Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Extended latex proteome analysis deciphers additional roles of the lettuce laticifer

  • Cho, Won-Kyong (Division of Applied Life Science (BK21 Program), Environmental Biotechnology, National Core Research Center, PMBBRC) ;
  • Chen, Xiong-Yan (Division of Applied Life Science (BK21 Program), Environmental Biotechnology, National Core Research Center, PMBBRC) ;
  • Rim, Yeong-Gil (Division of Applied Life Science (BK21 Program), Environmental Biotechnology, National Core Research Center, PMBBRC) ;
  • Chu, Hyo-Sub (Division of Applied Life Science (BK21 Program), Environmental Biotechnology, National Core Research Center, PMBBRC) ;
  • Jo, Yeon-Hwa (Division of Applied Life Science (BK21 Program), Environmental Biotechnology, National Core Research Center, PMBBRC) ;
  • Kim, Su-Wha (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Park, Zee-Yong (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Kim, Jae-Yean (Division of Applied Life Science (BK21 Program), Environmental Biotechnology, National Core Research Center, PMBBRC)
  • Received : 2010.03.18
  • Accepted : 2010.08.18
  • Published : 2010.12.30
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Abstract

Lettuce is an economically important leafy vegetable that accumulates a milk-like sap called latex in the laticifer. Previously, we conducted a large-scale lettuce latex proteomic analysis. However, the identified proteins were obtained only from lettuce ESTs and proteins deposited in NCBI databases. To extend the number of known latex proteins, we carried out an analysis identifying 302 additional proteins that were matched to the NCBI non-redundant protein database. Interestingly, the newly identified proteins were not recovered from lettuce EST and protein databases, indicating the usefulness of this hetero system in MudPIT analysis. Gene ontology studies revealed that the newly identified latex proteins are involved in many processes, including many metabolic pathways, binding functions, stress responses, developmental processes, protein metabolism, transport and signal transduction. Application of the non-redundant plant protein database led to the identification of an increased number of latex proteins. These newly identified latex proteins provide a rich source of information for laticifer research.

Keywords

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