• 한국산학기술학회논문지
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• 제11권9호
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• pp.3148-3154
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• 2010

본 논문에서는 충격손상 복합재료의 피로수명 저하 특성과 이의 변동성을 통계적으로 분석하였다. 충격손상 복합재료의 잔류강도는 2모수 Weibull 분포에 잘 적합되었으며 충격에너지의 증가에 따라 잔류강도의 변동성은 감소 하였다. 복합재료의 피로수명은 충격손상에 의하여 크게 저하되었으며 또한 이의 피로수명에 대한 충격손상의 영향은 작용 응력진폭에 따라 변화함을 알 수 있었다. 또한 2모수 Weibull 분포를 이용하여 충격손상 복합재료의 피로수명의 변동성을 추정하였으며 충격에너지의 증강 및 응력진폭의 감소에 따라 피로수명의 변동성은 점차 감소하였다.

• 대한기계학회논문집
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• 제15권5호
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• pp.1455-1461
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• 1991

본 연구에서는 Kachanov의 재료손상(material damage)모델을 이용하여 새로운 수명예측식을 만들고, 이 수명예측식의 타당성을 조사하기 위하여, 최근에 발표된 크 립 수명과 기공분포와의 실험결과와 비교하였다.

• International Journal of Naval Architecture and Ocean Engineering
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• 제8권5호
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• pp.487-495
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• 2016

In the offshore project such as semi-submersible FPU and FPSO, the free fall type life boat called TEMPSC (Totally Enclosed Motor Propelled Survival Craft) has been installed for the use of an emergency evacuation of POB (People on Board) from the topside platform. For the design of life boat arrangement for semi-submersible FPU in the initial design stage, the drop height and launch angle are required fulfill with the limitation of classification society rule and Company requirement, including type of approval as applicable when intact and damage condition of the platform. In this paper, we have been performed the numerical studies to find proper arrangement for the life boats consider drop height in various environmental conditions such as wave, wind and current. In the calculations, the contributions from static and low frequency (LF) motions are considered from the hydrodynamic and mooring analysis as well as damage angle from the intact and damage stability analysis. Also, Air-gap calculation at the life boat positions has been carried out to check the effect on the life boat arrangement. The air-gap assessment is based on the extreme air-gap method includes the effect of 1st order wave frequency (WF) motions, 2nd order low frequency roll/pitch motion, static trim/heel and set down.

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.984-997
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• 2021

This study sought to evaluate DNA damage and repair in porcine postovulatory aged oocytes. The DNA damage response, which was assessed by H2A.X expression, increased in porcine aged oocytes over time. However, the aged oocytes exhibited a significant decrease in the expression of RAD51, which reflects the DNA damage repair capacity. Further experiments suggested that the DNA repair ability was suppressed by the downregulation of genes involved in the homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways. The expression levels of the cell cycle checkpoint genes, CHEK1 and CHEK2, were upregulated in porcine aged oocytes in response to induced DNA damage. Immunofluorescence results revealed that the expression level of H3K79me2 was significantly lower in porcine aged oocytes than in control oocytes. In addition, embryo quality was significantly reduced in aged oocytes, as assessed by measuring the cell proliferation capacity. Our results provide evidence that DNA damage is increased and the DNA repair ability is suppressed in porcine aged oocytes. These findings increase our understanding of the events that occur during postovulatory oocyte aging.

• 한국생산제조학회지
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• 제22권1호
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• pp.36-44
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• 2013

This study investigates life, damage and durability through the analyses of fatigue load and vibration on disk brake models of A, B and C. Maximum equivalent stress is happened at the inside of disk brake on these models. As there are A, B and C models by order of life, model A has the most stable strength on fatigue analysis, The deformations at 3 kinds of models become nearly same on natural frequency analysis. The maximum total deformation and equivalent stress is shown at 1617Hz by harmonic vibration analysis on these models. As there are A, B and C models by order of deformation and stress, model A becomes lowest and safest. This study result can be effectively utilized with the design of brake disk in order to improve durability and prevention against its fatigue damage and vibration.

• BMB Reports
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• 제53권2호
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• pp.112-117
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• 2020

A recent study suggested that methylation of ubiquitin-like with PHD and RING finger domain 1 (UHRF1) is regulated by SET7 and lysine-specific histone demethylase 1A (LSD1) and is essential for homologous recombination (HR). The study demonstrated that SET7-mediated methylation of UHRF1 promotes polyubiquitination of proliferating cell nuclear antigen (PCNA), inducing HR. However, studies on mediators that interact with and recruit UHRF1 to damaged lesions are needed to elucidate the mechanism of UHRF1 methylation-induced HR. Here, we identified that poly [ADP-ribose] polymerase 1 (PARP1) interacts with damage-induced methylated UHRF1 specifically and mediates UHRF1 to induce HR progression. Furthermore, cooperation of UHRF1-PARP1 is essential for cell viability, suggesting the importance of the interaction of UHRF1-PARP1 for damage tolerance in response to damage. Our data revealed that PARP1 mediates the HR mechanism, which is regulated by UHRF1 methylation. The data also indicated the significant role of PARP1 as a mediator of UHRF1 methylation-correlated HR pathway.

• Structural Engineering and Mechanics
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• 제38권4호
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• pp.443-457
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• 2011

The loss of strength in a structure as a result of cyclic loads over a period of life time is an important phenomenon for the life-cycle analysis. Service loads are accentuated at the areas of stress concentration, mainly at the connection of components. Structural components unavoidably are affected by defects such as surface scratches, surface roughness and weld defects of random sizes, which usually occur during the manufacturing and handling process. These defects are shown to have an important effect on the fatigue life of the structural components by promoting crack initiation sites. The value of equivalent initial flaw size (EIFS) is calculated by using the back extrapolation technique and the Paris law of fatigue crack growth from results of fatigue tests. We try to analyze the effect of EIFS distribution in a multiple site damage (MSD) specimen by using the extended finite element method (XFEM). For the analysis, fatigue tests were conducted on the centrally-cracked specimens and MSD specimens.

• Structural Engineering and Mechanics
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• 제28권4호
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• pp.387-410
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• 2008

The fatigue damage accumulation rates of horizontally curved thin walled box-girder bridge have been estimated from vehicle-induced dynamic stress history using rain flow cycle counting method in the time domain approach. The curved box-girder bridge has been numerically modeled using computationally efficient thin walled box-beam finite elements, which take into account the important structural actions like torsional warping, distortion and distortional warping in addition to the conventional displacement and rotational degrees of freedom. Vehicle model includes heave-pitch-roll degrees of freedom with longitudinal and transverse input to the wheels. The bridge deck unevenness, which is taken as inputs to the vehicle wheels, has been assumed to be a realization of homogeneous random process specified by a power spectral density (PSD) function. The linear damage accumulation theory has been applied to calculate fatigue life. The fatigue life estimated by cycle counting method in time domain has been compared with those found by estimating the PSD of response in frequency domain. The frequency domain method uses an analytical expression involving spectral moment characteristics of stress process. The effects of some of the important parameters on fatigue life of the curved box bridge have been studied.

• Wind and Structures
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• 제31권3호
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• pp.217-227
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• 2020

Based on translation models, both Gaussian and non-Gaussian wind fields are generated using spectral representation method for investigating the influence of non-Gaussian characteristics and directivity effect of wind load on fatigue damage of wind turbine. Using the blade aerodynamic model and multi-body dynamics, dynamic responses are calculated. Using linear damage accumulation theory and linear crack propagation theory, crack initiation life and crack propagation life are discussed with consideration of the joint probability density distribution of the wind direction and mean wind speed in detail. The result shows that non-Gaussian characteristics of wind load have less influence on fatigue life of wind turbine in the area with smaller annual mean wind speeds. Whereas, the influence becomes significant with the increase of the annual mean wind speed. When the annual mean wind speeds are 7 m/s and 9 m/s at hub height of 90 m, the crack initiation lives under softening non-Gaussian wind decrease by 10% compared with Gaussian wind fields or at higher hub height. The study indicates that the consideration of the influence of softening non-Gaussian characteristics of wind inflows can significantly decrease the fatigue life, and, if neglected, it can result in non-conservative fatigue life estimates for the areas with higher annual mean wind speeds.

• Food Science and Biotechnology
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• 제16권6호
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• pp.1064-1068
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• 2007

We evaluated the effect of soybean dongchunghacho [SD, cultivated dongchunghacho fungus (Paecilomyces tenuipes) on soybeans] on dimethylhydrazine (DMH)-induced DNA damage and oxidative stress in male F344 rats. The animals were divided into 3 groups and fed a casein-based high-fat, low fiber diet without (DMH group) or with 13%(w/w) of soybean (DMH+S group), or SD (DMH+SD group). One week after beginning the diets, rats were treated weekly with DMH (30 mg/kg, s.c.) for 6 weeks; dietary treatments were continued for the entire experiment and endpoints measured at 9 weeks after the first DMH injection. SD supplementation reduced DMH-induced DNA damage in colon cells and reduced plasma lipid peroxidation. Thus, SD may have therapeutic potential for early-stage colon carcinogenesis.