미디어, 휴식, 기타 여가)에 따른 사용시간의 성차와 조사시기별 차이, 여가활동별 사용시간에 영향을 미치는 요인을 분석하였다(n=2,697). 본 연구의 주요결과는 다음과 같다. 첫째, 두 조사시기 모두 미취학자녀를 둔 맞벌이부부의 경우 전체성인집단에 비해 필수시간은 짧고, 의무시간은 길며, 여가시간은 짧은 것으로 나타났다. 둘째, 맞벌이부모의 여가활동별 시간사용량을 비교한 결과, 성차가 유의한 활동 가운데 2004년에는 종교활동을 제외한 모든 여가활동에서, 2019년에는 모든 여가활동에서 아버지가 어머니보다 더 많은 시간을 사용하였다. 아버지와 어머니 모두 총 여가시간이 2004년에 비해 2019년에 감소하였는데, 여가활동별로는 교제, 의례, 미디어 시간은 아버지와 어머니 모두 감소하였고, 종교시간은 어머니 집단에서, 휴식시간은 아버지 집단에서만 감소하였으며, 참여와 게임·놀이 시간은 모두 증가하는 추세를 보였다. 셋째, 맞벌이부모의 여가활동 유형 중 전체성인집단과 뚜렷한 차이를 보인 3가지 여가활동(스포츠·레포츠, 게임·놀이, 미디어)을 선정하여 회귀분석을 실시한 결과, 사회인구학적 변수와 생활시간 변수가 서로 다른 효과를 보였다. 이러한 연구결과는 맞벌이부모의 초기양육기 삶의 질 향상을 위한 가족정책과 부모교육프로그램 개발을 위한 기초자료로 활용될 것으로 기대된다.

• 한국토양비료학회지
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• 제44권1호
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• pp.84-90
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• 2011

It is imperative to study the hydrolysis of urea in high saline-sodic condition of a newly reclaimed tidal land in order to overcome the problems associated with use of urea fertilizer. The methodology adopted in this study tried to get a convenient way of estimating rate for N transformation needed in N fate and transport studies by reviewing pH and salt contents which can affect the microbial activity which is closely related to the rate of urea hydrolysis. The hydrolysis of urea over time follows first-order kinetics and soil urease activity in reclaimed soils will be represented by Michaelis-Menten-type kinetics. However, high pH and less microorganisms may delay the hydrolysis of urea due to decrease in urease activity with increasing pH. Therefore, the rate of urea hydrolysis should adopt $V_{max}$ referring enzyme activity ($E_0$) accounting for urease concentration which is indicative for urea hydrolysis, especially in a high saline and sodic soils.

• 한국주거학회:학술대회논문집
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• 한국주거학회 2008년도 춘계학술발표대회 논문집
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• pp.393-398
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• 2008

The environment is the important resource to use as well as to conserve for the development. Since 1990s, it has accentuated the importance of the environment and tried to conserve the environment in many areas. Among many area, the building industry has played a role to destruct the environment for providing the living decent space and brought the environmental affect. Therefore, the building industry should endeavor to mitigate the environmental affect. In building life cycle, it consumes the energy and natural resources. But it is difficult to evaluate the environmental affect during the whole building life. It is impossible to calculate the environmental affect without the much time and effort. Therefore. it requires the easy and handing program to calculate the affect to environment in each building life cycle. Until now, many program for assessing the affect to the environment have been developed and limitedly used because of the assessment scope and contents. In this paper, it aimed at developing the assessment program of LCA, focused on the energy consumption and carbon-dioxide emission compared with the domestic and foreign concerned programs. The developed program is divided into 3 areas; construction, maintenance, demolition.

• Molecules and Cells
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• 제19권3호
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• pp.350-355
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• 2005

Selectins are carbohydrate-binding cell adhesion molecules that play a major role in the initiation of inflammatory responses. Heparin can bind to P-selectin, and its anti-inflammatory property is mainly due to inhibition of P-selectin. However, the strong anticoagulant activity of heparin limits its clinical use. We prepared periodate-oxidized, borohydride-reduced heparin (RO-heparin) by chemical modification and tested its anticoagulant and anti-inflammatory activities. Activated partial thromboplastin time (aPTT) assays showed that, compared with heparin, RO-heparin had greatly reduced anticoagulant activity. Intravenous administration of this compound led to reduction in the peritoneal infiltration of neutrophils in a mouse acute inflammation model. In vitro cell adhesion experiments demonstrated that the effect of RO-heparin on inflammatory responses was mainly due to inhibiting the interaction of P-selectin with its ligands. These results indicate that RO-heparin may be a safer treatment for inflammation than heparin, especially when selectin is targeted.