• 한국초지조사료학회지
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• 제14권1호
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• pp.7-12
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• 1994

This study was undertaken to evaluate the effect of legume reseeding on legume establishment, dry matter yield, quality and botanical composition of grasses in timothy(Ph1eum prarense L.) dominated pasture at alpine area. Establishment number and percentage of legume in timothy dominated pasture were increased with spring, red clover(Tr$olium pratense L.) and gramoxone $treatment Dry matter yield of pasture was increased with fall and grazing after overseeding, but, there waq no difference in legume species. Crude protein content(%) of grasses at the first cutting was increased with red clover and paraquat (gramoxone) treatment. Based on the results, it is suggested that spring in seeding time, red clover in legume species and oveneeding after gramoxone treatment are good for effective renovation of timothy dominated pasture at alpine area.

• 한국초지조사료학회지
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• 제17권3호
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• pp.293-304
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• 1997

A field experiment was conducted at the forage experimental plots, Seoul National University, Suweon h m 1995 to 1996 to determine the effect of legume residues as a N source and N fertilizer on corn (Zea mays L.) silage yield, N uptake, and availability of inorganic N in the soil. Corn was grown following (i) red clover (Trifolium pratense L.), (ii) crimson clover (Trifolium incarnatum L.), (iii) alfalfa (Medicago sativa L.) and (iv) winter fallow. The plots were split into two rates of fertilizer N (0 and 90kg Nlha) in a split-plot experimental plan. Compared with fallow treatment, legumes depleted soil water in the surface 15cm at corn planting by 17 to 26%. As a result, corn emergence was markedly delayed with legume residues by 8 to 11 days. Corn silage DM yield was significantly reduced in the presence of legume residues by 2.0 to 3.4 and 1.5 to 2.5 tonlha compared with winter fallow treatment at 0 and 90kg fertilizer Nlha, respectively, but no significant difference in the corn DM yield was found between legume residue treatments. There was an overall tendency for increased corn yields with 90kg fertilizer Nlha compared to Okg fertilizer Nlha, although not all yield increases were significantly greater. The corn yield response to applied N suggests that a source of N from legume residues was not sufficient for a succeeding corn crop. There was significantly more N (P

• 한국초지조사료학회지
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• 제10권2호
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• pp.110-114
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• 1990

Although corn is regarded as the most feasible forage corp, its relatively low content of protein is the critical we a kness for animal feeding. Many researches have been carried out to improve protein level in corn forage, however, there are no indicatable results but corn-legume intercropping. Plot test and proximate analysis were fullfill to compare dry matter yield and available nutrients of silage corn mono-culture system with those of corn-legume intercropping system of forage plant and silage. The MO culture system were observed by two stage of maturity, milk stage (Aug. 3), yellow stage (Aug. 24), and obtained following results. 1. Dry matter yields per 10 a at milk stage in corn mono-culture system was 596.2kg and corn-legume intercropping systems were 609.0 kg (corn-cowpea), 591.0 kg (corn-soybean) and 563.1 kg (corn-frenchbean), respectively. And comparable to them, 1508.9 kg (corn mono.), 1482.8 kg (corn-cowpea), 1482.6 kg (cornsoybean), 1379.1 kg (corn-frenchbean) were harvested at yellow stage. 2. The general trends of chemical composition by stages of maturity in corn mono-culture systems were higher than that of corn-legume intercropping system. 3. Crude protein content in corn-legume silages were significantly higher than corn mono-culture at yellow stage, except corn-frenchbean intercropping system. 4. Crude fiber content in corn-legume silage was higher than corn mono-culture silage harvested at yellow stage. 5. Consequently, corn-legume intercropping improved nutritional quality of silage than that of corn-monocultivated silage.

• 한국식품과학회지
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• 제20권1호
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• pp.72-78
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• 1988

열대지방에서 재배되는 18종의 콩종자의 구조적인 특성을 분류하기 위하여 그 미세구조를 주로 광학현미경으로 조사하였다. vicieae에 속하는 종실들은 많은 단일전분입자들로 구성된 자엽세포구조를 갖는 starch-rich legume이였으며, phaseoleae중에서는 benas(phaseolus), cow pea, green gram(vigna), hyacinth bean(dolicholus)이 starch-rich legume이였다. 한편 soybean(glycine), winged bean(psophocarpus)는 자엽세포가 대부분 protein body로 구성된 protein-rich legume이고 yam bean (pachyrrhizus)와 cluster bean(cyamopsis)에서는 protein body 보이는 구형물질로 이루어진 자엽세포 구조를 볼 수 있었다. 또한 green gram과 winged bean은 soybean에 비하여 두꺼운 세포벽을 갖고 있었으며 pit-pair가 관찰되었다. Lipid body는 winged bean과 soybean에서 볼 수 있었다. starch-rich legume들은 팥고물 제조과정에서 전분입자들이 파괴되지 않음으로써 특징적인 조직감을 부여하는 red bean이나 benas와 같은 phaseolus의 대체 자원으로 제시될 수 있었다.

• 한국식품과학회지
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• 제49권6호
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• pp.638-643
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• 2017

본 연구에서는 머핀의 품질향상을 위해 두류-밀(1:1) 혼합가루로 제조한 머핀의 품질특성을 살펴보았다. 두류가루를 첨가하였을 때 밀가루만으로 제조한 대조군 머핀에 비해 명도가 감소하고 적색도가 증가하였다. 두류첨가 머핀은 대조군에 비해 상대적으로 많이 존재하는 식이섬유에 의해 높은 수분함량을 나타냈으며 조단백과 조회분의 함량도 대조군에 비해 높게 나타났다. 두류가루가 첨가된 머핀은 대조군에 비해 중량이 높았으나 높이와 부피가 감소하였고 기공의 분포도 다소 불규칙적으로 나타났다. 또한 두류가루 첨가로 인하여 머핀의 경도는 증가하였고 반대로 탄력성과 응집성이 유의적으로 감소하였다. 그러나 관능적으로 두류가루를 첨가한 머핀은 색에 대한 선호도가 대조군에 비해 높았으며 전체적인 기호도는 동부가루와 녹두가루로 제조된 머핀은 대조군에 비해 낮았으나 렌틸가루와 병아리콩가루로 제조한 머핀은 대조군과 유사한 결과를 보였다. 본 연구를 통해 두류가루를 밀가루의 50%까지 대체하여 제조한 머핀의 관능특성은 대조군과 거의 유사했고 영양성은 개선되었으며 이는 두류를 활용한 기능성 제빵 제품 개발의 기초자료로 활용될 수 있을 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.517-522
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• 2007

The aim of this study was to compare in vitro gas production kinetics, metabolizable energy (ME), organic matter digestibility (OMD) and microbial protein (MP) production of widely used legume hays in ruminant nutrition in Turkey. Gas production were determined at 0, 3, 6, 12, 24, 48, 72 and 96 h and their kinetics were described using the equation p = a+b ($1-e^{-ct}$). There were significant differences among legume hays in terms of chemical composition. The crude protein content of legume hays ranged from 11.7 to 18.6% of dry matter (DM); crude fat from 2.1 to 3.5% DM; neutral detergent fiber from 35.6 to 52.0% DM; acid detergent fiber from 32.0 to 35.5% DM and acid detergent lignin 1.7 to 11.0% DM. Total gas production after 96 h incubation ranged between 61.67 and 76.00 ml/0.200 g of substrate. At 24, 72 and 96 h incubation the total gas production for common vetch were significantly (p<0.01) higher than those of the other legume hays. The ME, OMD and MP of legume hays ranged from 9.09 to 11.12 MJ/kg DM, 61.30 to 75.54% and 90.35 to 138.05 g/kg DM, respectively. The ME, OMD and MP of common vetch was significantly (p<0.01) higher than those of the other hays due to low cell-wall contents and high crude protein. At the end of the experiment, differences in chemical composition of legume hays resulted in the differences in the in vitro gas production, gas production kinetics and the estimated parameters such as ME, OMD and MP. Common vetch can be recommended to hay producers and ruminant breeders, due to high ME, OMD and MP production.

• Asian-Australasian Journal of Animal Sciences
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• 제8권2호
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• pp.107-111
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• 1995

An experiment was conducted at Pabna milkshed area under Bathan condition during November, 1992 to February 1993 with 16 milking cows fed legume forage with straw supplementation (treated group) and 8 milking cows fed legume forage only (control group). From the study, the results revealed that supplementation of straw with leguminous diet increases the total dry matter intake (DMI) of 11.83 kg/d/cow for the treated group and 11.53 kg/d/cow for the control group. The average daily legume forage intake was $37.39{\pm}8.67kg/d/cow$ and $49.62{\pm}10.57kg/d/cow$ for the treated and control group respectively and the difference was significant (p < 0.05). The results also exhibited that due to the supplementation of straw, the legume forage intake reduced by 12 kg/d/cow. The forage dry matter intake (DMI) kg/d/cow for the treated and control groups were $6.18{\pm}1.44kg$ and $8.38{\pm}1.95kg$ respectively. The milk production was $8.64{\pm}1.15litre/day$ for the treated group which was significantly (p < 0.05) higher than the control group ($7.74{\pm}1.24litre/day$). The average initial body weight of the supplemented and control groups were $338.06{\pm}39.32kg$ and $329.87{\pm}48.03kg$ respectively. Whereas, the final body weight of supplemented group was $344.33{\pm}35.90kg$ and control group was $330.35{\pm}37.28kg$. It may be concluded that straw supplementation with legumes diet could save legume forage for further use as well as increase milk production.

• 한국초지조사료학회지
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• 제12권3호
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• pp.153-160
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• 1992

본 시험은 고령지 두과목초의 적응성을 구명하기 위하여 표고가 다른 3개지역(600,800,1000m a.s.l)에서 주구를 파종시기(추파.추파), 세구를 혼파조명(알팔파-화본과, 레드크로바-화본과, 화이트크로바-화본과)으로 두고 분할구 배치 3반복으로 수행하였다. 1.600m에서 두과목초의 적응성을 보면 알팔파, 레드클러버 및 화이트클로버 공히 추파를 했을 때 연차간 악화없이 약 30%를 유지하여 적응성이 매우 양호하였다.2. 800m에서는 화치트클로버를 춘파하므로써 연차간에 심한 감소없이 최종 예취시 약 14.0%를 점유하고 있었으며 또한 10a당 건물수량이 가장 많았다. 3. 1000m에서도 800m와 비슷한 경향으로 화이트클로버를 춘파했을 경우 어느정도 적응이 가능하였다. 4. 표고별 두과목초의 비솔 및 수량이 표고가 증가 할수록 현저하게 감소하였으며 알팔파는 800m 이상에서는 생육이 거의 불가능하였다.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.856-863
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• 2013

Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

• The Plant Pathology Journal
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• 제17권2호
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• pp.67-70
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• 2001

Medicago truncatula is a diploid legume plant related to the forage crop alfalfa. Recently, it has been chosen as a model species for genomic studies due to its small genome, self-fertility, short generation time, and high transformation efficiency. M. truncatula engages in symbiosis with nitrogen-fixing soil bacterium Rhizobium meliloti. M. truncatula mutants that are defective in nodulation and developmental processes have been generated. Some of these mutants exhibited altered phenotypes in symbiotic responses such as root hair deformation, expression of nodulin genes, and calcium spiking. Thus, the genes controlling these traits are likely to encode functions that are required for Nod-factor signal transduction pathways. To facilitate genome analysis and map-based cloning of symbiotic genes, a bacterial artificial chromosome library was constructed. An efficient polymerase chain reaction-based screening of the library was devised to fasten physical mapping of specific genomic regions. As a genomics approach, comparative mapping revealed high levels of macro- and microsynteny between M. truncatula and other legume genomes. Expressed sequence tags and microarray profiles reflecting the genetic and biochemical events associated with the development and environmental interactions of M. truncatula are assembled in the databases. Together, these genomics programs will help enrich our understanding of the legume biology.