한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권1호
  • /
  • Pages.10-17
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  • 2023
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Comparative Evaluation of Culture Media for Quantification of Lactic Acid Bacteria in Various Dairy Products

  • Eiseul Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Shin-Young Lee (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Yoon-Soo Gwak (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hyun-Jae Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Ik-Seon Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hyo-Sun Kwak (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hae-Yeong Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University)
  • 투고 : 2023.01.25
  • 심사 : 2023.03.14
  • 발행 : 2023.03.28
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초록

Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 107 colony-forming unit (CFU)/ml for fermented milk products and 108 CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

키워드

과제정보

This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01662001)" Rural Development Administration. Republic of Korea.

참고문헌

  1. Hatti-Kaul R, Chen L, Dishisha T, Enshasy H El. 2018. Lactic acid bacteria: From starter cultures to producers of chemicals. FEMS Microbiol. Lett. 365. doi: 10.1093/femsle/fny213.
  2. Giraffa G. 2021. The microbiota of grana padano cheese. A review. Foods 10: 2632.
  3. Yamamoto E, Watanabe R, Ichimura T, Ishida T, Kimura K. 2021. Effect of lactose hydrolysis on the milk-fermenting properties of Lactobacillus delbrueckii ssp. bulgaricus 2038 and Streptococcus thermophilus 1131. J. Dairy Sci. 104: 1454-1464. https://doi.org/10.3168/jds.2020-19244
  4. Kim E, Cho EJ, Yang SM, Kim HY. 2021. Identification and monitoring of Lactobacillus delbrueckii subspecies using pangenomicbased novel genetic markers. J. Microbiol. Biotechnol. 31: 280-289. https://doi.org/10.4014/jmb.2009.09034
  5. Kim J, Lee MH, Kim MS, Kim GH, Yoon SS. 2022. Probiotic properties and optimization of gamma-aminobutyric acid production by Lactiplantibacillus plantarum FBT215. J. Microbiol. Biotechnol. 32: 783-791. https://doi.org/10.4014/jmb.2204.04029
  6. Mehra A. 2022. Probiotics Market. by Application (Functional Food & Beverages (Dairy Products, Non-dairy Beverages, Infant Formula, Cereals), Dietary Supplements, Feed), Ingredient (Bacteria, Yeast), Form (Dry, Liquid), End User, & Region - Global Forecast to 2026.
  7. Kim E, Kim D, Yang SM, Kim HY. 2022. Validation of probiotic species or subspecies identity in commercial probiotic products using high-resolution PCR method based on large-scale genomic analysis. Food Res. Int. 154: 111011.
  8. Patro JN, Ramachandran P, Barnaba T, Mammel MK, Lewis JL, Elkins CA. 2016. Culture-independent metagenomic surveillance of commercially available probiotics with high-throughput nextgeneration sequencing. mSphere 1: e00057-16.
  9. Ashraf R, Shah NP. 2011. Selective and differential enumerations of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium spp. in yoghurt - A review. Int. J. Food Microbiol. 149: 194-208. https://doi.org/10.1016/j.ijfoodmicro.2011.07.008
  10. Sehrawat R, Abdullah S, Khatri P, Kumar L, Kumar A, Mujumdar AS. 2022. Role of drying technology in probiotic encapsulation and impact on food safety. Dry. Technol. 40: 1562-1581. https://doi.org/10.1080/07373937.2022.2044844
  11. Kim B-Y. 2019. Recent research technologies for quality control of commercial probiotics. Curr. Top. Lact. Acid Bact. Probiotics 5: 39-46. https://doi.org/10.35732/ctlabp.2019.5.2.39
  12. Davis C. 2014. Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria. J. Microbiol. Methods 103: 9-17. https://doi.org/10.1016/j.mimet.2014.04.012
  13. Hayek SA, Gyawali R, Aljaloud SO, Krastanov A, Ibrahim SA. 2019. Cultivation media for lactic acid bacteria used in dairy products. J. Dairy Res. 86: 490-502. https://doi.org/10.1017/S002202991900075X
  14. Champagne CP, Ross RP, Saarela M, Hansen KF, Charalampopoulos D. 2011. Recommendations for the viability assessment of probiotics as concentrated cultures and in food matrices. Int. J. Food Microbiol. 149: 185-193. https://doi.org/10.1016/j.ijfoodmicro.2011.07.005
  15. Sule J, Korosi T, Hucker A, Varga L. 2014. Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria. Braz. J. Microbiol. 45: 1023-1030. https://doi.org/10.1590/S1517-83822014000300035
  16. Zhao Y, Yu L, Tian F, Zhao J, Zhang H, Chen W, et al. 2021. An optimized culture medium to isolate: Lactobacillus fermentum strains from the human intestinal tract. Food Funct. 12: 6740-6754. https://doi.org/10.1039/D1FO00209K
  17. Bujalance C, Jimenez-Valera M, Moreno E, Ruiz-Bravo A. 2006. A selective differential medium for Lactobacillus plantarum. J. Microbiol. Methods 66: 572-575. https://doi.org/10.1016/j.mimet.2006.02.005
  18. Tharmaraj N, Shah NP. 2003. Selective enumeration of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, and propionibacteria. J. Dairy Sci. 86: 2288-2296. https://doi.org/10.3168/jds.S0022-0302(03)73821-1
  19. Kim Y-S, Hwang S-I, Kim S-T, Han N-E, Kim H-Y, Kim H-S, et al. 2018. Investigation of the lactic acid bacteria content of probiotic and lactic acid bacteria products: A study on changes in the preservation method of probiotic products. J. Food Hyg. Saf. 33: 474-482. https://doi.org/10.13103/JFHS.2018.33.6.474
  20. Dave RI, Shah NP. 1996. Evaluation of media for selective enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, and Bifidobacteria. J. Dairy Sci. 79: 1529-1536. https://doi.org/10.3168/jds.S0022-0302(96)76513-X
  21. Van de Casteele S, Vanheuverzwijn T, Ruyssen T, Van Assche P, Swings J, Huys G. 2006. Evaluation of culture media for selective enumeration of probiotic strains of lactobacilli and bifidobacteria in combination with yoghurt or cheese starters. Int. Dairy J. 16: 1470-1476. https://doi.org/10.1016/j.idairyj.2005.12.002
  22. Franca de Souza V, Luis Ramirez Ascheri J, Palmiro Ramirez Ascheri D. 2019. Characterization and potential application of blend of passion fruit peel with rice flour in an extruded product for fiber enhancement. J. Food Nutr. Res. 7: 522-529. https://doi.org/10.12691/jfnr-7-7-6
  23. Kim E, Yang SM, Cho EJ, Kim HY. 2022. Evaluation of matrixassisted laser desorption/ionization time-of-flight mass spectrometry for the discrimination of Lacticaseibacillus species. Food Microbiol. 107: 104094.
  24. Roy D. 2001. Media for the isolation and enumeration of bifidobacteria in dairy products. Int. J. Food Microbiol. 69: 167-182. https://doi.org/10.1016/S0168-1605(01)00496-2
  25. Nebra Y, Blanch AR. 1999. A new selective medium for Bifidobacterium spp. Appl. Environ. Microbiol. 65: 5173-5176. https://doi.org/10.1128/AEM.65.11.5173-5176.1999
  26. Lee HM, Lee Y. 2008. A differential medium for lactic acid-producing bacteria in a mixed culture. Lett. Appl. Microbiol. 46: 676-681. https://doi.org/10.1111/j.1472-765X.2008.02371.x
  27. Lin WH, Hwang CF, Chen LW, Tsen HY. 2006. Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food Microbiol. 23: 74-81. https://doi.org/10.1016/j.fm.2005.01.013
  28. Oh Y, Lee MS, Ahn Y-J, Ahn J-H. 2015. Application of measurement method for lactic acid bacteria content on coccus including yoghurt. Curr. Top. Lact. Acid Bact. Probiotics 3: 34-36.  https://doi.org/10.35732/ctlabp.2015.3.1.34