• Title/Summary/Keyword: lactic acid bacteria

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INCIDENCE OF LACTIC ACID BACTERIA ISOLATED FROM INDIGENOUS DAHI

  • Masud, T.;Sultana, K.;Shah, M.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.4 no.4
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    • pp.329-331
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    • 1991
  • Fifty samples of indigenous dahi were collected randomly from the local market of Rawalpindi/Islamabad to determine the incidence of lactic acid bacteria. The micro-organisms isolated were Lactobacillus bulgaricus (86%), Streptococcus themophilus (80%), Streptococcus lactis (74%), Lactobacillus helveticus (34%), Streptococcus cremoris (30%), Lactobacillus casei (20%) and Lactobacillus acidophilus (14%) respectively. The results of the present study revealed that indigenous dahi contains mixtures of lactic acid bacteria and thus the quality of dahi may vary with the type of starter culture used for inoculation.

Predominant Lactic Acid Bacteria from Salted Sea Food

  • Cho, Gyu-sung;Bae, Chae-Yun;Do, Hyung-Ki;Shin, Hyeun-Kil
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2005.10a
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    • pp.319-323
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    • 2005
  • Lactic acid bacteria are dominant microflora in many kinds of fermented foods. In this study, dominant microflora, especially lactic acid bacteria were isolated from salted sea food, and we determined physiological characteristics, and assayed specific property such as bacteriocin activity. The population of lactic acid bacteria as well as aerobic mesophilic counts was at the level of $10^7$ cfu /g. Total 17 strains of LAB were isolated from salted sea food sample. The phenotypic characteristics of these strains were determined followed by Bergey's Manual. And genotypic and bacteriocin activity were tested by Schillinger and $L{\ddot{u}}cke^{(7)}$.

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Selective Media for Isolation and Enumeration of Lactic Acid Bacteria from Kimchi (김치에서 젖산균의 선발 및 계수를 위한 선택배지)

  • 이명기;박완수;강국희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.754-760
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    • 1996
  • Selective media for isolation and enumeration of lactic acid bacteria from kimchi were compared using 36 strains of lactic acid bacteria from kimchi and dairy products. Among the selective media, KF Streptococcus agar showed the best result for the selection of Enterococcus group and Pediococus group, and M-17 agar with pH indicator for Lactococcus group, the m-LBS agar for Lactobacillus group, and PES-3 agar for Leuconostoc group.

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Differential Transformation of Ginsenosides from Panax ginseng by Lactic Acid Bacteria

  • Chi, Hyun;Lee, Bo-Hyun;You, Hyun-Ju;Park, Myung-Soo;Ji, Geun-Eog
    • Journal of Microbiology and Biotechnology
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    • v.16 no.10
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    • pp.1629-1633
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    • 2006
  • Ginsenosides have been regarded as the principal components responsible for the pharmacological and biological activities of ginseng. The transformation of ginsenosides with live lactic acid bacteria transformed ginsenosides Rb2 and Rc into Rd, but the reactions were slow. When the crude enzymes obtained from several lactic acid bacteria were used for transformation, those from Bifidobacterium sp. Int57 exhibited the most potent transforming activity of ginsenosides to compound K. In comparison, a relatively higher level of Rh2 was produced by the enzymes from Lactobacillus delbrueckii and Leuconostoc mesenteroides. These results suggest that it is feasible to develop a specific bioconversion process to obtain specific ginsenosides using the appropriate combination of ginsenoside substrates and specific microbial enzymes.

Growth of Lactic Acid Bacteria and Quality Characteristics of Baechu Kimchi Prepared with Various Salts and Concentration (소금의 종류와 침지 농도에 따른 배추김치의 젖산균의 생육과 품질 특성)

  • Kim, Da-Mee;Kim, Kyung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.29 no.3
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    • pp.286-297
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    • 2014
  • This study was conducted to evaluate the growth of lactic acid bacteria and quality characteristics of Baechu kimchi prepared with four types of salt (Solar salt, Flower salt, Hanju salt, and Roasted salt) at two different concentrations (10, 15%) during storage. The quality characteristics of kimchi were examined by investigating acid production, growth of lactic acid bacteria, sensory properties, salinity, texture, and color characteristics. Baechu kimchi was fermented for 18 days at $10^{\circ}C$. The pH and total acidity did not change during storage according to type and concentration of salt. Growth of lactic acid bacteria was not affected by type of salt, whereas it was inhibited at 15% salt concentration after 9 days of fermentation. In the quantitative descriptive analysis of sensory properties, kimchi prepared with 10% salt showed significantly higher scores in term of overall acceptability (p<0.05). Meanwhile, there was no significant difference by type of salt. For texture characteristics, hardness of kimchi prepared with 10% salt was significantly higher than that with 20% salt. For color characteristics, L value (brightness) and b values (yellowness) of kimchi prepared with 10% salt increased during fermentation, whereas a value (redness) did not change by type and concentration of salt. The results of this study show that there were no considerable differences in quality characteristics of Baechu kimchi prepared with various types and concentrations of salt. However, Solar salt resulted in more favorable sensory properties and salinity of kimchi than any other types. Further, kimchi prepared with 10% salt showed significantly higher scores in terms of overall acceptability, growth of lactic acid bacteria, salinity, texture, and color characteristics.

Fermentation Characteristic of Fermented Chestnut Puree by Lactic Acid Bacteria as Starter (유산균을 이용한 밤 발효 퓨레의 발효특성)

  • Lee, Jin-Man;Hur, Sang-Sun
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.2
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    • pp.333-342
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    • 2021
  • This study developed a fermented chestnut puree by lactic acid bacteria fermentation using steamed chestnut paste at 95℃ for 90 min and the quality characteristics were investigated. In addition, quality of the characteristics of the fermented chestnut puree during fermentation by lactic acid bacteria were reported. 12 strains of lactic acid bacteria were inoculated to steamed chestnut paste at a concentration of 2%(v/w), respectively, and incubated at 37℃ for 48 hr. Lactobacillus plantarum(KCTC 21004) was the most superior in acid production among 12 strains of lactic acid bacteria to the fermented chestnut puree. The effect of steamed chestnut concentration, inoculum size and fermentation temperature for fermented chestnut puree on physical properties and fermentation characteristics were investigated. As a result there was no significant difference on physiochemical properties but the optimum concentration of the steamed chestnut for puree properties is 50%.

The Roles of Lactic Acid Bacteria for Control of Fungal Growth and Mycotoxins (곰팡이 생육 및 곰팡이 독소 생산의 억제에 있어서의 유산균의 역할)

  • Kim, Jihoo;Lee, Heeseob
    • Journal of Life Science
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    • v.30 no.12
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    • pp.1128-1139
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    • 2020
  • Over recent years, it has become evident that food and agricultural products are easily contaminated by fungi of Aspergillus, Fusarium, and Penicillium due to rapid climate change, which is not only a global food quality concern but also a serious health concern. Owing to consumers' interest in health, resistance to preservatives such as propionic acid and sorbic acid (which have been used in the past) is increasing, so it is necessary to develop a substitute from natural materials. In this review, the role of lactic acid bacteria as a biological method for controlling the growth and toxin production of fungi was examined. According to recent studies, lactic acid bacteria effectively inhibit the growth of fungi through various metabolites such as organic acids with low molecular weight, reuterin, proteinaceous compounds, hydroxy fatty acids, and phenol compounds. Lactic acid bacteria effectively reduced mycotoxin production by fungi via adsorption of mycotoxin with lactic acid bacteria cell surface components, degradation of fungal mycotoxin, and inhibition of mycotoxin production. Lactic acid bacteria could be regarded as a potential anti-fungal and anti-mycotoxigenic material in the prevention of fungal contamination of food and agricultural products because lactic acid bacteria produce various kinds of potent metabolic compounds with anti-fungal activities.

Antioxidant Activity Study of Artemisia argyi H. Extract Fermented with Lactic Acid Bacteria (젖산균으로 발효한 섬애쑥(Artemisia argyi H.) 추출물의 항산화 활성 연구)

  • Ji Hyun Kim;Nan Kyung Kim;Ah Young Lee;Weon Taek Seo;Hyun Young Kim
    • Journal of Korean Medicine for Obesity Research
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    • v.22 no.2
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    • pp.115-124
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    • 2022
  • Objectives: In this study, we investigated physicochemical characteristics and antioxidant activity of Artemisia argyi H. fermented with lactic acid bacteria. Methods: The A. argyi water extract was fermented using lactic acid bacteria isolated from kimchi at 30℃ for 96 h. To evaluate the physicochemical characteristics, we investigated pH, total acidity, viable cells, free sugars, free organic acids, and free amino acids contents during fermentation. In addition, we examined antioxidant activity of fermented Artemisia argyi H. by measurement of 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazinyl (DPPH) and 2,2'-azubi-bus-3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) scavenging activities. Results: During fermentation time, pH of fermented A. argyi was decreased from 4.57 to 3.22, and total acidity was increased from 0.39% to 1.63%. The number of lactic acid bacteria fermented A. argyi was increased from 1.28×107 CFU/ml to 3.75×108 CFU/ml during fermentation time. The free sugars of fermented A. argyi were confirmed glucose and sucrose. In addition, the organic acid content of fermented A. argyi was the highest in oxalic acid and lactic acid. In the composition of free amino acids, content of ornithine increased from 4.4 mg/100 g to 18.8 mg/100 g compared with non-fermented A. argyi. Furthermore, DPPH and ABTS+ radical scavenging activities of fermented A. argyi increased in a dose-dependent manner. Conclusions: In conclusion, our data suggest that lactic acid fermentation of A. argyi could be used as a functional food for antioxidants.

Effect of addition of lactic acid bacteria on quality of rye silage harvested at early heading stage

  • Kuppusamy, Palaniselvam;Choi, Ki-Choon;Srigopalram, Srisesharam;Ilavenil, Soundharrajan;Park, Hyung-Su;Jung, Jeong Sung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.285-285
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    • 2017
  • This study is aimed to analyze the effect of lactic acid bacteria inoculants (LAB) on quality of rye silage fermentation, harvested at early heading stage. The nutritive values were similar between the control and LAB inoculated silages. The pH of rye silage in LAB inoculation significantly decreased as compared to control (p<0.05). In addition, the content of lactic acid in LAB inoculation significantly increased (p<0.05), but the content of acetic acid in LAB treatments decreased. In addition, lactic acid bacterial counts in LAB inoculation significantly increased as compared to control (p<0.05). Therefore, we suggest that rye silage could be improved by novel lactic acid inoculation.

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Improvement of Orchardgrass (Dactylis glomerata L.) Silage Quality by Lactic Acid Bacteria

  • Ilavenil, Soundharrajan;Muthusamy, Karnan;Jung, Jeong Sung;Lee, Bae Hun;Park, Hyung Soo;Choi, Ki Choon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.4
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    • pp.302-307
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    • 2021
  • In the current study, lactic lactic acid bacteria (LAB) Lactobacillus plantarum and Pediococcus pentosaceus were used as a mixed additive for the production of Orchardgrass silage by ensiled method and nutritional change fermentation ability and microbial content of experimental silages. The addition of LAB to Orchardgrass during ensiling process rapidly reduced the pH of the silages than the non-inoculated silages. In addition, the lactic and acetic acid content of silage was increased by LAB strains than the non-inoculated silages whereas butyric acid content was reduced in silage treated with LAB. A microbiological study revealed that higher LAB but lower yeast counts were observed in inoculated silages compared to non-inoculated silage. Overall data suggested that the addition of LAB stains could have ability to induce the fermentation process and improve the silage quality via increasing lactic acid and decreasing undesirable microbes.