• Title/Summary/Keyword: leaf area index

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Single-trait GWAS of Leaf Rolling Index with the Korean Rice Germplasm

  • ByeongYong Jeong;Muhyun Kim;Tae-Ho Ham;Seong-Gyu Jang;Ah-Rim Lee;Min young Song;Soon-Wook Kwon;Joohyun Lee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.17-17
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    • 2022
  • Leaves are an important organism for photosynthesis and transpiration. The shape of leaf is crucial factor affecting plant architecture. V-shape leaf rolling is enhancing canopy photosynthesis by increasing the CO2 penetration and the light capture by reducing the shadow between the leaves. Therefore, moderate leaf rolling is thought to more high grain yield per area than flat leaf. We investigated 278 KRICE_CORE accession's Adaxial Leaf Rolling Index (LRI) in first heading using the following equation. For each accession, genomic DNA was used for sequencing. We sequenced the genomics with ~8 X coverage to detect SNPS. Raw reads were aligned against the rice reference (IRGSP 1.0) for SNP identification and genotype calling. To generate genotype data for GWAS, SNPs were filtered with minor allele frequency 0.05. Finally, 841,134 high-quality SNPs were used for our GWAS. The significant threshold was -log10(P)>7.23. From the results, 2 significance SNP were detected. Considering the LD block of 250kbp, 60 candidate gene were selected including Hypothetical gene and Conserved gene. In this poster, we analyzed candidate gene affecting adaxial Leaf Rolling through single-trait GWAS.

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Single-trait GWAS of Leaf Rolling Index with the Korean Rice Germplasm

  • ByeongYong Jeong;Muhyun Kim;Tae-Ho Ham;Seong-Gyu Jang;Ah-Rim Lee;Min young Song;Soon-Wook Kwon;Joohyun Lee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.243-243
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    • 2022
  • Leaves are an important organism for photosynthesis and transpiration. The shape of leaf is crucial factor affecting plant architecture. V-shape leaf rolling is enhancing canopy photosynthesis by increasing the CO2 penetration and the light capture by reducing the shadow between the leaves. Therefore, moderate leaf rolling is thought to more high grain yield per area than flat leaf. We investigated 278 KRICE CORE accession's Adaxial Leaf Rolling Index (LRI) in first heading using the following equation. For each accession, genomic DNA was used for sequencing. We sequenced the genomics with ~8 X coverage to detect SNPS. Raw reads were aligned against the rice reference (IRGSP 1.0) for SNP identification and genotype calling. To generate genotype data for GWAS, SNPs were filtered with minor allele frequency 0.05. Finally, 841,134 high-quality SNPs were used for our GWAS. The significant threshold was -log10(P) >7.23. From the results, 2 significance SNP were detected. Considering the LD block of 250kbp, 60 candidate gene were selected including Hypothetical gene and Conserved gene. In this poster, we analyzed candidate gene affecting adaxial Leaf Rolling through single-trait GWAS.

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Relation Between the Growth Habitat and the Leaf Quality of Cultivated Mulberry Plants (뽕나무의 생육특성과 엽질과의 관계)

  • 김준호
    • Journal of Plant Biology
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    • v.17 no.3
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    • pp.118-126
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    • 1974
  • Mulberry plants cultivated in mid-Korea were studied on the growth habitat, canopy type, productive structure and vertical light intensity in relation to the quality of mulberry leaves for sericulture. The growth in length of new branch of summer cut mulberry in spring was vigorous on the uppermost of old shoot. Numbers of leaves on a branch were barely 4 leaves on lower than 20th branch from base, but those were 13 leaves on higher than 24th branch. Area of leaves completely expanded was broad on high branch, but narrow on low one. The form of canopy, the type of productive structure and the vertical light distribution depend upon varieties of cultivated mulberry plants were classified according to characteristic of each variety in two groups, respectively; globular and columnar form in canopy, broad leaves and narrow leaves types in productive structure, and seriously obstructured the light and fairly transmitted the light into lower leaf stratum invertical light distribution. Out of varieties of mulberry investigated, Suwon No. 4 and Rosang were belong to the former characters, but Illchiroe and Kaeryangsubun were to the latters. The values of leaf dry matter index and dry matter production produced by leaves were increased in direct proportion to the height of leaf stratum; the leaf dry matter index of the uppermost leaf was twice as much that of the lowest, and the dry matter production by the highest leaf was 17 times compared with that by the lowest leaf. It is assumed that the meagre leaves occurred in the lower leaf stratum of Suwon No. 4 and Rosang were caused to diminished the dry matter index and dry matter production in the under leaves of plant, which were affected by receiving the weak light.

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The Application of Satellite Data to Land Surface Process Parameterization in ARPS Model (ARPS 모형 지면 과정 모수화에 위성 자료의 응용)

  • Ha, Kyung-Ja;Suh, Ae-Sook;Chung, Hyo-Sang
    • Journal of the Korean Association of Geographic Information Studies
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    • v.1 no.1
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    • pp.99-108
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    • 1998
  • In order to represent the surface characteristics in local meteorological model, soil type, vegetation index, surface roughness length, surface albedo and leaf area index should be prescribed on the surface process parameterization. In this study, the $1^{\circ}/1^{\circ}leaf$ area index, surface roughness length, and snow free surface albedo and fine mesh NDVI with seasonal variation derived from the satellite observation were applied to the land surface process parameterization. From comparison between with and without satellite data in the interactions between biosphere and atmosphere, land and atmosphere, the sensitivity of the simulated heat, energy and water vapor fluxes, ground temperature, wind, canopy water content, specific humidity, and precipitation fields were investigated.

Estimation of Specific Leaf Area Index Using Direct Method by Leaf Litter in Gwangneung, Mt. Taewha and Mt. Gariwang (광릉숲, 태화산, 가리왕산 활엽수림에서 낙엽에 의한 수종별 엽면적지수 추정)

  • Kwon, Boram;Jeon, Jihyeon;Kim, Hyun Seok;Yi, Myong Jong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.1
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    • pp.1-15
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    • 2016
  • Annual litterfall production and leaf area index (LAI, $m^2/m^2$) were estimated using litter traps in Gwangneung, Mt. Taewha and Mt. Gariwang. Annual total litter fall production including branch, bark, others was the highest in Gwangneung($7497.3{\pm}326.5kg/ha/yr$), which had the highest basal area at late successional stage, and followed by Mt. Taewha($5929.1{\pm}225.8kg/ha/yr$) and Mt. Gariwang($3,210.1{\pm}220.1kg/ha/yr$). Mt. Gariwang had the lowest litterfall production due to high elevation and short growing season even with the higher stand density and basal area than Mt. Taewha. Similarly, LAI, which was calculated by multiplying the mass of leaf litter with specific leaf area, was the highest in Gwangneung($5.99{\pm}0.69$) and followed by Mt. Taewha($5.20{\pm}0.24$) and Mt. Gariwang($4.06{\pm}0.42$) and the upper canopy species had the highest leaf area index in every sites (Gwangneung : 4.72, Mt. Taewha : 3.08, Mt. Gariwang : 2.19). However, species specific LAI estimation based on the relationship between basal area and leaf area was limited due to upper canopy species non-proportionality of basal area with LAI. In addition, the comparison between direct and indirect LAI measurement showed the importance of canopy clumping, especially at high density. Our study emphasized the necessity of direct LAI measurement using litter fall traps especially at temperate deciduous forest with diverse species.

Changes in Leaf and Reproductive Traits of Mountain Ash (Sorbus alnifolia) as Urban Flourisher in the Seoul Metropolitan, South Korea (한국 서울 식생의 번성자로서 팥배나무의 형질 변화 양상)

  • Jung, Song-Hie;Cho, Yong-Chan;Lee, Chang-Seok
    • Korean Journal of Environment and Ecology
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    • v.35 no.6
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    • pp.644-658
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    • 2021
  • Understanding the functional traits of dominant species in urban ecosystems provides insight into species' trait adaptation and ecosystem function in response to fragmented and isolated urban vegetation and reduced biological interactions. This study compared means and variances of environmental factors (geographic, meteorological, and soil attributes) and 4 leaf traits (leaf area, specific leaf area, leaf dry mass content, and leaf shape index) and 7 reproductive traits (fruit width, fruit length, fruit shape, fruit dry weight, fruit dry matter content, seed weight, and seed ratio) measured of 40 Sorbus alnifolia individuals in four mountainous areas south of Seoul downtown, South Korea. We then performed the multivariate analysis of trait combinations. While the measured environmental factors indicated the individuality of the survey sites, the urban vegetation was drier and had a longer growth period. The leaves of S. alnifolia in the urban areas were smaller and heavier, and the fruits produced longer and lighter seeds, showing the traits affected by long urbanization. The study confirmed changes in the growth and reproduction mechanism of the S. alnifolia population under the urban environment, indicating reduced biological interaction due to vegetation fragmentation and isolation. This study provides limited but distinct ecological information about the function and persistence of key species in cities with a reduced scale of biological interactions and many negative environmental factors such as air pollution.

Evaluation of Growth Diagnosis in Rice Field using Spectral Characteristics, LAI, and SPAD (분광반사특성과 엽면적지수 및 SPAD를 이용한 벼의 성장단계별 생육상태의 평가)

  • Park, Jong-Hwa;Shin, Hyoung-Sub;Park, Jin-Ki
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.805-809
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    • 2008
  • Measurement of leaf area index (LAI) is useful for understanding rice growth, water use, and canopy light interception. The top nitrogen content(TNC) per unit area is an important quantitative index of the condition of nitrogen nutrition in rice production. The rapid and simple method of estimation of TNC, with the use of the existing nondestructive analyzing instruments chlorophyll meter SPAD-502 and plant canopy analyzer (PCA) LAI-2000, was scrutinized. Destructive measurement is time consuming and labor intensive. Our objective was to evaluate sampling procedures using the Li-Cor LI-1800, LAI 2000 plant canopy analyzer (PCA) for nondestructive estimation of rice LAI, and SPAD-502 on the Northern Plains of Cheongju. The LAI estimated by PCA tended to underestimate the LAI determined by actual measurement by about 20%. The estimation of LAI by PCA was judged to have a sufficient accuracy as a practical technique. A high positive correlation was obtained between the values of the SPAD reading and LAI. NDVI and LAI also showed a very high correlation. The values of the SPAD reading and LAI, and NDVI gave a high positive correlation. These results indicated that the method described in this study was effective as a simple and rapid method for the estimation of rice growth.

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Effect of N-levels on Growth and Dry Matter Accumlation of Sorghum-Sudangrass Hybrid ( Sorghum bicolor $\times$ S. sudanense ) , Pioneer 988 (질소시비수준이 Pioneer 988 ( Sorghum bicolor $\times$ S.Sudanesse ) 의 엽생육 및 건물생산에 미치는 영향)

  • 김상덕;윤익석
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.4 no.2
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    • pp.158-163
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    • 1983
  • The purpose of this experiment was to find out fundamental data for the cultivation of sorghum-sudangrass hybrid. Leaf area index and dry matter accumulation were investigated with 3-levels of nitrogen application,15, 25, 35kg per 10a during the period of initial growth from the 8th leaf emergence to the late blooming stage. The results obtained were as follows: Leaf emergence increased as growth progressed and in the same pattern at all N-levels. After the heading stage, the value was 15 at all N-levels. Leaf area index (LAI) increased as growth progressed and rapidly till the booting stage. At 15kg N-level the values were larger till the ear formation stage, and at 35tg N-level the values were larger than other N-levels after the booting stage. At the late blooming stage LAI reached 8.13 at 35kg N-level. The value of dry matter accumulation was the highest at 35kg N-level at the late blooming stage, and as was the same in the value of crop growth rate (CGR). There was a tendency that before the booting stage the enlargement of leaf area contributed to dry matter accumulation and after the booting stage did the increase of net assimilation rate (NAR) .

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Selection of the Most Sensitive Waveband Reflectance for Normalized Difference Vegetation Index Calculation to Predict Rice Crop Growth and Grain Yield

  • Nguyen Hung The;Lee Byun Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.5
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    • pp.394-406
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    • 2004
  • A split-plot designed experiment including four rice varieties and 10 nitrogen levels was conducted in 2003 at the Experimental Farm of Seoul National University, Suwon, Korea. Before heading, hyperspectral canopy reflectance (300-1100nm with 1.55nm step) and nine crop variables such as shoot fresh weight (SFW), leaf area index, leaf dry weight, shoot dry weight, leaf N concentration, shoot N concentration, leaf N density, shoot N density and N nutrition index were measured at 54 and 72 days after transplanting. Grain yield, total number of spikelets, number of filled spikelets and 1000-grain weight were measured at harvest. 14,635 narrow-band NDVIs as combinations of reflectances at wavelength ${\lambda}l\;and\;{\lambda}2$ were correlated to the nine crop variables. One NDVI with the highest correlation coefficient with a given crop variable was selected as the NDVI of the best fit for this crop variable. As expected, models to predict crop variables before heading using the NDVI of the best fit had higher $r^2$ (>10\%)$ than those using common broad- band NDVI red or NDVI green. The models with the narrow-band NDVI of the best fit overcame broad- band NDVI saturation at high LAI values as frequently reported. Models using NDVIs of the best fit at booting showed higher predictive capacity for yield and yield component than models using crop variables.

Characteristics of Leaf Morphology and Genetic Variation of the Rare Woody Plant, Berchemia racemosa var. magna (희귀수종 먹넌출 엽의 형태적 특성과 유전변이)

  • Song, Jeong-Ho;Lim, Hyo-In;Jang, Kyung-Hwan;Han, Jingyu
    • Korean Journal of Plant Resources
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    • v.26 no.5
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    • pp.613-618
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    • 2013
  • This study was conducted to investigate the morphological characteristics of leaf and the genetic diversity of Berchemia racemosa var. magna which is only found in Anmyeon Island of South Korea. ANOVA test showed that there were significant differences among individuals within population in all 10 leaf characteristics. Average characteristics of 39 individuals were 11.8 cm in leaf length, 7.1 cm in leaf width, 1.67 in leaf index, 5.4 cm in upper 1/3 width, 6.2 cm in lower 1/3 width, 3.6 cm in petiole length, 0.19 mm in leaf thickness, 11.5 ea. in number of veins (left), 11.4 ea. in number of veins (right) and 61.7 $cm^2$ in leaf area, respectively. Except for leaf thickness (18.8%), petiole length (21.7%) and leaf area (22.0%), the coefficients of variation of most leaf characteristics were relatively low (<15.0%). A total of 50 bands was generated from 8 selected I-SSR primers. The estimates of genetic variation were 1.719 in effective number of alleles ($A_e$), 26.0% in proportion of polymorphic bands (P), 0.410 in expected heterozygosity ($H_e$) and 0.598 in Shannon's diversity index (S.I.), respectively. In spite of the small number and the limited distribution, the B. racemosa var. magna population in Anmyeon Island showed high genetic diversity.