• Title/Summary/Keyword: library 3.0

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Meta-Analysis of the Effectiveness on Foot-Reflexo-Massage for Cancer Patients (암환자에게 적용한 발반사 마사지 중재효과의 메타분석)

  • Kim, Min-Young;Oh, Pok-Ja
    • Asian Oncology Nursing
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    • v.11 no.2
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    • pp.127-135
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    • 2011
  • Purpose: This study was performed to analyze the characteristics and effect sizes of intervention studies on foot-reflexo-massage applied to cancer patients. Methods: For meta-analysis, a total of 159 studies were retrieved from search engines such as RISS, nanet, KISS, richis and KoreaMed. 16 studies published from 1990 to 2010 were selected based on the inclusion criteria. The data were analyzed with the RevMan 5.0 program of Cochrane library. Results: 1) The mean score of 1 implement time on foot-reflexo-massage was 25.62 minutes, the average number of days was 4.12 days, and the total number of average intervention frequency was 4.25 times. 2) Intervention studies on foot-reflexo-massage included 9 studies on anxiety (56.3%), 7 for pain (43.8%), 5 for BP/pulse (31.3%), 5 for fatigue (31.3%), 3 for nausea/vomiting (18.8%), 3 for sleep satisfaction (18.8%), and 2 for depression (12.5%). 3) The effect sizes of the intervention studies that showed higher effect size were in order, anxiety (d=-1.76), fatigue (d=-1.43), depression (d=-1.03), nausea and vomiting (d=-0.83), pain (d=-0.77), pulse rate (d=-0.61), blood pressure (d=-0.55), and sleep satisfaction (d=0.43). Conclusion: This study suggests that foot-reflexo-massage can increase sleep satisfaction, whereas decreasing blood pressure, pulse rate, anxiety, fatigue, depression, nausea, vomiting and pain.

Construction of cDNA Library for Using Virus-induced Gene Silencing (VIGS) Vector with the Sweetpotato Whitefly, Bemisia tabaci(Hemiptera: Aleyrodidae) (담배가루이(Bemisia tabaci, Aleyrodidae, Hemiptera)에서 Virus-induced Gene Silencing (VIGS) Vector를 이용하기 위한 cDNA Library 제작)

  • Ko, Na Yeon;Lim, Hyoun Sub;Yu, Yong Man;Youn, Young Nam
    • Korean journal of applied entomology
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    • v.54 no.2
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    • pp.91-97
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    • 2015
  • The sweetpotato whitefly, Bemisia tabaci, is the major insect pest that transmitted over 100 plant viruses including tomato yellow leaf curl virus (TYLCV) of tomato plant as virus vector in the world. In this study, cDNA library of whitefly was constructed using Gateway system for selecting target gene in order to control of B. tabaci using virus-induced gene silencing (VIGS) vector with RNAi. First of all, when using oligo d(T) rimer, the calculated titer of cDNA library was confirmed with $1.4{\times}10^4$ clones and average insert sizes was confirmed with 1 kb. However, insert size was very big for construction of cDNA. Otherwise, when using attB-N25 random primer and sonication for 6 sec, the calculated titer of cDNA library was confirmed with $1.04{\times}10^5$ clones. But mostly insert band wasn't identified on the electrophoresis, because it seemed that insert size is too small (${\leq}100bp$), also the size of identified insert was somewhat big. Finally, when using oligo d(T) primer and sonication for 1 sec, cDNA insert of whitefly was appropriated for VIGS with 300-600 bp. However, cDNA sequence included a poly A and titer was very low to $5.2{\times}10^2$ clones. It was supposed that heat shock transformation was used instead of electro-transformation. It is considered that when constructing cDNA library for using VIGS vector, (1) random primer should be used for First strand cDNA synthesis in order to remove poly A and (2) sonication for 1 sec should be performed in order to get appropriated insert size and (3) electro-transformation should be performed in order to improve transformation efficiency.

Quality Control of Web Information Service: the case of government web site (정보서비스 평가연구: 국내 행정부처 Web 정보사례)

  • Yoo, Sa-Rah
    • Journal of the Korean Society for Library and Information Science
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    • v.35 no.3
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    • pp.93-115
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    • 2001
  • Evaluating information quality of government Web service, applying quality measurement methods for web - database was conducted. This approach intended to control the quality of information service by indicating the problems and weak points in terms of retrieval, use of easiness, and user support, and by suggesting the guidelines for improvement. There were two main results to most of the investigation. The first one indicated that web information service of eighty-two percent of domestic government sites was not good enough (less than middle score). and only eight percent of them got higher score than middle point. The second result was that user support aspect was the worst part that we need to consider future course of action for service improvement. More specific guidelines were summarized at the discussion section.

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A Design of Programmable Fragment Shader with Reduction of Memory Transfer Time (메모리 전송 효율을 개선한 programmable Fragment 쉐이더 설계)

  • Park, Tae-Ryoung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.12
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    • pp.2675-2680
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    • 2010
  • Computation steps for 3D graphic processing consist of two stages - fixed operation stage and programming required stage. Using this characteristic of 3D pipeline, a hybrid structure between graphics hardware designed by fixed structure and programmable hardware based on instructions, can handle graphic processing more efficiently. In this paper, fragment Shader is designed under this hybrid structure. It also supports OpenGL ES 2.0. Interior interface is optimized to reduce the delay of entire pipeline, which may be occurred by data I/O between the fixed hardware and the Shader. Interior register group of the Shader is designed by an interleaved structure to improve the register space and processing speed.

Isolation of Deletion Mutants by Reverse Genetics in Caenorhabditis elegans

  • Park, Byung-Jae;Lee, Jin ll;Lee, Jiyeon;Kim, Sunja;Choi, Kyu Yeong;Park, Chul-Seung;Ahn, Joohong
    • Animal cells and systems
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    • v.5 no.1
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    • pp.65-69
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    • 2001
  • Obtaining mutant animals is important for studying the function of a particular gene. A chemical mutagenesis was first carried out to generate mutations in C. elegans. In this study, we used ultraviolet-activated 4,5',8-trimethylpsoralen to induce small deletion mutations. A library of mutagenized worms was prepared for recovery of candidate animals and stored at $15^{\circ}C$ during screening instead of being made into a frozen stock library. In order to isolate deletion mutations in target genes, a polymerase chain reaction (PCR)-based screening method was used. As a result, two independent mutants with deletions of approximately 1.0 kb and 1.3 kb were isolated. This modified and improved reverse genetic approach was proven to be effective and practical for isolating mutant animals to study gene function at the organismal level.

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An analytical study on the major Korean periodical indexes (한국의 현행색인지에 관한 분석적 연구)

  • 이절자;박준식
    • Journal of Korean Library and Information Science Society
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    • v.24
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    • pp.365-389
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    • 1996
  • This study attempts to analyze the major periodical indexes and catalogs of 231 titles published in Korea in the form of monographs from 1973 to 1993, in order to identify the trends of their publications and the problems in its compilation and to suggest an idea for the improvements. The results of this can be summarized as follow : 1. The publication of indexes are concentrated on some subject fields including those of social science. And there should be general indexes in the fields of the humanities and pure sciences for the researches in these fields to consult. 2. The titles of the indexes are represented by various forms. 3. The materials included are extended to all types of them including monographs, serials, these and dissertations, and newspapers, so that enhances the usefulness of the subject indexes. 4. Most of indexes are published in quarterly or yearly and seldom publish the cumulative editions. 5. Most of indexes(140 titles, 90%) are classified indexes. 6. The descriptions include basic elements of author, title, translator, contributed title, issue number, date, pages, etc, in the case of most of indexes. 7. Cross reference are seldom used, because most of indexes are classified ones. 8. Auxiliary indexes or a n.0, ppendixes are not included in 34 titles of 21%.

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A Reform about the KCR 4th ed.(Draft) (韓國目錄規則 4版(草案)에 대한 提言)

  • 김자후
    • Journal of Korean Library and Information Science Society
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    • v.33 no.3
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    • pp.235-256
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    • 2002
  • The purpose of this study is to suggest a reform after detecting out issues and problems associated with the KCR 4th ed.(draft). KCR(Korean Cataloguing Rules) is representative cataloguing rules in Korea, the 4th ed.(draft) of which was prepared by Cataloguing Committee(department of KLA) in 2001. As a result of this study, a lot of problem which are glossary, context, inconsistency of the contents, rules applied to card catalog, and others, were found out. Thus, substantial improvements were able to be suggested.

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Cloning of the Alkaline Phosphatase Gene from Kluyveromyces fragilis

  • Kim, Jong-Guk;Hwang, Seon-Kap;Kwon, Kaeg-Kyu;Nam, Joo-Hyun;Hong, Soon-Duck;Seu, Jung-Hwn
    • Journal of Microbiology and Biotechnology
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    • v.2 no.4
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    • pp.237-242
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    • 1992
  • In order to clone the gene coding for alkaline phosphatase in the yeast Kluyveromyces fragilis, a genomic library was constructed using the yeast-E. coli shuttle vector pHN114 as a cloning vector. From the genomic library, a clone carrying the gene was isolated and the plasmid was designated as pSKH101. A restriction enzyme map was made using this plasmid. Subcloning experiments and complementation studies showed that alkaline phosphatase was active only in the original 3.1 kb insert. Southern hybridization analysis confirmed that the cloned DNA fragment was derived from K. fragilis genomic DNA. Using a minicell experiment, the product of the cloned gene was identified as a protein with a molecular weight of 63 KDa. A 0.6 kb HindIII fragment, which showed promoter activity, was isolated using the E. coli promoter-probe vector pKO-1.

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Identification of Novel Esterase from Metagenomic Library of Yangtze River

  • Wu, Chao;Sun, Baolin
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.187-193
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    • 2009
  • A metagenomic library of surface-water microbes from the Yangtze River in China was constructed, and a novel esterase, designated as EstY, was isolated and characterized. EstY had 423 amino acids with an estimated molecular mass of 44 kDa and pI of 7.28. It hydrolyzed various p-nitrophenyl esters(acetate, butyrate, caprate, caprylate, laurate, myristate, and palmitate) and its best substrate was p-nitrophenyl caprate(C8). The optimum pH for EstY activity was 9.0 and the optimum temperature was $50^{\circ}C$. Metal ions, such as $Mn^{2+},\;Co^{2+},\;Hg^{2+},\;Zn^{2+},\;and\;Fe^{3+}$, strongly inhibited the activity of EstY, whereas $Mg^{2+}$ was required for maximal activity. Activity remained in the presence of 10% alcohol, acetone, isopropanol, and dimethyl sulfoxide, respectively. An analysis of the amino acid sequence deduced from estY revealed that it had 7 closely related lipolytic enzymes. Moreover, a sequence analysis showed that EstY, like its 7 relatives, did not belong to any known lipolytic enzyme family.

A Study on the Paper & Muju Characters(武周字) on which the Mugujongkyong from the Sokgat'ap (석가탑 "무구정경"바탕종이 및 무주자의 사용 문제)

    • Journal of Korean Library and Information Science Society
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    • v.30 no.3
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    • pp.323-344
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    • 1999
  • First, having noticed that the paper on which the Mugujongkyong from the Sokgat'ap was Broussonetia Kazinoki Siebold, produced under the Tochim process, it is confirmed that the paper used for printing the Mugujongkyong form the Sokgat'ap was Broussonetia Kazinoki Siebold produced in Shilla, by introducing the common fact that the papers on which : written in 755 A.D.) and in Soochung-sokt'ap Hwaomasa were written were the same paper fabricated by the Tochim process. Therefore it is proved that the printing place of the Mugujongkyong from the Sokgat'ap was also Kyongju region in Shilla. Furthermore it is proved that the year of storing Broussonetia kazinoki Siebold n which tarani of the Mugujongkyong was written was circa 705 A.D., provin12g that Broussonetia Kazinoki Siebold was in production in Shilla in 8th Century. Also by confirming the fact that very thin -- mere 0.019mm thick -- Broussonetia Kazinoki Siebold was in production in Shilla in 8th Century, it is proved that Shilla has ability to produce Broussonetia Kazinoki Siebold for Woodblock printing purpose. Second, concerning the Muju Characters written in the ugujongkyong from the Sokgat'ap, mixed use of the Muju Characters was noticeable in written in 755 A.D. from the Sooch'ungsokt'ap at the Hwaomsa written in the same era and the Tripitaka Koreana in Japan was printed in 690's A.D. because of appearance of the Muju Characters in the Saddhamapun-darika is erroneous.

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