• Title/Summary/Keyword: latex

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Extended latex proteome analysis deciphers additional roles of the lettuce laticifer

  • Cho, Won-Kyong;Chen, Xiong-Yan;Rim, Yeong-Gil;Chu, Hyo-Sub;Jo, Yeon-Hwa;Kim, Su-Wha;Park, Zee-Yong;Kim, Jae-Yean
    • Plant Biotechnology Reports
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    • v.4 no.4
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    • pp.311-319
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    • 2010
  • Lettuce is an economically important leafy vegetable that accumulates a milk-like sap called latex in the laticifer. Previously, we conducted a large-scale lettuce latex proteomic analysis. However, the identified proteins were obtained only from lettuce ESTs and proteins deposited in NCBI databases. To extend the number of known latex proteins, we carried out an analysis identifying 302 additional proteins that were matched to the NCBI non-redundant protein database. Interestingly, the newly identified proteins were not recovered from lettuce EST and protein databases, indicating the usefulness of this hetero system in MudPIT analysis. Gene ontology studies revealed that the newly identified latex proteins are involved in many processes, including many metabolic pathways, binding functions, stress responses, developmental processes, protein metabolism, transport and signal transduction. Application of the non-redundant plant protein database led to the identification of an increased number of latex proteins. These newly identified latex proteins provide a rich source of information for laticifer research.

Study for Reducement of Polymerization Time and Improvement of Stability in Manufacturing Carboxylated Styrene-butadiene Latex (카르복실화 스티렌-부타디엔 라텍스의 중합시간 단축과 안정성 개선을 위한 연구)

  • Cho, Ur-Ryong
    • Elastomers and Composites
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    • v.37 no.1
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    • pp.31-38
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    • 2002
  • Polymerization of carboxylated styrene-butadiene latex takes longer time than that of acrylic emulsion due to delocalization of radical in butadiene unit having conjugated double bond. A latex stability is the most important properties owing to use intact without separating polymer from base latex. For reducing polymerization time without decreasing any properties of latex, carbon tetra-chloride which has been used as the most popular chain transfer agent was replaced to combination of tert-dodecylmercaptane and ${\alpha}$-methylstyrene dimer. The replacement yielded reducement or 2 hr in polymerization time. In the increment step, charge amount of acrylic acid was limited to 0.3 part to restrain viscosity enhancement. Just after initial step, addition of 0.1 part acrylamide prevent polymer chain from diffusing between two region followed by giving hardness and final good adhesive force to latex particles.

Development of diagnostic kit(Test-MT) for the microplate latex agglutination test of toxoplasmosis in animal (Latex 응집반응을 이용한 동물의 톡소플라즈마병 진단용 kit 개발에 관한 연구)

  • Suh, Myung-deuk;Joo, Hoo-don;Maass, Daivd
    • Korean Journal of Veterinary Research
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    • v.35 no.3
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    • pp.583-593
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    • 1995
  • The present study was conducted to develop a toxoplasma latex agglutination test antigen(Test-MT) and evaluate the toxoplasma latex agglutination(LA) test using a newly-made "Test-MT kit" by comparing with the Toxo-MT kit(Eiken chemical co, Tokyo). Also, the specifity and sensitivity test were made by comparing with IFA test and IgG-ELISA. Tachyzoite suspensions of Toxoplasma gondii(RH strain) were ultracentrifuged for 30min at $60,000{\times}g(4^{\circ}C)$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $1.0{\mu}m$ in diameter(Polyscience co) were used for the preparation of sensitized latex-antigen supension(Test-MT). The frequency distribution of LA titers in Test-MT showed two peaks at <1:32 and 1:128. The borderline titer for positive test in Test-MT was determined to be 1:64. But the frequency distribution of LA tites in Toxo-MT showed two peaks at <1:16 and 1:64. The positive borderline was determined to be 1:32. Agreement of reactions between Test-MT and Toxo-MT kit by LA test was shown 92.5% in bovine sera and 97.0% in swine sera, respectively. From the results obtained here it was determined that the sensitized latex-antigen, Test-MT kit, for the microtiter agglutination test prepared as same as by the procedure described in the previous paper(Suh and Lee, 1993) was useful as a highly specific, sensitive and stable immunotiteration reagent for serodiagnosis of toxoplasma infection in animal sera.

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Drying Shrinkage Properties of Latex Modified Concrete (라텍스개질 콘크리트의 건조수축특성)

  • 이훈재;김태경;김동호;김성환;윤경구
    • Proceedings of the Korea Concrete Institute Conference
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    • 2002.05a
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    • pp.151-156
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    • 2002
  • Drying shrinkage cracking which may be caused by the relatively large specific surface is a matter of grave concern for latex modified concrete(LMC) overlay and rapid-setting cement latex modified concrete(RSLMC) overlay. LMC and RSLMC were studied for field applications very actively in terms of strength and durability in Korea. However, there were no considerations in drying shrinkage. Therefore, the purpose of this study was to investigate the drying shrinkage properties of LMC and RSLMC with the main experimental variables such as cement types(ordinary portland cement, rapid setting cement), latex contents(0, 5, 10, 15, 20%), W-C ratios, and curing days at a controlled environment of 60% of relative humidity and 2$0^{\circ}C$ of temperature. The drying shrinkage for specimens was measured with a digital dial gauge of Demec. The test results showed that the drying shrinkage of LMC and RSLMC were considerably lower than that of OPC and RSC, respectively. This might be attributed to the interlocking of hydrated cement and aggregates by a film of latex particles, water retention due to hydrophobic, and colloidal properties of the latexes resulting in reduced water evaporation.

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Characteristics of High Early Strength Latex Modified Sprayed-Mortar (조강형 라텍스개질 스프레이 모르타르의 특성)

  • Yun, Kyong-Ku;Lee, Bong-Hak;Lee, Jin-Beom
    • Journal of Industrial Technology
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    • v.33 no.A
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    • pp.93-99
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    • 2013
  • Shotcrete has been referred to as gunite, pneumatically applied mortar or concrete, sprayed concrete or mortar. There are sound reasons why sprayed mortar is one of the best portland cement based material for repairing old concrete structures. However, it is difficult to find the research results on the latex-modified mortar nevertheless on the impact of air onto the fresh and hardened properties of latex-modified mortar. So, the main experimental program included strength test, slump test, rapid chloride permeability test, image analysis for air void system, and chemical attacks with the main experimental variables of latex content, fine aggregate content, water-cement ratio, and air foamer content.

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Preparation and rheological behavior of polystyrene/multi-walled carbon nanotube composites by latex technology

  • Woo, Dong-Kyun;Kim, Byung-Chul;Lee, Seong-Jae
    • Korea-Australia Rheology Journal
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    • v.21 no.3
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    • pp.185-191
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    • 2009
  • Polystyrene/multi-walled carbon nanotube (PS/MWCNT) composites were prepared by the use of latex technology. The monodisperse PS latex was synthesized by an emulsifier-free emulsion polymerization from styrene/potassium persulfate/water system in the presence of ethanol. The MWCNTs were first treated with acid mixture to eliminate impurities, dispersed in deionized water driven by ultrasonicator, and then mixed with the PS latex. From these mixtures, PS/MWCNT composites were prepared by freeze-drying and subsequent compression molding. In the small-amplitude oscillatory shear experiments, both complex viscosity and storage modulus increased with increasing MWCNT content. A pronounced effect of MWCNT content was observed, resulting in larger storage modulus and stronger yield behavior at low frequencies when compared to unmodified PS. It showed a transition from viscous to elastic behavior with increasing MWCNT content. Over the MWCNT content of 3 wt%, the storage modulus was higher than the loss modulus across all frequencies.

Effect of Binder on Coating Layer Structure and Surface Strength of Coated Paper (바인더가 도공층 구조 및 도공지의 표면 강도에 미치는 영향)

  • 이용규;황석우
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.30 no.3
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    • pp.63-72
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    • 1998
  • This research was intended to evaluate the effect of carboxymethylcellulose(CMC) on the coating structure and surface strength of coated paper prepared with amphoteric latex based coating color. Printability and optical properties of coated papers were compared. The influence of the consolidation behavior of coating color on the coating structure and the surface strength of coated paper was investigated. Compared with the conventional anionic latex, amphoteric latex formed bulkyer, smoother and more porous coating layer, which in turn, restricted binder migration in the coating layers, and facilitated immobilization of coating colors. However, dry pick strength of coated paper was decreased. The addition of CMC to these systems had strongly influenced on. the consolidation behavior and porosity in the dry state, through forming the network structure of coating layers by the interaction with amphoteric latex particles. Thus, printability and optical properties of coated papers were improved. Results indicated that amphoteric latex could be practically applied to the paper coating to improve printability and optical properties of coated papers.

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The Effects of Interfacial Properties of the Styrene/Water on the Styrene Latex Particle Properties using Triton X-100/SDS Surfactant Mixture (폴리스티렌 라텍스 제조에 있어서 Triton X-100/SDS 계면활성제 혼합이 단량체/수용액 간의 계면물성 및 라텍스의 특성에 미치는 영향)

  • Park, A-Reum-Yi;Kim, Young-Ho
    • Journal of the Korean Applied Science and Technology
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    • v.27 no.3
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    • pp.240-248
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    • 2010
  • The blending effects of surfactants on the polystyrene emulsion polymerization were studied. The blending of Triton X-100 and SDS affects to the interfacial properties of the styrene monomer and water phases, and finally, the properties of the polystyrene latex particles. As the blending ratio of SDS/Triton X-100 increases, the interfacial tension and CMC of the blended surfactants were decreased and results in a reducing the size of the latex particles. It was found that the interfacial tension was reduced when the surfactant were blended. By increasing the SDS content, the interfacial tension was reduced, and, at a certain condition, the interfacial tension was reached to an extremely low value to form micro-emulsion and the nano-sized latex particles (80~110 nm).

A Study on the Properties of Latex Modified Concrete using Recycled Coarse Aggregate (재생굵은골재를 이용한 라텍스 개질 콘크리트의 특성에 관한 연구)

  • Yoo, Deok-Ryong;Go, Seong-Seok
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.10 no.5
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    • pp.147-156
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    • 2006
  • This study is purposed to improve the performance of concrete made of recycled coarse aggregate. For this, recycled aggregate concrete was produced with SBR latex, and fluidity, dynamic performance and drying shrinkage were examined. According to the result, with mixing 6% of SBR latex, fluidity having resistance against segregation can be insured and compressive and flexural strength was increased. Especially the increment in terms of flexures was remarkable. In addition to, with above mixing ratio, drying shrinkage was reduced. Therefore there is a strong inference that superior recycled aggregate concrete can be produced with using 6% of SBR latex.

Serological Detection of Plant Viruses with latex-test (Latex-Test에 의한 식물 바이러스의 검정)

  • 박은경;김정화;이영근
    • Journal of the Korean Society of Tobacco Science
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    • v.1 no.1
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    • pp.33-38
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    • 1979
  • A simple test using latex-agglutination was developed to detect serologically tobacco mosaic virus (TMV), soybean mosaic virus (SoyMV), and barley stripe mosaic virus (BSMV) from infected Plants. Latex spheres ( 0.81 $\mu$, Difco) were adsorbed with immuno globulin purified by electrophoresis from crude antiserum against viruses. The antibody- sensitized latex suspension was mixed with sap from virus -infected leaves in a glass capillary tube (inner diam. 1mm $\times$ 100 mm length) The mixture, after agitation, was observed under a stereo microscope at low magnification (X20 - X4O), to examine the reaction between antigen (Virus) and its antibody. Flocculation occurred when the reaction was positive.

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