• Title/Summary/Keyword: leaf development

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Determination of Regression Model for Estimating Root Fresh Weight Using Maximum Leaf Length and Width of Root Vegetables Grown in Reclaimed Land (간척지 재배 근채류의 최대 엽장과 엽폭을 이용한 지하부 생체중 추정용 회귀 모델 결정)

  • Jung, Dae Ho;Yi, Pyoung Ho;Lee, In-Bog
    • Korean Journal of Environmental Agriculture
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    • v.39 no.3
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    • pp.204-213
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    • 2020
  • BACKGROUND: Since the number of crops cultivated in reclaimed land is huge, it is very difficult to quantify the total crop production. Therefore, a non-destructive method for predicting crop production is needed. Salt tolerant root vegetables such as red beets and sugar beet are suitable for cultivation in reclaimed land. If their underground biomass can be predicted, it helps to estimate crop productivity. Objectives of this study are to investigate maximum leaf length and weight of red beet, sugar beet, and turnips grown in reclaimed land, and to determine optimal model with regression analysis for linear and allometric growth models. METHODS AND RESULTS: Maximum leaf length, width, and root fresh weight of red beets, sugar beets, and turnips were measured. Ten linear models and six allometric growth models were selected for estimation of root fresh weight and non-linear regression analysis was conducted. The allometric growth model, which have a variable multiplied by square of maximum leaf length and maximum leaf width, showed highest R2 values of 0.67, 0.70, and 0.49 for red beets, sugar beets, and turnips, respectively. Validation results of the models for red beets and sugar beets showed the R2 values of 0.63 and 0.65, respectively. However, the model for turnips showed the R2 value of 0.48. The allometric growth model was suitable for estimating the root fresh weight of red beets and sugar beets, but the accuracy for turnips was relatively low. CONCLUSION: The regression models established in this study may be useful to estimate the total production of root vegetables cultivated in reclaimed land, and it will be used as a non-destructive method for prediction of crop information.

Ultra-rapid Real-time PCR for the Detection of Tomato yellow leaf curl virus (초고속 Real-time PCR을 이용한 Tomato yellow leaf curl virus의 신속진단)

  • Kim, Tack-Soo;Choi, Seung-Kook;Ko, Min-Jung;Lee, Minho;Choi, Hyung Seok;Lee, Se-Weon;Park, Kyungseok;Park, Jin-Woo
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.298-303
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    • 2012
  • Tomato yellow leaf curl virus (TYLCV), transmitted exclusively by the whitefly (Bemisia tabaci) in a circulative manner is one of the most important virus in tomato. Since the first report of TYLCV incidence in Korea in 2008, the virus has rapidly spread nationwide. TYLCV currently causes serious economic losses in tomato production in Korea. Early detection of TYLCV is one of the most important methods to allow rouging of infected tomato plants to minimize the spread of TYLCV disease. We have developed an ultra-rapid and sensitive real-time polymerase chain reaction (PCR) using a new designed real-time PCR system, GenSpectorTM TMC-1000 that is a small and portable real-time PCR machine requiring only a $5{\mu}l$ reaction volume on microchips. The new system provides ultra-high speed reaction (30 cycles in less than 15 minutes) and melting curve analysis for amplified TYLCV products. These results suggest that the short reaction time and ultra sensitivity of the GenSpector$^{TM}$-based real-time PCR technique is suitable for monitoring epidemics and pre-pandemic TYLCV disease. This is the first report for plant virus detection using an ultra-rapid real-time PCR system.

Development of Resistance Evaluation Method for Powdery Mildew (Leveillula taurica) in Capsicum spp. (고추 흰가루병 저항성 평가방법 개발)

  • Kim, Su;Kim, Dong-Hwi;Kim, Dae-Hyun;Han, Kyung-Sook;Han, You-Kyoung;Lee, Seong-Chan;Cho, Myeong-Cheoul;Yang, Eun-Young;Kim, Kee-Hong
    • Research in Plant Disease
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    • v.17 no.3
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    • pp.311-316
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    • 2011
  • Pepper powdery mildew causes increasingly economical damage due to increased cultivation of pepper in greenhouses. To assess resistance of pepper resources against pepper powdery mildew, we developed a novel evaluation formula for pepper resistance against powdery mildew. The evaluation formula named S index is as follows; (number of the highest disease leaf/top leaf number)-(number of the lowest disease leaf/top leaf number). Positive correlation (81%, P = 0.01) between S index and authentic disease leaf rate was observed from the pepper plants infected by powdery mildew. Various pepper species from our genetic resources were evaluated to identify pepper varieties conferring resistance against powdery mild using S-index. Capsicum frutescens accessions 3CA131 and C. baccatum accessions 3CA0162, 3CA174, 3CA176 showed high resistance to powdery mildew, but none of C. annuum was resistant. Results suggest that S-index proposed in this study is useful to assess resistance evaluation of powdery mildew in chili pepper breeding.

Study on functional improvement of peanut sprouts by LEDs

  • Shin, So-Hee;Choi, So-Ra;Song, Eun-Ju;Song, Young-Eun;Choi, Min-Kyung;Han, Hyun-Ah;Lee, Ki-Kwon;Lee, In-Sok;Chung, Nam-Jin
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.277-277
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    • 2017
  • The research was carried out to investigate a total polyphenol content, antioxidant activity, amino acid and resveratrol content of peanut sprouts (cotyledon, epicotyl, leaf, hypocotyl, root), in different light (white, blue, red, F-red, UV-A, UV-B, UV-C) conditions for 24 hours. Peanut seeds were sown on a $27.5{\times}15.9{\times}13cm$ tray and grown at the $25^{\circ}C$ under the dark condition for 14 days. Total polyphenolic contents of epicotyl and leaf were about 288mg GAE/100g in blue light. The DPPH radical scavenging of cotyledon and hypocotyl were 1.3~1.5 times (63%) and 2 times (40%) compared to control (43%, 19%), respectively. As to ABTS activity, its activity was increased by all LEDs treatment, Especially, the highest ABTS activity of the hypocotyl and leaf was shown as 99.1% in blue light. The essential amino acid content of hypocotyl and leaf was increased 1.9 times in the UV-B, 1.6 times in red, and 1.5 times in F-red, respectively. The non-essential amino acid content was increased by all LEDs treatment in hypocotyl and leaf. The content of resveratrol was increased by 1.3 times in UV-B compared to that of other tissues. Assessing inclusively, this study showed that there was a significantly positive effect between increase of physiological substance activity and LED light treatment, resulting in stably producing peanut sprouts. Therefore, a material treated with LEDs is thought to be useful as a functional food resources.

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Studies on the Pear Abnormal Leaf Spot Disease 1. Occurrence and Damage (배나무잎 이상반점증상에 관한 연구 1. 발생상황과 피해)

  • 남기웅;김충회
    • Korean Journal Plant Pathology
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    • v.10 no.3
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    • pp.169-174
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    • 1994
  • A new unidentified pear leaf spot disease presumed to first occur in the late 1970's has recently become prevalent over the pear growing areas, and caused the greatest problem for pear production in Korea. The disease began to develop on pear leaves at mid- to late May, peaked at mid- to late une, but stopped further development until September in cool climate. Leaf lesions are 0.9∼2.5 mm in diam., oval or irregular to rectangular in shape, first appeared reddish purple, later changed to dark brown, and to whitish grey in the late season. Lesions were limited to appear only on the mature, hardened leaves, initially from leaf margin or near the leaf veins, and later scattered over the leaf surface. Individual lesions usually did not enlarge, but often coalesced each other, commonly causing shot holes and eventual early falling. The disease was most severe on the major pear cultivars Niitaka and Okusankichi ranged with 4 to 100% infections in trees, depending on the orchards, but not on the cultivar Chojuro. Damages from the disease included lower fruit weight, and higher acid and less sugar content in fruits, resulting in lowering the overall fruit quality. Etiology of the disease including identification of the causal organism is in a separate paper.

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Studies on the Nutritional Physiology of Soybean 6. Variatio of Potassium at the Various Position of Leaf on the Main Stem (대두의 영양생리학적 연구 6. 엽위별 가리의 변이)

  • 이순희
    • Journal of Plant Biology
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    • v.17 no.3
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    • pp.127-136
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    • 1974
  • The effect of potassium metabolism on the soybean leaves was studied with comparison of other elements during the successive growing period. The results were as follows; 1. The percentage of potassium content showed remarkable increase not only in the first compound leaf at a stage which was growing vigorously and producing new leaves, but also in the fifth compound leaf at a stage which was taking a active metabolism of nitrogen and carbohydrate but not producing new leaves. However, the percentage of potassium content was decreased in the second compound leaf than in the first one. Such a result could be regarded as a potassium removal from mature leaves into immature and flowing out from stoma through respiration. During the pod-development the percentage of potassium content in the soybean leaf was decreased. 2. If nitrogen, phosphorus and potassium were added excessively in the nutrient solution, the percentage of potassium content in the soybean leaf had increased. The effects of these elements showed a remakable increase in the excessive plot of nitrogen than in that of phosphorus. At early stage the redtarded effect of phosphorus on the growth of soybean could be covered by potassium, however, at late stage it could not. The growth of soybean plant was much more inhibited by potassium, compared with nitrogen and phosphorus. New leaves could not be produced in the potassium deficient soybean plant after the third compound leaf. The normal growth of soybean plant could not be observed if only one element was excessively added to the culture solution, compared with the deficiency of other two elements.

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A Simple and Reliable Molecular Detection Method for Tomato yellow leaf curl virus in Solanum lycopersicum without DNA Extraction

  • Yoon, Ju-Yeon;Kim, Su;Choi, Gug-Seoun;Choi, Seung-Kook
    • Research in Plant Disease
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    • v.21 no.3
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    • pp.180-185
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    • 2015
  • In the present work, a pair of primers specific to Tomato yellow leaf curl virus (TYLCV) was designed to allow specific amplification of DNA fragments from any TYLCV isolates using an extensive alignment of the complete genome sequences of TYLCV isolates deposited in the GenBank database. A pair of primers which allows the specific amplification of tomato ${\beta}$-tubulin gene was also analyzed as an internal PCR control. A duplex PCR method with the developed primer sets showed that TYLCV could be directly detected from the leaf crude sap of infected tomato plants. In addition, our developed duplex PCR method could determine PCR errors for TYLCV diagnosis, suggesting that this duplex PCR method with the primer sets is a good tool for specific and sensitive TYLCV diagnosis. The developed duplex PCR method was further verified from tomato samples collected from some farms in Korea, suggesting that this developed PCR method is a simple and reliable tool for rapid and large-scale TYLCV detections in tomato plants.

Food plants suitable for mass rearing of the coconut hispine beetle Brontispa longissima

  • Yamashita, Ai;Winotai, Amporn;Nakamura, Satoshi;Takasu, Keiji
    • Korean Journal of Agricultural Science
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    • v.36 no.1
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    • pp.57-61
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    • 2009
  • The invasive pest Brontispa longissima(Coleoptera: Chrysomelidae), native to Indonesia and Papua New Guinea, has extended its distribution to Australia, Asia and Pacific islands and caused serious leaf damages of the coconut palm Cocos nuciferain the invaded regions. Although biological control using parasitic wasps has successfully reduced population density and leaf damage levels, this pest and its natural enemies have not been efficiently producedin conventional methods using young leaves of C. nucifera. In the present study, we examined suitability of plants easily available in Thailand and Japan for mass rearing of this pest to develop effective mass rearing system of this pest. Mature, green leaves of the palms were also suitable for immature development and adult reproduction of this pest. Since mature leaves of C. nucifera are more abundant and less contaminated with fungus than the unopened leaf buds, mature leaves could be a promising plant diet for mass rearing of B. longissima. Ornamental palms such as Hyophorbe lagenicaulis and Washingtonia filifera were also suitable for immature development and reproduction of B. longissima. Away from palms, the cattail Typha spp. can sustain immature development and adult reproduction of B. longissima. In the area where C. nucifera is rare or not available, W. filifera or Typha spp. would be good food plants for mass rearing of this pest.

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Analysis on the Growth Environment of Disporum smilacinum A. GRAY for Development of Shade-tolerance Groundcover Plant (내음성 지표식물 개발을 위한 애기나리의 생육환경분석)

  • 이기철;박슬기
    • Journal of the Korean Institute of Landscape Architecture
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    • v.19 no.2
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    • pp.65-74
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    • 1991
  • The purpose of this study was to find the basic application of Disporum genus in landscape-arc-hitectual space. Environment of native site (Daeducksan, Piseulsan) and growth characteristics of experiment plot were investigated and analyzed in this study. The results of this study were as follows; 1. In the case of environment of native site, the relative light intensity was about 10% and distribution of species were primarily north slope. Gradient of Daeducksan and Piseulsan were 10$^{\circ}$, 18$^{\circ}$ Soil textures was Lic both and field capacity of Daeducksan and Piseulsan were 69%, 73.5%. The soil pH of Daeducksan and Piseulsan were 6.1 and 5.8. The both content of organic matter and C.E.C. were high markedly. The P2O5 content of Daeducksan showed high but total nitrogen showed nearly the same level when comparing with the common dry field condition. 2. In tree layer, Daeducksan composed of broadleaved forest of 80% involving Quercus variabilis, and Piseulsan composed of coniferous forest of 80% involving Pinus densiflora on the other hand. Daeducksan, therefore, was more appearance species than Piseulsan in herbaceous layer. 3. Result in ANOVA Test, significance followed with light intensity was recognized in plant height, leaf width, leaf length, leaf thick and No. of leaf, while in the case of soil was not. Plant height was longest in 1% and leaf width, leaf length was largest in 25%. Therefore ornamental value of leaf was the very best in 25%. 4. Result in correlation coefficient analysis, plant height was correlated leaf width, leaf length. The longer plant height, the smaller leaf width, leaf length. In relative light intensity of 1%, flower was hardly expected because of the more shade, the less flower. Disporum smilacinum is expected great use as ground covers in the shades of tall-building or hdavy woods.

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Development of Digital Leaf Authoring Tool for Virtual Landscape Production (가상 조경 생성을위한 디지털 잎 저작도구 개발)

  • Kim, Jinmo
    • Journal of the Korea Computer Graphics Society
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    • v.21 no.5
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    • pp.1-10
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    • 2015
  • This study proposes a method of developing authoring tool that can easily and intuitively generate diverse digital leaves that compose virtual landscape. The main system of the proposed authoring tool consists of deformation method for the contour of leaf blade based on image warping, procedural modeling of leaf vein and visualization method based on mathematical model that expresses the color and brightness of leaves. First, the proposed authoring tool receives leaf input image and searches for contour information on the leaf blades. It then designs leaf blade deformation method that can generate diverse shapes of leaf blades in an intuitive structure using feature-based image warping. Based on the computed leaf blade contour, the system implements the generalized procedural modeling method suitable for the authoring tool that generates natural vein patterns appropriate for the leaf blade shape. Finally, the system applies visualization function that can express color and brightness of leaves and their changes over time using a mathematical model based on convolution sums of divisor functions. This paper provides texture support function so that the digital leaves that were generated using the proposed authoring tool can be used in a variety of three-dimensional digital contents field.