• Title/Summary/Keyword: late blight

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Antifungal activities for derivatives of 4-isopropyl-3-methylphenol and 5-isopropyl-3-methylphenol against plant pathogenic fungi (4-Isopropyl, 5-isopropyl-3-methylphenol 유도체들의 합성과 식물 병원균에 대한 항균 활성)

  • Choi, Won-Sik;Jang, Soon-Ho;Jang, Do-Yeon;Choi, Kyoung-Gil;Lee, Byung-Ho;Kim, Tae-Jun;Jung, Bong-Jin
    • The Korean Journal of Pesticide Science
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    • v.10 no.4
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    • pp.249-261
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    • 2006
  • Fifty compounds such as ester, sulfonyl ester, carbamate, ether and phosphoyl ester derivatives of 4-isopropyl-3-methylphenol(I) and 5-isopropyl-3-methylphenol(II) were synthesized. These derivatives were identified by IR, GC/MS and $^1H$-NMR spectra. Their in vitro antifungal activities were tested against 10 plant pathogenic fungi. Among them, several compounds showed potent in vitro antifungal activity. The selected compounds showing potent in vitro antifungal activity were tested for their in vivo antifungal activities against 5 plant diseases such as rice blast, rice sheath blast, cucumber anthracnose, cucumber gray mold and tomato late blight. As a result, 4-isopropyl-3-methylphenyl(2-amino-thiazole-4-yl)methoxyiminoacetate(I-7a) showed a potent in vivo antifungal activity against rice blast. Both methyl (4-isopropyl-3-methylphenoxy)acetate(I-4d) and methyl (5-isopropyl-3-methylphenoxy)acetate(II-4d) effectively inhibited the development of cucumber gray mold.

Isolation of an Rx homolog from C. annuum and the evolution of Rx genes in the Solanaceae family

  • Shi, Jinxia;Yeom, Seon-In;Kang, Won-Hee;Park, Min-Kyu;Choi, Do-Il;Kwon, Jin-Kyung;Han, Jung-Heon;Lee, Heung-Ryul;Kim, Byung-Dong;Kang, Byoung-Cheorl
    • Plant Biotechnology Reports
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    • v.5 no.4
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    • pp.331-344
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    • 2011
  • The well-conserved NBS domain of resistance (R) genes cloned from many plants allows the use of a PCR-based approach to isolate resistance gene analogs (RGAs). In this study, we isolated an RGA (CapRGC) from Capsicum annuum "CM334" using a PCR-based approach. This sequence encodes a protein with very high similarity to Rx genes, the Potato Virus X (PVX) R genes from potato. An evolutionary analysis of the CapRGC gene and its homologs retrieved by an extensive search of a Solanaceae database provided evidence that Rx-like genes (eight ESTs or genes that show very high similarity to Rx) appear to have diverged from R1 [an NBS-LRR R gene against late blight (Phytophthora infestans) from potato]-like genes. Structural comparison of the NBS domains of all the homologs in Solanaceae revealed that one novel motif, 14, is specific to the Rx-like genes, and also indicated that several other novel motifs are characteristic of the R1-like genes. Our results suggest that Rx-like genes are ancient but conserved. Furthermore, the novel conserved motifs can provide a basis for biochemical structural. function analysis and be used for degenerate primer design for the isolation of Rx-like sequences in other plant species. Comparative mapping study revealed that the position of CapRGC is syntenic to the locations of Rx and its homolog genes in the potato and tomato, but cosegregation analysis showed that CapRGC may not be the R gene against PVX in pepper. Our results confirm previous observations that the specificity of R genes is not conserved, while the structure and function of R genes are conserved. It appears that CapRGC may function as a resistance gene to another pathogen, such as the nematode to which the structure of CapRGC is most similar.

A New Potato Cultivar "Jayoung", with High Concentration of Anthocyanin (Anthocyanin 함량이 높은 감자 신품종 "자영")

  • Park, Young Eun;Cho, Ji Hong;Cho, Hyun Mook;Yi, Jung Yun;Seo, Hyo Won;Chung, Myoung Gun
    • Korean Journal of Breeding Science
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    • v.41 no.1
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    • pp.51-55
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    • 2009
  • Jayoung was bred within the potato breeding program of Highland Agriculture Research Center (HARC), National Institute of Crop Science. It was originated from a cross made between Atlantic as a female and AG34314 as a male parent in 2003. Jayoung was evaluated as a clone number Daegwan 1-104 for the growth and tuber characteristics, yielding ability, and resistance to major potato diseases in the regional yield trials from 2006 to 2007. Finally, this clone was renamed as Jayoung and was presented to Korean Seed & Variety Service in 2007 for registration as a new potato cultivar. Jayoung has a oval shape, shallow eye depth, dark purple skinned and fleshed tuber characteristics. Its mean tuber yields from the regional yield trials was 37.3 MT/ha, and it showed the highest yield in spring cropping. Jayoung showed high resistance to both common scab (Streptomyces scabies) and potato leaf roll virus (PLRV), however it was susceptible to late blight (Phytophthora infestans). The dry matter content of Jayoung was fairy high 20.3%, therefore it is suitable for both chip processing and table usage. Its anthocyanin content was the highest in autumn cropping as 43.7 mg/100 g fresh weight and its mean anthocyanin content was 4.7 times higher than that of Jasim.

A New High Amylose Rice Variety "Goamibyeo" (아밀로스함량이 높은 벼 품종 "고아미벼")

  • Song, You-Chun;Lim, Sang-Jong;Lee, Jeom-Sig;Kim, Ho-Yeong;Yeo, Un-Sang;Park, No-Bong;Kwak, Do-Yeon;Kang, Jong-Rae;Yang, Sae-Jun;Hwang, Hung-Goo;Oh, Byeong-Gen;Moon, Huhn-Pal;Lim, Moo-Sang
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.447-451
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    • 2008
  • 'Goamibyeo', a mid-late maturing ecotype with high amylose content in kernels, was developed by the rice breeding team of National Yeongnam Agricultural Experiment Station(NYAES) in 2000 and released in 2001. This variety was derived from the three way cross of Milyang 95//Kimcheonaengmi/$2^*Ilpumbyeo$ (in 1992 summer) through the pedigree breeding method and designated as 'Milyang 168' in 1997. The heading date of 'Goamibyeo' was Aug. 18 in ordinary season with culm length of 85 cm. However, 'Goamibyeo' showed susceptible to bacterial leaf blight, stripe virus and leaf blast disease. The amylose content of 'Goamibyeo' in milled rice kernels is about 26.7% with translucent and clear in chalkness. Thus, 'Goamibyeo' is expected to be used as a source grain for rice noodle industries. The milled rice yield potential of 'Goamibyeo' is about 5.38 MT/ha in local adaptability test of three years and it would be adaptable to Yeongnam plain of Korea.

A New Potato Cultivar "Hongyoung", with Red Skin and Flesh Color, and High Concentrations of Anthocyanins (껍질과 속색이 붉고 Anthocyanin 함량이 높은 감자 "홍영")

  • Park, Young Eun;Cho, Ji Hong;Cho, Hyun Mook;Yi, Jung Yun;Seo, Hyo Won;Choung, Myoung Gun
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.502-506
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    • 2009
  • "Hongyoung" was bred within the potato breeding program of National Institute of Highland Agriculture (NIHA). It was originated from a cross made between Atlantic as a female and AG34314 as a male parent in 2003. "Hongyoung" was evaluated as a clone number Daegwan 1-102 for the growth and tuber characteristics, yielding ability, and resistance to major diseases in the regional yield trials from 2006 to 2007. Finally, this clone was renamed as "Hongyoung" and was presented to Korean Seed & Variety Service in 2007 for registration as a new potato cultivar. "Hongyoung" has a round shape, shallow eye depth, red skinned and fleshed tuber characteristics. Its mean tuber yields from the regional yield trials was 37.4 MT/ha, and it showed the highest yield in spring cropping. "Hongyoung" showed high resistance to common scab (Streptomyces scabies) and potato leaf roll virus (PLRV), however it was susceptible to both potato virus Y (PVY) and late blight (Phytophthora infestans). The dry matter content of "Hongyoung" was low to medium 17.7%, therefore it is suitable for table use. Its anthocyanin content was the highest in autumn cropping with 31.8 mg/100g fresh weight and it contained 3.6 times higher anthocyanin than the control, Jasim.

Weather Effect and Response of Promoted Rice Varieties on Fusarium Infection in Paddy Field (벼 붉은곰팡이병 감염에 대한 기상조건의 영향과 장려품종의 반응)

  • Lee, Theresa;Jang, Ja Yeong;Kim, Jeomsoon;Ryu, Jae-Gee
    • Research in Plant Disease
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    • v.24 no.4
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    • pp.313-320
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    • 2018
  • Fusarium infection rate of the paddy rice grain after harvest seemed to be influenced by the average temperature from late July (before heading) to the end of September (during ripening). In case of 2010 and 2013 in which average temperature of the same period was similar, Fusarium infection was related to cumulative precipitation, cumulative precipitation days, and precipitation durations over two days. The distribution ratio of Fusarium species complex isolated from paddy rice grains after harvest was 57% in 2010 and 45% in 2013 for Fusarium graminearum species complex (FGSC), 35% and 50% for Fusarium incarnatum-equiseti species complex, and 8% and 5% for Fusarium fujikuroi species complex (FFSC). The distribution ratios of FGSC and FFSC were higher in 2010 than 2013. Among the total 26 promoted rice varieties, the 'Mihyang' showed resistant response against the natural infection with Fusarium species belonging to FGSC and the varieties of 'Nampyeong', 'Hi-ami'and 'Younghojinmi' showed resistant response against the natural infection with overall Fusarium pathogens. Majority of the promoted rice varieties could not be classified for resistance or susceptibility. These results are valuable as basic data to determine the resistance and susceptibility of rice variety against Fusarium spp. infection in the field.

PCR-based markers to select plastid genotypes of Solanum acaule (Solanum acaule 색소체 유전자형 선발을 위한 특이적 분자마커 개발)

  • Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.178-186
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    • 2022
  • The tetraploid Solanum acaule is a wild potato species from Bolivia widely used for potato breeding because of its diverse attractive traits, including resistance to frost, late blight, potato virus X, potato virus Y, potato leafroll virus, potato spindle tuber viroid, and cyst nematode. However, the introgression of useful traits into cultivated potatoes via crossing has been limited by differences in endosperm balance number between species. Somatic fusion could be used to overcome sexual reproduction barriers and the development of molecular markers is essential to select proper fusion products. The chloroplast genome of S. acaule was sequenced using next-generation sequencing technology and specific markers for S. acaule were developed by comparing the obtained sequence with those of seven other Solanum species. The total length of the chloroplast genome is 155,570 bp, and 158 genes were annotated. Structure and gene content were very similar to other Solanum species and maximum likelihood phylogenetic analysis with 12 other species belonging to the Solanaceae family revealed that S. acaule is very closely related to other Solanum species. Sequence alignment with the chloroplast genome of seven other Solanum species revealed four InDels and 79 SNPs specific to S. acaule. Based on these InDel and SNP regions, one SCAR marker and one CAPS marker were developed to discriminate S. acaule from other Solanum species. These results will aid in exploring evolutionary aspects of Solanum species and accelerating potato breeding using S. acaule.

A Late-Maturing and Whole Crop Silage Rice Cultivar 'Mogwoo' (만생종 총체사료용 벼 신품종 '목우')

  • Lee, Sang-Bok;Yang, Chang-Ihn;Lee, Jeom-Ho;Kim, Myeong-Ki;Shin, Young-Seop;Lee, Kyu-Seong;Choi, Yong-Hwan;Jeong, O-Young;Jeon, Yong-Hee;Hong, Ha-Cheol;Kim, Yeon-Gyu;Jung, Kuk Hyun;Jeung, Ji-Ung;Kim, Junhwan;Shon, Ji-Young
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.81-86
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    • 2013
  • 'Mogwoo', a new high yield and whole crop silage rice (Oryza sativa L.) cultivar, was developed by the rice breeding team of the National Institute of Crop Science, RDA, Suwon, Korea, from 1999 to 2009, and was released in 2010. It was derived in 1999 from a cross between Dasanbyeo, having a high yield, and Suweon431/IR71190-45-2-1. A promising line, SR25848-C99-1-2-1, selected by the pedigree breeding method, was designated the name of 'Suweon 519' in 2007. This cultivar has about 155 days of growth period from seeding to heading, and is tolerance to lodging, with erect pubescent leaves as well as a long and thick culm. This cultivar has the same number of tillers per hill and higher spikelet numbers per panicle compared to Nokyang. 'Mogwoo' has longer leaves compared with other Tongil-type varieties. This new variety is resistant to grain shattering, leaf blast, bacterial leaf blight, and small brown planthopper. The biomass yield of 'Mogwoo' was 1,956 kg/10a in a regional test over three years. The result shows that 'Mogwoo' is adaptable to central and south-east plain areas of Korea.

A New Rice Cultivar 'Manjong', Suitable to the Rice-Cash Crop Double Cropping System (중생 소득작물 후작지 적응 벼 품종 '만종')

  • Lee, Jong-Hee;Park, Dong-Soo;Cho, Jun-Hyeon;Song, You-Chun;Jeon, Myeong-Gi;Kwak, Do-Yeon;Kim, Choon-Song;Yeo, Un-Sang;Lee, Ji-Yoon;Kim, Sang-Yeol;Jung, Kuk-Hyun;Park, No-Bong;Han, Sang-Ik;Yi, Gihwan;Oh, Byeong-Geon;Shin, Mun-Sik;Kang, Jong-Rae;Oh, Sung-Hwan;Hwang, Heung-Goo;Kang, Hang-Won;Ahn, Jin-Gon
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.611-615
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    • 2011
  • 'Manjong' is a new japonica rice cultivar developed in 2009 derived from a cross between 'Yeongdeog34' and 'Nampyeongbyeo' at the Department of Functional Crop, NICS, RDA. This cultivar is suitable for the double cropping system after the cash crop cultivation. Heading date of 'Manjong' is later than 'Keumobyeo' under the late transplanting cultivation on July 10. It has high grain fertility under cold conditions and low premature heading. One of the distinguishing characteristics of this variety is its resistance to major diseases like leaf blast and rice stripe virus disease. However, it shows susceptibility to major insect pests and bacterial blight disease. Milled rice kernels are translucent with non-glutinous endosperm and have 7.2% protein and 18.7% amylose contents. Ripening ratio and head rice ratio is very higher than those of 'Keumobyeo'. The palatability of cooked rice is also better than 'Keumobyeo'. The milled rice yield of 'Manjong' in local adaptability tests after harvest of the cash crop is $4.81MT\;ha^{-1}$. This cultivar is suitable for planting in the plain paddy fields of Honam and Yeonnam regions in Korea.

A New High Yield and Good Quality Rice Cultivar Adaptable to After Economic Crops "Manna" (벼 조생 고품질 소득작물 후작 다수성 "만 나")

  • Ko, Jong Cheol;Nam, Jeong Kwon;Choung, Jin Il;Ha, Ki Yong;Baek, Man Gee;Kim, Ki Young;Sin, Woon Chul;Kim, Woo Jae;Lee, Jae Kil;Ko, Jae Kwon;Kim, Bo Kyeong;Kang, Hyun Jung;Kim, Young Doo;Mo, Young Jun;Kim, Chung Kon
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.529-532
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    • 2008
  • Manna is a new japonica rice cultivar developed from the cross between Iksan438 and Ilmibyeo by the rice breeding team of Department of Rice and Winter Cereal Crop, RDA, in 2005. This cultivar has a short grain shape and about 97 days growth duration from late transplanting to harvesting under Korean climatic conditions. It is resistant to lodging with average 70 cm of culm length. The milled kernels of Manna are translucent with non-glutinous endosperm. It has about 19% amylose content and good palatability of cooked rice compared with Geumobyeo. This cultivar shows resistance to leaf blast and neck blast but susceptibility to bacterial blight and stripe virus diseases. The milled rice yield potential of Manna is about 5.05 MT/ha under the late transplanting cultivation. Manna would be adaptable for the middle and Honam plain of Korea.