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http://dx.doi.org/10.5010/JPB.2011.38.4.285

MACROPHYLLA/ROTUNDIFOLIA3 gene of Arabidopsis controls leaf index during leaf development  

Jun, Sang-Eun (Department of Molecular Biotechnology, Dong-A University)
Chandrasekhar, Thummala (Department of Molecular Biotechnology, Dong-A University)
Cho, Kiu-Hyung (Department of Molecular Biotechnology, Dong-A University)
Yi, Young-Byung (Department of Molecular Biotechnology, Dong-A University)
Hyung, Nam-In (Department of Plant Science and Technology, Sangmyung University)
Nam, Jae-Sung (Department of Molecular Biotechnology, Dong-A University)
Kim, Gyung-Tae (Department of Molecular Biotechnology, Dong-A University)
Publication Information
Journal of Plant Biotechnology / v.38, no.4, 2011 , pp. 285-292 More about this Journal
Abstract
In plants, heteroblasty reflects the morphological adaptation during leaf development according to the external environmental condition and affects the final shape and size of organ. Among parameters displaying heteroblasty, leaf index is an important and typical one to represent the shape and size of simple leaves. Leaf index factor is eventually determined by cell proliferation and cell expansion in leaf blades. Although several regulators and their mechanisms controlling the cell division and cell expansion in leaf development have been studied, it does not fully provide a blueprint of organ formation and morphogenesis during environmental changes. To investigate genes and their mechanisms controlling leaf index during leaf development, we carried out molecular-genetic and physiological experiments using an Arabidopsis mutant. In this study, we identified macrophylla (mac) which had enlarged leaves. In detail, the mac mutant showed alteration in leaf index and cell expansion in direction of width and length, resulting in not only modification of leaf shape but also disruption of heteroblasty. Molecular-genetic studies indicated that mac mutant had point mutation in ROTUDIFOLIA3 (ROT3) gene involved in brassinosteroid biosynthesis and was an allele of rot3-1 mutant. We named it mac/rot3-5 mutant. The expression of ROT3 gene was controlled by negative feedback inhibition by the treatment of brassinosteroid hormone, suggesting that ROT3 gene was involved in brassinosteroid biosynthesis. In dark condition, in addition, the expression of ROT3 gene was up-regulated and mac/rot3-5 mutant showed lower response, compare to wild type in petiole elongation. This study suggests that ROT3 gene has an important role in control of leaf index during leaf expansion process for proper environmental adaptation, such as shade avoidance syndrome, via the control of brassinosteroid biosynthesis.
Keywords
Brassinosteroid; Heteroblasty; Leaf index; macrophylla; Shade avoidance;
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1 Hunter C, Willmann MR, Wu G, Yoshikawa M, de la Luz Gutierrez-Nava M, Poethig RS (2006) Trans-acting siRNA-mediated repression of ETTIN and ARF4 regulates heteroblasty in Arabidopsis. Development 133:2975-2981
2 Phillips AL, Ward DA, Uknes S, Appleford NE, Lange T, Huttly AK, Gaskin P, Graebe JE, Hedden P (1995) Isolation and expression of three gibberellin 20-oxidase cDNA clones from Arabidopsis. Plant Physiol 108:1049-1057   DOI
3 Steeves TA, Sussex IM (1989) Patterns in plant development. (2nd eds), Cambridge University Press, New York, pp 147-175
4 Sunderland N (1960) Cell division and expansion in the growth of the leaf. J. Exp. Bot. 11:68-80   DOI
5 Szekeres M, Németh K, Koncz-Kálmán Z, Mathur J, Kauschmann A, Altmann T, Rédei GP, Nagy F, Schell I, Koncz C (1996) Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell 85:171-182   DOI
6 Tanaka K, Asami T, Yoshida S, Nakamura Y, Matsuo T, Okamoto S (2005) Brassinosteroid homeostasis in Arabidopsis is ensured by feedback expressions of multiple genes involved in its metabolism. Plant Physiol 138:1117-1125   DOI
7 Tsuge T, Tsukaya H, Uchimiya H (1996) Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh. Development 122:1589-1600
8 Tsukaya H (2002) The leaf index: heteroblasty, natural variation, and the genetic control of polar processes of leaf expansion. Plant Cell Physiol 43:372-378   DOI
9 Tsukaya H, Kozuka T, Kim GT (2002) Genetic control of petiole length in Arabidopsis thaliana. Plant Cell Physiol 43:1221-1228   DOI
10 Tsukaya H, Shoda K, Kim GT, Uchimiya H (2000) Heteroblasty in Arabidopsis thaliana (L.) Heynh. Planta 210:536-542   DOI
11 Kim GT, Fujioka S, Kozuka T, Tax FE, Takatsuto S, Yoshida S, Tsukaya H (2005a) CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana. Plant J 41:710-721   DOI
12 Kozuka, T, Horiguchi G, Kim GT, Ohgishi M, Sakai T, Tsukaya H (2005) The different growth response of the Arabidopsis thaliana leaf blade and the petiole during shade avoidance are regulated by photoreceptors and sugar. Plant Cell Physiol 46:213-223   DOI
13 Kim GT, Tsukaya H, Saito Y, Uchimiya H (1999). Changes in the shapes of leaves and flowers upon overexpression of the novel cytochrome P450 in Arabidopsis. Proc Natl Acad. Sci. USA 96:9433-9437   DOI
14 Kim GT, Tsukaya H, Uchimiya H (1998) The ROTUNDIFOLIA3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P-450 family that is required for the regulated polar elongation of leaf cells. Genes Dev. 12:2381-2391   DOI
15 Kim GT, Yano S, Kozuka T, Tsukaya H (2005b) Photomorphogenesis of leaves: shade-avoidance and differentiation of sun and shade leaves. Photochem Photobiol Sci 4:770-774   DOI
16 Kozuka T, Kobayashi J, Horiguchi G, Dnura T, Sakakibara H, Tsukaya H, Nagatani A (2010) Involvement of auxin and brassinosteroid in the regulation of petiole elongation under the shade. Plant Physiol 153:1608-1618   DOI
17 Maksymowych R (1963) Cell division and cell elongation in leaf development of Xanthium pensylvanicum. Amer J Bot 50:891-901   DOI
18 Murashige T, Skoog G (1963) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473-497   DOI
19 Ohnishi T, Szatmari AM, Watanabe B, Fujita S, Bancos S, Koncz C, Lafos M, Shibata K, Yokota T, Sakata K, Szekeres M, Mizutani M (2009) C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis. Plant Cell 18:3275-3288   DOI
20 Arkebuer TJ, Norman JM (1995) From cell growth to leaf growth: I. Coupling cell division and cell expansion. Agron. J 87:99-105   DOI
21 Ashby E (1948) Studies in the morphogenesis of leaves. I. An essay on leaf shape. New Phytol 47:153-176   DOI
22 Avery GS JR (1933) Structure and development of the tobacco leaf. Amer. J Bot 20:565-592   DOI
23 Bancos ST, Nomura T, Sato G, Molnar G, Bishop J, Koncz C, Yokota T, Nagy F, Szekeres M (2002) Regulation of transcript levels of the Arabidopsis cytochrome p450 genes involved in brassinosteroid biosynthesis. Plant Physiol 130:504-513   DOI
24 Cho KH, Jun SE, Jeong SJ, Yi YB, Kim GT (2007). Regulation of cell size and cell number by LANCEOLATA1 gene in Arabidopsis. Kor J Life Science 17:1-5   과학기술학회마을   DOI
25 Cunninghame ME, Hyndon RF (1986) The relationship between the distribution of periclinal cell divisions in the shoot apex and leaf initiation. Ann Bot 57:737-746   DOI