• 한국응용곤충학회지
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• 제40권1호
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• pp.44-49
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• 2001

진딧물류 천적 혹파리(Aphidoletes aphidimyza (Rondani))의 온도별 발육 및 복숭아혹진딧물에 대한 포식특성을 실험실 및 온실 조건에서 조사하였다. 온도와 유충의 발육속도와의 상관 을 구하였으며 ((regression; development rate (y) : 0.013348x (temperature)-0.173022, F = 41.14, p<0.01)), 발육영점온도는 $13^{\circ}C$이었다. $\25pm$2$^{\circ}C$ 에서 유충 발육기간은 5.3일 이었고, 1세대기간은 29 일이었다. 복숭아혹진딧물 밀도에 따른 혹파리의 포식량은 arena(직경 9cm)당 복숭아혹진딧물 10마리일 때 4마리를 포식하여 포화상태를 나타냈다. 온실조건에서 진디혹파리의 진딧물 포식은 지체적밀도의존반응과 유사한 반응을 보였다.

• 한국수산과학회지
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• 제47권5호
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• pp.654-658
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• 2014

We report the first identification of a spawning ground of the blackfin flounder Glyptocephlaus stelleri near the Wangdol-cho sea mountains, located in the southern East Sea. Eggs and larval fish of G. stelleri were collected during April and June, 2014, when an abundance of eggs was found in the southern area of Wangdol-cho. Our findings suggest that G. stelleri prefers to spawn in the vicinity of the off-shore sea mountains, where the temperature is between 10 and $12^{\circ}C$ and the water depth is 100 m, rather than inshore.

• 한국양식학회지
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• 제18권3호
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• pp.200-206
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• 2005

We previously pointed out that the polluted sediment elutriate manifestly affected the early events of reproductive outputs in the Pacific oysters, Crassostrea gigas. A serial dilution of priority xenobiotic sediment elutriates determined by gas chromatography/mass spectrometry (GC/MS) were exposed to gametes of the oyster with different stress burdens to detail the maternal stress transfer to its reproductive outputs. There was an apparent critical concentration over which survival and morphogenesis were significantly affected with more profound damage in morphogenesis. The critical concentration which drives mortality and abnormal morphogenesis of the larvae corresponded to a dilution between 10 and 20% of our elutriate. The adverse effects of the early lives by the xenobiotic exposure over the critical concentration were magnified by the maternal stress from the exposed benzo(a)pyrene (BaP), one of the priority polyaromatic hydrocarbons (PAHs) during the maturation condition. These results indicate that maternal transfer of the xenobiotic compounds from oysters living in the contaminated location might represent a significant adverse effect to their larval population of wild seeds.

• 한국패류학회지
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• 제15권2호
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• pp.115-119
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• 1999

We investigated the processes of egg and larval developments for aquaculture technique development of seedling production fo the flat oyster, Ostrea denselamellosa. Teo flat oyster of larviparous type was different from the pacific oyser (ovivarous type) because their larvae (trochophore and prodissoconch larvae) in the gill released into the seawater. The process of egg development was observed by artificial fertilization at $25^{\circ}C$, using a dissecting method. The sizes of Unfertilized eggs ranged from 80 to 90 $\mu\textrm{m}$ and fertilized eggs with globule-shape was 90-100 $\mu\textrm{m}$. The Polar body appeared after fertilization and egg cleavage began within 1 hour, reaching the blastula stage after 10 hours. The trochophore in the gill appeared 2-3 days after fertilization and grew to the prodissoconch larvae (130 140 $\mu\textrm{m}$) having a complete shell after 1-2 days. The shell of prodissoconch larvae grew to 205 220 $\mu\textrm{m}$ after 10 hours, and then they became umbo stage larvae showing oval in shape. The velum of umbo stage larvae was degenerated about 17-20 days after fertilization and grew into a pediveliger with a developed foot, at this time, the shell length size was 320 360 $\mu\textrm{m}$.

• BMB Reports
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• 제44권3호
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• pp.187-192
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• 2011

TGF-$\beta$ activated kinase-1 (TAK1) plays a pivotal role in developmental processes in many species. Previous research has mainly focused on the function of TAK1 in model organisms, and little is known about the function of TAK1 in hymenoptera insects. Here, we isolated and characterized the TAK1 gene from Apis cerana cerana. Promoter analysis of AccTAK1 revealed the presence of transcription factor binding sites related to early development. Real-time quantitative PCR and immunohistochemistry experiments revealed that AccTAK1 was expressed at high levels in fourth instar larvae, primarily in the abdomen, in the intestinal wall cells of the midgut and in the secretory cells of the salivary glands. In addition, AccTAK1 expression in fourth instar larvae could be dramatically induced by treatment with pesticides and organic solvents. These observations suggest that AccTAK1 may be involved in the regulation of early development in the larval salivary gland and midgut.

• International Journal of Industrial Entomology and Biomaterials
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• 제43권2호
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• pp.45-51
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• 2021

High blood cholesterol levels and oxidized cholesterol are risk factors for cardiovascular disease, which displays high annual incidence. Although studies on sericulture products, including pupae, silk protein, and blood lymph, as hypocholesterolemic substances have been reported, insufficient research in this field has been focused on silkworm larvae. Six larval extracts (Low temperature distilled water, LW; hot temperature distilled water, HW; and 30-100% ethanol, E30-E100) were prepared, and their effects on cholesterol metabolism were examined. LW most potently reduced the risk of cholesterol-related disorders. Polyphenols were highly represented in LW, corresponding with its increased antioxidant potency. The cholesterol biosynthesis enzyme, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR) was strongly inhibited by LW. Hepatocytes over-expressed LDL receptor (LDLR) after LW stimulation, promoting cholesterol elimination from plasma. LW also increased ATP binding cassette transporter 1 (ABCA1) gene expression, upregulating HDL biogenesis. In conclusion, LW exhibited strong antioxidant activity, suppressed cholesterol biosynthesis, improved LDL uptake from plasma, and upregulated HDL biosynthesis. In aggregate, these activities could reduce blood cholesterol levels and prevent cardiovascular disease.

• 한국수산과학회지
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• 제55권1호
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• pp.23-29
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• 2022

This study was carried out to clarify the egg, larvae and juveniles development of Misgurnus anguillicaudatus, and relationships of M. anguillicaudatus and M. mizolepis, Cobitididae Fishes. The adult fishes were collected in Samsan-cheon, Haenam-gun, Jeollanam-do, Korea and their spawning inducement was carried by ovaprim injections. The egg shape was circular and the size was average 1.12 mm. The eggs were hatched at 61 to 72 h after fertilization. The newly hatched larvae had an average 3.23 mm in total length (TL). At 5 days after hatching, the larvae reached to post larval stage and they were 10.3 mm in TL. At 19 days after hatching, it reached to juvenile stage and was 25.3 mm in TL. The egg size of M. anguillicaudatus was almost same as M. mizolepis but the hatching period of M. anguillicaudatus has taken longer. It was possible for interspecific distinguishability of M. anguillicaudatus and M. mizolepis when their larvae reached to juvenile stage by the development of keel-like ridges.

• 한국발생생물학회지:발생과생식
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• 제7권1호
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• pp.29-34
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• 2003

이 연구는 자주복의 개체발생 단계에 따른 소화관 외부형태의 변화와 소화관의 발달 양상을 조직학적으로 조사하였다. 부화 직전 수정란의 소화관은 난황낭과 배체의 척추 사이에 위치하였고, 형태는 거의 직선상 이었다. 입은 부화 후 2일째, 항문은 부화 후 4일째 열렸다. 부화 후 4일째 소화관은 식도, 전장, 중장 그리고 직장으로 구분이 되었고, 전장과 중장이 괄약근에 의해 구분되었다. 부화 후 5일째 자어는 전장과 중장 사이가 배측으로 굴곡되었고, 식도상피에 점액세포가 분포하였다. 이 시기에 난황은 대부분 흡수되었다 부화 후 6일째 자어의 소화관은 전장이 두부 쪽으로 팽창하면서 U자형으로 분화하였다. 부화 후 10일 째에 팽창한 전장은 더욱 커져서 구형을 형성하였다. 부화 후 15일째 전장의 배측 부위 중간에서 함입되기 시작하여 전장이 두 부분으로 나누어졌다. 부화 후 21일째 전장의 앞부분이 팽창낭으로 분화하였다. 부화 후 24일째에 후장의 앞부분이 배측으로 다시 회전하였다. 부화 후 30일째 팽창낭은 두부 쪽과 항문 쪽으로 길게 신장되어 어미의 소화관 형태로 발달하였다.

• 한국어병학회지
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• 제22권3호
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• pp.201-210
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• 2009

2007년 3월부터 2008년 2월까지 약 12개월에 걸쳐서 우리나라 동, 서, 남해 연안 지역에서 총 11목 44종 989마리 자연산 해산어류를 대상으로 아니사키스형 유충의 기생상황을 조사하였다. 유충의 감염률은 44종 중 28종에서, 전체 989마리 중 377마리에서 검출되어 평균 38%의 감염률을 보였다. 적출된 유충의 대부분은 형태학적 조사 및 유전학적 조사를 통하여 A. simplex type I 유충으로 동정하였다. 일반적으로 가을 겨울철보다 봄 여름철에 감염률이 높았으며, 같은 어종이라도 크기가 큰 개체에서 감염률이 높게 나타났다. 병리조직학적 검사를 통하여 유충의 기생 부위는 어종에 따라서 다르나 대체로 배설강 주변부를 비롯하여 장간막, 간, 복강지방 등 이였으며, 한두 마리씩 산재해 있는 것과 여러 마리가 함께 모여 있는 것, 피낭에 뭉쳐 있는 것 등 다양하게 나타났다.

• 한국발생생물학회지:발생과생식
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• 제12권3호
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• pp.283-288
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• 2008

이 연구는 2003년 4월부터 5월까지 전라남도 보성군 겸백면 율어리 소재의 보성강 중류에서 투망과 족대를 이용하여 채집된 모래무지 어미들을 전남대학교 해양기술학부 자원생물실험실로 운반하여 실내 사육하면서 난발생 및 자치어 형태발달을 관찰하였다. 모래무지의 수정란은 구형의 침성부착란으로 난경은 $1.98{\pm}0.19mm$(n=50)였고, 반투명하였다. 부화에 소요되는 시간은 수정 후 164시간부터였고, 이 때 근절수가 31$\sim$32개였으며, 배체의 움직임이 활발하였으며, 머리부터 난막을 뚫고 부화하기 시작하였다. 부화 직후의 자어는 전장이 $4.61{\pm}0.83mm$(n=10)로 난황은 아직 흡수하지 않은 상태였으며, 입과 항문은 열려 있지 않았다. 등쪽과 배쪽, 꼬리쪽에 별모양과 점모양의 흑색소포가 산재하였으며, 눈에는 색소포가 진하게 착색되어 있었다. 부화 후 42일째는 전장 $16.22{\pm}0.65mm$(n=10)로 흑색소포가 두부와 등쪽과 체측면에 따라 짙게 산재하였고, 주둥이 양쪽에 수염이 나타났으며, 체형이나 반문이 성어와 완전히 닮아 있어 치어기로 이행하였다.