• Title/Summary/Keyword: leaf symptom

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Characterization of a Novel Cucumber mosaic virus Isolated from Petunia hybrida

  • Han, Kyung-Sook;Choi, Gug-Seoun;Chung, Bong-Nam;Cho, Jeom-Duk;Cho, In-Sook;Kim, Kee-Hong;Kim, Su;Yoon, Ju-Yeon;Choi, Seung-Kook
    • The Plant Pathology Journal
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    • v.28 no.3
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    • pp.299-305
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    • 2012
  • Petunia hybrida is commonly used in landscapes and interiors for its attractive flower. Virus-like foliar symptoms, including a mosaic with dark green islands surrounding the veins and chlorosis on the leaf margins, were observed on a petunia plant from Icheon, Gyeonggido, Korea. Cucumber mosaic virus (CMV) was identified in the symptomatic petunia by serological testing for the presence of CMV coat protein (CP) with a direct antibody-sandwich-enzyme-linked immunosorbent assay. An agent was mechanically transmitted to indicator plant species including Chenopodium quinoa. Examination of the inoculated plant leaves by RT-PCR analysis and electron microscopy revealed the presence of specifically amplified CP products and spherical virions of approximately 28 nm in diameter, respectively, providing confirmation of a CMV infection. Analysis of CP sequences showed that CMV petunia isolate (CMVYJC) shared 82.5-100% amino acid sequence identity with CPs of representative CMV strains. Phylogenetic analysis of CPs supports that CMV-YJC is a member of CMV subgroup IA (CMV-IA) and has biological properties of CMV-IA on host species. To our knowledge, this is the first report of CMV from P. hybrida in Korea.

Two New PVY Strains Isolated from Tobacco Plant in Korea (한국 잎담배에서 분리된 PVY계통)

  • Park E. K.;Kim J. J.;Boo K. S.
    • Korean journal of applied entomology
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    • v.23 no.4 s.61
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    • pp.209-214
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    • 1984
  • Since 1980, burley tobacco plants grown mainly in the western hat of the Korea. have shown two new types of disease symptom. Both symptoms were found to be caused by two different PVY strains : the vein banding type by a PVY strain designated as PVY-VB and necrosis on leaf veins by a PVY strain designated as PVY-VN, Identification of the PVY strains was based on host range test. aphid( Myzus Persicae) transmission test, physical properties, serology, and observation of virus particle morphology. The virus particles were measured to be about 730 nm without any difference in shape or dimensions between the two strains. Both strains also gave a positive reaction to the PVY antiserum in SDS-agar gel double diffusion test. These strains, however, gave a negative reaction to the tobacco etch virus and tobacco vein mottling virus antisera.

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MBCAST: A Forecast Model for Marssonina Blotch of Apple in Korea

  • Kim, Hyo-suk;Jo, Jung-hee;Kang, Wee Soo;Do, Yun Su;Lee, Dong Hyuk;Ahn, Mun-Il;Park, Joo Hyeon;Park, Eun Woo
    • The Plant Pathology Journal
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    • v.35 no.6
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    • pp.585-597
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    • 2019
  • A disease forecast model for Marssonina blotch of apple was developed based on field observations on airborne spore catches, weather conditions, and disease incidence in 2013 and 2015. The model consisted of the airborne spore model (ASM) and the daily infection rate model (IRM). It was found that more than 80% of airborne spore catches for the experiment period was made during the spore liberation period (SLP), which is the period of days of a rain event plus the following 2 days. Of 13 rain-related weather variables, number of rainy days with rainfall ≥ 0.5 mm per day (Lday), maximum hourly rainfall (Pmax) and average daily maximum wind speed (Wavg) during a rain event were most appropriate in describing variations in airborne spore catches during SLP (Si) in 2013. The ASM, Ŝi = 30.280+5.860×Lday×Pmax-2.123×Lday×Pmax×Wavg was statistically significant and capable of predicting the amount of airborne spore catches during SLP in 2015. Assuming that airborne conidia liberated during SLP cause leaf infections resulting in symptom appearance after 21 days of incubation period, there was highly significant correlation between the estimated amount of airborne spore catches (Ŝi) and the daily infection rate (Ri). The IRM, ${\hat{R}}_i$ = 0.039+0.041×Ŝi, was statistically significant but was not able to predict the daily infection rate in 2015. No weather variables showed statistical significance in explaining variations of the daily infection rate in 2013.

Characterization of a Novel Necrotic Response of Glycine max Line 'PI96188' to Xanthomonas axonopodis pv. glycines

  • Han, Sang-Wook;Choi, Min-Seon;Lee, Suk-Ha;Hwang, Duk-Ju;Hwang, Byung-Kook;Heu, Sung-Gi
    • The Plant Pathology Journal
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    • v.23 no.3
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    • pp.193-202
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    • 2007
  • Typical susceptible symptoms of the bacterial pustule disease caused by Xanthomonas axonopodis pv. glycines are pustule and chlorotic haloes that usually occur in leaves of Glycine max plants. The soybean genotype 'PI96188' showed an atypical response to all tested races X. axonopodis pv. glycines, accompanied with necrosis without chlorotic haloes on the underside of the necrotic symptoms. X. axonopodis pv. glycines 8ra grew to levels from 10 to 100 fold lower on PI96188 than on susceptible cultivar Jinjoo1, but 10-fold higher than on the resistant cultivar CNS. The chlorophyll content in PI96188 leaves remained unchanged until 12 days after bacterial infection. Ultrastructural observation showed that the infected leaf cells of PI96188 had intact normal chloroplasts compared to those of the susceptible cultivar Jinjoo1. Chloroplast degradation or the absence of chloroplasts in cells of the infected tissues was observed in Jinjoo1. Senescence-related ACS7 gene was significantly induced in PI96188 compared to those in Jinjoo1 at 2 days after inoculation. While photosynthesis-related rbcS gene showed the dramatic change in Jinjoo1, this gene was constitutively expressed in PI96188. However, expression of the defense-related genes, such as peroxidase and isoflavone synthase in the infected PI96188 leaves was similar to that in Jinjoo1. Together, these results suggest that the novel necrotic symptom in PI96188 is a kind of resistant response different from a typical hypersensitive response in the resistant genotypes.

Effect of Artificial Light Sources on the Growth of Crisphead Lettuce in Plant Factory (식물공장에서 인공광원이 결구상추의 생육에 미치는 영향)

  • 조영렬;한동욱;이용범
    • Journal of Bio-Environment Control
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    • v.7 no.1
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    • pp.35-41
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    • 1998
  • This experiment was conducted to find out the effect of artificial light sources(high-pressure sodium lamp, metal halide lamp and fluorescent lamp) on growth of crisphead lettuce(Lactuca sativa L.) in a plant factory. No significant differences in leaf fresh and dry weight were presented among them. Lettuce plants grown under fluorescent lamp showed the lowest growth. Considering the growth of lettuce and efficiency of lamps, it is worth using HPS(high-pressure sodium) lamp in plant factories. The leaves of lettuce plants grown under artificial light sources showed tipburn symptoms at 14th day after transplanting. The beginning of tipburn symptom have been seen on the ninth to tenth leaves from the cotyledons. It is estimated that the occurrence of tipburn was related to rapid growth and K uptake of the lettuce Plants. The Ca, Mg. Cu and Zn elements tended to be accumulated in the nutrient solutions but the T-N, P, Fe and Mn elements keep in balance in them.

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Environmental Factors Affecting Maturation Rate of Pseudorhecia of Mycosphaerella nawae, the Causal Organism of the Spotted Leaf Castin of Persimmon (감나무 둥근무늬낙엽병균(Mycosphaerella nawae)의 위자낭각 성숙에 영향을 미치는 환경요인)

  • Kwon, Jin-Hyeuk;Kang, Soo-Woong;Park, Chang-Seuk;Kim, Hee-Kyu
    • Korean Journal Plant Pathology
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    • v.13 no.4
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    • pp.215-218
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    • 1997
  • The environmental factors affecting maturity of pseudothecia overwintered in iufected leaves by Mycosphaserella nawae was investigated. The pseudothecia in the overwinteringinfected leaves were matured at the end of April when the average temperature was $14^{\circ}C$ and precipitation was enough. Pseudothecia initiation was preceded by the fungal biomass development in a given host. The maturity of the pseudothecia from the leaves defoliated in early October was earlier and higher than those defoliated in November of the previous year. Pseudothecia development was also positively affected by low temperature. The maturity time, as determined by percentage of matured pseudothecia out of prematured ones, was also 20 days earlier, and the rate of maturity was higher for the leaves overwintered in door, than for those kept in greenhouse. We have providedevidence that the conidia play a significant role in the epidemiology of this pathogen. Many pseudothecia were developed in the leaves infected by artificially inoculated conidia and maturation rate of the pseudothecia was not different from that of the typical symptom by ascospores infection of Mycosphaerella nawae.

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Reaction of Cauliflower Genotypes to Black Rot of Crucifers

  • da Silva, Lincon Rafael;da Silva, Renan Cesar Dias;Cardoso, Atalita Francis;de Mello Pela, Glaucia;Carvalho, Daniel Diego Costa
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.181-185
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    • 2015
  • This study aimed to evaluate six cauliflower genotypes regarding their resistance to black rot and their production performance. To do so, it was conducted two field experiments in Ipameri, $Goi\acute{a}s$, Brazil, in 2012 and 2013. It was used a randomized block design, with four replications (total of 24 plots). Each plot consisted of three planting lines 2.5 m long (six plants/line), spaced 1.0 m apart, for a total area of $7.5m^2$. Evaluations of black rot severity were performed at 45 days after transplanting, this is, 75 days after sowing (DAS), and yield evaluations at 90 to 105 DAS. The Verona 184 genotype was the most resistant to black rot, showing 1.87 and 2.25% of leaf area covered by black rot symptom (LACBRS) in 2012 and 2013. However, it was not among the most productive materials. The yield of the genotypes varied between 15.14 and 25.83 t/ha in both years, Lisvera F1 (21.78 and 24.60 t/ha) and Cindy (19.95 and 23.56 t/ha) being the most productive. However, Lisvera F1 showed 6.37 and 9.37% of LACBRS and Cindy showed 14.25 and 14.87% of LACBRS in 2012 and 2013, being both considered as tolerant to black rot.

Biocontrol of Late Blight (Phytophthora capsici) Disease and Growth Promotion of Pepper by Burkholderia cepacia MPC-7

  • Sopheareth, Mao;Chan, Sarun;Naing, Kyaw Wai;Lee, Yong Seong;Hyun, Hae Nam;Kim, Young Cheol;Kim, Kil Yong
    • The Plant Pathology Journal
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    • v.29 no.1
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    • pp.67-76
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    • 2013
  • A chitinolytic bacterial strain having strong antifungal activity was isolated and identified as Burkholderia cepacia MPC-7 based on 16S rRNA gene analysis. MPC-7 solubilized insoluble phosphorous in hydroxyapatite agar media. It produced gluconic acid and 2-keto-gluconic acid related to the decrease in pH of broth culture. The antagonist produced benzoic acid (BA) and phenylacetic acid (PA). The authentic compounds, BA and PA, showed a broad spectrum of antimicrobial activity against yeast, several bacterial and fungal pathogens in vitro. To demonstrate the biocontrol efficiency of MPC-7 on late blight disease caused by Phyto-phthora capsici, pepper plants in pot trials were treated with modified medium only (M), M plus zoospore inoculation (MP), MPC-7 cultured broth (B) and B plus zoospore inoculation (BP). With the sudden increase in root mortality, plants in MP wilted as early as five days after pathogen inoculation. However, plant in BP did not show any symptom of wilting until five days. Root mortality in BP was markedly reduced for as much as 50%. Plants in B had higher dry weight, P concentration in root, and larger leaf area compared to those in M and MP. These results suggested that B. cepacia MPC-7 should be considered as a candidate for the biological fertilizer as well as antimicrobial agent for pepper plants.

Seed Infection and Damage to Rice Seeds and Seedlings by Seed-Borne Gerlachia oryzae (벼 갈색잎마름병균(Gerlachia oryzae)의 종자감염과 종자감염이 벼종자 및 유묘에 미치는 피해)

  • Kim Wan Gyu;Park Jong Seong;Yu Seung Hun
    • Korean journal of applied entomology
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    • v.23 no.2 s.59
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    • pp.126-131
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    • 1984
  • Twenty one fungi were detected from 26 rice seed samples obtained from Yeongnam Crop Experiment Station, Honam Crop Experiment Station, Chungnam Provincial Office of Rural Development and farmers of Chungnam Province. Gerlachia oryzae was one of frequently detected fungi with $1.0\~45.0\%$ of detection ratio from 22 rice seed samples. The deep-freezing method was selected as the most suitable for routine seed health testing when the blotter method, deep-freezing method and agar plate method were evaluated for efficiency of detecting seed-borne G. oryzae from rice seeds. Our data obtained by seed component plating technique suggested that G. oryzae was present not only on chaff, in endosperm and in seed coat, but also in the embryo. Seed-borne G. oryzae caused seed rot, seedling blight and symptom of brownish discoloration on coleoptile primary and 2nd leaf when the infected seeds were sown in agar of test tube or in soil.

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Improvement of Herbicide Use in Crop Production w. Growth Responses of Soybean (Glycine max) Cultivars to Application Time of Metribuzin Herbicide (제초제의 사용법 개발에 관한 연구 제4보 Metribuzin 사용시간에 따른 대두품종의 생육반응)

  • 구자옥;정순주;이종영
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.2
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    • pp.179-184
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    • 1981
  • The study was conducted to know the differential growth responses of soybean cultivars as affected by various application stages of Metribuzin herbicide, at the University experimental farm of Chonnam Nat'l. Univ, Kwangjoo, 1980. Experimented soybean materials were six cultivars, as Kwanggyo, Dongbuktae, Bongui, Yukwoo 3, Suweon 85, and Suweon 86., . and Laid-out application stages were five as pre-sowing, Pre-emergence, Early Post-emergence, and Late Post-emergence treatment comparing with Control. Results obtained from the study were as follows: Among experimented soybean cultivars, no significantly tolerant cultivar was shown. Highly significant differences in phytotoxicities by Metribuzin treatments were detected among various application stages, and crop tolerances were recognized bigger in order as E. Post-emg. < L. Post-emg. $\ll$ Pre-emg. < Pre-sowing $\leq$ Control. The phytotoxic symptoms of all soybean cuitivars were severely recorded in order of Leaf No. /Plant < Plant height $\ll$ Branch No. /Plant. For the cultivation of most soybean cuitivars, the use of Metribuzin in Pre-sowing or Pre-emergence were recommended, and especially it is expected to follow of more detailed study on soil-incorporation method in Pre-sowing treatment, and rather the tank-mixture system of Metribuzin with others than the mono-application system for the safe-use and enlargement of weeding-spectrum.

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