• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• 제28권2호
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• pp.13-22
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• 2015

Objective : To investigate how general characteristics and life habits of patients affect severity of acne which was determined by Korean Acne Grading system(KAGS). To adress correlation between the severity of acne and subjective stresses from which patients suffer. Methods : 86 patients answered to questionaire sheet on their general characteristics and life habits and rated their level of stresses as a number in the range between 0 and 10. Then an oriental medical doctor evaluated subject's acne grades, based of KAGS, through observations on papules, nodules, and scars before (medical) treatments. All data (collected) were analyzed using SPSS 22 (software for windows). To clearly demonstrate association of subjects general characteristics, life habits with KAGS grades, Chi-square tests were performed. The correlation between the KAGS grades and the level of stresses was determined using one-way ANOVA followed by Scheffe's post-hoc analysis. Results & Conclusion : Majority of subjects were women, students between 15 and 25 years old, and patients with normal range of Body Mass Index(BMI). Dysmenorrhea presence and premenstrual exacerbation of acne had statistically significant relationship, suggesting their potential influences on KAGS grades as major factors.

• Molecules and Cells
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• 제40권10호
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• pp.697-705
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• 2017

The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.175-181
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• 2012

Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184 (Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184 fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.

• Journal of The Korean Society of Grassland and Forage Science
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• 제43권1호
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• pp.42-49
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• 2023

The plant-specific NAC transcription factors control various biological processes, including plant development and stress responses. We have isolated an ANAC032 gene, one of the NAC transcription factor family, which was highly activated by multi-abiotic stresses, including high salt and drought in Arabidopsis. Here, we generated transgenic plants constitutively expressing ANAC032 and its knockout to identify the functional roles of ANAC032 in Arabidopsis under abiotic stress responses. The ANAC032-overexpressing plants showed enhanced tolerance to salinity and drought stresses. The anac032 knockout mutants were observed no significant changes under the high salt and drought conditions. We also monitored the expression of high salt and drought stress-responsive genes in the ANAC032 transgenic plants and anac032 mutant. The ANAC032 overexpression upregulated the expression of stress-responsive genes, RD29A and ERD10, under the stresses. Thus, our data identify that transcription factor ANAC032 plays as an enhancer for salinity and drought tolerance through the upregulation of stress-responsive genes and provides useful genetic traits for generating multi-abiotic stress-tolerant forage crops.

• Korean Journal of Human Ecology
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• 제17권2호
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• pp.255-269
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• 2008

The study is to investigate how much middle-aged male worker's life stress affect their psychological well-being. The subject group was 223 male workers aged from 40 to 59 with a wife and children. The results are as follows: 1) the middle-aged male workers perceive an economic life stress as a most stressful problem, and mostly use a 'direct-positive' coping behavior(CB) type to settle the stress among 4 stress CB types. 2) Economic life and marital stress, 'indirect-active' and 'direct-active' CB give deep effects on their life satisfaction. 3) The effecting factors on their depression are work stress, marital stress, health stress, a 'direct-passive', an 'indirect-active', an 'indirect-passive' CB and their income. In order to increase their life satisfaction and to decrease their depression, concludingly, it is most significant for them to have a strong marital relationship and actively cope with life stress.

• The Journal of Economics, Marketing and Management
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• 제8권1호
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• pp.29-35
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• 2020

Purpose - In this study, among the various stresses experienced by single-family senior citizens, we will focus on economic and financial stress and help to comprehend it generally, examine the differences in economic and financial stress and coping behaviors caused by the background variables in the aged group, and verify the variables that affect economic and financial stress, response behavior, and life satisfaction. Research design, data, and methodology - First of all, frequency analysis and technical statistics were conducted to identify the general characteristics of the elderly (characteristics of demographic sociology, social relationships) and the satisfaction level of life. Secondly, Hierarchical Multiple Regression Analysis was conducted to analyse influential factors on life satisfaction. Results - looking at the demographic characteristics of the elderly showed that in case the spouse exists, the level of satisfaction in life was shown to be higher. Next, looking at the characteristics of economic stress showed that the lower the economic stress was, the higher the satisfaction level of life was shown. Conclusions - Since stress has been identified as the most influential variable in life satisfaction through this study, we believe that economic and financial stress should be studied together as a parameter to find out which factors influence life satisfaction, or studies to reduce stresses for experts and senior party members altogether is also needed.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제18권12호
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• pp.443-451
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• 2017

The purpose of this research is to examine ways to improve the quality of life of elderly people living alone in urban areas by identifying their stress, social support, and quality of life as well as analyzing the relationships between these factors. In this research, a questionnaire survey was conducted for approximately two weeks including 232 elderly people aged 65 years and above who were using 10 senior welfare centers in the Seoul and Gyeonggi regions. The results of the analysis based on the collected data showed that of the stresses experienced by elderly people living alone in urban areas, the stresses of health, family, and psychological and social stresses had negative effects on social support and quality of life. Also, it was found that the social support of elderly people living alone had positive effects on the quality of life. Additionally, it was found that social support had a mediating effect in the relationship between stress and quality of life of elderly people living alone in urban areas. In other words, social support plays a buffer role in enhancing the quality of life regarding health, family, economy, and psychological and social stresses. This research has significant implications in that it was conducted on elderly people living alone, since elderly people living alone are not only under more stressful circumstances in a vulnerable environment than other seniors but also their quality of life is much lower. The implications of this research are that, from a social point of view, these elderly people living alone should no longer be neglected and that differentiated policy interventions for elderly people living alone is required.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2011년도 임시총회 및 추계학술발표회
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• pp.14-14
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• 2011

Polyamines (putrescine, spermidine and spermine) play pivotal roles in plant defense to different abiotic and biotic stresses. In order to understand the function of ginseng spermidine synthase gene, a key gene involved in biosynthesis of polyamines, transgenic plant was generated in Arabidopsis. The transgenic plants exhibited high levels of polyamines compared to the untransformed control plants. We investigated the tolerance capacity of transgenic plants to abiotic stresses such as salinity and copper stress. In addition, transgenic plants also showed increased resistance against one of the important fungal pathogens of ginseng, the wilt causing Fusarium oxysporum and one of important bacteria, bacterial blight causing Pseudomonas syringae. However, an activity of the polyamine catabolic enzyme, diamine oxidase (DAO) was increased significantly in F. oxysporum and P. syringae infected transgenic plant. Polyamine catabolic enzymes which may trigger the hypersensitive response (HR) by producing hydrogen peroxide ($H_2O_2$) seem act as an inducer of PR proteins, peroxidase and phenyl ammonium lyase activity. The transgenic plants also contained higher antioxidant enzyme activities, less MDA and $H_2O_2$ under salt and copper stress than the wild type, implying it suffered from less injury. These results strongly suggest an important role of spermidine as a signaling regulator in stress signaling pathways, leading to build-up of stress tolerance mechanisms.

• Plant Biotechnology Reports
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• 제5권1호
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• pp.71-77
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• 2011

Drought and salinity are the most important abiotic stresses that affect the normal growth and development of plants. Glycine betaine is one of the most important osmolytes present in higher plants that enable them to cope with environmental stresses through osmotic adjustment. In this study, a betaine aldehyde dehydrogenase (BADH) gene from spinach under the control of the stress-induced promoter rd29A from Arabidopsis thaliana was introduced into potato cultivar Gannongshu 2 by the Agrobacterium tumefaciens system. Putative transgenic plants were confirmed by Southern blot analysis. Northern hybridization analysis demonstrated that expression of BADH gene was induced by drought and NaCl stress in the transgenic potato plants. The BADH activity in the transgenic potato plants was between 10.8 and 11.7 U. There was a negative relationship (y = -2.2083x + 43.329, r = 0.9495) between BADH activity and the relative electrical conductivity of the transgenic potato plant leaves. Plant height increased by 0.4-0.9 cm and fresh weight per plant increased by 17-29% for the transgenic potato plants under NaCl and polyethylene glycol stresses compared with the control potato plants. These results indicated that the ability of transgenic plants to tolerate drought and salt was increased when their BADH activity was increased.

• Journal of Life Science
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• 제23권4호
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• pp.479-486
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• 2013

The DNA-binding protein from starved cells (DPS), originally identified as a DNA binding protein in Escherichia coli, is known to play an important role in DNA protection. The aim of this study was to evaluate the functional roles of DPS in E. coli against various kinds of external stresses by comparing the properties of wild-type E. coli cells and dps knockout mutant E. coli (${\Delta}dps$) cells. Under various stress conditions, we measured the cell growth of the wild-type E. coli and the dps knockout mutant E. coli (${\Delta}dps$) cells using a UV spectrophotometer. The growth rate of the cells was compared to investigate the functional roles of the DPS protein in E. coli. In comparison to the properties of the wild-type E. coli cells, the dps knockout mutant E. coli (${\Delta}dps$) cells showed highly sensitive phenotypes under various stress conditions, such as heat shock, acidic pH, nutrient deficiency, and different concentrations of reactive oxygen species (ROS), suggesting that DPS plays key roles in E. coli in combating diverse external stresses. The DPS DNA-binding protein in E. coli plays crucial roles in bacterial cell growth and in the protection of the cells from environmental stresses by tightly binding and preserving their DNA molecules.