• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.408-420
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• 2014

Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and $V{\kappa}1$-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than $10^9$ by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ${\sim}10^7$. The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.

• Journal of the Korean Society of Propulsion Engineers
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• v.25 no.4
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• pp.53-58
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• 2021

Based on surrogate model, a hydrocarbon mixture was analyzed by visualizing the impinging break up mechanism in subcritical and supercritical conditions. Decane and methylcyclohexane with different critical pressures and temperatures were selected as experimental fluids. The impinging injector was installed inside the chamber, and the spray was visualized through a speed camera in subcritical and supercritical conditions. The injection condition of the mixture and chamber was kept constant at Pr(P/P_c) = 1, and Tr(T/T_c) was increased from 0.48 to 1.02. As Tr increased, the spray angle increased, and the sheet length decreased as the properties of the mixture reached each critical point. In addition, when the mixture approached the near critical point, it was shown that the change in density gradient was largely observed out of the impinging break up mechanism.

• The Research Journal of the Costume Culture
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• v.31 no.1
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• pp.91-106
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• 2023

This study examines how the concept of gender fluidity-viewing gender identity as a fluid and wide spectrum-is represented in modern knit fashion collections. The period spring/summer 2017-fall/winter 2021, when gender-related fashion keywords started attracting attention, was limited to the last five years, and the results of a case analysis focused on a total of 357 knit fashion photos are as follows. First, the androgynous compromise through the mixing of heterogeneous elements appears as a mix-and-match style due to the patchwork of heterogeneous materials and forms that borrow or share masculinity and femininity. Second, it was confirmed that the dismantling exaggeration caused by the destruction of the size and form of clothes was an avant-garde image that exaggerated the size or length of clothes or destroyed ideas and forms. Third, the exposed sensuality caused by the deformation of the fluid knitting technique was shown in the form of proudly expressing sexuality by exposing the body either using the cut-out technique or through the loose texture of the knit. Knit fashion can highlight decorative effects using handcrafted techniques and express a detailed or coarse sense of organization depending on the density. In addition, since it is possible to create a complex image by juxtaposing and mixing various knit structures, it was confirmed that it is a suitable material for expressing gender fluidity flowing between men and women in fashion.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.634-643
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• 2023

The effects of dietary supplementation with fermented blood meal (FBM) in two forms were evaluated on the growth performance, hematological parameters, innate immune response, antioxidant capacity, digestive enzyme activity and intestinal histology of Japanese eel Anguilla japonica. A commercial powder feed was used as a basal diet (Con). Six other diets were prepared by supplementing 2, 4 and 6% FBM either in powder or liquid form to the Con diet (designated as P2, P4, P6, L2, L4 and L6, respectively). In total 420 eels (initial mean body weight: 55.1±1.71 g) were distributed into 21 tanks (450L) at a density of 20 eels per tank. Three replicate groups of fish were fed one of the seven diets twice daily for 12 weeks. No significant differences were observed in the growth performance and survival among all groups. Fish fed FBM-containing diets showed significantly improved hematological parameters, innate immunity and antioxidant capacity than those in the Con group. Fish fed FBM-containing diets showed significantly enhanced digestive enzyme activity, villus length and goblet cells than those in fish fed Con diet. These results indicate that both powder and liquid FBM could be a good functional feed additive to improve innate immunity and digestion in Japanese eel.

• Proceedings of the KSR Conference
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• 2011.05a
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• pp.680-689
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• 2011

A dynamic analysis procedure is developed to provide a comprehensive estimation of the dynamic response spectrum for locomotive's wheels running over a Pre-Stressed Concrete (PSC) box girder bridge on the Korea high speed railway. The wheel force spectrum with the bridge behavior are analyzed as the dynamic procedure for various running speeds (50~450km/h). The high-speed railway locomotive (KTX) is used as 38-degree of freedom system. Three displacements(vertical, lateral, and longitudinal) and three rotational components (pitching, rolling, and yawing). For one car-body and two bogies as well as five movements except pitching rotation components for four wheel axes forces are considered in the 38-degree of freedom model. Three dimensional frame element is used to model of the PSC box girder bridges, simply supported span length of 40m. The irregulation of rail-way is derived using the exponential spectrum density function under assumption of twelve level tracks conditions based on the normal probability procedure. The dynamic responses of bridge passing through the railway locomotive with high-speed analyzed by Newmark-${\beta}$ method and Runge-Kutta method are compared and contrasted considering the developed models of bridge, track and locomotive comprehensively. The dynamic analyses of wheel forces by Runge-Kutta method which are able to analyze the forces with high frequency running on the bridge and ground rail-way are conducted. Additionally, wheel forces spectrum and three rotational components of vehicle body for three typical running speeds is also presented.

• Journal of The Korean Astronomical Society
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• v.50 no.3
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• pp.61-70
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• 2017

We study the angular correlation function of bright ($K_s{\leq}19.5$) Extremely Red Objects (EROs) selected in the Subaru GTO 2$deg^2$ field. By applying the color selection criteria of $R-K_s$ > 5.0, 5.5, and 6.0, we identify 9055, 4270, and 1777 EROs, respectively. The number density is consistent with similar studies on the optical - NIR color selected red galaxies. The angular correlation functions are derived for EROs with different limiting magnitude and different $R-K_s$ color cut. When we assume that the angular correlation function $w({\theta})$ follows a form of a power-law (i.e., $w({\theta})=A{\theta}^{-{\delta}}$), the value of the amplitude A was larger for brighter EROs compared to the fainter EROs. The result suggests that the brighter, thus more massive high-redshift galaxies, are clustered more strongly compared to the less massive galaxies. Assuming that EROs have redshift distribution centered at ~ 1.1 with ${\sigma}_z=0.15$, the spatial correlation length $r_0$ of the EROs estimated from the observed angular correlation function ranges ${\sim}6-10h^{-1}Mpc$. A comparison with the clustering of dark matter halos in numerical simulation suggests that the EROs are located in most massive dark matter halos and could be progenitors of $L_{\ast}$ elliptical galaxies.

• Geomechanics and Engineering
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• v.7 no.5
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• pp.477-493
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• 2014

This study examines the behaviour of single micropiles subjected to axial tension or compression load in collapsible loess under in-situ moisture content and saturated condition. Five tension loading tests and five compression loading tests on single micropiles were carried out at a typical loess site of the Loess Plateau in Northwest China. A series of laboratory tests, including grain size distribution, specific gravity, moisture content, Atterberg limits, density, granular components, shear strength, and collapse index, were carried out during the micropile loading tests to determine the values of soil parameters. The loess at the test site poses a severe collapse risk upon wetting. The tension or compression load-displacement curves of the micropiles in loess, under in-situ moisture content or saturated condition, can generally be simplified into three distinct regions: an initial linear, a curvilinear transition, and a final linear region, and the bearing capacity or failure load can be interpreted by the L1-L2 method as done in other studies. Micropiles in loess should be considered as frictional pile foundations though the tip resistances are about 10%-15% of the applied loads. Both the tension and compression capacities increase linearly with the ratio of the pile length to the shaft diameter, L/d. For micropiles in loess under in-situ moisture content, the interpreted failure loads or capacities under tension are 66%-87% of those under compression. However, the prewetting of the loess can lead to the reductions of 50% in the tensile bearing capacity and 70% in the compressive bearing capacity.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.43 no.3 s.309
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• pp.31-40
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• 2006

Many researches on path planning and obstacle avoidance for the fundamentals of mobile robot have been done. Informations from various sensors can find obstacles and make path. In spite of many solutions of finding optimal path, each can be applied to only a constrained condition. This means that it is difficult to find a universal algorithm. A optimal path with a complicated computation generates a time delay which cannot avoid moving obstacles. In this paper, we propose the algorithm of path planning and obstacle avoidance for mobile robot. We call the proposed method Random Access Sequence(RAS) method. In the proposed method, a small region is set first and numbers are assigned to its neighbors, then the path is selected using these numbers. It has an advantage of fast planning and simple operation. This means that new path selection may be possible within short time and that helps a robot to avoid obstacle in any direction. When a robot meets moving obstacles, it avoids obstacles in a random direction. RAS method using obstacle information from variable sensors is useful to get minimum path length to goal.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09a
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• pp.813-824
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• 2010

Thermal conductivity of soils is one of the most important parameters to design horizontal ground heat exchangers. It is well known that the thermal conductivity of soil is strongly influenced by its density and water content because of soil's particulate structure. This paper reviewed and evaluated some of the commonly used prediction models for thermal conductivity of soils with the experimental data available in the literature. Semi-theoretical models for two-component materials were found inappropriate to estimate the thermal conductivity of dry state sands. It came out that the model developed by Cote and Konrad gave the best overall prediction for unsaturated sands available in the literature. Also, a parametric analysis is conducted to investigate the effect of thermal conductivity and water content, soil type on the horizontal ground heat exchanger design. The analysis shows that a required pipe length for the horizontal ground heat exchanger is reduced with the increase of soil thermal conductivity and water content. The calculation results also show that the dimension of the horizontal ground heat exchanger can be reduced to a certain extent by using backfilling material with a higher thermal conductivity of solid particles.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.215-216
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• 2013

The silicide is a compound of Si with an electropositive component. Silicides are commonly used in silicon-based microelectronics to reduce resistivity of gate and local interconnect metallization. The popular silicide candidates, CoSi2 and TiSi2, have some limitations. TiSi2 showed line width dependent sheet resistance and has difficulty in transformation of the C49 phase to the low resistive C54. CoSi2 consumes more Si than TiSi2. Nickel silicide is a promising material to substitute for those silicide materials providing several advantages; low resistivity, lower Si consumption and lower formation temperature. Nickel silicide (NiSi) nanowire (NW) has features of a geometrically tiny size in terms of diameter and significantly long directional length, with an excellent electrical conductivity. According to these advantages, NiSi NWs have been applied to various nanoscale applications, such as interconnects [1,2], field emitters [3], and functional microscopy tips [4]. Beside its tiny geometric feature, NW can provide a large surface area at a fixed volume. This makes the material viable for photovoltaic architecture, allowing it to be used to enhance the light-active region [5]. Additionally, a recent report has suggested that an effective antireflection coating-layer can be made with by NiSi NW arrays [6]. A unique growth mechanism of nickel silicide (NiSi) nanowires (NWs) was thermodynamically investigated. The reaction between Ni and Si primarily determines NiSi phases according to the deposition condition. Optimum growth conditions were found at $375^{\circ}C$ leading long and high-density NiSi NWs. The ignition of NiSi NWs is determined by the grain size due to the nucleation limited silicide reaction. A successive Ni diffusion through a silicide layer was traced from a NW grown sample. Otherwise Ni-rich or Si-rich phase induces a film type growth. This work demonstrates specific existence of NiSi NW growth [7].