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Differential Transformation of Ginsenosides from Panax ginseng by Lactic Acid Bacteria  

Chi, Hyun (Department of Food and Nutrition, Seoul National University)
Lee, Bo-Hyun (Department of Food and Nutrition, Seoul National University)
You, Hyun-Ju (Department of Food and Nutrition, Seoul National University)
Park, Myung-Soo (Department of Hotel Culinary Art, Anyang Technical College)
Ji, Geun-Eog (Department of Food and Nutrition, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.10, 2006 , pp. 1629-1633 More about this Journal
Abstract
Ginsenosides have been regarded as the principal components responsible for the pharmacological and biological activities of ginseng. The transformation of ginsenosides with live lactic acid bacteria transformed ginsenosides Rb2 and Rc into Rd, but the reactions were slow. When the crude enzymes obtained from several lactic acid bacteria were used for transformation, those from Bifidobacterium sp. Int57 exhibited the most potent transforming activity of ginsenosides to compound K. In comparison, a relatively higher level of Rh2 was produced by the enzymes from Lactobacillus delbrueckii and Leuconostoc mesenteroides. These results suggest that it is feasible to develop a specific bioconversion process to obtain specific ginsenosides using the appropriate combination of ginsenoside substrates and specific microbial enzymes.
Keywords
Ginsenosides; transformation; lactic acid bacteria;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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1 Akao, T., M. Kanaoka, and K. Kobashi. 1998. Appearance of compound K, a major metabolite of ginsenoside Rb1 by intestinal bacteria, in rat plasma after oral administration---Measurement of compound K by enzyme immunoassay. Biol. Pharm. Bull. 21: 245-249   DOI
2 Bae, E. A., M. J. Han, M. K. Choo, S. Y. Park, and D. H. Kim. 2002. Metabolism of 20(S)- and 20(R)-ginsenoside Rg3 by human intestinal bacteria and its relation to in vitro biological activities. Biol. Pharm. Bull. 25: 58-63   DOI   ScienceOn
3 Choi, E. K. and G. E. Ji. 2005. Food microorganisms that effectively hydrolyze O-glycoside but not C-glycoside isoflavones in Puerariae radix. J. Food Sci. 70: C25-C28   DOI   ScienceOn
4 Kim, H. Y., J. H. Yang, and G. E. Ji. 2005. Effect of bifidobacteria on production of allergy-related cytokines from mouse spleen cells. J. Microbiol. Biotechnol. 15: 265-268   과학기술학회마을   DOI   ScienceOn
5 Lee, D. S., Y. S. Kim, C. N. Ko, K. H. Cho, H. S. Bae, K. S. Lee, J. J. Kim, E. K. Park, and D. H. Kim. 2002. Fecal metabolic activities of herbal components to bioactive compounds. Arch. Pharm. Res. 25: 165-169   DOI   ScienceOn
6 Han, B. H., M. H. Park, Y. N. Han, L. K. Woo, U. Sankawa, S. Yahara, and O. Tanaka. 1982. Degradation of ginseng saponins under mild acidic conditions. Planta Med. 44: 146-149   DOI   ScienceOn
7 Karikura, M., T. Miyase, H. Tanizawa, T. Taniyama, and Y. Takino. 1991. Studies on absorption, distribution, excretion and metabolism of ginseng saponins. VII. Comparison of the decomposition modes of ginsenoside-Rb1 and -Rb2 in the digestive tract of rats. Chem. Pharm. Bull. 39: 2357-2361   DOI   ScienceOn
8 Wu, J. Y., B. H. Gardner, C. I. Murphy, J. R. Seals, C. R. Kensil, J. Recchia, G. A. Beltz, G. W. Newman, and M. J. Newman. 1992. Saponin adjuvant enhancement of antigen-specific immune responses to an experimental HIV-1 vaccine. J. Immunol. 148: 1519-1525
9 Huang, K. C. 1999. The Pharmacology of Chinese Herbs. CRC Press, Florida
10 Mochizuki, M., C. Y. Yoo, K. Matsuzawa, K. Sato, I. Saiki, S. Tono-oda, K. Samukiwa, and I. Azuma. 1995. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside Rb2, 20(R)- and 20(S)-ginsenoside Rg3, of Red ginseng. Biol. Pharm. Bull. 18: 1197-1202   DOI
11 Karikura, M., H. Kato, F. Shimada, and S. Yano. 1992. Studies on the enzyme immunoassay of bioactive constituents contained in oriental medicinal drugs. VI. Enzyme immunoassay of ginsenoside Rb1 from Panax ginseng. Chem. Pharm. Bull. 40: 314-317   DOI   ScienceOn
12 Lee, F. C. 1992. Facts About Ginseng, the Elixir of Life. Hollyn International, New Jersey
13 Akao, T., H. Kida, M. Kanaoka, M. Hattori, and K. Kobashi. 1998. Intestinal bacterial hydrolysis is required for the appearance of compound K in rat plasma after oral administration of ginsenoside Rb1 from Panax ginseng. J. Pharm. Pharmacol. 50: 1155-1160   DOI   ScienceOn
14 Tawab, M. A., U. Bahr, M. Karas, M. Wurglics, and M. Schubert-Zsilavecz. 2003. Degradation of ginsenosides in humans after oral administration. Drug Metab. Dispos. 31: 1065-1071   DOI   ScienceOn
15 Sato, K., M. Mochizuki, I. Saiki, Y. C. Yoo, K. Samukawa, and I. Azuma. 1994. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng-ginsenoside Rb2. metastasis by a saponin of Panax ginseng-ginsenoside Rb2. Biol. Pharm. Bull. 17:635-639   DOI   ScienceOn
16 Hasegawa, H., J. W. Sung, and Y. Benno. 1997. Role of human intestinal Prevotela oris in hydrolyzing ginseng saponins. Planta Med. 63: 436-440   DOI   ScienceOn
17 Park, S. Y., G. E. Ji, Y. T. Ko, H. K. Jung, Z. Ustunol, and J. J. Pestka. 1999. Potentiation of hydrogen peroxide, nitric oxide, and cytokine production in RAW 264.7 macrophage cells exposed to human and commercial isolates of Bifidobacterium. Int. J. Food Microbiol. 46: 231-241   DOI   ScienceOn
18 Chang, H. M. 1986. Pharmacology and Application of Chinese Material Medica. Vol 1. World Scientific, Singapore
19 Bae, E. A., S. Y. Park, and D. H. Kim. 2000. Constitutive beta-glucosidases hydrolyzing ginsenoside Rb1 and Rb2 from human intestinal bacteria. Biol. Pharm. Bull. 23: 1481-1485   DOI   ScienceOn
20 Kanaoda, M., T. Akao, and K. Kobashi. 1994. Metabolism of ginseng saponins, ginsenosides, by human intestinal bacteria. J. Trad. Med. 11: 241-245
21 Hasegawa, H., J. H. Sung, S. Matsumiya, and M. Uchiyama. 1996. Main ginseng saponin metabolites formed by intestinal bacteria. Planta Med. 62: 453-457   DOI   ScienceOn
22 Lee, B. H. and G. E. Ji. 2005. Effect of Bifidobacterium cell fractions on IL-6 production in RAW 264.7 macrophage cells. J. Microbiol. Biotechnol. 15: 740-744   과학기술학회마을
23 Ko, S. R., K. J. Choi, K. Uchida, and Y. Suzuki. 2003. Enzymatic preparation of ginsenosides Rg2, Rh1, and F1 from protopanaxatriol-type ginseng saponin mixture. Planta Med. 69: 285-286   DOI   ScienceOn
24 Lee, B. H., H. J. You, M. S. Park, B. Kwon, and G. E. Ji. 2006. Transformation of the glycosides from food materials by probiotics and food microorganisms. J. Microbiol. Biotechnol. 16: 497-504   과학기술학회마을