• Title/Summary/Keyword: lateral flow test

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Counter-Current Air-Water Flow in Narrow Rectangular Channels With Offset Strip Fins

  • Kim, Byong-Joo;Sohn, Byung-Hu;Koo, Kee-Kahb
    • Journal of Mechanical Science and Technology
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    • v.17 no.3
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    • pp.429-439
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    • 2003
  • Counter-current two-phase flows of air- water in narrow rectangular channels with offset strip fins have been experimentally investigated in a 760 mm long and 100 mm wide test section with 3.0 and 5.0 mm gap widths. The two-phase flow regime, channel-average void fractions and two-phase pressure gradients were studied. Flow regime transition occurred at lower superficial velocities of air than in the channels without fins. In the bubbly and slug flow regimes, elongated bubbles rose along the subchannel formed by fins without lateral movement. The critical void fraction for the bubbly-to-slug transition was about 0.14 for the 3 mm gap channel and 0.2 for the 5 mm gap channel. respectively. Channel-average void fractions in the channels with fins were almost the same as those in the channels without fins. Void fractions increased as the gap width increased, especially at high superficial velocity of air. The presence of fins enhanced the two-phase distribution parameter significantly in the slug flow, where the effect of gap width was almost negligible. Superficial velocity of air dominated the two-phase pressure gradients. Liquid superficial velocity and channel gap width has only a minor effect on the pressure gradients.

An Experimental Study on the Estimation of Optimum Length of Soil Flow Protector with Wall Stiffness (벽체 강성에 따른 토사유입차단판의 최적 길이 산정에 관한 실험적 연구)

  • Yoo, Jae-Won;Seo, Min-Su;Son, Su-Won;Im, Jong-Chul
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.39 no.6
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    • pp.789-799
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    • 2019
  • The settlement hardly occurs in structures supported by pile foundation such as abutment, culvert but a cavity is formed in the lower part of a structure. As a result, soil discharged from the lateral ground to the cavity accelerates the settlement of the lateral ground of the structure, resulting in a larger settlement. Therefore, in order to prevent problems caused by cavity under the structure supported by pile foundation, soil Flow Protector (briefly called 'FLP'), which can be easily installed on the side of structure, was developed. In this study, an laboratory model test was carried out to prove the reduction effect of settlement and to estimate the optimal installation length of the FLP. As a result, the installation of the FLP reduced the settlement of the lateral ground and prevented the leakage of lateral ground soil into the cavity. If the stiffness of the FLP is small, the state or active earth pressure is generated in the upper part, which is not favorable for stability. But if the stiffness of the FLP is high enough, the passive earth pressure area is generated in the upper part, which will be advantageous for the stability. Also, the increased installation length of FLP is effective to reduce the settlement. And the ratio of the optimal length of the FLP to the box structure height (H = 250 mm) are flexible FLP 1.38, stiff FLP 0.73.

Lateral Stress and Pore Pressure During One-dimensional Consolidation of Clay (점토의 일차원 압밀과정중 작용하는 수평토압과 간극수압)

  • 김재영
    • Journal of the Korean Geotechnical Society
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    • v.20 no.6
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    • pp.5-10
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    • 2004
  • The earth pressure coefficient at rest for clayey soils in the one-dimensional state, $K_0$ obtained from the triaxial test is not correct in principle because the seepage flow is radial and the displacement of soil elements is three-dimensional. Measurements of the earth pressure and the pore water pressure during one-dimension consolidation in the consolidometer ring are presented. The earth pressure and pore water pressure are measured directly by a circular part of the consolidometer ring of a floating type at its mid height. A plastic clay showed $K_0$=0.5 irrespective of pressure in the consolidometer ring.

The Behavior of Piled Bridge Abutments Subjected to Lateral Soil Movements - A Study on the Centrifuge Model Tests - (측방유동을 받는 교대말뚝기초의 거동분석 (I) - 원심모형실험 연구 -)

  • 서정주;서동희;정상섬;김유석
    • Journal of the Korean Geotechnical Society
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    • v.19 no.1
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    • pp.5-19
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    • 2003
  • A series of centrifuge model tests were conducted to investigate the behavior of piled bridge abutments subjected to lateral soil movements induced by approach embankments. The effect of clay layer depth and the rate of embankment construction on piled bridge abutments are the main focus of this study. Tests were performed for two loading types: (1) incremental loading applied in six lifts to the final embankment height; (2) instant loading corresponding to the final embankment height applied in one lift quickly. A variety of instrumentations such as LVDTs, strain gauges, earth pressure transducers, and pore pressure transducers are installed in designed positions in order to clarify the soil-pile interaction and the short- and long-term behavior for piled bridge abutments adjacent to surcharge loads. Based on the results of a series of centrifuge model tests, the distribution of lateral flow induced by staged embankment construction has trapezoidal distribution. The maximum lateral soil pressure is about 0.75$\gamma$H at surcharge loading stage, and about 0.35 $\gamma$H at over 80% consolidated stage.

Development of Nucleic Acid Lateral Flow Immunoassay for Rapid and Accurate Detection of Chikungunya Virus in Indonesia

  • Ajie, Mandala;Pascapurnama, Dyshelly Nurkartika;Prodjosoewojo, Susantina;Kusumawardani, Shinta;Djauhari, Hofiya;Handali, Sukwan;Alisjahbana, Bachti;Chaidir, Lidya
    • Journal of Microbiology and Biotechnology
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    • v.31 no.12
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    • pp.1716-1721
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    • 2021
  • Chikungunya fever is an arboviral disease caused by the Chikungunya virus (CHIKV). The disease has similar clinical manifestations with other acute febrile illnesses which complicates differential diagnosis in low-resource settings. We aimed to develop a rapid test for CHIKV detection based on the nucleic acid lateral flow immunoassay technology. The system consists of a primer set that recognizes the E1 region of the CHIKV genome and test strips in an enclosed cassette which are used to detect amplicons labeled with FITC/biotin. Amplification of the viral genome was done using open-source PCR, a low-cost open-source thermal cycler. Assay performance was evaluated using a panel of RNA isolated from patients' blood with confirmed CHIKV (n = 8) and dengue virus (n = 20) infection. The open-source PCR-NALFIA platform had a limit of detection of 10 RNA copies/ml. The assay had a sensitivity and specificity of 100% (95% CI: 67.56% - 100%) and 100% (95% CI: 83.89% - 100%), respectively, compared to reference standards of any positive virus culture on C6/36 cell lines and/or qRT-PCR. Further evaluation of its performance using a larger sample size may provide important data to extend its usefulness, especially its utilization in the peripheral healthcare facilities with scarce resources and outbreak situations.

Development of a lateral flow dipstick test for the detection of 4 strains of Salmonella spp. in animal products and animal production environmental samples based on loop-mediated isothermal amplification

  • Wirawan Nuchchanart;Prapasiri Pikoolkhao;Chalermkiat Saengthongpinit
    • Animal Bioscience
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    • v.36 no.4
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    • pp.654-670
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    • 2023
  • Objective: This study aimed to develop loop-mediated isothermal amplification (LAMP) combined with lateral flow dipstick (LFD) and compare it with LAMP-AGE, polymerase chain reaction (PCR), and standard Salmonella culture as reference methods for detecting Salmonella contamination in animal products and animal production environmental samples. Methods: The SalInvA01 primer, derived from the InvA gene and designed as a new probe for LFD detection, was used in developing this study. Adjusting for optimal conditions by temperature, time, and reagent concentration includes evaluating the specificity and limit of detection. The sampling of 120 animal product samples and 350 animal production environmental samples was determined by LAMP-LFD, comparing LAMP-AGE, PCR, and the culture method. Results: Salmonella was amplified using optimal conditions for the LAMP reaction and a DNA probe for LFD at 63℃ for 60 minutes. The specificity test revealed no cross-reactivity with other microorganisms. The limit of detection of LAMP-LFD in pure culture was 3×102 CFU/mL (6 CFU/reaction) and 9.01 pg/μL in genomic DNA. The limit of detection of the LAMP-LFD using artificially inoculated in minced chicken samples with 5 hours of pre-enrichment was 3.4×104 CFU/mL (680 CFU/reaction). For 120 animal product samples, Salmonella was detected by the culture method, LAMP-LFD, LAMP-AGE, and PCR in 10/120 (8.3%). In three hundred fifty animal production environmental samples, Salmonella was detected in 91/350 (26%) by the culture method, equivalent to the detection rates of LAMP-LFD and LAMP-AGE, while PCR achieved 86/350 (24.6%). When comparing sensitivity, specificity, positive predictive value, and accuracy, LAMP-LFD showed the best results at 100%, 95.7%, 86.3%, and 96.6%, respectively. For Kappa index of LAMP-LFD, indicated nearly perfect agreement with culture method. Conclusion: The LAMP-LFD Salmonella detection, which used InvA gene, was highly specific, sensitive, and convenient for identifying Salmonella. Furthermore, this method could be used for Salmonella monitoring and primary screening in animal products and animal production environmental samples.

A Study on Behavior Characteristics of Soft Ground by DCM Arrangement Type (DCM 배치 형상에 따른 연약지반 거동 특성에 관한 연구)

  • You, Seung-Kyong;Lee, Jong-Young;Hong, Gigwon
    • Journal of the Korean Geosynthetics Society
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    • v.20 no.4
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    • pp.125-131
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    • 2021
  • This study described the relationship of settlement-lateral displacement and settlement-heaving according to the DCM type using the model test results, in order to evaluate the behavioral characteristics of the soft ground improved with DCM. As a result, it was found that the total settlement of the model ground was relatively small in the soft ground, to which the DCM was applied, and the settlement was less in the order of the grid type, wall type, and pile type under the same load conditions. This trend was also the same for the lateral displacement and heaving. In addition, the relationship between settlement and lateral displacement of soft ground was analyzed to be similar to that of previous study (Leroueil et al., 1990). Therefore, the DCM of grid type was evaluated to be superior to other types for lateral flow and heaving in the improvement effect of soft ground.

Development of Reservoir Operation Model using Simulation Technique in Flood Season (I) (모의기법에 의한 홍수기 저수지 운영 모형 개발 (I))

  • Sin, Yong-No;Maeng, Seung-Jin;Go, Ik-Hwan;Lee, Hwan-Gi
    • Journal of Korea Water Resources Association
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    • v.33 no.6
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    • pp.745-755
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    • 2000
  • The dam operation system of KOWACO for flood control doesn't have capability to account for the downstream hydrologic conditions and any feasible index to decide the pre-release from the forecasted rainfall and inflow. In this study, a dam operation model for flood control was developed to account for the flood flow condition of its downstream to give users the dam release schedules. Application test of EV ROM to Keum River showed that EV ROM is superior to the Rigid ROM and Technical ROM which are currently used by KOWACO. EV ROM developed in this study provides a release schedule accounting for the cumulative lateral flow hydrograph at the downstream control points where the discharge does not depend only on the dam operation. but also on lateral inflow from the tributaries. In order to reduce the peak discharge at the control points, it suggests the preliminary release during the early rising phase of the predicted hydrograph, holding the flood flow inside the dam during a peak phase, and afterward resuming the release. Three case studies of flood control by the operation of Daechung Multipurpose Dam in Geum River Basin show that the EV ROM is superior to the Rigid ROM and Technical ROM. This must be due to its nature to account for the downstream flow condition as well as the inflow and water level of the dam. It was also conceived that further case studies of EV ROM and more accurate rainfall prediction would improve the dam operation for flood control.ontrol.

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Experimental Investigation of Unsteady Pressure Generated by Oscillating Outer Cylinder (진동하는 외부 실린더에 의해 유발되는 비정상 압력의 실험적 고찰)

  • 심우건
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.2
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    • pp.519-526
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    • 1995
  • Experiments have been performed to test the analytical tools developed concurrently for the motion-related unsteady pressure in annular passages. The outer cylindrical body was oscillated by a shaker in either rocking motion about a hinge-point or lateral translation motion. In the equilibrium configuration the two bodies are either concentric or eccentric, in the plane of oscillation or normal to it. The unsteady pressure generated by the oscillatory motion with low amplitudes (displacement/radius) was measured on wall of the fixed inner cylinder at various axial and azimuthal locations. The unsteady pressure were compared with theoretical predictions, and agreement was found to be within 10%. Experiments have been shown that the effect of flow velocity on the unsteady pressure is minimal and the pressure increases more or less with oscillatory motion, for low flow velocities (Re = 2 900).

Application of EPS Considering Long-term Durability (장기내구성을 고려한 EPS의 현장 적용성)

  • Chun, Byungsik;Jung, Changhee;Ahn, Jinhyun
    • Journal of the Korean GEO-environmental Society
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    • v.8 no.6
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    • pp.53-60
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    • 2007
  • L/EPS, manufactured in the shape of block and used for civil engineering, is a lightweight material with an excellent resistance to compression, and provides a superb self-sufficient stability. EPS is a suitable material capable of resolving the problem of settlement and lateral flow if it is applied as the soil on soft ground. The Korean Standards (KS) has not yet proposed any testing method for use of EPS as an engineering banking material. Only its testing and quality ordinance as a heat insulation material has been standardized. The design criteria for EPS has been established and applied through the trial construction of KHC (Korea Highway Corporation) and quality test of manufacturer, but most studies on them have been confined to factory products. This study is focused on comparing and analyzing long-term durability by conducting cyclic load test, freezing and thawing test, absorption rate test and others. EPS used in the test was chosen from construction sites and factory products, focusing on the long-term durability of EPS depending on the passage of time. Unconfined compression test results indicated that the strength of collected samples was lower than factory products. While the triaxial compression test results indicated that the shear strength increased in proportion to the increase of confining pressure, and factory products had declining shear strength as the confining pressure rose.

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