• 한국연초학회지
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• 제14권2호
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• pp.126-132
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• 1992

This experiment was conducted to examine estimates of heterosis, degree of dominance, heritability and phenotypic and genotypic correlation for several mutant characters in flue-cured tobacco variety. Significant heterosis was noted, recording 2.73 and 6.16% for leaf width, -6.86 and -4.72 for leaf shape index, 19.8 and 23.4% for bacterial wilt disease index in Fl an4 F2 generation, respectively, and -1.44 for days to flower in F2 generation. And also leaf width was appeared partial dominance in Fl, and leaf shape index in Fl and F2, Leaf width in F2 was appeared overdominance. Estimated heritabilities in the broad sense ranged from 0.77 to 0.87 for plant and stalk height and days to flower, while those of leaf number, leaf length and width, leaf shape index and bacterial wilt disease index ranged from 0.50 to 0.65. And the heritabilities in the narrow sense were appeared 0.64 and 0.72 for stalk and plant height, respectively, and the others were ranged from 0.32 to 0.47 Positive genotpic correlations appeared among plant height, stalk height, leaf length and leaf width, and between leaf shape index ands day to flower. And negative genotypic correlations appeared between leaf shape index and plant height and stalk height and leaf width, and between bacterial wilt disease index and leaf length.

• Journal of Plant Biotechnology
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• 제5권3호
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• pp.137-142
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• 2003

The morphology of the leaves and the flowers of angiosperms exhibit remarkable diversity. One of the factors showing the greatest variability of leaf organs is the leaf index, namely, the ratio of leaf length to leaf width. In some cases, different varieties of a single species or closely related species can be distinguished by differences in leaf index. To some extent, the leaf index reflects the morphological adaptation of leaves to a particular environment. In addition, the growth of leaf organs is dependent on the extent of the expansion of leaf cells and on cell proliferation in the cellular level. The rates of the division and enlargement of leaf cells at each stage contribute to the final shape of the leaf, and play important roles throughout leaf development. Thus, the control of leaf shape is related to the control of the shape of cells and the size of cells within the leaf. The shape of flower also reflects the shape of leaf, since floral organs are thought to be a derivative of leaf organs. No good tools have been available for studies of the mechanisms that underlie such biodiversity. However, we have recently obtained some information about molecular mechanisms of leaf morphogenesis as a result of studies of leaves of the model plant, Arabidopsis thaliana. For example, the ANGUSTIFOLIA (AN) gene, a homolog of animal CtBP genes, controls leaf width. AN appears to regulate the polar elongation of leaf cells via control of the arrangement of cortical microtubules. By contrast, the ROTUNDIFOLIA3 (ROT3) gene controls leaf length via the biosynthesis of steroid(s). We provide here an overview of the biodiversity exhibited by the leaf index of angiosperms. Taken together, we can discuss on the possibility of the control of the shapes and size of plant organs by transgenic approaches with the results from basic researches. For example, transgenic plants that overexpressed a wildtype ROT3 gene had longer leaves than parent plants, without any changes in leaf width. Thus, The genes for leaf growth and development, such as ROT3 gene, should be useful tools for the biodesign of plant organs.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.49-55
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• 2003

The morphology of the leaves and the flowers of angiosperms exhibit remarkable diversity. One of the factors showing the greatest variability of leaf organs is the leaf index, namely, the ratio of leaf length to leaf width. In some cases, different varieties of a single species or closely related species can be distinguished by differences in leaf index. To some extent, the leaf index reflects the morphological adaptation of leaves to a particular environment. In addition, the growth of leaf organs is dependent on the extent of the expansion of leaf cells and on cell proliferation in the cellular level. The rates of the division and enlargement of leaf cells at each stage contribute to the final shape of the leaf, and play important roles throughout leaf development. Thus, the control of leaf shape is related to the control of the shape of cells and the size of cells within the leaf. The shape of flower also reflects the shape of leaf, since floral organs are thought to be a derivative of leaf organs. No good tools have been available for studies of the mechanisms that underlie such biodiversity. However, we have recently obtained some information about molecular mechanisms of leaf morphogenesis as a result of studies of leaves of the model plant, Arabidopsis thaliana. For example, the ANGUSTIFOLIA (AN) gene, a homolog of animal CtBP genes, controls leaf width. AN appears to regulate the polar elongation of leaf cells via control of the arrangement of cortical microtubules. By contrast, the ROTUNDIFOLIA3 (ROT3) gene controls leaf length via the biosynthesis of steroid(s). We provide here an overview of the biodiversity exhibited by the leaf index of angiosperms. Taken together, we can discuss on the possibility of the control of the shapes and size of plant organs by transgenic approaches with the results from basic researches. For example, transgenic plants that overexpressed a wild-type ROT3 gene had longer leaves than parent plants, without any changes in leaf width. Thus, The genes for leaf growth and development, such as ROT3 gene, should be useful tools for the biodesign of plant organs.

• Journal of Plant Biotechnology
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• 제38권4호
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• pp.285-292
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• 2011

식물 잎의 발달과정에서 heteroblasty는 외부 환경에 대한 식물의 형태적 적응 방법을 매우 잘 반영하며 이에 따른 변화는 기관의 최종 형태와 크기에 영향을 미친다. Heteroblasty를 나타내는 인자 중에서 leaf index는 단엽식물의 잎의 최종 모양과 크기를 나타내는 대표적인 인자이다. Leaf index는 결국 잎몸에서의 세포 증식과 세포 신장의 두 요인에 의해 결정된다. 비록 세포의 증식과 신장을 조절하는 유전자와 조절 기작들이 연구되고 있으나 큰 청사진을 제시하기에는 아직 미흡하다. 본 연구에서는 발달과정 중 잎의 leaf index 조절에 관여하는 유전자를 밝히고 그 조절 기작을 알아내기 위하여 애기장대 돌연변이체를 이용한 분자유전학적, 생리학적인 실험을 수행하였다. 잎과 잎 세포가 커지는 돌연변이체인 macrophylla (mac)를 선발하여 잎의 확장과정과 leaf index의 이상으로 인해 잎 기관의 모양뿐 만 아니라 heteroblasty에 변화가 발생했다는 사실을 밝혀냈다. 또한 이 돌연변이체는 기존에 알려진 ROTUNDIFOLIA3 (ROT3) 유전자의 점 돌연변이에 의해 일어났다고 판명되었고 mac/rot3-5로 명명되었다. 브라시노스테로이드 처리로 인해 ROT3 유전자의 발현이 음성 되먹임 저해를 받는 것으로 보아 ROT3 유전자가 브라시노스테로이드 생합성에 관여함을 제시하였다. 또한 암상태에서 ROT3 유전자의 발현이 증가하며, mac/rot3-5 돌연변이체가 야생형보다 암반응이 약하게 나타났다. 이러한 분석 결과를 토대로 본 논문은 ROT3 유전자가 잎의 확장과정에서 잎의 leaf index 조절과 고유한 heteroblasty의 정립에 중요한 역할을 수행하며, 브라시노스테로이드 호르몬의 조절을 통하여 음지회피성과 같은 환경조절반응을 수행하고 있다는 새로운 사실을 제시하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.17-17
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• 2022

Leaves are an important organism for photosynthesis and transpiration. The shape of leaf is crucial factor affecting plant architecture. V-shape leaf rolling is enhancing canopy photosynthesis by increasing the CO₂ penetration and the light capture by reducing the shadow between the leaves. Therefore, moderate leaf rolling is thought to more high grain yield per area than flat leaf. We investigated 278 KRICE_CORE accession's Adaxial Leaf Rolling Index (LRI) in first heading using the following equation. For each accession, genomic DNA was used for sequencing. We sequenced the genomics with ~8 X coverage to detect SNPS. Raw reads were aligned against the rice reference (IRGSP 1.0) for SNP identification and genotype calling. To generate genotype data for GWAS, SNPs were filtered with minor allele frequency 0.05. Finally, 841,134 high-quality SNPs were used for our GWAS. The significant threshold was -log10(P)>7.23. From the results, 2 significance SNP were detected. Considering the LD block of 250kbp, 60 candidate gene were selected including Hypothetical gene and Conserved gene. In this poster, we analyzed candidate gene affecting adaxial Leaf Rolling through single-trait GWAS.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.243-243
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• 2022

Leaves are an important organism for photosynthesis and transpiration. The shape of leaf is crucial factor affecting plant architecture. V-shape leaf rolling is enhancing canopy photosynthesis by increasing the CO₂ penetration and the light capture by reducing the shadow between the leaves. Therefore, moderate leaf rolling is thought to more high grain yield per area than flat leaf. We investigated 278 KRICE CORE accession's Adaxial Leaf Rolling Index (LRI) in first heading using the following equation. For each accession, genomic DNA was used for sequencing. We sequenced the genomics with ~8 X coverage to detect SNPS. Raw reads were aligned against the rice reference (IRGSP 1.0) for SNP identification and genotype calling. To generate genotype data for GWAS, SNPs were filtered with minor allele frequency 0.05. Finally, 841,134 high-quality SNPs were used for our GWAS. The significant threshold was -log10(P) >7.23. From the results, 2 significance SNP were detected. Considering the LD block of 250kbp, 60 candidate gene were selected including Hypothetical gene and Conserved gene. In this poster, we analyzed candidate gene affecting adaxial Leaf Rolling through single-trait GWAS.

• 한국연초학회지
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• 제15권1호
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• pp.34-48
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• 1993

This experiment were conducted to investigate heterosis and combining ability for several mutant characters by analyzing dialled crosses of flue-cured tobacco. In a dialled cross of 3 flue-cured varieties and the mutant line 83H -5, the heterosis was somewhat higher in Fl than in F2. For growth character, the heterosis was 0.28-6.03% in plant height, leaf number, leaf shape index and yield, and was 43.2% for bacterial wilt disease index. The mutant line 83H-5 showed significantly negative GCA effect for plant height, leaf width and bacterial wilt disease index in Fl and F2, leaf length in F2, and positive GCA effect for total alkaloids, total nitrogen in Fl and days to flower in F2, respectively. Specific combining ability(SCA) in 83H-5 x Hicks was significant in negative effect for leaf length(F2), number of leaves(F2), leaf shape(F1, F2), bacterial wilt(F2) and alkaloids(F1), and in 83H-5 x NC 2326 in positive effect for leaf length(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in positive effect for plant height(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in Positive effect for Plant height(F1, F2), leaf length(F2) and yield(F1, F2).

• 한국환경과학회지
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• 제33권6호
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• pp.383-402
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• 2024

Variations in lettuce growth and quality were observed depending on the type of artificial light source. The RGB LED treatment resulted in thick leaf development, leading to higher fresh weight, dry weight, and relative growth rates. Two cultivars, "Tomalin" and "seonpunggold," exhibited increased anthocyanin content and dark red leaf color under conditions of RGB LED treatment. Additionally, they exhibited high chlorophyll content under conditions of RGB LED and RGBFR LED treatments. Particularly, under Red LED treatment, the plants showed elongated leaves with narrow widths, resulting in a higher leaf shape index and a tendency towards leaf curling. Therefore, RGB LED lighting which appropriately blends red, blue, and green lights, is more effective than single lighr sources at improving lettuce growth and quality.

• 한국연초학회지
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• 제22권1호
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• pp.25-30
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• 2000

Bacterial wilt(Ralstonia solanacearum) is one of the major diseases of flue-cured tobacco (Nicotiana tabacum L.) in the world. This study was conducted to investigate degree of dominance, selection, and correlation between leaf shape and degree of bacterial wilt resistance in flue-cured tobacco. The degree of disease caused by bacterial wilt was evaluated in parents, F$_1$, F$_2$ and F$_3$ populations of two crosses, BY 4 x NC 95 and BY 4 x Coker 86, in the infected field. The leaf shape index was also measured in parents and F$_2$ population of BY 4 x NC 95. The incidence of bacterial wilt was observed in the middle of June and peaked in late July, when the highest value of pathogen density reached 1.0 x 10$^{6}$ colony forming unit per gram. It was concluded that the inheritance mode of risestance to bacterial wilt in the above two crosses of susceptible and resistant varieties was recessive and polygenic. The resistance to bacterial wilt was significantly correlated with leaf shape in F2 generation of BY 4 x NC 95. But certain plants having narrower leaves were also resistant to bacterial wilt. It is considered that the bacterial wilt resistant lines having narrower leaves could be selected. The selection for bacterial wilt resistance in the F$_2$ population might be effective.

• 한국환경생태학회지
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• 제35권6호
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• pp.644-658
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• 2021

도시 생태계에서 우점 수종의 형질 특성은 분획화 및 고립된 식생, 그리고 생물상호작용 감소 등 환경 변화에 대한 종의 형질 적응과 생태계 기능에 대한 통찰력을 제공한다. 우리는 한국의 서울 중심에서 남쪽 방향의 4개 산지에서 환경 요인 (지리, 기상 및 토양 속성)을 포함하여 모두 40개체 팥배나무 (Sorbus alnifolia)에서 측정한 4개 유형의 잎 형질 (Leaf area, Specific leaf area, Leaf dry mass content, Leaf shape index)과 7개 유형의 번식 형질 (Fruit width, Fruit length, Fruit shape, Fruit dry weight, Fruit dry matter content, Seed weight 및 Seed ratio)의 평균과 분산 양상, 그리고 형질 조합에 대한 다변량 분석을 실시하였다. 측정된 환경 요인은 조사 장소의 개별성을 나타내었지만, 도심 식생이 더 건조하고 생장도일이 길었다. 도심에서 팥배나무의 잎은 작고 무거워지며, 열매는 길쭉하고 가벼운 종자를 생산하여, 오랜 도시화 영향에 따른 형질 반응들이 관찰되었다. 우리의 연구는 식생 파편화 및 고립화에 따른 생물 상호작용 감소를 나타내는 도심 환경에서 팥배나무 집단의 성장 및 번식 기제의 변화를 확인하였다. 우리는 축소된 생물 상호작용 규모, 그리고 대기 오염 같은 부정적 환경 요인이 많은 도시에서 핵심 수종의 기능과 지속성에 관해 제한적이지만 뚜렷한 생태 정보를 제공한다.