Studies on pathogenicities and developmental stages of Nosema apis (Zander, 1909) were carried out through artificial infection to Nosema free honey bees with various levels of spores isolated from local honey bee colony. The results obtained were summarized as follows: 1. The clinical symptoms were observed as dysentery, enteritis of mid-gut (enlargement and decoloration), crawling posture and shortening of the longevity of worker bees in the rearing honey bee colony inoculated with the spores. 2. Number of spores harvested from laboratory rearing honey bees were progresively increased to 4 weeks after inoculation. The regression equations and coefficients of correlations to various spore levels were as follows in each treatment colony. Colony 1. ($$1,000{\times}10^4spores/ml$$) $$y_{c1}=471{\times}10^{4}x+454{\times}10^4(r=0.65^*$$) Colony 2. ($$500{\times}10^4spores/ml$$) $$y_{c2}=340{\times}10^{4}x+207.8{\times}10^4(r=0.99^{**}$$) Colony 3. ($$100{\times}10^4spores/ml$$) $$y_{c3}=150{\times}10^{4}x+84.2{\times}10^4(r=0.99^{**}$$) Colony 4. ($$10{\times}10^4spores/ml$$) $$y_{c4}=13.8{\times}10^{4}x+13{\times}10^4(r=0.98^{**}$$) 3. Average longevity of worker bees artificially infected with Nosema apis was shortened as 21.7~43.8% compare to the control. (p<.05, p<.01) 4. The spores which were isolated from honey bee colony infected with Nosema disease were ovoid or spherical form, and measured, as a rule, from $4.7{\mu}m$ to $6.1{\mu}m$ (mean $5.3{\mu}m$) in length and from $2.4{\mu}m$ to $3.2{\mu}m$ (mean $2.9{\mu}m$) in width. 5. In the mid-gut of honey bees, the spore was progresively germinated and became trophozoite stage. The trophozoites were grown to meronts and their binary fission were begun. The divided two sporoblasts were developed to the spores which had elastic membrane. The new spores were shed in excreta of honey bees 10~15 day after inoculation at $25{\pm}2$ centigrade. 6. The ultrastructure of spore membrane consisted of three layers, such as, outer, middle and inner layer. The sporoplasm consisting lamellar structure occupied only anterior part of the spore and was often extended to posterior direction where definite vacuoles and a polar filament was able to detect.
This study was aimed for evaluating the validity of relative-productivity index on the basis of working hour(RPHW index) designed by author as new productivity index and drawing up a plan of bettering productivity to cope with financial difficulty of dental laboratories. Fortyeight dental laboratories extracted by cluster-sampling method form all the dental laboratories in Seoul were subjected for this study. And in each of them, the term of establishment which was divied into three group as short-term, mid-term and long-term group, the scale of dental laboratory which as divided into two group by number of dental technician as small-scale group and large-scale group, the salary system which was composed of salary criteria, pay according to ablility criteria and collectiveagree, the salary level, simple labor productivity index(SLP index), relative-productivity index on the basis of worker number(ROWN) and relative-productivity index on the basis of working hour(RPWH index) were surveyed through the self-administractive questionaires. The results as follows : Most of dental laboratories(93.6%) were managed by non-professional managers. The establishment rate per year of dental laboratory was the increase. The mean number of employees of dental laboratories was $7.00{\pm}3.90$ person. The ratio of smallscale group(under the 5 employees) was 42.6% and as the term of establishment was shorter, the ratio of small-scale group was higher. The mean establishment area of dental laboratories was $24.49{\pm}10.97$ unit and the mean establishment area per head of dental laboratories was $4.05{\pm}3.90$ unit. The estabilshment area and area per head were not significantly associted with the term of establishment, but as the term of establishment was shorter, the estabkishment area per head was slightly wider. The establishment area per head in small-scale group was significantly wider than large-scale group(over the 6 emplayees) The salary criteria(54.4%), pay according to ability(79.2)m ability criteria(77.1%) and collectiveagree(79.2%) as salary systems were used in the most of all dnetal laboratoies. The all salary systems were not significantly associated with the term of establishment and the scale of dental laboratories. The monthly mean salary level of dental laboratoies was $125.64{\pm}31.06$ milion won. The monthly salary level was not significantly associated with the term of establishment and the scale of dental laboratores. But the monthly salary level in the short-term group and the small-scale group were slightly lower than others. The SLP index, the RPWN index and the RPWH index of dental laboratories were $132.16{\pm}48.41$, $382.41{\pm}128.76$ and $76.06{\pm}25.11$, respectively. The SLP, the RPWN and the RPWH of dental laboratories were not significantly associated with the term SLP, the RPWN and the RPWH of dental laboratories were significantly associated with the area of dental laboratory and the salary level. Except for only the association salary criteria among salary system with RPWH of dental laboratories, all other salary system were not associated with the SLP, the RPWN and the RPWH of dental laboratories.
Journal of Korean Society of Occupational and Environmental Hygiene
/
v.29
no.3
/
pp.289-297
/
2019
Objectives: To identify the positive rate of and the risk factors associated with latent tuberculosis infection(LTBI) in mine workers, the objectives of the present study evaluated those among former mine workers. Methods: Between January 2015 and May 2017, former male mine workers who had been subjects for epidemiology research for work-related chronic obstructive pulmonary disease(COPD) and had received QuantiFERON-$TB^{(R)}$ Gold In-Tube(QFT-GIT) from the Institute of Occupation and Environment(IOE) under Korea Workers' Compensation and Welfare Service(KCOMWEL) were selected as the study subjects. To identify significant variables for increased risk of LTBI, logistic regression analysis was performed. Results: A total of 736 male former mine workers were selected as study subjects. The positive rate of LTBI among subjects was 69.2%(509/736). The current smoking[odds ratio(OR), 2.3; 95% confidence interval(CI), 1.1-4.9], COPD(OR, 1.4; 95% CI, 0.9-2.3), department loading(OR, 1.8; 95% CI, 0.9-3.4) and mining(OR, 1.5; 95% CI, 0.9-2.5), and working duration of over 20(OR, 1.6; 95% CI, 0.9-3.1) and over 30 years(OR, 2.2; 95% CI, 0.9-4.9) were associated with increased risk of LTBI. The interferon-gamma(IFN-${\gamma}$) level after stimulation with Mycobacterium tuberculosis(MTB)-specific antigens showed a significantly negative correlation with age(r=-0.126). Conclusions: The present study determined that the high positive rate of LTBI among mine workers was associated with not only the host factors but also the occupational exposure to mine dust.
Journal of Korean Society of Occupational and Environmental Hygiene
/
v.30
no.1
/
pp.67-74
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2020
Objectives: Previous study has shown that the positive rate of latent tuberculosis infection(LTBI) among former workers in dusty environments was higher than that among high-risk groups of tuberculosis(TB). The objective of the present study was to identify the development of active TB among former workers in dusty environments diagnosed with LTBI. Methods: Between January 2015 and May 2017, 796 former workers in dusty environments who had been subjects of epidemiology research for work-related chronic obstructive pulmonary disease(COPD) had received the QuantiFERON-TB® Gold In-Tube(QFT-GIT) from the Institute of Occupation and Environment(IOE) under the Korea Workers' Compensation and Welfare Service(KCOMWEL). Among them, 437 participants who received a health examination for work-related pneumoconiosis between January 2015 and December 2018 were selected as study subjects. Active TB was defined as a positive result for active PTB and non-tuberculosis mycobacteria infection in the result of the Pneumoconiosis Examination Council's assessment by KCOMWEL. Results: A total of 437 subjects were followed up for 2.1 years. Four of them(4/437, 0.9%) developed active TB during the follow-up period. The attack rate of active TB among subjects who were diagnosed LTBI positive and those who were diagnosed LTBI negative were 0.9%(3/320) and 0.9%(1/115), respectively. Conclusions: Most previous studies reported that the attack rate of the development of active TB in subjects who had been diagnosed LTBI positive was higher than that among subjects who had been diagnosed LTBI negative. To the contrary, the present study found that the rate of developing active TB among former workers in dusty environments diagnosed as LTBI positive was not higher than that in those who were diagnosed LTBI negative.
In this study, it was evaluated the fire risk during welding cutting tasks. Welding-cutting machines are representatively used at construction sites. Inverter AC/DC TIG welding macnine and inverter air plasma cutting machine were used in experiments. Temperature of spreaded cinders was measured using a thermal camera. Cinder sizes and spread range were measured according to the height and input current. It was also evaluated the fire risk during welding-cutting process, when flammable materials were located around the working area. There were used hay, dust fence, urethane foam, vinyl, paper and oil as flammable materials. Temperature of spreaded cinders was reached at about $450^{\circ}C$. Cinders were spread approximately 4.7 m, when a worker carried out cutting process at 2.5 m height. The possibility of a fire is very high, when flammable materials were located around the working area.
Journal of Korean Society of Occupational and Environmental Hygiene
/
v.29
no.4
/
pp.582-589
/
2019
Objectives: Although active pulmonary tuberculosis(active PTB) is manifested as one of the complications of pneumoconiosis, attacks of active PTB among patients with pneumoconiosis is still unexplored. The objective of the present study was to identify the attack rate of active PTB among workers in dusty environments who were diagnosed with pneumoconiosis. Methods: The study was performed using the results of the Pneumoconiosis Examination Council's assessment from the Korea Workers' Compensation and Welfare Service(KCOMWEL) database between January 1, 1984 and December 31, 2017. Pneumoconiosis was defined as Category 1 or more in the radiological findings of pneumoconiosis. Active PTB was defined as a positive result for active PTB in the results of the Pneumoconiosis Examination Council's assessment. Results: A total of 37,946 workers in dusty environments who received a health examination for diagnosing pneumoconiosis between January 1, 1984 and December 31, 2017 were selected as study subjects. The attack rate of active TB among subjects who were diagnosed with pneumoconiosis and those who were diagnosed without pneumoconiosis were 8.5% and 1.4%, respectively. In the multivariate logistic analysis including age, sex, radiological findings, complications, male[odds ratio(OR), 2.0; 95% confidence interval(CI), 1.4-3.1] and pneumoconiosis(OR, 6.5; 95% CI, 5.7-7.4) were associated with an increased risk of developing active PTB. Conclusions: The present study determined that dusty workers who were diagnosed with pneumoconiosis had a high rate of active PTB compared to TB patients and patients who were diagnosed with silicosis. Therefore, in addition to silicosis, it is necessary to include pneumoconiosis among the high-risk groups for TB.
Zalk, David M.;Spee, Ton;Gillen, Matt;Lentz, Thomas J.;Garrod, Andrew;Evans, Paul;Swuste, Paul
Safety and Health at Work
/
v.2
no.2
/
pp.105-121
/
2011
Objectives: This paper presents the framework and protocol design for a construction industry risk management toolbox. The construction industry needs a comprehensive, systematic approach to assess and control occupational risks. These risks span several professional health and safety disciplines, emphasized by multiple international occupational research agenda projects including: falls, electrocution, noise, silica, welding fumes, and musculoskeletal disorders. Yet, the International Social Security Association says, "whereas progress has been made in safety and health, the construction industry is still a high risk sector." Methods: Small- and medium-sized enterprises (SMEs) employ about 80% of the world's construction workers. In recent years a strategy for qualitative occupational risk management, known as Control Banding (CB) has gained international attention as a simplified approach for reducing work-related risks. CB groups hazards into stratified risk 'bands', identifying commensurate controls to reduce the level of risk and promote worker health and safety. We review these qualitative solutions-based approaches and identify strengths and weaknesses toward designing a simplified CB 'toolbox' approach for use by SMEs in construction trades. Results: This toolbox design proposal includes international input on multidisciplinary approaches for performing a qualitative risk assessment determining a risk 'band' for a given project. Risk bands are used to identify the appropriate level of training to oversee construction work, leading to commensurate and appropriate control methods to perform the work safely. Conclusion: The Construction Toolbox presents a review-generated format to harness multiple solutions-based national programs and publications for controlling construction-related risks with simplified approaches across the occupational safety, health and hygiene professions.
Nowadays, Genomic data constitutes one of the fastest growing datasets in the world. As of 2025, it is supposed to become the fourth largest source of Big Data, and thus mandating adequate high-performance computing (HPC) platform for processing. With the latest unprecedented and unpredictable mutations in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the research community is in crucial need for ICT tools to process SARS-CoV-2 RNA data, e.g., by classifying it (i.e., clustering) and thus assisting in tracking virus mutations and predict future ones. In this paper, we are presenting an HPC-based SARS-CoV-2 RNAs clustering tool. We are adopting a data science approach, from data collection, through analysis, to visualization. In the analysis step, we present how our clustering approach leverages on HPC and the longest common subsequence (LCS) algorithm. The approach uses the Hadoop MapReduce programming paradigm and adapts the LCS algorithm in order to efficiently compute the length of the LCS for each pair of SARS-CoV-2 RNA sequences. The latter are extracted from the U.S. National Center for Biotechnology Information (NCBI) Virus repository. The computed LCS lengths are used to measure the dissimilarities between RNA sequences in order to work out existing clusters. In addition to that, we present a comparative study of the LCS algorithm performance based on variable workloads and different numbers of Hadoop worker nodes.
Fitriana, Yuyun;Tampubolon, Desi Apriani Teresa;Suharjo, Radix;Lestari, Puji;Swibawa, I Gede
The Plant Pathology Journal
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v.38
no.5
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pp.449-460
/
2022
This study was performed to reveal phenotypic characters and identity of symbiont bacteria of Nasutitermes as well as investigate their potential as antagonist of plant pathogenic fungi. Isolation of the symbiont bacteria was carried out from inside the heads and the bodies of soldier and worker termite which were collected from 3 locations of nests. Identification was performed using phenotypic test and sequence of 16S ribosomal DNA (16S rDNA). Antagonistic capability was investigated in the laboratory against 3 phytopathogenic fungi i.e., Phytophthora capsici, Ganoderma boninense, and Rigidoporus microporus. Totally, 39 bacterial isolates were obtained from inside the heads and the bodies of Nasutitermes. All the isolates showed capability to inhibit growth of P. capsici, however, 34 isolates showed capability to inhibit growth of G. boninense and 32 isolates showed capability to inhibit growth of R. microporus. Two bacterial strains (IK3.1P and 1B1.2P) which showed the highest percentage of inhibition were further identified based on their sequence of 16S rDNA. The result showed that 1K3.1P strain was placed in the group of type strain and reference strains of Lysinibacillus fusiformis meanwhile 1B1.2P strain was grouped within type strain and reference strains Paenibacillus alvei. The result of this study supply valuable information on the role of symbiont bacteria of Nasutitermes, which may support the development of the control method of the three above-mentioned phytopathogenic fungi.
Purpose: This study aimed to find overseas workplace and improve global competence through the preference survey on overseas employment by dental technology students. Methods: The survey sample consisted of 250 randomly selected dental technology students. Survey was conducted from March 1 to May 1 in 2012. Total of 245(98.0%) replies and analyzed 236 questionnaires excluding 9 incomplete questionnaires. The questionnaires used in this study consisted of 7 items for general information, overseas employment characteristics of 10 items, 7 items for overseas employment activation plane and job competency development of 7 items. Collected data were analyzed using SPSS(Statistical Package for Social Sciences) Win 19.0 statistics program. Results: Regarding general characteristics of the subjects, there were 131 third graders(55.5%), 63 first graders(26.7%) and 42 second graders(17.8%) among 130 males(55.1%) and 106 females(44.9%). 221(93.6%) of the subjects had no experience in language training. Students who had clinical training for 1-5 months were 123(52.1%), and 24(10.2%) students had more than six months. 89(37.7%) of the subjects had no clinical training. 155(65.7%) of the subjects hope to work with korean owner, and 81(34.3%) chose foreign owner. Favored working countries were Australia(41.5%), the United States(29.2%), Canada(18.2%) and other(11.0%). The field of dental ceramic was indicated to be the highest proportion of 104(44.1%). Period of training were 3 hours(40.3%) and 6 hours(35.2%). The most important training were language-centered education(54.2%), Job-oriented education(24.2%), local culture education(16.1%), other(3.0%) and Leadership Training(2.5%). The subjects chose overseas worker(44.9%), working-level practitioner (28.8%), successfully employed dental technology graduate(19.5%a) and professor(3.4%) as an instructor. The subjects get education and training information from professor(40.3%), other(28.0%), senior(14.4%), job site(8.9%) and acquaintance(8.5%). A credit exchange(2.46 points), a joint degree program(2.46 points), and a foreign professor(2.33 points) were needed to activate the overseas employment. A kind of dental prosthesis(3.58 points), carving tooth morphology(3.38 points), and majors of dental technology(3.30 points) were indicated to develop job competency for overseas employment. Age, year, clinical training experience and company owner were statistically meaningful data among the general characteristics affecting job competency development. Conclusion: The college needs to offer variety programs such as foreign language-centered education and a local job competency development program to graduates to be connected with international workplace and employment.
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