• 제목/요약/키워드: laboratory method

검색결과 7,999건 처리시간 0.038초

Capabilities of stochastic response surface method and response surface method in reliability analysis

  • Jiang, Shui-Hua;Li, Dian-Qing;Zhou, Chuang-Bing;Zhang, Li-Min
    • Structural Engineering and Mechanics
    • /
    • 제49권1호
    • /
    • pp.111-128
    • /
    • 2014
  • The stochastic response surface method (SRSM) and the response surface method (RSM) are often used for structural reliability analysis, especially for reliability problems with implicit performance functions. This paper aims to compare these two methods in terms of fitting the performance function, accuracy and efficiency in estimating probability of failure as well as statistical moments of system output response. The computational procedures of two response surface methods are briefly introduced first. Then their capabilities are demonstrated and compared in detail through two examples. The results indicate that the probability of failure mainly reflects the accuracy of the response surface function (RSF) fitting the performance function in the vicinity of the design point, while the statistical moments of system output response reflect the accuracy of the RSF fitting the performance function in the entire space. In addition, the performance function can be well fitted by the SRSM with an optimal order polynomial chaos expansion both in the entire physical and in the independent standard normal spaces. However, it can be only well fitted by the RSM in the vicinity of the design point. For reliability problems involving random variables with approximate normal distributions, such as normal, lognormal, and Gumbel Max distributions, both the probability of failure and statistical moments of system output response can be accurately estimated by the SRSM, whereas the RSM can only produce the probability of failure with a reasonable accuracy.

Feasibility of Coculture Method for Production of Chimeric Mice Using J1 Embryonic Stem Cells

  • Shin Hye-Jun;Park Sung-Sik;Kim Sun-Uk;Cho Sang-Mi;Han Ying-Hao;Kim Hyun-Sun;Kim Sang-Geun;Lee Dong-Seok;Yu Dae-Yeul
    • 대한의생명과학회지
    • /
    • 제10권4호
    • /
    • pp.391-395
    • /
    • 2004
  • The demand for the production of gene-defective mice from embryonic stem (ES) cells is increasing to clarify decisive gene function in vivo. Although blastocyst injection is widely used to generate ES cell-mediated knockout mice, coculture method has been alternatively used because of several advantages, such as low cost and simple procedure. Thus, this experiment was designed to demonstrate the feasibility of the coculture method using J1 ES cells, which are known to be efficient for blastocyst injection. Eight-cell embryos were harvested from 2.5 days post-coitum (dpc), denuded with acid tyrode's solution, and transferred onto trypsinized J1 ES cells. Aggregation was carried out following two typical methods, which are simple coculture method and aggregation in groove prepared by aggregation needle. Successfully aggregated-embryos were developed to blastocysts for 24 h and transferred into uterus of pseudo-pregnant foster mother. Chimeric offspring was judged by coat pigmentation. In this study, we could obtain chimeric mice from all the two aggregation methods, but the chimera production efficiencies in coculture using groove were three times higher at least than those in the other group. In conclusion, these observations suggest that coculture method should be available for production of knockout mice from J1 ES cells. Presently, the germ-line transmission rates of the chimeras produced from the two methods are under investigation.

  • PDF

Direct Colorimetric Assay of Microcystin Using Protein Phosphatase

  • Oh, Hee-Mock;Lee, Seog-June;Kim, Jee-Hwan;Park, Chan-Sun;Yoon, Byung-Dae
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제5권6호
    • /
    • pp.418-421
    • /
    • 2000
  • A new direct colorimetric assay of microcystin in water and algal samples is proposed consisting of two procedures as follows: 1) the elimination of phosphorus in the sample and concentration of microcystin using a C(sub)18 cartridge, 2) the detection of the released phosphorus by the ascorbic acid method and determination of protein phosphatase (PP) inhibition by microcystin. The optimum amounts of phosphorylase ${\alpha}$ and PP-1 in 50 ${\mu}$L concentrated sample were 50$\mu\textrm{g}$/50${\mu}$L buffer and 1.0unit/50${\mu}$L buffer, respectively, for the best assay. The pH for the maximum activity of PP-1 was 8. The minimum detectable concentration for this method was about 0.02$\mu\textrm{g}$/L, which is sufficient to meet the proposed guideline level of 1$\mu\textrm{g}$ microcystin/L in drinking water. Consequently, it would seem that the proposed direct colorimetric assay using PP is a rapid, easy, and convenient method for the detection of microcystin in water and algal samples.

  • PDF

Application of SA-SVM Incremental Algorithm in GIS PD Pattern Recognition

  • Tang, Ju;Zhuo, Ran;Wang, DiBo;Wu, JianRong;Zhang, XiaoXing
    • Journal of Electrical Engineering and Technology
    • /
    • 제11권1호
    • /
    • pp.192-199
    • /
    • 2016
  • With changes in insulated defects, the environment, and so on, new partial discharge (PD) data are highly different from the original samples. It leads to a decrease in on-line recognition rate. The UHF signal and pulse current signal of four kinds of typical artificial defect models in gas insulated switchgear (GIS) are obtained simultaneously by experiment. The relationship map of ultra-high frequency (UHF) cumulative energy and its corresponding apparent discharge of four kinds of typical artificial defect models are plotted. UHF cumulative energy and its corresponding apparent discharge are used as inputs. The support vector machine (SVM) incremental method is constructed. Examples show that the PD SVM incremental method based on simulated annealing (SA) effectively speeds up the data update rate and improves the adaptability of the classifier compared with the original method, in that the total sample is constituted by the old and new data. The PD SVM incremental method is a better pattern recognition technology for PD on-line monitoring.

Thruster fault diagnosis method based on Gaussian particle filter for autonomous underwater vehicles

  • Sun, Yu-shan;Ran, Xiang-rui;Li, Yue-ming;Zhang, Guo-cheng;Zhang, Ying-hao
    • International Journal of Naval Architecture and Ocean Engineering
    • /
    • 제8권3호
    • /
    • pp.243-251
    • /
    • 2016
  • Autonomous Underwater Vehicles (AUVs) generally work in complex marine environments. Any fault in AUVs may cause significant losses. Thus, system reliability and automatic fault diagnosis are important. To address the actuator failure of AUVs, a fault diagnosis method based on the Gaussian particle filter is proposed in this study. Six free-space motion equation mathematical models are established in accordance with the actuator configuration of AUVs. The value of the control (moment) loss parameter is adopted on the basis of these models to represent underwater vehicle malfunction, and an actuator failure model is established. An improved Gaussian particle filtering algorithm is proposed and is used to estimate the AUV failure model and motion state. Bayes algorithm is employed to perform robot fault detection. The sliding window method is adopted for fault magnitude estimation. The feasibility and validity of the proposed method are verified through simulation experiments and experimental data.

Degrees of Freedom of 3-user MIMO Interference Channels with Instantaneous Relay Using Interference Alignment

  • Qiang, Wang;Yuquan, Shu;Minhua, Dong;Ji, Xu;Xiaofeng, Tao
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • 제9권5호
    • /
    • pp.1624-1641
    • /
    • 2015
  • Instantaneous relay (relay-without-delay) using interference alignment is a promising approach to neutralizing interference and improving system capacity. In Wang Chenwei's work, a 2-user scenario required both source and relay to access the global channel state information (CSI). This paper shows a new method of interference alignment improves the degrees of freedom (DoF) prominently for the 3-user MIMO interference channel with instantaneous relay. This new method is focused on the relay node that completes the alignment interference neutralization so the global CSI is obtained only once and the pressure on the base station can be mitigated. In addition, the 3-user MIMO interference channels with instantaneous relay can achieve 2M DoF when source and destination have M antennas, respectively. This method shows 33% improvement over the conventional method using interference alignment which obtains 3M/2 DoF.

Efficient Inter Prediction Mode Decision Method for Fast Motion Estimation in High Efficiency Video Coding

  • Lee, Alex;Jun, Dongsan;Kim, Jongho;Choi, Jin Soo;Kim, Jinwoong
    • ETRI Journal
    • /
    • 제36권4호
    • /
    • pp.528-536
    • /
    • 2014
  • High Efficiency Video Coding (HEVC) is the most recent video coding standard to achieve a higher coding performance than the previous H.264/AVC. In order to accomplish this improved coding performance, HEVC adopted several advanced coding tools; however, these cause heavy computational complexity. Similar to previous video coding standards, motion estimation (ME) of HEVC requires the most computational complexity; this is because ME is conducted for three inter prediction modes - namely, uniprediction in list 0, uniprediction in list 1, and biprediction. In this paper, we propose an efficient inter prediction mode (EIPM) decision method to reduce the complexity of ME. The proposed EIPM method computes the priority of all inter prediction modes and performs ME only on a selected inter prediction mode. Experimental results show that the proposed method reduces computational complexity arising from ME by up to 51.76% and achieves near similar coding performance compared to HEVC test model version 10.1.

도시주거지역 도로교통소음 평가에 있어서 청감실험의 이용에 관한 연구 (The Use of Psycho-Acoustic Method on the Evaluation of the Road Traffic Noise in the Urban Residential Area)

  • 국찬
    • 한국음향학회지
    • /
    • 제12권3호
    • /
    • pp.43-48
    • /
    • 1993
  • This study was carried out to verificate the possibility of using the laboratory setting psycho-acoustic experiment compared with the field and to delineate the appropriate indices in evaluating the Noisiness of road traffic noise by means of psycho-acoustic method. Reviewing the typical patterns of traffic noises depending upon the shapes ad conditions of the road, the road traffic noises in several representative points in the major residential areas in Kwangju city were recorded and reproduced with the noise levels modified in various steps. With these 20 sound sources, psycho-acoustic experiments in the laboratory were performed on 11 volunteer subjects. And then, psycho-acoustic experiments in the real field were performed on 10 volunteer subjects to compare the results of the laboratory experiment, the results are summerized as follows : 1. The psycho-acoustic experiments in the laboratory elicited the data well matching with those obtained in the field, resulting in even higher corelation levels. This indicates that the field assessement of responses to the noise can be replaced by the evaluatioin in the laboratory settings which render many variables easily controlled and that the responses of the residents to the noise can be easily predicted in the laboratory by applying this method. 2. Also among the complex indices, such as Noise Pollution Level or Annoyance Index high correlations were detected. On the other hand, low corelations were noted among Traffic Noise Index. 3. Highly significant correlations were found among the direct indices such as Leq, L\sub 10\, On the other hand, low correlations were detected among L\sub 50\.

  • PDF

Analysis of dynamic behavior for truss cable structures

  • Zhang, Wen-Fu;Liu, Ying-Chun;Ji, Jing;Teng, Zhen-Chao
    • Steel and Composite Structures
    • /
    • 제16권2호
    • /
    • pp.117-133
    • /
    • 2014
  • Natural vibration of truss cable structures is analyzed based upon the general structural analysis software ANSYS, energy variational method and Rayleigh method, the calculated results of three methods are compared, from which the characteristics of free-vibration are obtained. Moreover, vertical seismic response analysis of truss cable structures is carried out via time-history method. Introducing three natural earthquake waves calculated the results including time-history curve of vertical maximal displacement, time-history curve of maximal internal force. Variation curve of maximal displacement of node along span, and variation curve of maximal internal force of member along span are presented. The results show the formulas of frequencies for truss cable structures obtained by energy variational method are of high accuracy. Furthermore, the maximal displacement and the maximal internal force occur near the 1/5 span point. These provide convenient and simple design method for practical engineering.