• Journal of Animal Science and Technology
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• v.46 no.1
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• pp.1-6
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• 2004

This study was conducted to investigate novel genetic variations of MCIR^*3 allele. In general, white spotting or white belt on a black backgroud in pigs is determined by the E$^p$ allele at the MCIR/Extention locus. E$^p$ shares a frameshift mutation with the E$^{D2}$ allele for dominant black color. An oligonucleotide primer set was designed to amplify complete coding sequence of the porcine MCIR gene. The MCIR coding sequences obtained from five breeds those were Landrace(white). Yorkshire(white), Hampshire(belt), Berkshire(spot) and Jeju native black pigs(black), were used for this study. A multiple sequence alignment of the MCIR coding region using Clustal W was performed. The total length of the MCIR coding sequence ranged from 963 to 966 base pairs(bp) among the selected breeds. The sequence analysis of the complete coding region of MCIR was revealed that Hampshire and Jeju native black pig have 3 cytosines deletion and Birkshire has 2 cytosines deletion at codon 23(nt68) in Extention loci. Besides the finding, there were three different missense mutations and a frameshift mutation in the MCIR coding region.

• Journal of Animal Science and Technology
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• v.51 no.3
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• pp.193-200
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• 2009

The pork from black-coated pigs is famous among-consumers for better eating quality. The loci affecting black coat color was identified in pig chromosome 6 in which several genetic effects on pork quality have been reported. The melanocortin 1 receptor (MC1R) gene is a major gene which plays a key role in regulation of eumelanin (black/brown) and phaeomelanin (red/yellow). In this study, the MC1R gene polymorphism was analyzed for pig breed determination and genetic association with pork quality traits. MC1R Ala243Thr variation was analyzed to determine a specific genotype for four commercial pig breeds (Landrace, Yorkshire, Berkshire and, Duroc) and a Korean native pigs (KNP). Then we developed original KNP-specific DNA markers to determine the pork from black-coated pigs using MC1R DNA sequences. The total length of the MC1R coding sequence ranged 1451bp in KNP. KNP had the 0201 allele pertaining to $E^{D1}$ but some of the KNP had the $E^P$ allele, probably reflecting the geneticintrogression of $E^P$ allele into KNP. Furthermore, a relationship between Leu102Pro single nucleotide polymorphism (SNP) genotype and pork quality phenotype were analyzed in F2 reciprocal-crossbred population between KNP and Yorkshire. Association analysis indicated that the allele of the MC1R gene has no effect on pork quality. These results suggest that black coat-color is not directly associated with preferred pork quality, but the black-coat color pig breed may have other genetic components for superior pork quality.

• Journal of Ginseng Research
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• v.35 no.3
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• pp.283-293
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• 2011

With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng (Panax ginseng Meyer), the roots were treated with different dosages of ${\gamma}$-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100 Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides ($Rg_1$, Re, $Rb_1$, $Rb_2$, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4 from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types ($Rg_1$, Re, $Rb_1$, $Rb_2$, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture.

• Journal of Astronomy and Space Sciences
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• v.23 no.4
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• pp.385-396
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• 2006

The International GNSS Service (IGS) has prepared for a transition from the relative phase conte. variation (PCV) to the absolute PCV, because the terrestrial scale problem of the absolute PCV was resolved by estimating the PCV of the GPS satellites. Thus, the GPS data will be processed by using the absolute PCV which will be an IGS standard model in the near future. It is necessary to compare and analyze the results between the relative PCV and the absolute PCV for the establishment of the reliable processing strategy. This research analyzes the effect caused by the absolute PCV via the GPS network data processing. First, the four IGS stations, Daejeon, Suwon, Beijing and Wuhan, are selected to make longer baselines than 1000km, and processed by using the relative PCV and the absolute PCV to examine the effect of the antenna raydome. Beijing and Wuhan stations of which the length of baselines are longer than 1000km show the average difference of 1.33cm in the vertical component, and 2.97cm when the antenna raydomes are considered. Second, the 7 permanent GPS stations among the total 9 stations, operated by Korea Astronomy and Space Science Institute, are processed by applying the relative PCV and the absolute PCV, and their results are compared and analyzed. An insignificant effect of the absolute PCV is shown in Korea regional network with the average difference of 0.12cm in the vertical component.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.2
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• pp.80-85
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• 2002

This study was conducted to investigate the effects of night illumination on the growth and yield of sesame(Sesamum indicum) and perilla(Perilla frutescens). Stem length and height of the first pod in sesame increased, whereas number of branch was reduced and flowering date and maturing date delayed as light intensity of night illumination increased. Number of capsules per plant, ripening ratio and seed yield of sesame were reduced as light intensity of night illumination increased. Yield reduction was greater in Ahnsankkae than in Sodunkkae. Number of nodes increased under night illumination in perilla. Two test cultivars showed reduction in number of branch at 6~10 Lx(22 ㎽ m$^{-2}$ ) intensity of night illumination, even though there was a variation between cultivars. Perilla was sensitive to night illumination far flowering and ripening. Flowering date was delayed by 21 to 28 days at 6~10 Lx intensity of night illumination. Number of flower clusters, number of seeds per flower cluster, and 1,000-seed weight in perilla were significantly reduced and seed yield was reduced by 89 to 98% at 6-10 Lx intensity of night illumination.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.5
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• pp.486-493
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• 2010

This article introduces a novel ultra wideband(UWB) bandpass filter(BPF) with sharp roll-off characteristics in stripline structure. The UWB characteristic is basically obtained from capacitive coupled cross resonator. The resonator has ${\lambda}/2$ length. And at the center of the resonator, two stubs are loaded, one is a ${\lambda}/8$ short-circuited stub and the other is a ${\lambda}/8$ open-circuited stub. The two stubs provide two attenuation poles at lower and upper cutoff frequencies. For input and output lines, two identical capacitively coupled lines have been installed to suppress the unwanted signals in the lower and upper stopbands. The filter has been designed for the U.S. UWB band(3.1~10.6 GHz) with two transmission zeros at 2.4 and 11.1 GHz. The filter has been realized with Low Temperature Core-fired Ceramic(LTCC) green tape which has the dielectric constant of 7.8. Measurement results agree well with HFSS simulation results. Insertion loss less than 0.7 dB and return loss better than 14 dB in the pass band have been measured. The group delay in the center frequency is 0.27 ns and the group delay variation within pass band is less than 0.5 ns. The size of the filter is $6{\times}18{\times}0.6\;mm^3$.

• Journal of Preventive Medicine and Public Health
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• v.31 no.1 s.60
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• pp.1-14
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• 1998

The genetically determined CYP2D6 activity as considered to be associated with cancer susceptibility with inter-individual variation. Genetic polymorphism of CYP2D6(B) and CYP2D6(T) was determined by the two polymerase chain reaction(PCR) and BstN1 and EcoN1 restriction fragment length polymorphisms(RFLP) for 67 lung cancer cases and 95 healthy volunteer controls. The cases were composed of 26 squamous cell carcinoma, 14 small cell carcinoma, 10 adenocarcinoma, 3 large cell undifferentiated carcinoma, and 14 not histologically diagnosed. The results were gained from the 142 subjects (57 cases and 85 controls) who observed successfully in two PCR and BstNl/EcoN1 RELP. Only one and no mutant allele of the CYP2D6(B) and CYP2D6(T) gene was detected, that is, the frequency of mutant allele was very low; 0.7%(1/142) and 0%(0/142), respectively. Detected mutant allele of the CYP2D6(B) was beterozygous type(WM). The odds ratios for lung cancer susceptibility with CYP2D6(B) and CYP2D6(T) genotype were not calculated. These results are similar to the previous understanding that the mutant allele is very rare in Orientals compared to Caucasians, therefore, it considered that CYP2D6(B) and CYP2D6(T) genotypes have maybe no association with lung cancer susceptibility in Koreans. This is the basic data of CYP2D6(B) and CYP2D6(T) genotypes for Koreans. It would be hepful for further study to determine lung cancer susceptibility of Koreans with the data about CYP1A1, CYP2E1, GSTM1 from future study.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.4
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• pp.470-476
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• 1986

In order to find out the optimum seeding date of Job's tears, this study was investigated ecological characters, yield components and yield of Jang Seong native variety at 6 seeding dates (March 20, March 30, April 10, April 20, April 30 and May 10). Emergence period and heading period were shortened as the seeding date was late, and the number of leaves on the main stem was increased as the seeding date was early. Maturing period was 63-66 days when we seeded in March 20, March 30, April 10 and April 20, and it was delayed 68-77 days when we seeded on April 3$^{\circ}$ and May 10. Culm length was longest in April 10 seeding (it was 186.6 cm), and it grew short in the early and the late seeding, but there was no significant difference among seeding dates. Stem diameter was thickest in April 10, and April 20 seeding, that is, thickened to 10.1 mm, and it was thin in the early and the late seeding. Productive tiller number per hill was increased most in April 10 and April 20 seeding, that is, increased to 6.2, and it was decreased in the early and the late seeding. 100 grain weight was heaviest in April 10 seeding; it was 10.7 g, and it was light in the early and the late seeding. Ripeness rate was highest in April 10 seeding (87.4%), and it was lowest in May 10 seeding (76.3%). Grain yield was highest in April 10 seeding (602.8kg per l0a); therefore this period is regarded as optimum seeding date. C. protein, C. fat, C. fiber and C. starch showed significant difference among seeding dates, and C. protein showed possitive correlation (r =0.9811 **) and C. fiber Negative collelation (r =-0.8937*) with seeding dates.

• Journal of Animal Science and Technology
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• v.48 no.6
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• pp.921-932
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• 2006

Management to improve beef tenderness is always been a historical idea, but during the recent past it has become an issue of prime importance to the meat scientists and the industries as well. Variation in tenderness is the prime explanation for consumer’s dissatisfaction for the concern meat. It has been well documented that both postmortem proteolysis and sarcomere length have significant effect on meat tenderness and its consistency. Electrical stimulation and tenderstretch techniques have been used by a number of countries to underpin carcass quality assurance schemes focused on eating quality. The mechanism(s) by which the postmortem interventions improve tenderness (or prevent toughness) has not been fully elucidated. However, it is evident that electrical stimulation accelerates the development of rigor mortis so that prevention of cold shortening is possible and ageing commences at higher temperatures. On the other hand, tendersretch appears to prevent meat toughness via placing tension of the myofibrils and connective matrix during rigor development. Previous findings indicated that electrical stimulation and tenderstretch improved beef tenderness even for fattened cattle under moderate chilling conditions. Recent studies demonstrate beef tenderness to be one of the most important factors determining satisfaction levels of Korean beef consumers. There are number of studies which reported that electrical stimulation and tenderstretch techniques improved Hanwoo tenderness and color. It is believed that the techniques are mostly useful wherein controls of carcass size, fatness and/or chilling regimes are not easy such as Korean beef industry. However, Korean beef industry is one such area where postmortem intervention techniques have not been adopted so far. Taking into consideration of the Korean beef industry, wherein carcass size and fatness varies the post-slaughter intervention technique could be the most feasible measurement to ensure eating quality. The manuscript attempts to highlight the current knowledge aiming primarily towards the assurance of beef tenderness.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.10
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• pp.1404-1410
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• 2008

This study was performed to clarify whether the variation of stress related heat shock protein 70 (HSP70) (GenBank X68213) gene was associated with the nuclear morphological change of in vitro maturation and in vitro capacitation in oocytes of pig ovaries obtained at the slaughterhouse. The nucleic acid substitution of C to G at the 483rd position was found out in HSP70 K1 (290-512) from X68213. The ovaries were categorized into CC, CG, and GG genotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (BsiHKA I). After the second in vitro maturation of immature fresh oocytes, the relation of nuclear morphological change in oocytes with the genotype of HSP70 K1 gene was such that the MII ratios of the genotype GG and CG (46.93% and 42.20%, respectively) were significantly higher than that of the CC genotype (10.71%) (p<0.05). With respect to in vitro maturation of frozen-thawed oocytes by an open pulled straw (OPS) method, the percentage of oocytes matured to MII stage of the CG genotype showed a higher trend than CC and GG genotypes. After the in vitro maturation of immature fresh oocytes and frozen-thawed oocytes by the OPS method, the relation of the pronuclei change in oocytes matured in vitro with HSP70 genotype was assessed, and the result showed that the enlarged sperm heads (ESH) of matured fresh oocytes and frozen-thawed oocytes were 80.0% and 60.0% in the CC genotype, respectively. The CC genotype group had a significantly higher rate of ESH than the CG and the GG genotype group (p<0.05). The ratios of polyspermic invasion were not different among HSP70 of the three genotypes. It was considered that the rate of in vitro maturation of fertilized oocytes was expected to differ according to genotype of the stress related gene.