• Title/Summary/Keyword: large area cell

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The Oogenesis of Three Spot Gourami, Belontiidae, Teleostei (경골어류 등목어과 Three spot gourami의 난자형성과정)

  • Chang, Byung-Soo;Jung, Han-Suk;Joo, Kyung-Bok;Kim, Dong-Heui
    • Applied Microscopy
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    • v.41 no.3
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    • pp.155-161
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    • 2011
  • Three spot gourami (Trichogaster trichopterus Pallas, 1770) is a teleost belonging to Belontiidae. The oogenesis of three spot gourami was investigated by light microscope. The ovary was of light peach color and ellipsoidal shape with the major axis 2 cm and the minor axis 1 cm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocyte, lipid droplets were distributed only in the marginal area first, than at nuclear envelope near. In secondary oocyte, the egg envelope was formed and yolk vesicles was formed in the marginal area. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The fertilized eggs were the colorless, transparent, spherical, adhesive and pelagic type. A large oil droplet was located in vitelline membrane of the fertilized egg. In conclusion, the oogenesis of three spot gourami was characterized by the increase in cell size, the formations of lipid droplets and yolk, the decrease of basophilic substance in the cytoplasm, and formation of one large oil droplets.

Studies on the Physico-Chemical Properties on Several Chitosan Beads (몇 가지 키토산 비드들의 물리화학적 특성에 관한 연구)

  • Ha, Byung-Jo;Lee, Ok-Sub;Lee, Yoon-Sik
    • Applied Chemistry for Engineering
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    • v.7 no.1
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    • pp.186-193
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    • 1996
  • Several chitosan beads were prepared by W/O emulsion and capillary extrusion methods. The surface structures were observed through SEM pictures, the surface areas were determined by BET method, the available amine contents were measured by picric acid titration method, and the results were correlated each other. The beads from W/O emulsion method were smaller in size but had higher hardness, surface area, and available amine contents than the ones from capillary extrusion method. Moreover, the surface area, available amine contents, apparent density and the hardness showed large differences according to the drying processes. As compared between the solvent dehydration and the freeze drying technique in W/O emulsion method, the former showed higher surface area, higher available amine contents and hardness than the latter. SEM pictures revealed that in case of solvent dehydration method, very small pores existed, whereas in case of freeze drying method, cell-like pores existed on the surface. The results of picric acid titration showed that the beads dried by solvent dehydration method were 10 times higher in amine contents than those from freeze drying method, and the amount of amine contents were increased as the surface area was increased. After fluorescamine was reacted with chitosan beads, the fluorescence labeled beads were observed through fluorescence microscope. The results showed that most of primary amine existed on the surface of beads and pores.

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AN ELECTRON MICROSCOPIC STUDY OF THE PERIAPICAL GRANULOMA AND THE PERIAPICAL CYST (치근단육아종(齒根端肉芽腫)과 치근단양종(齒根端襄腫)의 전자현미경적(電子顯微鏡的) 연구(硏究))

  • Yo, In-Ho;Lim, Sung-Sam
    • Restorative Dentistry and Endodontics
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    • v.13 no.2
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    • pp.283-294
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    • 1988
  • The purpose of this study was to investigate the characteristic features of the cells and tissues of the chronic periapical lesions using light microscope and electron microscope. Fifteen dental periapical lesions were obtained from the patients undergoing periapical surgery. Each specimen was divided into two parts along the tooth axis. One part was routinely processed for histopathologic examinations. 12 periapical lesions were diagnosed as granuloma and 3 periapical specimens as periapical cyst. The other part was fixed in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at pH 7.4 and 1% osmic acid in same buffer. They were embedded in Epon 812. The semithin sections were used for the orientation of the lesions and the ultrathin sections were stained conventionally and examined with AEI Corynth 500 electron microscope. The results were as follows. 1. PMN and macrophages, which were dominant cell type, were scattered in small or large numbers throughout the central destructive area of granuloma. In the granulomatous area, plasma cells and lymphoytes were found in significant number and a lot of new capillary formation were revealed. Clefts caused by cholesterol were often seen in the connective tissue. Occasionally foam cells became collected in groups and epithelial proliferation were present. 2. In both granuloma and cyst, some plasma cells contained narrow cisternae of granular endoplasmic reticulum of which was tightly packed with electron dense materials, and other cells exhibited dilated profiles of granular endoplasmic reticulum. 3. In the area where plasma cells and lymphocytes were collected in groups, lymphocytes with well developed nucleolus and profuse cytoplasm were found and differentiating plasma cells were also present. 4. In the epithelial strands of the granulomatous area, epithelial cells contained enlarged endoplasmic reticulum, tonofilaments and ribosoms. Toward the intercellular space epithelial cells protruded a few microvilli. In the intercellular space, exudate-like electron dense materials, most of which was attached to the plasma membrane, appeared. 5. Some foam cells filled with numerous lipid droplets and others had lipid droplets and crystal-like structures. 6. Cyst epithelium consisted of bright cells and dark cells. The former had bright cytoplasm and small amounts of ribosoms, and the latter dark cytoplasm, many ribosoms, mitochondria and elongated microvilli. 7. Epithelial cells near the cyst lumen protruded a lot of long microvilli toward intercellular space and cyst lumen.

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Paralytic Shellfish Toxin Profiles of the Dinoflagellate Alexandrium Species Isolated from Benthic Cysts in Jinhae Bay, Korea (진해만산 와편모조류 Alexandrium속 휴면포자 발아체의 마비성패독 조성)

  • KIM Chang-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.3
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    • pp.364-372
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    • 1995
  • On the outbreak of paralytic shellfish poisoning in April 1993 in most of shellfish harvesting areas in Jinhae Bay, Korea, to clarify the toxin production of causative organism Alexandrium species, 19 axenic clonal isolates established from the benthic resting cysts in three different stations of those culture grounds were subjected to PSP toxin analysis by HPLC. Individual toxin content per cell was highly variable among the strains isolated from a sampling area and originated from an individual cyst. Average toxin contents in those areas revealed higher values of 54-70 fmol/cell. Toxin profiles included C1/C2(epiGTX8/GTX8), GTX1/GTX4 and neoSTX as the major components, and GTX2/GTX3, GTX5, C4, dcSTX and STX as the minor or sporadic ones. neoSTX on the dominant toxins showed not only most diverse compositional changes comprising $5-54 mol\%$ ranges but also no detection on the half of the strains examined, which were implicated in arising of heterogeneity with a genetic trait within a geographical region. When average toxin composition was compared, carbamate toxins comprised large proportions of $57\%,\;54\%\;and\;67\%$ as total toxin in St. 1, St. 2 and St. 4, respectively. These results suggested that an extensive paralytic shellfish toxification in Jinhae Bay could be largely due to the production of highly potent carbamate toxins in the causative dinoflagellate Alexandrium species.

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Feed System Modeling of Railroad using Fuel Cell Power Generation System (연료전지 발전시스템을 이용한 철도급전계통 모델링)

  • Yoon, Yongho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.20 no.4
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    • pp.195-200
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    • 2020
  • With the growing interest in fossil fuel depletion and environmental pollution, railroad cars operating in Korea are in progress as the conversion from diesel to electric vehicles expands. The photovoltaic system, which is applied as an example of the conversion of electric vehicles, is infinite and pollution-free, and can produce energy without generating hazards such as air pollution, noise, heat, and vibration, and maintain fuel transportation and power generation facilities. There is an advantage that is rarely needed. However, the amount of electricity produced depends on the amount of solar radiation by region, and the energy density is low due to the power generation of about 25㎡/ kWp, so a large installation area is required and the installation place has limited problems. In view of these problems, many studies have been applied to fuel cells in the railway field. In particular, the plan to link the fuel cell power generation system railroad power supply system must be linked to the power supply system that supplies power to the railroad, unlike solar and wind power. Therefore, it has a close relationship with railroad cars and the linkage method can vary greatly depending on the system topology. Therefore, in this paper, we study the validity through simulation modeling related to linkage analysis according to system topology.

Weekly irinotecan and carboplatin for patients with small cell lung cancer (소세포 폐암 환자에서 이리노테칸, 카보플라틴 주별 분할 항암요법의 효과)

  • Lee, Hye-Won;Jeong, Eu Gene;Kim, Dong Hyun;Lee, Hyuk;Kang, Bo Hyoung;Um, Soo-Jung;Roh, Meesook;Son, Choonhee
    • Journal of Yeungnam Medical Science
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    • v.31 no.2
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    • pp.82-88
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    • 2014
  • Background: Lung cancer is the most common cause of cancer-related death worldwide and in Korea, and small cell lung cancer (SCLC) is the most deadly tumor type in the different lung cancer histology. Chemotherapy is the main strategy of the treatment for SCLC, and etoposide and platinum regimen has been the only standard chemotherapy for about 30 years. To test feasibility of weekly divided dose irinotecan and carboplatin for Korean patients is the aim of this study. Methods: Patients with histologically or cytologically confirmed extensive stage SCLC were included. Patients with limited stage (LD), who could not tolerate concurrent chemoradiotherapy were also included. All the patients received irinotecan $60mg/m^2$, carboplatin 2 area under the curve at day 1, 8, and 15 every 4 weeks. Study regimen was discontinued when the disease progressed or intolerable side effects occurred. No more than 6 cycles of chemotherapy were given. Results: Total 47 patients were enrolled, among them 9 patients were LD. Overall response rate was 74.5% (complete response, 14.9%; partial response, 59.6%). Side effects greater than grade 3 were neutropenia (25.5%), fatigue (12.8%), thrombocytopenia (8.5%), sepsis (4.3%), and pancytopenia (2.1%). There was no treatment related death. Conclusion: Weekly divided irinotecan and carboplatin regimen is effective, and safe as a first line therapy for both stage of SCLC. Large scaled, controlled study is feasible.

Artificial Sea Ice Increasing to Mitigate Global Warming (지구 온난화 경감을 위한 인공해빙증가)

  • Byun, Hi-Ryong;Park, Chang-Kyun
    • Journal of the Korean earth science society
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    • v.36 no.6
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    • pp.501-511
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    • 2015
  • This study suggests a method of alleviating global warming by the increase of the Earth surface albedo through Artificial Sea ice Increasing (ASI) over the Available Freezing Areas (AFA). The method is developed based on the fact that the large sea surface area in or near the Arctic and the Antarctic has no ice even though both water and air temperatures are below zero and the artificial sea ice generation is thus available. The mean energy of $0.85Wm^{-2}$, which was suspected of adding to the earth by the global warming effect was calculated to offset at once when the sea ice area about $4.09{\times}10^6km^2$ was additionally increased. In addition, three techniques for producing ice plates on the sea surface (using ships, installation apparatus, and floating matter such as Green Cell Foam) for ASI were proposed. According to the result of simple analysis using the energy balance model, when ASI was maximally operated only for 3 months (September, October, and November) over AFA, it is expected that the annual mean temperature of earth surface would be decreased about $0.11^{\circ}C$ in the following year. On the other hand, in case of generating the artificial sea ice in all four seasons, a risk of triggering snowball earth was detected.

Numerical Analysis of Characteristics of Cellular Counterflow Diffusion Flames near Radiative Extinction Limit (복사 열손실에 의한 소염근처에서 셀모양 대향류 확산화염의 특성에 대한 수치해석)

  • Lee, Su Ryong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.38 no.6
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    • pp.493-500
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    • 2014
  • Nonlinear characteristics of cellular counterflow diffusion flame near the radiative extinction limit at large Damk$\ddot{o}$hler number are numerically investigated. Lewis number is assumed to be 0.5 and flame evolution is calculated by imposing an infinitesimal disturbance to a one-dimensional(1-D) steady state flame. The early stage of nonlinear development is very similar to that predicted in a linear stability analysis. The disturbance with the wavenumber of the fastest growing mode emerges and grows gradually. Eventual, an alternating pattern of reacting and quenching stripes is developed. The cellular flame temperature is higher than that of 1-D flame because of the gain of the total enthalpy. As the Damk$\ddot{o}$hler number is further increased, the shape of the cell becomes circular to increase the surface area per unit reacting volume. The cellular flames do not extinguish but survive even above the 1-D steady state extinction condition.

RECONSTRUCTION OF INTRAORAL DEFECT USING RADIAL FOREARM FREE FLAP: A CASE REPORT (유리전완피부피판을 이용한 구강내 결손의 재건례)

  • Lee, Sang-Chull;Kim, Yeo-Gab;Ryu, Dong-Mok;Lee, Baek-Soo;Kwon, Yong-Dae
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.20 no.3
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    • pp.246-249
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    • 1998
  • With the recent progress of microsurgical techniques, radial forearm free flap has an established place in oral reconstruction. Providing thin, soft and pliable skin with a large and constant vascular pedicle, this flap is optimal for intraoral reconstruction. One of the disadvantages of the flap is donor site morbidity, therefore various methods can be used to reduce it. A male complained of palatal and retromolar area mass with ulceration, which was diagnosed as squamous cell carcinoma. He also complained of discomfort and mouth opening limitation, attributed to the location and characteristics of the mass. Because of mouth opening limitation, mandibular swing approach was performed to allow for the surgical approach to the mass. After the surgical excison of the lesion, the intraoral defect was successfully reconstructed with radial forearm free flap.

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Performance Evaluation of a Pilot Interference Cancellation Scheme in a WCDMA Wireless Repeater (WCDMA 무선 중계기에서 파일럿 간섭제거 기법의 성능평가)

  • Kim, Sun-Ho;Shim, Hee-Sung;Im, Sung-Bin
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.46 no.6
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    • pp.111-117
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    • 2009
  • In the wideband code division access (WCDMA) systems, a pilot channel is used to determine WCDMA network coverage, cell identification, synchronization, timing acquisition and tracking, user-set handoff, channel estimation, and so on. A wireless repeater, which is deployed in the urban area for the WCDMA system to meet the growing demand on wireless communication services, has the possibility to receive several pilot signals from a large number of base stations, however, cannot distinguish its service base station's signal among them. This pilot interference results in frequent handoffs in the user equipment, which degrades the radio reception, transmission efficiency, quality of service, and channel capacity and increases the unwanted power consumption. In this paper, thus, we propose a pilot pollution interference cancellation scheme using one of the adaptive estimation algorithms, normalized least mean square (NLMS), which is applicable to a wireless repeater. We carried out link-level and network-level computer simulations to evaluate the performance of the proposed scheme in a wireless repeater. The simulation results verify the bit error rate (BER) improvement in the link level and the call drop probability improvement in the network level.