• Asian-Australasian Journal of Animal Sciences
• /
• 제15권3호
• /
• pp.321-325
• /
• 2002

Karyotype analysis was carried out on blood samples of 30 water buffaloes belonging to different breed groups (i.e. Philippine Carabao (PC), Indian Murrah (IM), Bulgarian Murrah (BM), "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC" and "75% IM-25% PC"), using the modified Leucocyte Culture Technique. The modal chromosome numbers of the PC, "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC", IM, BM and "75% IM-25% PC" were 2n=48, 49, 49, 50, 50 and 50, respectively. The water buffalo chromosomes are mostly acrocentric (79.67%) and the remainder submetacentric (20.33%). Results of the ordinary least square analysis showed significant breed effects (p<0.01) on other karyotypic characteristics (i.e. relative length, arm ratio and centromeric index). Significant correlation between karyotypic characteristics and some animal performance traits were also found. The significant correlation values imply that karyotypic characteristics can be used as important criteria to select potentially productive young water buffaloes. In the future, more production and reproduction traits from non-institutional herds should be included in the analysis to reveal meaningful correlations with various karyotypic characteristics.

• 한국임상수의학회지
• /
• 제7권2호
• /
• pp.439-450
• /
• 1990

This study was performed to investigate the effects of Aloe vera administration(500mg/kg. B.W., s.i.d., P.O.) on hematology, blood chemical values and histopathological changes of spleen in cobalt-60 gamma Irradiated mice. 1. Total leucocyte counts of aloe administered groups were less decreased and more rapidly returned to normal level than those of control group. 2. Lymphocyte counts were rapidly decreased on the 1st day after irradiation in all experimental groups, but the counts were less decreased in aloe administered groups than those of control group. 3. Erythrocyte counts, hemoglobin values and hematocrit values of aloe administered groups were maintained within normal level, but these values showed lower values on the 14th day after irradiation in control group then returned to normal level on the 21th day after irradiation. 4. There were no differences between aloe administered groups and control group In LDH, SGOT and glucose values. 5. Histopathological changes of spleen in aloe administered groups showed less lymphocytic necrosis and depletion than those of control group.

• Asian-Australasian Journal of Animal Sciences
• /
• 제14권9호
• /
• pp.1304-1307
• /
• 2001

The laying performance of Japanese quails fed graded levels of high glucosinolate ($92.5{\mu}mole/g$) rapeseed meal (RSM) was assessed. One hundred and twenty Japanese quails aged 1 day-old were assigned at random to four dietary treatments consisting of 0, 50, 75 or 100 g/kg RSM in the diet replacing part of the soybean meal and de-oiled rice bran in a standard quail ration. 12 female representative quails from each diet were selected at random and housed in individual cages from 7-20 wk of age. The egg production, feed intake and FCR was comparable among the different dietary groups. The egg quality characteristics, organoleptic evaluation of boiled eggs as well as the haematological (haemoglobin, total erythrocyte count, total leucocyte count) and biochemical (glucose, protein, cholesterol, aspartate amino transferase, alanine amino transferase and alkaline phosphatase) constituents did not differ significantly among the groups. The gross and histopathological studies of vital organs did not reveal any appreciable changes. The feed cost was reduced by the incorporation of RSM in the diet, but only the production cost of quails fed the 75 g/kg RSM was lower in comparison to other groups. In the present study, the laying potential of Japanese quail was well-maintained up to the 100g/kg dietary level of rapeseed meal.

• 대한한의학회지
• /
• 제17권2호
• /
• pp.178-190
• /
• 1996

This experiment was performed in order to study the effects of Haepyoyijintang on the pulmonary injury caused by $SO_2$. Healthy adult male rats weighting about 250g were divided into 4 groups- the Normal group, the Control group, the group of Haepyoyijintang administration for 5 days after $SO_2$ gas exposure (Sample I ), and the group of Haepyoyijintang administration for 10 days before and for 5 days after $SO_2$ gas exposure (SampleⅡ). The results were obtained as follows; 1. In the total number of leucocyte, Sample I and Sample II decreased significantly compared with Control group. 2. In the erythrocyte sedimentation rate, Sample I and Sample Ⅱ decreased significantly compared with Control group. 3. In the lung weight, Sample I and Sample II decreased significantly compared with Control group. 4. In the lung TBA level, only Sample II decreased significantly compared with Control group. 5. In the tracheal glycoprotein level, Sample I and Sample II decreased significantly compared with Control group. According to the above results, Haepyoyijintang has significant effects on the pulmonary injury caused by $SO_2$ in rats.

• KSBB Journal
• /
• 제6권1호
• /
• pp.45-54
• /
• 1991

The aims of this study were investigated the antitumor effects and immunological activities of lipopolysaccharides (LPS) extracted from Proteus vulgaris RH-90 toward sarcoma-180 cells. LPS extracted from Proteus vulgris RH-90 was irradiated with gamma ray for detoxification. The tumor incidence of sarcoma-180 occurs all group which injected with gamma ray irradiated LPS and tumor of sarcoma-180 was necrotized with breeding in the injected group of l0$\mu\textrm{g}$ LPS. The inhibition ratio of tumor growth showed at the highest level of 60.88% when 5$\mu\textrm{g}$ gamma ray irradiated LPS was injected into mice. The prolongation ratio of life showed 20.72% when injected into mice with gamma ray irradiated LPS of 5$\mu\textrm{g}$. In the effect of immunological activity, the number of circurating leucocyte and peritoneal exudate cells were increased significantly in the treatment group than that control group, and dose-dependent response indicated by the increase of weights of immunorgans which revealed the improvement of immunity. The effect of macrophage on phagocytes, there were not found the differences between phagocytic and corrected phagocytic index.

• Applied Biological Chemistry
• /
• 제32권2호
• /
• pp.137-142
• /
• 1989

인삼 에칠아세테이트 추출 분획을 silica gel column chromatography하여 Fr. I, Fr. II 및 Fr. III으로 나눈 다음 항암제 투여로 조혈기능이 억제된 흰쥐에 투여해 본 결과 Fr. III이 감소된 백혈구 및 적혈구 수를 90%까지 회복시켰다. Fr. III에서 두성분을 분리하여 구조를 동정한 결과 ginsenoside $Rh_1$ 및 $Rg_2$로 확인하였다.

• 한국어병학회지
• /
• 제15권1호
• /
• pp.1-7
• /
• 2002

On April of 2000 and February of 2001, a disease characterized by darkening, ascites and enlargement of the spleen occurred in flounder, Paralichthys olivaceus, farmed near northern and eastern coasts of Korea. Various degree of necrosis including liquefactive-type was observed mainly in hemopoietic tissue of spleen and kindney. Certain tissue such as branchial soft tissue including epithelium showed a similar necrotic findings. But some tissues such as intentine had several necrotic clusters of wandering cells, being possibly leukocytes. Necrotic evidences of blood cells were evident in various tissues including hemopoietic tissues. Necrobiotic bodies were frequently encountered in blood smear preparations. Bullet type-virious were detected in the spleenic cells. Each virion was about 500 nm in diameter and 170nm in length. From these results, this disease was thought to be a rhabdoviral disease.

• 대한한의학회지
• /
• 제18권1호
• /
• pp.344-356
• /
• 1997

This experiment was performed in order to study the clinical effect of Sojadodamgangkitang on the pulmonary injury caused by $SO_2$. Healthy adult male rats weighing about 250g were divided into 4 groups-the Normal group, the Control group, the group of Sojadodamgangkitang administration for 5 days after $SO_2$ gas exposure(Sample I), and the group of Sojadodamgangkitang administration for 10 days before and for 5days after $SO_2$ gas exposure(Sample II). The results were obtained as. follows; 1. In comparison with Control group, Sample I and Sample II were revealed significant effect on the total number of leucocyte. 2. In comparison with Control group, Sample I and Sample II were revealed significant effect on. the erythrocyte sedimentation rate. 3. In the effect on the lung weight, Sample I and Sample II were revealed significantly. 4. In the effect on the lung TBA level, only Sample II was revealed significantly. 5. In the effect on the tracheal glycoprotein level, Sample I and Sample II were revealed significantly. According to above results, Sojadodamgangkitang had significant effect on the pulmonary injury caused by $SO_2$ in rats.

• 한국발생생물학회지:발생과생식
• /
• 제14권3호
• /
• pp.207-214
• /
• 2010

The tremendous changes of uterine endometrium are observed during early pregnancy and protease and their inhibitors are involved in regulation of cell proliferation and remodeling of the tissues through remodeling the extracellular matrix (ECM). Some of the proteases and protease inhibitors have been suspected to a factor in endometrial changes but many parts of their expression profiles and the physiological roles are not uncovered. To evaluate the functional roles of them, in this study the expression profiles of proteases and protease inhibitors were analyzed using real-time quantitative PCR analysis. Mmp9 (matrix metalloproteinase 9) mRNA levels peaked on day 4 at the time of implantation. On the other hand, Ela2 (neutrophil elastase, NE) mRNA levels were peaked on day 2 of pregnancy. Its expression were decreased until day 4 of pregnancy but increased rapidly until day 7 of pregnancy and decreased again. NE inhibitor Slpi (secretory leukocyte protease inhibitor, SLPI) mRNA levels were related with the implantation stage and with the levels of Ela2. At the time of implantation the expression levels of Slpi mRNA were about 5 times higher than the Ela2 mRNA in the uterus. In the implantation stage embryos, Mmp9 specific mRNA was only detected at the blastocyst. On the other hand, the expression level of SLPI was higher than that of the Ela2 mRNA at blastocyst and 4.5 day p.c. embryos. Based on these results it is suggested that MMP9, SLPI, and NE have important physiological role in embryo implantation both in uterus and embryos.

• Food Science and Biotechnology
• /
• 제17권2호
• /
• pp.343-348
• /
• 2008

In this study, antioxidant activity of methanol extract of Glycyrrhiza uralensis Fisch (GUE) against $H_2O_2$-induced DNA damage in human leucocytcs and cell death in PC12 cells was determined. The effect of GUE on $H_2O_2$-induced DNA damage in human leucocytcs was evaluated by the comet assay, where GUE ($1-50\;{\mu}g/mL$) was a dose dependent inhibitor of DNA damage induced by $H_2O_2$. The protective effect of GUE against $H_2O_2$-induced damage on PC12 cells was investigated by MTT reduction assay and lactate dehydrogenase release assay. A marked reduction in cell survival induced by $H_2O_2$ was significantly prevented by $1-50\;{\mu}g/mL$ of GUE. The enzyme activity of caspase-3 was elevated in $H_2O_2$-treated PC12 cells, while preincubation with GUE for 30 min inhibited $H_2O_2$-induced caspase-3 activation in a dose-dependent manner. In conclusion, GUE ameliorates $H_2O_2$-induced DNA damage in human leucocytes and has neuroprotective effect by preventing cell death in PC12 cell, suggesting that GU may be a potential candidate for novel therapeutic agents for neuronal diseases associated with oxidative stress.