• International Journal of Industrial Entomology and Biomaterials
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• 제35권1호
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• pp.58-62
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• 2017

The two-spotted spider mite (Tetranychus urticae) has sustained damage on more than 200 host plants worldwide. Many farmers have relied on chemical acaricides to control mite, but the abuse of acaricides has caused serious resistance to mite. To overcome this problem, microbial control using entomopathogenic fungi have been studied. Entomopathogenic fungi have been an important role against the control of pest, and most of their culture products have been demonstrated to have virulence against pest population. In this study, we evaluated and compared the virulence of culture filtrates, aerial conidia and blastospores of selected Metarhizium anisopliae 4-2 and Beauveria bassiana 2R-3-3-1, respectively, among two-spotted spider mite-pathogenic fungi. As a result, the virulence was confirmed in all treatments, and the accumulated mortality rates were between 77 and 100% within 7 days. Especially, treatment with the fungal culture filtrate alone exhibited quite high virulence, and combined treatment with aerial conidia or blastospores enhanced activity. However, the median lethal time of treatments was not significantly different. When two isolates were compared, M. anisopliae 4-2 showed higher virulence than B. bassiana 2R-3-3-1. These results suggest that the selected two fungal isolates and their culture products could be used effectively for the control of two-spotted spider mite.

• Reproductive and Developmental Biology
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• 제38권3호
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• pp.93-98
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• 2014

The objective of this study was to investigate the changes of oxidative stress and antioxidant enzyme during in vitro development with washing culture oil in porcine embryos. During the culture, the four types of culture oil such as paraffin oil with or without washing and mineral oil with or without washing were examined. The oil was washed with PZM-3 during 7 days and collected oil only. The embryos were stained with CellTracker$^{TM}$ Red, DCFDA and Hoechst 33342 to confirm the effects of the oil. As a results, Cleavage rates and total cell number were no difference among the four oil groups. However, ${\geq}16$ cell embryos were significantly different in fore type oil treatment and blastocyst rate was significantly higher washing paraffin treatment than in other group(p<0.05). Also, the expression of free radical were lower in washing paraffin oil than in other groups (p<0.05). On the other hand, the expression of glutathione were not significant different among paraffin oil with or without washing and mineral oil with or without washing, however washing paraffin oil and washing mineral groups were higher than other treatment groups. In conclusion, the washing oil was expected with positive effects on in vitro development in porcine embryos.

• 품질경영학회지
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• 제47권4호
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• pp.861-873
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• 2019

Purpose: The purpose of this study is to investigate the effects of organizational culture, personality Traits, work addiction and workplace family conflict on the life satisfaction of Korean Air crew. Methods: The collected data through the survey were analyzed using structure equation model analysis The sample used for the empirical analysis is a total of 578 questionnaires. Results: Empirical analysis summarized next. Organizational culture and personality characteristics have some effects on work poisoning. However, the compulsion of work driven does not affect work-family conflict, Family-Work Conflict does not affect Life Satisfaction. Conclusion: This paper has a contribution in that it extends the range of the academic research concerning Workaholic. Also this paper provides implications on effective human resource management method suitable for airline industry.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.187.3-187
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• 2005

• 지역과문화
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• 제7권3호
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• pp.1-21
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• 2020

생활에 밀착한 일상 속 문화 활동을 강조하는 생활문화는 예술과 일상의 통합과 타자와 소통하는 공동체적 삶을 지향하고 이 과정에서 개인과 사회가 가져야할 가치들을 환기시키고 회복한다. 본 연구는 이렇게 지역문화로서 생활문화의 가치가 발현되는 개별적이고 복잡한 맥락과 과정을 이해하기 위하여, 수원 지역의 '고색전통농악보존회' 생활문화 활동 사례를 분석한다. 이를 통해 실제 현장에서 예술과 일상이 어떻게 통합되며 공동체적 삶을 회복시키고 있는지 진단한다. 문헌자료와 참여관찰, 인터뷰를 통한 현상학적 연구를 수행하여 개인, 공동체, 지역영향으로 생활문화 활동과 공적지원의 사회적 영향을 범주화하였다. 연구결과 생활문화 공적 지원으로 늘어난 활동경험은 공동체의 예술적 발전과 인적 상호작용을 활성화시키고 더불어 개인은 예술적 본성을 찾고 지역사회에 영향을 주는 문화예술의 주체이자 실천가로 거듭나고 있었다. 또한 지역주민도 함께 참여하는 생활문화 활동의 과정에서 참여자들의 지역사회 일원으로서의 정체성이 강화되고 있음을 확인하였다.

• 한국가정과교육학회지
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• 제23권4호
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• pp.1-17
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• 2011

본 연구는 청소년의 전통 의생활 문화인식을 증진시키기 위하여 의생활 단원 내용을 중심으로 교수 학습 과정안을 개발하고 적용하였다. 7차 교육과정 고등학교 1학년 교과서 12종과 7차 개정교육과정을 분석하고 이를 바탕으로 '가정생활문화 변화'단원을 중심으로 '전통문화', '한복의 변천사', '한복의 색채', '한복의 문양과 소재', '한복의 세계화' 5개의 학습 주제를 선정하였다. 선정한 주제에 대해, 5차시의 교수 학습 과정안 및 48종의 수업자료(학생자료 22개, 수업자료 22개, 교사 자료 4개)를 개발하고 수업에 적용하였다. 청소년들의 전통의생활 문화에 대한 인식도는 사전검사 평균에 비해 사후 검사 평균이 높게 나타난 것으로 보아 본 연구의 교수 학습 과정안이 전통 의생활 전통문화에 대한 올바른 인식, 외래문화에 대한 수용, 전통 문화에 대한 긍정적 자세 등 전통 문화 인식 고취에 효과적이었다고 할 수 있다. 전통의생활 교육에 대한 인식 또한 유의미하게 향상되어 전통 문화교육의 중요성을 알 수 있었다. 이에 본 연구자는 의생활 문화 교육과 관련하여 더 다양한 전통문화 관련 주제가 개발되어야 하며 더 강화되어야 한다고 제언하는 바이다. 뿐 아니라 식생활, 주생활 분야에도 전통문화 교수 학습 과정안이 개발되고 적용되는 등 다양한 전통문화 계승 교육 연구가 이루어질 필요가 있다.

• 한국발생생물학회지:발생과생식
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• 제26권1호
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• pp.13-21
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• 2022

Neurokinin B (NKB) is a neuropeptide involved in the regulation of reproductive endocrine system of vertebrate animals, including fish. However, the pathway of NKB action in fish has not been clearly elucidated. In order to clarify the effect of NKB and NKF (neurokinin F) on gonadotropic hormone (GTH) gene expression in the pituitary, we studied the changes of LHβ and FSHβ gene expressions by using two different pituitary culture methods (whole pituitary culture or dispersed pituitary cell culture). Pituitaries were removed from mature female and male Nile tilapia. Changes of LHβ and FSHβ gene expressions were measured and compared after the treatment with NKB or NKF peptides at concentrations 0 to 1,000 nM. Expression of GTH genes in the whole pituitary cultures treated with NKB or NKF peptides did not show significant difference except in female at one concentration when treated with NKF. On the contrary, there were significant changes of GTH gene expressions in the dispersed pituitary cell cultures when treated with NKB and NKF peptides. These results suggest that dispersed pituitary cell culture is more relevant than whole pituitary culture in studying the function of pituitary, and that NKB and NKF could act directly on the pituitary to regulate the expression of GTH genes.

• Reproductive and Developmental Biology
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• 제38권2호
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• pp.85-91
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• 2014

Despite many researches related with in-vitro culture of porcine spematogonial stem cells (SSCs), adherent culture system widely used has shown a limitation in the maintenance of porcine SSC self-renewal. Therefore, in order to overcome this obstacle, suspension culture, which is known to have numerous advantage over adherent culture, was applied to the culture of porcine SSCs. Porcine SSCs retrieved from neonatal testes were suspension-cultured for 5 days or 20 days, and characteristics of suspension-cultured porcine SSCs including proliferation, alkaline phosphatase (AP) activity, and self-renewal-specific gene expression were investigated and compared with those of adherent-cultured porcine SSCs. As the results, the suspension-cultured porcine SSCs showed entirely non-proliferative and significantly higher rate of AP-positive cells and expression of self-renewal-specific genes than the adherent-cultured porcine SSCs. In addition, long-term culture of porcine SSCs in suspension condition induced significant decrease in the yield of AP staining-positive cells on post-day 10 of culture. These results showed that suspension culture was inappropriate to culture porcine SSCs, because the culture of porcine SSCs in suspension condition didn't stimulate proliferation and maintain AP activity of porcine SSCs, regardless of culture periods.

• 복식문화연구
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• 제21권2호
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• pp.301-308
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• 2013

The purpose of this research is to classify fashion consumers based on their attitudes, goals and values of life. It is to understand what drives human behaviors as well as to learn the various directions people live in society using Bangkok people as the samples. Online and on-site questionnaire survey is employed. Questions are designed to focus on 7 aspects of life, ranging from private life, professional life, social life, politics, culture and information interaction, household business and finance, and consumption of products and services in the main market. The research results can be used to classify consumers' lifestyles into 20 major and numerous minor groups of lifestyle. Lifestyles of male and female samples are compared to investigate their different patterns and directions. Fashion trend diagram is used to analyze the overlapped lifestyles of mass consumers. The lifestyle segmentations would benefit to designer and fashion branding team in understanding their target group deeper inside the background of their behaviors.

• 한국식생활문화학회지
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• 제11권2호
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• pp.179-206
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• 1996

Generally, the culture of dietary life in a country is closely related to the heritage of racial culture, and it is very important to inherit and develop the culture of the traditional dietary life. This research is to investigate consciousness and behaviors of dietary life using a questionnaire survey for 521 housewives living in Seoul and Kyonggido. The data was analyzed with frequency, percentage, T-test, $X^2$ (Chi-square)-test, dispersion analysis, Pearson's correlation and hierarchical multiple-regression analysis. The results of the survey are summarized as follows; 1. Consciousness of Traditional Foods. They thought that traditional foods were Sinsollo, sorts of beverage, Dduk (Korean cake), Kim-chi, Hankwa (Korean cookies), and Kujolpan, and that traditional foods were proud for its various tastes. But they thought the cuisine of the traditional food is very complicated, accordingly the frequency of traditional food was decreased. Therefore they thought that its tastes was important to develop and spread traditional foods. 2. Dietary Life Behaviors for Traditional Food. They take traditional foods on big holidays and religious service days. It is their mother who taught them the traditional cooking method. Most ftequently used cooking method is boiling. They gave their children a traditional snack such as 'Dduk (Korean cake), noodles, and fried food. Frequency of taking beverage, Hankwa (Korean cookies), Juk (guel) and Dduk (Korean cake) have been decreased. 3. The Correlation of General Environment and Consciousness, Knowledge, Dietary Life Behaviors of Traditional food. The older they are, the more eager to teach traditional food cooking, to preserve the traditional table setting, to give their children traditional snack and the more traditional dietary life behaviors are.