• 제목/요약/키워드: life culture

검색결과 5,651건 처리시간 0.032초

Production of Beijerinckia indica HS-2001 in Fed-batch and continuous culture.

  • 양재균;서형필;신명교;이진우
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XII)
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    • pp.245-248
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    • 2003
  • As a result of broth substitutions when each culture-mediums were difference, whole culture-medium was found to be best feeding solution for production of PS-7 by B. indica. Maximal production of PS-7 was 1$10.0\;g/{\ell}$ and its conversion rate from 2% (w/v) glucose to PS-7 was 50%. After 48 hr, 50%(v/v) medium of working volume began to substitute in 7L jar fermenter. Production of PS-7 increased after 48hr, recovered productivity of PS-7. Following this preliminary culture, the resultant culture was subjected to continuous flow conditions controlled that the dilution rate were $0.01\;{\sim}\;0.04\;h^{-1}$. Production of PS-7 increased at dilution rate $0.0100\;h^{-1}$ whereas productivity of PS-7 decreased gradually in dilution rate $0.0200\;{\sim}\;0.0400\;h^{-1}$. Maximal production of PS-7 was $10.0\;g/{\ell}$ in continuous culture.

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항공사 승무원의 조직문화, 개인특성, 일중독, 직장-가정 갈등이 삶만족에 미치는 영향 - 항공 승무원을 중심으로 - (The Influence of Organizational Culture, Personal Characteristics, Workaholics, Work-Family Conflict on Life Satisfaction : Focused on the Flight Attendants on the Airline)

  • 이정아
    • 품질경영학회지
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    • 제47권4호
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    • pp.861-873
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    • 2019
  • Purpose: The purpose of this study is to investigate the effects of organizational culture, personality Traits, work addiction and workplace family conflict on the life satisfaction of Korean Air crew. Methods: The collected data through the survey were analyzed using structure equation model analysis The sample used for the empirical analysis is a total of 578 questionnaires. Results: Empirical analysis summarized next. Organizational culture and personality characteristics have some effects on work poisoning. However, the compulsion of work driven does not affect work-family conflict, Family-Work Conflict does not affect Life Satisfaction. Conclusion: This paper has a contribution in that it extends the range of the academic research concerning Workaholic. Also this paper provides implications on effective human resource management method suitable for airline industry.

Effect of Nutrient Nitrogen on the Degradation of Pentachlorophenol by White Rot Fungus, Phanerochaete chrysosporium

  • Chung, Nam-Hyun;Kang, Gu-Young;Kim, Gyu-Hyeok;Lee, Il-Seok;Bang, Won-Gi
    • Journal of Microbiology and Biotechnology
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    • 제11권4호
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    • pp.704-708
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    • 2001
  • The effect of nutrient nitrogen on the degradation of pentachlorophenol (PCP) by Phanerochaete chrysosporium in a liquid culture was investigated. PCP disappeared at almost the same rate in both nutrient nitrogen-sufficient (NS) and -limited (NL) sttionary cultures. However, more pentachloroanisole (PCA) was accumulated in the NS culture than in the NL culture. The effect of nitrogen on the degradation of PCA was also tested in both cultures. PCA disappeared faster in the NL culture than in the NS culture, indicating that the lower accumulation of PCA during the degradation of PCP in the NL culture was due to the faster degradation of PCA in the NL culture than in the NS culture. In another experiment, PCA was added to shaking cultures rather than stationary cultures to search for any other metabolite(s). While no other metabolite but PCA was found in the NS stationary culture, 2,4,5,6-tetrachloro-2,5-cyclohexadiene-1,4-dione(TCHD) was found as the only indentifiable product in the NL shaking culture. Thus, PCP would appear to be metabolized to TCHD via PCa or directly oxidized to TCHD by lignin peroxidase. Since all the above results indicate that no innocuous metabolite was formed during the degradation of PCP by the fungus, it is quite feasible to use the fungus in the biotreatment of PCP.

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음식문화거리조성 성공 요인에 관한 연구 (A Study on the Successful Factors in Building Food Culture Streets)

  • 엄영호
    • 한국조리학회지
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    • 제12권1호
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    • pp.157-172
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    • 2006
  • Food Culture is formed in a unique culture heritage in some areas harmonized with social factors mixed with geographical conditions, natural environment, culture and religion. The more the life skill develops highly, the more people focus on improvement of the life skill in order to realize a delightful and convenient life, and plan an increase of production. Recently food-service companies have changed very quickly with economic environment. Such change will be continued and its speed will be more faster than now. Many food-service companies will disappear for weak competition, if they don’t manage adequately in such a case. Therefore, food-service companies have to search for an efficient, unique and creative management strategy. In such a viewpoint, Kyunggi-do designated five local cities as a model of Food Culture Streets greeting Welcoming Year of Kyunggi-do in 2005. The purpose of this study is to serve the healthy food to visitors visiting the Food Culture Streets and to do public relations about the excellence of their traditional food. In addition, this study includes improving tastes and qualities of food through education about cooking skills, management strategies and the best service with unique and creative menu development. Accordingly, we need to analyze the factors continuously on the business of food culture streets, and intend to use them as a database for high quality education and activity of food service industry in the future.

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고추의 소포자 배양 시 전처리 배지, 새 배지의 첨가, 및 배양 용기의 크기가 배의 생산에 미치는 영향 (Influence of pretreatment medium, fresh medium addition, and culture plate size on the production of embryos in isolated microspore culture of hot pepper (Capsicum annuum L.))

  • 박은준;김진애;김문자
    • Journal of Plant Biotechnology
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    • 제36권2호
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    • pp.184-192
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    • 2009
  • The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment ($32{\pm}1{^{\circ}C}$), high frequency embryo production was obtained when the sucrosestarvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from $3.5{\times}1.0$ cm to $6.0{\times}1.5$ cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.

인간생활의 관점에서 본 현대 대중 소비문화 (A Study on the Culture of Modern Mass Consumption in View of Human Decent Life)

  • 박순희
    • 가족자원경영과 정책
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    • 제12권3호
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    • pp.45-58
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    • 2008
  • The purpose of this study is to determine how the culture of modern mass consumption is understood in determining a decent lifestyle. This study examined a variety of literature on this problem. The important results are as follows. The culture of consumption has evolved through the increase of productivity in modern industry. People's desires have instigated a developing consumption culture: the concepts of advertising, fashion, over-consumption, and conspicuous consumption. The dissemination of this new consumption culture in an information-rich society, facilitated by the popularization of the Internet, has started to influence every nook and comer of our lives. This change of lifestyle has resulted not only in positive effects, but also in negative ones, such as distortion of information, and inequality. Thus the culture of modern mass consumption has created a new concept of class order and has reinforced the unbalance between opinion-leaders and opinion-followers. It has also increased the ratio of needs to wants. Consumption makes escape and sensual pleasure possible. The conclusion of this study is that the culture of modern mass consumption has not upheld its original purpose, that is, the qualitative enrichment of life through consumption. It has only caused the disintegration of individuality.

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구술을 통한 전통세대의 음식문화특성 연구 (A Study on the Food-culture's Property of the Traditional Generation through the Oral Interview)

  • 김미혜;정혜경
    • 한국식생활문화학회지
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    • 제24권6호
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    • pp.613-630
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    • 2009
  • This thesis, which involves honest life stories of members of the ìtraditionalî Korean generation that lived through the turbulent times of the first half of the twentieth century, assesses the meaning and import of Korean cuisine during an individual Korean's lifetime, as well as the relevant properties of the culinary culture of the traditional generation and how those properties continue to influence the present generation of Koreans. Thus, traditional Korean culinary culture was subdivided into the following four aspects, each of which were exemplified by representative examples. The first of these is slow-food dietary life, which is exemplified by fermented foods. The development of side dishes (panchan) based on fermentation - kimchi, different types of soy and bean paste, salted seafoods, dishes of dried radish or cucumber slices seasoned with soy sauce, and so on - made the quantitative and qualitative supplementation of food possible for traditional Koreans. The second of these aspects, referred to as friendly dietary life, is exemplified by self-sufficiently produced foods. The system of many species and small production suitable with the season made it possible to produce food from sustainable ecological systems and to maintain locally grown food-cultures, each of which was distinguished from others by a local specialty product. The third aspect of the traditional Korean culinary culture involves the same use of medicinal roots and plant materials for foodstuff, and this is exemplified by the use of foods to cure and prevent diseases. The notion, for example, that 'boiled rice is an invigorant' is characteristic of the notion that diet can function in a preventative medical context, and other similar Korean notions illustrate the importance, also, of the curative properties of food. The fourth and final aspect of traditional Korean culinary culture identified herein is creative dietary life, which can be viewed essentially as a Korean adaptation to the turbulence of life during the early $20^{th}$ century in Korea. This trend is exemplified by many Korean foods that were created in response to foreign influences, such as onions, cabbages, curry, etc. which found their place in overall Korean culture through the age of Japanese settlement, as well as the Korean war.

A Study on the Internet and Teenagers' Culture

  • Jang, Jung-Min;Cho, Hyun-Soo;Jung, Hye-Jung
    • Journal of the Korean Data and Information Science Society
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    • 제14권3호
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    • pp.511-523
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    • 2003
  • Internet has become an important part of teenagers' life and has changed the life style of teenagers. Internet can be an useful tool for educating teenagers and also can be misused as a cyberspace for social problems. Recognizing that teenagers spend most of their free time enjoying internet and they form their own new life culture, it is needed to develop practical strategies for maximizing the positive effect of internet on the teenagers' culture. The purpose of this research is to suggest a desirable way for forming teenagers' healthy internet-based culture. The research was conducted by the survey of teenagers in the city of Pyongtaek on their use of internet and their problems The survey shows that most of teenagers are using internet in order to release the stress and most of them are hooked on it. It also indicates that cyber police and volunteer groups should be formed to help the desirable teenagers' internet-based culture.

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청소년의 전통 의생활 문화 인식 고취를 위한 가정과 교수.학습 과정안 개발 및 적용 -고등학교 1학년 '가정생활 문화의 변화' 단원을 중심으로- (Development and application of Technology.Home Economics Teaching-Learning Lesson Plan on Clothing Lifes to Raise youth's awareness of traditional clothing culture)

  • 반윤자;김남은;이혜자
    • 한국가정과교육학회지
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    • 제23권4호
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    • pp.1-17
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    • 2011
  • 본 연구는 청소년의 전통 의생활 문화인식을 증진시키기 위하여 의생활 단원 내용을 중심으로 교수 학습 과정안을 개발하고 적용하였다. 7차 교육과정 고등학교 1학년 교과서 12종과 7차 개정교육과정을 분석하고 이를 바탕으로 '가정생활문화 변화'단원을 중심으로 '전통문화', '한복의 변천사', '한복의 색채', '한복의 문양과 소재', '한복의 세계화' 5개의 학습 주제를 선정하였다. 선정한 주제에 대해, 5차시의 교수 학습 과정안 및 48종의 수업자료(학생자료 22개, 수업자료 22개, 교사 자료 4개)를 개발하고 수업에 적용하였다. 청소년들의 전통의생활 문화에 대한 인식도는 사전검사 평균에 비해 사후 검사 평균이 높게 나타난 것으로 보아 본 연구의 교수 학습 과정안이 전통 의생활 전통문화에 대한 올바른 인식, 외래문화에 대한 수용, 전통 문화에 대한 긍정적 자세 등 전통 문화 인식 고취에 효과적이었다고 할 수 있다. 전통의생활 교육에 대한 인식 또한 유의미하게 향상되어 전통 문화교육의 중요성을 알 수 있었다. 이에 본 연구자는 의생활 문화 교육과 관련하여 더 다양한 전통문화 관련 주제가 개발되어야 하며 더 강화되어야 한다고 제언하는 바이다. 뿐 아니라 식생활, 주생활 분야에도 전통문화 교수 학습 과정안이 개발되고 적용되는 등 다양한 전통문화 계승 교육 연구가 이루어질 필요가 있다.

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Feeder Independent Culture of Mouse Embryonic Stem Cells

  • Kim, Myoung Ok;Ryoo, Zae Young
    • Reproductive and Developmental Biology
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    • 제36권4호
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    • pp.291-294
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    • 2012
  • Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.