• Asian-Australasian Journal of Animal Sciences
• /
• 제29권12호
• /
• pp.1696-1701
• /
• 2016

Japanese quail is still used as a model for poultry research because of their usefulness as laying, meat, and laboratory animals. Microsatellite markers are the most widely used molecular markers, due to their relative ease of scoring and high levels of polymorphism. The objective of the research was to determine genetic diversity and population genetic structures of selected Japanese quail lines (high body weight 1 [HBW1], HBW2, low body weight [LBW], and layer [L]) throughout 15th generations and an unselected control (C). A total of 69 individuals from five quail lines were genotyped by fifteen microsatellite markers. When analyzed profiles of the markers the observed ($H_o$) and expected ($H_e$) heterozygosity ranged from 0.04 (GUJ0027) to 0.64 (GUJ0087) and 0.21 (GUJ0027) to 0.84 (GUJ0037), respectively. Also, $H_o$ and $H_e$ were separated from 0.30 (L and LBW) to 0.33 (C and HBW2) and from 0.52 (HBW2) to 0.58 (L and LBW), respectively. The mean polymorphic information content (PIC) ranged from 0.46 (HBW2) to 0.52 (L). Approximately half of the markers were informative ($PIC{\geq}0.50$). Genetic distances were calculated from 0.09 (HBW1 and HBW2) to 0.33 (C and L). Phylogenetic dendrogram showed that the quail lines were clearly defined by the microsatellite markers used here. Bayesian model-based clustering supported the results from the phylogenetic tree. These results reflect that the set of studied markers can be used effectively to capture the magnitude of genetic variability in selected Japanese quail lines. Also, to identify markers and alleles which are specific to the divergence lines, further generations of selection are required.

• The Plant Pathology Journal
• /
• 제37권2호
• /
• pp.162-172
• /
• 2021

Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soybean. The natural host range for SMV is very narrow, and generally limited to Leguminosae. However, we found that SMV can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross-family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly techniques to carry out SMV full-length genome amplification from susceptible soybeans and constructed an infectious cDNA clone for SMV. The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes a polyprotein consisting of 3,067 aa. SMV-SX and SMV-XFQ008 had the highest nucleotide and amino acid sequence identities of 97.03% and 98.50%, respectively. A phylogenetic tree indicated that SMV-SX and SMV-XFQ018 were clustered together, sharing the closest relationship. We then constructed a pSMV-SX infectious cDNA clone by Gibson assembly technology and used this clone to inoculate soybean and Ailanthus altissima; the symptoms of these hosts were similar to those caused by the virus isolated from natural infected plant tissue. This method of construction not only makes up for the time-consuming and laborious defect of traditional methods used to construct infectious cDNA clones, but also avoids the toxicity of the Potyvirus special sequence to Escherichia coli, thus providing a useful cloning strategy for the construction of infectious cDNA clones for other viruses and laying down a foundation for the further investigation of SMV cross-family infection mechanisms.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권5호
• /
• pp.603-609
• /
• 2011

Telomeres are special structures at the ends of eukaryotic chromosomes. Vertebrate telomeres consist of tandem repeats of conserved TTAGGG sequence and associated proteins. Birds are interesting models for molecular studies on aging and cellular senescence because of their slow aging rates and longer life spans for their body size. In this longitudinal study, we explored the possibility of using telomeres as an age-marker to predict age in Single Comb White Leghorn layer chickens. We quantified the relative amount of telomeric DNA in isolated peripheral blood lymphocytes by the Quantitative Fluorescence in situ Hybridization technique on interphase nuclei (IQ FISH) using telomere-specific DNA probes. We found that the amount of telomeric DNA (ATD) reduced significantly with an increase in chronological age of the chicken. Especially, the telomere shortening rates are greatly increased in growing individuals compared to laying and old-aged individuals. Therefore, using the ATD values obtained by IQ FISH we established the possibility of age prediction in chickens based on the telomere theory of aging. By regression analysis of the ATD values at each age interval, we formulated an equation to predict the age of chickens. In conclusion, the telomeric DNA values by IQ FISH analyses can be used as an effective age-marker in predicting the chronological age of chickens. The study has implications in the breeding and population genetics of poultry, especially the reproductive potential.

• 한국응용곤충학회지
• /
• 제56권2호
• /
• pp.97-105
• /
• 2017

단감원에서 식나무깍지벌레의 발생을 최소화하고자 시기별 발생특성과 우수 방제약제를 선발하였다. 가지에서 월동한 약충이나 암컷 깍지는 9월 중순경에 교미 후, 월동에 들어가며 다음 해 봄철에 기온상승과 더불어 포란과 산란의 과정을 거쳐, 5월 중하순까지 산란을 마친 후 죽었다. 수컷은 대부분 9월 말까지 우화하여 암컷과 교미하고 죽었으나 낙엽에서 월동하는 개체의 경우 모두 사멸하였다. 식나무깍지벌레의 산란 특성은 매년 기상상황에 따라 차이가 있었고, 4월 중순부터 5월 중 하순까지 산란하고, 5월 초 중순에 산란 최성기를 보였다. 산란 량은 160개 정도로 추정되며, 산란 최성기로부터 약 1주일 경과 후 부화 최성기를 보였다. 여름철에는 7월 초 중순에 시작하여 8월 중 하순까지 산란하고, 7월 말과 8월 초에 산란 최성기에 도달하며, 암컷 한 마리당 산란수는 봄철보다도 다소 적은 130개 정도이고, 난 기간은 약 4일로 추정되었다. 가는 가지에서 월동한 성충이 산란한 알로부터 부화한 1세대 약충은 가지와 잎에서 각각 10%, 90% 정도의 비율로 관찰되었다. 가는 가지에서 대부분 암컷 깍지로 발육하고 2009년 7월 27일 2세대 약충 발생 최성기에 도달한 다음 8월 중 하순경부터 월동처로 정하고 살아간다. 암컷과 수컷 깍지는 8월 이전까지 잎에서 거의 비슷한 비율로 발육하다가, 2009년 8월 12일 이후 잎에서 수컷의 약충 발생 최성기를 시작으로 8월 27일 수컷 깍지벌레 발생 최성기, 9월 14일 전후로 수컷 성충의 발생에 이르기까지 일관된 발생관계를 관계를 보여주었다. 8월 12일 잎에서 관찰되는 약충 중 75% 정도는 발육하여 수컷으로 우화하여 교미하고 죽는 것으로 나타났다. Buprofezine+dinotefurn (20+15) WP로 6월 9일과 16일 2회 방제하고 7주 후에 생사충을 판정한 결과 방제가가 90.6%로 나타났다.

• 대한한의학원전학회지
• /
• 제30권3호
• /
• pp.181-195
• /
• 2017

Objectives : This paper contemplates how the special features found in Moyuan as revealed in Wenyilun has continued from the Moyuan concepts of past includig Huangdineinjing. The author of this paper attempted to distinguish common and different points between the two, and how these features changed in the following generations. Methods : The concept of Moyuan as revealed in Huangdineinjing is first reviewed before laying out the concepts of Moyuan in Wenyilun by different categories. These two concepts are then compared. A Chinese medicine literature database is used in continuing this study, and materials are selected with relation to the special features of Moyuan. Results : The special feature of half-exterior half-interior found in the Moyuan in Wenyilun is similar with that in Neijing in that they both thought Moyuan existed between the stomach, the lungs, and the five organs. The concealability of Moyuan is similar with the explanation of Moyuan in Suwen nuelun, and it is related with the story of Gaohuang in Chunqiu Zuozhuan. Compared to the fact that the concept of Moyuan in Neijing is explained as that pathogenic factor enters the surface of the body in sequence, the concept of Moyuan in Wenyilun is conveyed in that the pathogenic factor conceals itself first before being divided into exterior and interior. Moyuan is also related to upright qi, and Gaohuang-yuan: origin of Gaohuang, too, is connected Shenjiandongqi: the energy that moves between the two kidnets, and also the triple burner. Conclusions : The concepts of "Half-exterior Half-interior", concealability, and "divide it into several places in half-exterior", and upright qi contained within the Moyuan in Wenyilun are studied for their origins, and this effort is anticipated to be greatly helpful in both understanding the historical contexts of Wuyouke Wenyilun and conducting studies on clinical application of Moyuan theory.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권8호
• /
• pp.1103-1109
• /
• 2002

This study investigated the effect of 2-bromo-$\alpha$-ergocryptine (anti prolactin agent) on plasma levels of prolactin, oestradiol-17$\beta$ and progesterone in domestic hen during the active period of lay. Fifty healthy female White Leghorn birds were administered with anti prolactin agent (2-bromo-$\alpha$-ergocryptine, Sigma-USA., methane sulphonate salt, $C_{32}H_{40}BrN_5O_5.CH_4SO_3$) subcutaneously @100$\mu$g/kg body weight at weekly intervals from 17th to 36th week of age. Another group of fifty birds as controls were given placebo in place of bromocriptine. The level of prolactin remained lower in treated birds than in the control birds from 19 to 36 weeks of age. Level of prolactin even in the control group was found to decrease during the peak production period. Oestradiol-$17{\beta}$ and progesterone concentration in treated birds were significantly (p<0.01) higher than the controls during the treatment. Egg production, is positively correlated with oestradiol-$17{\beta}$ (r=0.02; r=0.67) and progesterone (r=0.49; r=0.90) in control and treated groups respectively where as prolactin level is positively correlated with egg production in the control birds (r=0.07). Prolactin levels were negatively correlated with egg production (r=-0.55) in treated birds; and oestradiol-$17{\beta}$ (r =-0.71; r=-0.53) and progesterone (r=-0.22; r=-0.27) respectively in control and treated groups. The total number of pause days during the treatment period decreased significantly (p<0.01) in the treated group compared to the control group. The reduction in pause days in treated group resulted in 1.76% increase in egg production over that in control group. The increase in egg laying days and the total egg production were found to be significant (p<0.01). These results indicate that a lower level of prolactin in circulatory blood enhances egg production in the domestic hen.

• 한국산학기술학회:학술대회논문집
• /
• 한국산학기술학회 2008년도 추계학술발표논문집
• /
• pp.484-487
• /
• 2008

본 연구는 전국 중소 상장기업을 대상으로 기업의 통합정보시스템이 업무 사용자들에게 어떠한 영향을 미치는지를 조사 분석하였다. 최근 몇 년동안 많은 중소기업들은 새로운 통합시스템을 도입 및 운영하고 있었지만 시스템의 불안정, 유지 및 관리통제의 미흡 등으로 실질적인 성과분석에 어려움을 겪고 있었다. 따라서 본 연구에서는 비교적 안정적적으로 시스템을 운영하고 있는 기업을 대상으로 통합 정보시스템을 측정하여 공통적인 문제점을 분석하고자 하였다. 이러한 분석을 위하여 가시적인 정보화 전략, 시스템 품질, 정보 품질 정보 지원요원들의 서비스 품질 등의 변수를 중심으로 연구모형과 연구가설을 설계 하였다. 연구 분석결과 정보시스템 품질, 정보 품질 정보 지원요원 들의 서비스 품질이 좋을수록 사용자 만족도에 정(긍정적)의 영향을 주는 정도가 매우 높은 것으로 분석되었다. 반면 세부 항목에서 정보화 전략은 사용자의 만족도에 큰 영향을 미치지 못하는 요인으로 나타났으며, 사용자 만족도가 높으면 개인효과에 어느 정도 영향을 미치게 된다는 결과를 얻었다. 본 연구는 향후 중소기업의 다양한 산업 분야에서 안정적인 시스템 도입에 유용한 자료가 될 수 있을 것으로 기대하고 있다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권6호
• /
• pp.976-982
• /
• 2007

This study investigated whether supplemental Cu, Fe, Zn, and Mn are needed in a practical diet for pullets. Four hundred and twenty females of an egg-laying strain (1-d-old, Lohmann Brown Layer) were randomly distributed into 4 groups, consisting of 7 replicates of 15 birds each. During the 18-week experimental period, chicks were given three basal diets in sequence, each with single or multiple Mn, Zn and Cu supplementation to improve the mineral balance gradually. In the Control, no Mn, Zn, and Cu were added; in the single Mn supplemented group (sMn) Mn was added to 120, 60, and 60 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn and Zn supplemented group (mMnZn), Mn was added to 180, 90, and 90 mg/kg and Zn was added to 120, 105, and 105 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn, Zn, Cu supplemented group (mMnZnCu), Mn, Zn, and Cu were added to the same multiple of basal Fe concentration relative to NRC (1994) recommendations. Energy and protein metabolizability were determined by subtracting energy/protein intake by energy/protein excretion (from both feces and urine) and dividing by energy/protein intake. There were no statistically significant differences between groups in terms of feed intake, final body weight or tibia length throughout the experiment. Optimal growth performance was observed in the Control, while adding trace minerals to basal diets tended to result in decreased productive performance. Protein metabolizability was increased by mMnZn and mMnZnCu treatments, but energy metabolizability was not affected. Concentrations of Mn, Zn, Cu in excreta varied greatly related to dietary content, and the retentions of Cu, Fe, Zn and Mn were all increased due to the improvement of mineral balance. Based on these results, it is suggested that the concentrations of Cu, Fe, Zn and Mn in typical basal diets used in this study were adequate for normal growth for pullets from 1 to 18 weeks of age.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권7호
• /
• pp.942-948
• /
• 2005

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.