• 한국버섯학회지
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• 제19권2호
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• pp.81-87
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• 2021

Roridin E, produced by Podostroma cornu-damae, is a mycotoxin with anticancer activity. To increase the content of roridin E, submerged culture conditions were optimized using response surface methodology. Three factors, namely, medium initial pH, incubation time and agitation speed were optimized using a Box-Behnken design. The optimum submerged culture conditions to increase the content of roridin E included a medium with an initial pH of 4.0, an incubation time of 12.90 days, and an agitation speed of 63.03 rpm. The roridin E content in the submerged culture, under the aforementioned conditions, was 40.26 mg/L. The findings of this study can help lower the current price of roridin E and promote its related research.

• 한국수정란이식학회지
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• 제29권3호
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• pp.207-219
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• 2014

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

• 대한간호학회지
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• 제45권6호
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• pp.879-889
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• 2015

Purpose: The purpose of this study was to construct and test a structural equation model of quality of work life for clinical nurses based on Peterson and Wilson's Culture-Work-Health model (CWHM). Methods: A structured questionnaire was completed by 523 clinical nurses to analyze the relationships between concepts of CWHM-organizational culture, social support, employee health, organizational health, and quality of work life. Among these conceptual variables of CWHM, employee health was measured by perceived health status, and organizational health was measured by presenteeism. SPSS21.0 and AMOS 21.0 programs were used to analyze the efficiency of the hypothesized model and calculate the direct and indirect effects of factors affecting quality of work life among clinical nurses. Results: The goodness-of-fit statistics of the final modified hypothetical model are as follows: ${\chi}^2=586.03$, ${\chi}^2/df=4.19$, GFI=.89, AGFI=.85, CFI=.91, TLI=.90, NFI=.89, and RMSEA=.08. The results revealed that organizational culture, social support, organizational health, and employee health accounted for 69% of clinical nurses' quality of work life. Conclusion: The major findings of this study indicate that it is essential to create a positive organizational culture and provide adequate organizational support to maintain a balance between the health of clinical nurses and the organization. Further repeated and expanded studies are needed to explore the multidimensional aspects of clinical nurses' quality of work life in Korea, including various factors, such as work environment, work stress, and burnout.

• 한국미생물·생명공학회지
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• 제51권1호
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• pp.10-17
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• 2023

Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 10⁷ colony-forming unit (CFU)/ml for fermented milk products and 10⁸ CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

• Journal of Microbiology
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• 제40권1호
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• pp.82-85
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• 2002

The aerobic batch growth of Issatchenkia orientalis DY252 with glucose and fructose medium was investigated at 32$\^{C}$ and pH 5.0. Aerobic ethanol production was evident with yeast I, orientalis. A diauxic lag of about 1 h between growth on glucose and growth on ethanol during batch culture was observed. However, no diauxic growth occurred with fructose. As the incubation temperature was increased from 32 to 39$\^{C}$, viability at the end of each batch culture declined significantly, from 93 to 43%, Unlike the effect of temperature, viability was not greatly affected by incubation pH, and cell yield values in a range of 0.45-0.48 were obtained. In order to overcome overflow metabolism, a fedbatch culture under glucose limitation was carried out. Compared with aerobic batch culture, about 10% improvement in cell yield was achieved with a fed-batch culture in optimal conditions.

• 중환자간호학회지
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• 제15권3호
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• pp.23-36
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• 2022

Purpose : This study aimed to identify the factors influencing the professional quality of life of intensive care unit (ICU) nurses working in university hospitals. Methods : A survey was conducted on 171 ICU nurses in university hospitals in B City, South Korea. This study used the Professional Quality of Life instrument, which consists of three subscales, namely, compassion satisfaction, burnout, and secondary traumatic stress. Data were analyzed using stepwise multiple regression analysis. Results : Compassion satisfaction was influenced by resilience, ICU job satisfaction, and innovation-oriented culture, and these variables explained 37.1% of the variance in compassion satisfaction. Burnout was influenced by resilience, a hierarchy-oriented culture, and ICU job satisfaction, and these variables explained 42.9% of the variance in burnout. Secondary traumatic stress was influenced by a task-oriented culture and resilience, and these variables explained 12.5% of the variance in secondary traumatic stress. Conclusion : These findings suggest the importance of improving resilience and job satisfaction to enhance the professional quality of life in ICU nurses. Moreover, creating an innovation-oriented culture rather than a hierarchical and task-oriented culture can effectively improve the professional quality of life of ICU nurses.

• 아동학회지
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• 제30권6호
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• pp.167-181
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• 2009

"The Child culture" is defined as a children's life style in which they interpret their life-situations, using their comprehension system and living out their lives based on those interpretations. Although the current Korean culture of "studying (or learning)" which is referred to as a social phenomenon restraining lively childhood of children, the ideal child culture surely exists. For this research, the total number of 1,049 articles and dissertations published around the year 2000 were analyzed from dual perspectives : value and life of both children and adults. The research literature was reviewed in respect to the following categories : 1) the dimension of how children deal with home life including food, clothing, and shelter, 2) the domain within the boundary of elementary school to understand how they spend their important time, and 3) the domain out of elementary school to understand how they spend their leisure time.

• 한국조리학회지
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• 제9권1호
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• pp.101-120
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• 2003

The today society comes wind important position we and the economic room gets and our dietary life simple ´it eats,´ is not only the act which is primary work, ´it enjoys,´, ´appreciates´with culture dimension to execute, becoming, it fellowed hereupon and with the aesthetics idea which pursues the quality of change and life of our dietary life there is to a culture and the repast tool which is an essential width enjoyed from meaning as the jar tool and with meaning as the object and it will be able to appreciate it developed it came. The research which it sees the repast fool of the Orient and the West it is appropriate from dietary life and it uses to respect with It observes the understanding of the chopsticks and the cutlery. With the repast tool development and evolution or it undergoes the process of change and comparison analyzes becomes the all chopsticks and the cutlery with each other and the understanding for the culture collision and a culture harmony of the heterogeneous element which bisection is objective of research.

• 한국융합학회논문지
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• 제9권11호
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• pp.491-500
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• 2018

병원간호사가 인식하는 간호조직문화의 유형을 파악하고, 이러한 간호조직 문화와 전문적 삶의 질과의 관계를 확인하기 위함이다. 대상자는 충북의 C시에 소재한 일개 대학병원에 근무하는 간호사 216명이다. 자료 분석은 SPSS/WIN22.0 프로그램을 이용하여 분석하였다. 전문직 삶의 질의 하위영역인 공감만족에 미치는 영향요인으로는 혁신지향문화, 결혼상태, 관계지향문화가 유의한 예측변인으로 나타났고, 이차성 외상 스트레스는 연령, 업무지향문화, 위계지향문화가 유의한 예측변인으로 나타났으며, 소진은 관계지향문화, 임상경력, 위계지향문화가 유의한 예측변인으로 나타났다. 이 같은 결과를 토대로 조직 내 혁신지향문화 및 관계지향문화 형성을 위한 간호사들의 인식 전환 및 개선을 위한 중재프로그램 개발 및 임상에서의 효과를 검정하는 연구가 필요하며, 숙련되고 임상경험이 풍부한 우수한 간호사를 확보하기 위해 조직차원에서의 근무환경 및 처우 개선이 이루어져야 한다.

• 한국콘텐츠학회논문지
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• 제18권10호
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• pp.545-557
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• 2018

본 연구는 문화예술관람이 자아존중감과 삶의 만족도에 어떠한 영향을 미치는가에 대해 살펴보고자 하였다. 구체적으로는 문화예술관람이 자아존중감에 미치는 영향을 규명하고, 문화예술관람이 삶의 만족도에 미치는 영향을 규명하고자 하였으며, 자아존중감이 삶의 만족도에 미치는 영향을 규명하고자 하였다. 또한, 문화예술관람이 자아존중감의 매개를 통해 삶의 만족도에 미치는 간접효과가 유의한가에 대해 살펴보고자 하였다. 본 연구의 목적을 달성하기위하여 서울특별시, 대구광역시, 부산광역시에 각 250부의 설문지를 배포하여 할당 표본 추출을 실시하였다. 설문조사기간은 2018년 6월 18일부터 6월 30일까지 13일 동안 설문조사를 실시하였고, 서울지역에서 224부 회수, 대구지역에서 181부 회수, 부산지역에서 199부가 회수되어 전체 604부를 최종분석에 사용하였다. 분석결과, 문화예술관람을 많이 할수록 자아존중감을 향상시키는 것으로 나타났고, 문화예술관람을 많이 할수록 삶의 만족도도 유의하게 향상되는 것으로 나타났으며, 자아존중감이 높아질수록 삶의 만족도가 향상되는 것으로 나타났다. 아울러, 문화예술관람을 많이 할수록 자아존중감의 매개를 통해 삶의 만족도가 향상되는 간접효과도 유의한 것으로 나타났다.