• 생약학회지
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• 제48권4호
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• pp.304-313
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• 2017

These days, as the average span of population's life increases, the number patients of dementia also increases. But Research on Korean medicine is stilled limited. The research evaluates the effect of the extract from Cornus officinalis S.et Z on cognitive impairment induced by scopolamine in mice. The mice were randomly divided into five groups of ten mice. The normal group was treated with only 0.9% saline. The control group was treated with scopolamine (5 mg/kg, i.p.). The positive control group was treated with tacrin. The C100, 200 group was treated with C. officinalis extracts 100, 200 mg/kg. Memory-related behaviors were evaluated using a morris water maze and a passive avoidance test. Protein levels of BDNF, p-CREB (ser133), immunohistochemistry staining, and cholinergic activities were measured in brain tissue. The effects of C. officinalis extract significantly increased acetylcholine concentration and decreased acetylcholinesterase activity. The C. officinalis extract affected memory formation. Also, to confirm expression of protein BDNF, p-CREB (ser133) in the hippocampus, the researchers observed that immunohistochemistry and western blot increased in C. officinalis extract. These results suggest that C. officinalis provides a significant neuroprotective effect against scopolamine-induced cholinergic system and cognitive impairment.

• Preventive Nutrition and Food Science
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• 제4권2호
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• pp.113-116
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• 1999

The anticancer effect of methanol extracts from common Chinese cabbage kimchi(CC kimchi ) and organically cultivated Chinese cabbage kimchi (OC kimchi) was studied on the cell growth, MTT assay and SRB assay using AGS human gastric cancer cells. Methanol extracts from CC kimchi and OC kimchi exhibited the anticancer activites in vitro and in vivo. Methanol extract from 6 day-fermented CC kimchi and OC kimchi inhibited the growth of AGS cells by 55.2 and 60.7% , respectively. At MTT assay an dSRB assay, 6 day-fermented OC kimchi showed higher inhibition rate (MTT : 42%, SRB : 61%) than 6 day-fermented CC kimchi(MTT : 33%, SRB : 52%). Methanol extracts from 6-day fermented CC kimchi and OC reduced the tumor formation and prolonged the life span of sarcoma-180 cell injected Balb.c mouse. OC kimchi treated group resulted in the smaller tumor weight of 4.58$\pm$0.32g compared th the CC kimchi group of 5.40$\pm$0.78g and the control group of 7.50$\pm$0.54g and OC kimchi treted group (25.3 days) lived longest among control (20.2days ) and CC kimchi(23.5days) treted groups.

• 약학회지
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• 제42권4호
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• pp.345-352
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• 1998

Praecoxin A, an ellagitannin, purified from Alnus hirsuta var.microphlla was evaluated on the antitumor activity. Praecoxin A had the significant cytotoxicity to s ix tumor cell lines: human chronic myelogenous leukemia K-562, human promyelocytic leukemia HL-60, mouse leukemia P388, mouse lymphocytic leukemia L-1210, sarcoma-l8O, mouse lymphoma L5178Y except L-1210. And the most sensitive cell line was K-562 ($ED_{50}=2.43{\mu}g/ml$). The $ED_{50} of praecoxin A against HL-60, P388, L-1210, sarcoma7l8O and L5178Y were 6.28, 8.66, 10.00, 7.01, $9.32{\mu}g/ml$, respectively. Praecoxin A showed the increasing effect in life span by 36.8% on the 1st day after treatment of 10mg/kg in mice bearing sarcoma-180 tumor cells (ascitic form) via NCI (National Cancer Institute, U.S.A.) protocol in vivo assay. As a result, praecoxin A is considered to show the antitumor activity.

• KSBB Journal
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• 제13권4호
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• pp.357-363
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• 1998

The antitumor effects of mice fed with cell lysate of Lactobacillus plantarum were studied. The abdominal cancer induced by Sarcoma-180 was markedly inhibited and the expected life span was extended by 60% for the Balb/c mice fed with L. plantarum cell lysate for two weeks. A similar result was obtained for the rat inoculated with Spontaneous Osteosarcoma(SOS). The primary tumor volume of SOS was reduced by 70% for the rats fed with L. plantarum cell lysate (100mg/kg/day) for one week before the inoculation of SOS, while only 42% for the rats fed with the same amount of cell lysate for one week after the inoculation of tumor cell line, SOS. As lung was the metastasis site of SOS, the weight of lung was measured to determine the degree of metastasis inhibition by the L. plantarum cell lysate feeding. The rats fed with cell lysate for one week showed a remarkable inhibition of lung metastasis by 63%(before) and 46%(after), respectively. These results indicate that the feeding of L. plantarum cell lysate to mouse or rat can induce a strong stimulation of mucosal or systemic immune system and these effects results in an efficient antitumor activity.

• 한국잠사곤충학회지
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• 제50권2호
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• pp.126-132
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• 2012

갈색거저리를 소맥피와 가축사료를 먹이로 하여 $25{\pm}5^{\circ}C$, $65{\pm}10%RH$에서 사육한 결과 발육기간은 80.0 ~ 83.7일, 번데기크기는 18.1 ~ 18.7 mm로 유사하였고 2일간의 평균 산란수는 44.3 ~ 55.3개이었다. 갈색거저리 번데기의 평균 무게에 따라 0.12 g, 0.15 g, 0.18 g의 세 중량으로 구분하여 우화 후 성충의 일생동안 평균 산란수를 조사한 결과 각각 172.3, 191.2, 215.0개로 중량에 비례하여 증가하였다. 산란경향은 우화 2주차까지 51.8%를 산란하여 가장 왕성하였으며 그 후 급격히 감소하였다. 번데기 무게에 따른 성충의 수명은 각각 $27.1{\pm}6.9$, $30.1{\pm}7.8$, $27.4{\pm}8.5$ 일로 차이 없는 것으로 조사되었다. 갈색거저리 유충사육 용기($16{\times}21.5{\times}7cm$)에 유충 사육밀도를 100, 300, 500, 700마리로 사육한 결과 용화 시작 후 3주 이내에 67.9%가 용화되었으며 단위 공간당 사육 밀도가 증가함에 따라 번데기의 중량은 용화 시작 후 3주까지 각각 $146.9{\pm}6.5$, $142.1{\pm}8.3$, $122.0{\pm}9.6$, $118.5{\pm}5.2mg$으로 감소하였다.

• 대한조선학회논문집
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• 제60권2호
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• pp.65-75
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• 2023

Ships operated at sea for a long time are subjected to various kinds of loads, which may cause various types of damage. Such damages will eventually reduce the strength of hull structures. Therefore, it is necessary to estimate and evaluate the residual strength and remaining fatigue life of aging ships in order to secure structural safety, establish a reasonable maintenance plan, and make a judgment of life extension. For this purpose, the corrosion damage and local denting damage should be measured, fatigue damage estimation should be performed, and material properties of aged steel should be identified. For this study, in order to investigate the mechanical properties of aged steel, steel plates were obtained from a naval ship that reached the end of her life span. The specimens were manufactured from the obtained steel plates, and static and dynamic tensile tests, fatigue tests, and metallographic tests were performed. The mechanical properties obtained from the aged steel plates were compared with those of new steel plates to quantify the aging effect on the mechanical properties of marine steel materials.

• Steel and Composite Structures
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• 제26권2호
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• pp.227-239
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• 2018

Integral abutment bridges (IABs) have no joint across the length of bridge and are therefore also known as jointless bridges. IABs have many advantages, such as structural integrity, efficiency, and stability. More importantly, IABs have proven to be have both low maintenance and construction costs. However, due to the restraints at both ends of the girder due to the absence of a gap (joint), special design considerations are required. For example, while replacing the deck slabs to extend the service life of the IAB, the buckling strength of the steel girder without a deck slab could be much smaller than the case with deck slab in place. With no deck slab, the addition of thermal expansion in the steel girders generates passive earth pressure from the abutment and if the applied axial force is greater than the buckling strength of the steel girders, buckling failure can occur. In this study, numerical simulations were performed to estimate the buckling strength of typical steel girders in IABs. The effects of girder length, the width of flange and thickness of flange, imperfection due to fabrication and construction errors on the buckling strengths of multiple and single girders in IABs are studied. The effect of girder spacing, span length ratio (for a three span girder) and self-weight effects on the buckling strength are also studied. For estimation of the reaction force of the abutment generated by the passive earth pressure of the soil, BA 42/96 (2003), PennDOT DM4 (2015) and the LTI proposed equations (2009) were used and the results obtained are compared with the buckling strength of the steel girders. Using the selected design equations and the results obtained from the numerical analysis, equations for preventing the buckling failure of steel girders during deck replacement for maintenance are presented.

• Journal of Animal Science and Technology
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• 제64권1호
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• pp.166-182
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• 2022

Deer antler velvet is widely used in traditional medicine for its anti-aging, antioxidant, and immunity-enhancing effects. However, few studies have reported on the discovery of probiotic strains for deer antler fermentation to increase functional ingredient absorption. This study evaluated the ability of probiotic lactic acid bacteria to enhance the concentrations of bioactive molecules (e.g., sialic acid and gamma-aminobutyric acid [GABA]) in extracts of deer antler velvet. Seventeen strains of Lactobacillus spp. that were isolated from kimchi and infant feces, including L. sakei, L. rhamnosus, L. brevis, and L. plantarum, and those that improved the life span of Caenorhabditis elegans were selected for evaluation. Of the 17 strains, 2 (L. rhamnosus LFR20-004 and L. sakei LFR20-007) were selected based on data showing that these strains increased both the sialic acid and GABA contents of deer antler extract after fermentation for 2 d and significantly improved the life span of C. elegans. Co-fermentation with both strains further increased the concentrations of sialic acid, GABA, and metabolites such as short-chain fatty acids and amino acids. We evaluated the biological effects of the fermented antler velvet (FAV) on the antibacterial immune response in C. elegans by assessing worm survival after pathogen infection. The survival of the C. elegans conditioned with FAV for 24h was significantly higher compared with that of the control worm group fed only normal feed (non-pathogenic E. coli OP50) exposed to E. coli O157:H7, Salmonella typhi, and Listeria monocytogenes. To evaluate the protective effects of FAV on immune response, cyclophosphamide (Cy), an immune-suppressing agent was treated to in vitro and in vivo. We found that FAV significantly restored viability of mice splenocytes and immune promoting-related cytokines (interleukin [IL]-6, IL-10, inducible nitric oxide synthase [iNOS], interferon [IFN]-γ, and tumor necrosis factor [TNF]-α) were activated compared to non-fermented deer antlers. This finding indicated the protective effect of FAV against Cy-induced cell death and immunosuppressed mice. Taken together, our study suggests that immune-promoting antler velvet can be produced through fermentation using L. rhamnosus LFR20-004 and L. sakei LFR20-007.

• 대한한방종양학회지
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• 제2권1호
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• pp.75-90
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• 1996

This experiment was designed to study the antitumor effects and Activity of Natural Killer Cell of semen Tiglii plus Rhizoma Rhei. The cytotoxic and antitumor effects were evaluated on human cell lines(A549, Caki-1, LL2, Sarcoma 180, NIH/3T3) after exposure to prebrewed Semen Tiglii plus Rhizoma Rhei water extract 0.1, 0.2, 0.4, 0.8, 1.6mg/ml using in MTT assay, LDH, colony forming efficency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. 1. From the result of MTT assay, the cytotoxicity of ST(生巴豆霜), ST+RR(生巴豆霜加大黃) were concentration-dependently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR(生巴豆霜加大黃) was similar to that of ST(生巴豆霜). 2. From the result of LDH, the cytotoxicity of ST, ST +RR were concentrati -on-dependently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR was similar to that of ST. 3. The antitumor effect on A549 tumor cell from the result of colony forming efficiency showed the inhibitory effect on the growth in both group of the ST and ST+RR, the inhibitory effect on growth was low slightly in the ST+RR. 4. From the result of SRB assay, the antitumor effect on caki-1 tumor cell of ST, ST+RR showed the inhibitory effect on the growth in both group of the ST and ST+RR, the antitumor effect of ST+RR was similar to that of ST. 5. Median survival time and increased life span were increased slightly in both group of the ST and ST+RR. 6. The inhibitory effect on the growth of Sarcoma 180 and Lewis lung carcinoma tumor cell were increased slightly in both group of the ST and ST+RR. 7. The activity of NK cell was increased in the ST+RR.

• Journal of the Korean Wood Science and Technology
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• 제42권1호
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• pp.1-12
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• 2014

본 연구에서는 리기다소나무 구조용 집성재를 사용한 국내 최초 차량용 목조교량에 대한 지구온난화 영향을 평가하기 위해 전과정평가를 수행하였다. 교량연장 30 m, 교량폭원 8.4 m, 교량등급 1등급인 아치 트러스 형태의 대상 교량은 원료채취부터 제조, 수송, 시공, 사용, 해체, 건설폐기물 수송, 폐기 및 재활용까지 설계수명 50년간 총 192.56 ton $CO_2$ eq.의 온실가스를 배출한다. 전과정단계 중 원료채취 및 제조단계에서 총 온실가스 배출량의 81.14%를 배출하며 특히, 콘크리트 사용으로 인하여 82.84 ton $CO_2$ eq.의 온실가스가 배출된다. 그러나 대상 교량은 $116.57m^3$의 국산 리기다소나무 집성재를 사용하였으며, 교량을 구성하는 목재에서 104.72 ton의 이산화탄소를 저장하고 있어 이를 적용할 경우, 총 온실가스 배출량의 54.38%를 저감 가능한 것으로 도출되었다. 대상 교량과 동일한 수명과 구조를 갖는 타 교량의 철골자재를 구조용 집성재로 대체할 경우, 원료채취 및 제조단계의 온실가스 배출량을 최소 10.26%에서 최대 23.91%까지 저감 가능한 것으로 도출되었다. 본 연구의 결과는 향후 국산 목재 및 목조교량의 친환경적 우수성을 정량적으로 입증할 수 있는 기반자료로 활용 가능할 것이며, 목조교량의 친환경적 설계와 보급을 위해 활용 가능할 것으로 사료된다.