• 제목/요약/키워드: leaf disease

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피노믹스 시스템을 위한 식물 잎의 질병 검출 및 분류 (Detection and Classification of Leaf Diseases for Phenomics System)

  • 박관익;심규동;견민수;이상화;백정현;박종일
    • 방송공학회논문지
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    • 제27권6호
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    • pp.923-935
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    • 2022
  • 본 논문에서는 스마트팜 시스템에서 재배 중인 식물 잎의 질병을 검출하고, 질병 유형을 분류하는 방법을 제안한다. 영상으로부터식물 잎의 컬러 정보와 질병 유형의 형태 정보를 다층 퍼셉트론(MLP) 모델을 이용하여 학습한다. 1단계에서는 입력된 영상의 컬러분포를 분석하여 질병 존재 여부를 판단한다. 1단계의 질병 존재 가능성이 높은 영상에 대하여 2단계에서는 Mean shift clustering을 이용하여 작은 영역으로 분할하고, 각 분할된 영역 단위로 컬러 정보를 추출하여 제안한 Color Network에 의하여 질병 여부를 판별한다. 컬러 분할된 영역이 Color Network에 의하여 질병으로 판별되면, 3단계에서는 그 영역의 형태 정보를 추출하여 제안한 Shape Network를 이용하여 질병의 유형을 분류한다. 사과나무 잎과 서양 양상추(Iceberg)에서 발생하는 두 가지 대분류 유형의 질병에 대하여, 제안한 기법은 작은 영역 단위로는 92.3%의 잎 질병 검출률을 보였으며, 보통 2개 이상의 질병 영역이 존재하는 한 장의 영상 단위로는 99.3% 이상의 검출률을 보였다. 본 논문에서 제안한 방법은 스마트팜 환경에서 잎 식물의 질병 여부를 조기에 발견할 수 있으며, 대상 식물에 따른 추가 학습 없이 다양한 식물과 질병 유형으로 확대 적용이 가능하다.

Pseudomonas cichorii와 P.viridiflava에 의한 Ficus 속 식물의 세균성뿌리썩음병 및 세균성잎마름병 (Bacterial Root Rot and Bacterial Leaf Blght of Ficus spp. by Pseudomonas cichorii and P. viridiiflava in Korea)

  • 이은정;차재경;최재을
    • 식물병연구
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    • 제6권1호
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    • pp.6-9
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    • 2000
  • 1998년 수원, 성남, 양재, 대전의 비닐하우스에서 재배되고 있는 인삼고무나무(Ficus retusa), 황금대만고무나무(Ficus retusa \`Golden leaf\`), 벤자민고무나무(Ficus benjamina)에 새로운 세균병이 관찰되었다. 인삼고무나무의 뿌리에 무름증상을 보이는 세균병을 \"P.cichorii\"에 의한 인삼고무나무의 세균성뿌리썩음병\", 황금대만고무나무와 벤자민고무나무에 마름증상을 보이는 세균병을 \"P.viridiflava에 의한 황금대만고무나무와 벤자민고무나무의 세균성잎마름병\"으로 명명할 것을 제안한다.마름병\"으로 명명할 것을 제안한다.제안한다.

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Neopestalotiopsis Leaf Blight, an Emerging Concern on Leatherleaf Fern in Indonesia

  • Ani Widiastuti;Indah Khofifah Aruan;Alvina Clara Giovanni;Barokati Tsaniyah;Tri Joko;Achmadi Priyatmojo
    • 식물병연구
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    • 제30권1호
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    • pp.82-87
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    • 2024
  • Leatherleaf fern (Rumohra adiantiformis) is an important ornamental plant in Indonesia and global. Green fern leaves with bold dark green color with long shelf-life, attract florists as decoration. Indonesia is one important leatherleaf fern exporters, however currently an outbreak of leaf blight decreased production significantly. Initial symptom was reddish brown spots from edge of leaf, which was gradually followed by dark-brown necrotic lesions causing leaf blight and dried. This is a study to do Koch-Postulate approach and molecular identification, to identify the pathogen of the "new emerging disease" reported. Based on multigene analysis using primers from ITS, β-tub and tef1-α gene markers, the pathogen was identified as Neopestalotiopsis sp. All sequences have been deposited in GenBank with accession number of OR905551 (ITS), OR899817 (ßtubulin) and OR899816 (TEF). This Neopestalotiopsis leaf blight causes an emerging concern in leatherleaf fern in Indonesia and global biosecurity because it infected an export commodity.

Differential Responses of Soybean Cultivars to Cercospora sojina Isolates, the Causal Agent of Frogeye Leaf Spot in Korea

  • Kim, Ji-Seong;Lee, Young-Su;Kim, Sung-Kee;Kim, Ki-Deok;Kim, Jin-Won
    • The Plant Pathology Journal
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    • 제27권2호
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    • pp.183-186
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    • 2011
  • During the summer of 2005, specimens of soybean cultivars (Daepung, Daewon, Hwanggeum and Taegwang) showing frogeye leaf spot (FLS) were obtained from various areas in Korea. Eight isolates identified as Cercospora sojina were inoculated on the adaxial leaf surfaces of 63 Korean soybean cultivars; the disease responses to each isolate were evaluated 14 days and 21 days after inoculation. Based on the disease responses (resistant or susceptible) of the cultivars by the isolates, a set of cultivars (Anpeong, Bogwang, Cheongdu No. 1, Cheongja No. 3, Dachae, Daemang, Jangwon, Namhae, Sowon, Taegwang) were selected and inoculated with seven isolates for further testing pathogenic variance. Interestingly, 6 out of 7 tested C. sojina isolates revealed differential ability in infecting different soybean cultivars. This result may indicate the possibility of new race occurrence or pathogenic variation; this also presents evidence for prevalent FLS occurrence during humid and hot weather in Korea.

재배양식에 따른 더덕 병해 발생양상 (Incidence of Diseases in Codonopsis lanceolata with Different Cultivation Method)

  • 김주희;최정식
    • 한국식물병리학회지
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    • 제14권6호
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    • pp.676-681
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    • 1998
  • Disease incidence of Codonopsis lanceolata was surveyed at the major cultivating fields in Chonbuk province in 1996 to 1997. The main diseases of Codonopsis lanceolata were ovserved as leaf spot caused by Septoria codonopsis, anthracnose by Glomerella cingulata, brown leaf spot by Cercospora sp., rust by Coleosporium koreanum, powdery mildew by Erysiphe sp., Fusarium wilt caused by Fusarium oxyporum, and white root rot by Sclerotium rolfsii. Anthracnose, leaf spot and brown leaf spot occurred severely on leaves from early July to late August. They were caused early fallen leaves. Fusarium wilt and white root rot occurred severely on stem and below the soil line in late August. They resulted in withering to death or chlorosis and fallen of leaves. Disease incidence of Codonopsis lanceolata was also substantially different in occurrence with a method of cultivation in late growth stage. Fusarium wilt and white root rot were more severe with a method of no support cultivation than those with a method of support cultivation with a stick. Fusarium wilt occurred 48.8% in a method of no support cultivation but 3.1% in a method of support cultivation with a stick. And white root rot occurred 18.9% in a method of no support cultivation but 0.3% in a method of no support cultivation with a stick. Thus, it proved that soil-borne diseases could be controlled support cultivation with a stick.

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황색종 연초에 있어서 변이체의 조합능력 및 Heterosis (Combining-Ability and Heterosis for Mutant Character of Quantitative Characters in Flue-Cured Tobacco Varieties(Nicotiana tabacum L))

  • 정석훈;이승철;김흥배
    • 한국연초학회지
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    • 제15권1호
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    • pp.34-48
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    • 1993
  • This experiment were conducted to investigate heterosis and combining ability for several mutant characters by analyzing dialled crosses of flue-cured tobacco. In a dialled cross of 3 flue-cured varieties and the mutant line 83H -5, the heterosis was somewhat higher in Fl than in F2. For growth character, the heterosis was 0.28-6.03% in plant height, leaf number, leaf shape index and yield, and was 43.2% for bacterial wilt disease index. The mutant line 83H-5 showed significantly negative GCA effect for plant height, leaf width and bacterial wilt disease index in Fl and F2, leaf length in F2, and positive GCA effect for total alkaloids, total nitrogen in Fl and days to flower in F2, respectively. Specific combining ability(SCA) in 83H-5 x Hicks was significant in negative effect for leaf length(F2), number of leaves(F2), leaf shape(F1, F2), bacterial wilt(F2) and alkaloids(F1), and in 83H-5 x NC 2326 in positive effect for leaf length(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in positive effect for plant height(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in Positive effect for Plant height(F1, F2), leaf length(F2) and yield(F1, F2).

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접종방법에 따른 딸기모무늬병원균의 발병도 검증 및 친환경 방제 효과 (Disease Severity of Angular Leaf Spot Disease by Different Inoculation Method and Eco-Friendly Control Efficacy in Strawberry.)

  • 김다란;강근혜;조현지;윤혜숙;명인식;곽연식
    • 농약과학회지
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    • 제20권1호
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    • pp.35-40
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    • 2016
  • 딸기 세균성모무늬병은 딸기를 기주로 하여 발생하며 큰 피해를 발생시킨다. 병원균의 전반은 관수의 형태, 감염된 모주의 이용 그리고 작업자에 의해 이루어지며 현재까지 명확한 화학적방제법이 개발되어 있지 않다. 2010년, 국내의 모무늬병원균에 대한 최초 보고에 따르면 병징으로서 잎 뒷면에 수침상의 각진 병반이 형성되며 습도가 높고 적합한 환경에서 세균침출액을 형성하게 되고 또한 진전된 경우 잎에서 적갈색의 병반이 형성된다고 보고하였다. 현재까지 이루어진 연구에 따르면 딸기 세균성모무늬병에 대하여 두 가지 품종이 저항성을 지니고 있다는 연구 결과가 있으며 감염된 포장에서는 수확량의 10%를 감소를 나타낸다고 알려져 있다. 그러나 이와 같은 모무늬병원균에 대한 국내에서의 활발한 연구가 이루어져 있지 않은 상황이다. 이에 본 연구는 딸기 모무늬병에 대하여 원인병원균이 Xanthomonas fragariae 병원균에 의하여 발생됨을 보고하는 바이며 병원성검증실험 진행한 결과 국내의 딸기재배 품종인 매향과 설향 중 매향이 감수성을 나타냄을 확인하였다. 또한 NaDCC처리시 98%이상의 방제가가 확인되었으므로 친환경방제제로서의 가능성을 제시하며 이러한 연구결과들은 국내에 발생되어있는 딸기 세균성모무늬병에 대하여 기초적 자료로서 보고하는 바이다.

Characterization of the host reaction of some citrus plants with Xanthomonas axonopodis pv. citri, causing citrus bacterial canker disease.

  • Myung, Inn-Shik;Hyun, Jae-Wook;Kim, Kwang-Sik;Lee, Sung-Chan;Lim, Han-Chul
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.120.3-121
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    • 2003
  • Relative degree of resistance of citrus to Xanthomonas axonopodis pv. citri, the causal bacterium of canker, was investigated. Growth rate of a bacterium in leaf tissues after infiltration, disease incidence, and percent of lesion area were compared. By using growth rate[(GR=(At - A$\sub$t-1/)/A$\sub$t-1] host plants were differentiated into susceptible and resistant. Growth rates reached to peak at 40 hrs after inoculation and then declined. The growth rate in leaf tissues of a moderately susceptible cultivar, Citrus sinensis vu. Lane late(sweet orange), was the highest, and those of C. unshiu ${\times}$ C. sinensis(kiyomi), C. junos(yuzu), [(Citrus. unshiu x C. sinensis) x C. reticulata] (shiranuhi), and C. unshiu(satuma mandarin) were similar. This result indicates that the growth rate of the bacterium in leaf tissues can be effectively used for evaluation of disease resistance for citrus plants to X. axonopodis pv. citri. The disease on sweet orange occurred earlier than relatively resistant citrus plants tested. The percent of lesion area on leaf was also higher in sweet orange than those of satsuma mandarin, shiranuhi and kiyomi, and yuzu. The disease severity was highest on sweet orange and followed by kiyomi, shiranuhi, satsuma mandarin, and yuzu.

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시설하우스에서 Corynespora cassiicola에 의해 발생하는 오이 갈색무늬병 (A Target Leaf Spot Disease Caused by Corynespora cassiicola on Cucumber Cultivated in Green House)

  • 권미경;양광열;조백호
    • 식물병연구
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    • 제10권2호
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    • pp.121-125
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    • 2004
  • 2000년도와 2001년도에 남부지방(구례, 별교, 영암)의 시설 내 오이재배지에서 갈색무늬병에 피해가 심하였다. 잎에서 전형적인 수침상 병반이 생기고 초기에 황색 halo를 지닌 갈색반점이 생긴 후 점차 불규칙하고 큰 병반으로 진전되어 때때로 낙엽을 유발하였다. 감염잎으로부터 분리한 병원균은 분생자경에 연쇄상 혹은 단일 분생포자를 형성하였고 분생포자는 거의 무색에서 올리브 갈색 내지는 갈색을 띠었으며 모양은 7-11개의 위격벽을 지닌 원통형,역곤봉형, 직립형, 만곡형 등이었다. 또한 분리 병원균의 ITS영역의 염기서열은 Corynespora cassciicola와 일치하였다. 형태적, ITS영역 분석을 기초로 분리된 병원균은 C. cassiicola로 동정하였다. 병원균의 생육은 3$0^{\circ}C$ Czapek Solution Agar 배지에서 가장 좋았으며, 발병환경 조사 결과 3$0^{\circ}C$에서 18시간 이상 습전시간이 유지될 때 발병이 높아, 고온과 식물의 긴 습전기간이 발병량의 증가를 가져오는 요인이었다. 또한 국내 품종들을 대상으로 오이갈색무늬병에 대한 감수성과 저항성 품종들을 선발하였다.

Leaf Rot and Leaf Ring Spot Caused by Rhizoctonia solani in Chinese Cabbage

  • Shim, Chang-Ki;Kim, Min-Jeong;Kim, Yong-Ki;Jee, Hyeong-Jin;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Yun, Jong-Chul
    • 식물병연구
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    • 제19권4호
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    • pp.300-307
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    • 2013
  • This study was conducted to determine the occurrence of leaf rot and leaf ring spot, caused by Rhizoctonia solani in Chinese cabbage under seedling nursery and cultivation greenhouses. Symptoms of leaf rot and leaf ring spot were found in three Chinese cabbage cultivars, Brassica campestris subsp. pekinensis, 'Ryeokgwang', 'Daetong', and 'CR mat'. In Hwacheon, the disease incidence was 73.8% in the seedling stage of the Chinese cabbage. In Icheon, the symptoms were observed on the upper leaves of the Chinese cabbage cultivar, 'Norangmini' with 20.5% of disease incidence. The symptoms appeared as primary lesions consisting of small, circular necrotic ring spots with gray color, 1.4-3.0 mm in diameter, accompanied by secondary rot lesions with large irregular borders of leaves. The color of mycelial mat of 20 isolates was dark brown and light brown. The average hyphal diameter of all the isolates was within 5.01-11.12 ${\mu}m$. Among the 20 strains isolated from Chinese cabbage, 16 isolates and four isolates anastomosed with the AG-1 (IB) and AG-1 (IC), respectively. Twenty isolates tested were only virulent on foliage parts of Chinese cabbage leaves but were avirulent on stem parts of the plants. Based on the mycological characteristics and pathogenicity test on host plants, the fungus was identified as Rhizoctonia solani.